Nightmare Fuel / The Plague Dogs
The animated film is Grave of the Fireflies with puppies. See it here.

  • The relentless realism prevents you from laughing it off unless you are a sadist.
  • Two stray dogs had been captured and sold to a laboratory which experimented on animals. One of these experiments for some reason involved dropping a dog into a pool full of water and timing how long he was able to paddle until he started drowning. That's the first shot of the movie!
    • The scene in which the dogs sneak out of the lab to escape shows the walking past the other animals who were having horrible experiments done on them. Most shocking of all were the monkeys that appear to have been locked in 'rape racks'. Don't even think about looking up what they are if you haven't heard of them before. However, the devices more closely resemble non-human primate restraint chairs(which laboratories to this day still use.) The fact the monkeys have electrodes planted into their heads makes it unlikely they are being used for breeding, but equally horrific invasive brain research.
  • There's a scene where a nice man, going hunting, befriends one of the starving dogs only to accidentally shoot himself in the face. Quite messily.
    • The creepy music that plays as the man bleeds to death.
  • The film doesn't shy away from gore in the slightest. The most infamous scene above aside, the dogs graphically kill and eat lambs — the nightmarishly detailed carcasses visible after — and eat the corpse of a man sent to kill them after the Tod trips him off a death, with a good closeup of the half-eaten corpse afterwards.
  • Plague Dogs was also subject to Misaimed Marketing, with a cheerful cover of cute dogs featuring the tagline "Escape to A Different World... And Share The Adventure Of A Lifetime".