- The series is based around Gothic Horror, so it shouldn't be a surprise that this series would bring up some disturbing moments naturally.
- Zorne's conversation theme, "Sinner", composed by Unlimited Hellest. The church organs, evil Ethereal Choirs and violins, and heavy metal bass playing in the background while you're talking with Zorne may give some players chills. And to top it all off? Not only is it Darker and Edgier than her ''Rosenkreuzstilette'' stage theme and her Rosenkreuzstilette Freudenstachel stage theme, but it's even darker than several other dialogue themes.
- The Grolla Stage is set in the dark outskirts of the country, eventually leading into a catacombs and a cemetery, teeming with the undead complete with Ominous Latin Chanting.
- As Grolla Stage just wasn't enough in the first game, her Rosenkreuzstilette Freudenstachel version boasts several more Ghost 'n Goblins references, including a familiar Astaroth mini-boss. Oh, and a bloody rainstorm near the end.
- Also in Rosenkreuzstilette Freudenstachel, her boss fight at the end shows a Ghost 'n Goblins reference showing Lucifer in the background. His appearance is revealed even more by the time Grolla Turns Red.
- In the trial version updates and in the final product of Rosenkreuzstilette Freudenstachel, her stage background music is "Acid Rain" by Sound Prison, which is much darker and foreboding than her background music from the first game.
- Not to forget, a vision of Raimund possesses Grolla through her Grollschwert in the same game. In the Story Mode, you can even see her shedding a tear of pain because of her possession.
- In Sichte's stage of Rosenkreuzstilette Freudenstachel, there's "Medusa Head" ersatz floating around that's more creepier-looking than any Castlevania game.
- Schwer-Muta Casasola Merkle, full-stop. She's a Creepy Child and misanthrope with an antisocial nature who distrusts others and will stop at nothing to kill anybody who breaks their word.
- The Cross Wall in the first game, complete with blood-red tint, is sure to give you nightmares every time you fight it. Not just because it's That One Boss, but also because of its bloody tint.
- Not only is Raimund as The Grim Reaper pretty frightening in looks, but every time he enlarges his Grollschwert and has it rain down on your head, be prepared to get an expression of horror on your own face.
- Graf Michael Sepperin's demonic One-Winged Angel form is frightening enough to make your hair stand on end. This was a given considering he's an expy of Dracula.
- Sepperin Stage II in the first game. Blood dripping from the ceiling? Check. Blood running down the pillars? Check. A pool of blood? Check. The statue in the backdrop of the Poltergeist boss fight crying Tears of Blood? Double check.
- He comes back from the dead in Rosenkreuzstilette Freudenstachel, with his Story Mode portraits sports hellish red eyes that's even freakier than before. And if you thought his giant demon form in the first game was scary, his demon form has gone Up to Eleven as he's a HUMONGOUS screen-filling demon this time around. It doesn't help that he also an instant death attack when turning red.
- In Iris Stage II in Spiritia's side of the story in the first game, as you get near the end of the stage, you see a beholder (a giant floating eyeball) observing you from the background. When you get to the boss room, Lilli, with a nervously scared expression on her face, comments that the beholder has been staring at you for quite a while. As Tia says that Iris must be watching them from her ballroom through the beholder, suggesting that it serves as her palace's main camera, just then, Lilli warns Tia that something is behind her, and just as the large door closes behind her, what does what's behind her turn out to be but pieces of the stage's boss, the Deviled Egg! Think that's creepy enough? Just wait until you see the forest background behind the windows turn different shades of red as the boss fight starts after the door closes, complete with a creepy heartbeat sound.
Lilli: "Tia, that eyeball's been staring at us for quite a while..."
Spiritia: "It's got to be Iris. I'm sure she's watching us, wherever she is..."
Lilli: "...! Tia, behind you!"
- Eifer Skute. A creepy and emotionless girl with Hellish Pupils, Creepy Monotone, and a Dissonant Serenity minus the smile. Just the blank expression on her face is already unsettling. Let's not forget her violently-fluctuating emotions and mood.
- Her Schwarzkreuz stage is a very Bleak Level, complete with gloomy scenery and even gloomier Ethereal Choirs for the background music. Her stage is also filled with the undead, with a ghastly ersatz of "Mothraya" from Mega Man 4.
- Then her other stage with Iris Stage II. It is MUCH worse than Sepperin Stage II from the first game as there are statues crying blood just about everywhere you go, making it one of most gornographic stages of Rosenkreuzstilette Freudenstachel. It gets even worse still you have to swim through the blood to advance, and if the Tackle Fire ersatz even touches that the blood, it turns into a sea of intensely hot blue flames — the heat from the flames will kill you instantly!
- And finally, the reason why Eifer is such a creepy girl in the first place. At the end of Iris Stage II, it turns out that she is a clone of Freudia created by Iris. Afterwards, Eifer unleashes her One-Winged Angel form, one resembling the Shadow Devil from Mega Man X5; and after her defeat in Story Mode, we get to see Eifer crying her artificial heart out, shedding Tears of Blood.
- The Refraktia Stage has some pretty scary stuff as well. You just have to check out the wraiths floating around, and not to mention everything turning completely dark except for you, because you are the only one the light is shown around in a circular shape. Plus, the way Lecht & Rink attack is pretty scary as well. And did we mention that Rink was actually already been dead all along?
- The Pamela Stage's Game Over screen picture. She's supposed to represent Adol Christin, but the picture only manages to make her look REALLY creepy.
- Iris Sepperin. You'd think that a sweet and innocent girl who was taken captive by a dragon wouldn't have an dark side? Well, you are dead wrong. Iris Zeppelin is evil. She single-handedly orchestrated an all-out war with the Magi against the Empire, imprisoned Liebea's brother for knowing too much about her plans, pitted Spiritia's friend's against each other in life-or-death confrontations, and mercilessly kills a priest in her Rosenkreuzstilette Grollschwert opening. And for what reason she would commit such horrible, inhuman acts? Being one who may have inherited Rosenkreuz's immense knowledge, she grew tried of knowing just about everything about this world and wants to alleviates her boredom by starting this war — just for laughs.
- The way she kills her own father because he was useless in her eyes makes you want to scream bloody murder. And what happens next? She attacks your character! As Spiritia, the attack reduces her vitality to 1, no matter how much HP she has remaining! Yikes!
- Just imagine what it would be like to have a child, who you know and loved for many years, suddenly shows their horrifying true nature by killing you in cold blood out of the blue, never knowing the monster inside them.
- Even after being brought back from the dead, Graf Sepperin was still willing to protect Iris — he was still willing to protect his murderer, oblivious to the truth.
- The first form of Iris' mech in the first game. It has blood-covered rocks and crystals on its underside with sharp, bloody teeth protecting its canopy.
- The second mechanical fortress she pilots in Rosenkreuzstilette Freudenstachel is just as creepy. The first form looks exactly the same... and then you get to its second form, which resembles a giant skull with blood-stained spikes on the bottom.
- The way she sometimes laughs after killing you during the final battle of the first game.
- Freudia's ending in Rosenkreuzstilette Freudenstachel is either this or the best way to finish off a monster. Freudia freezes Iris for good. After seeing Iris crying out in her final moments, it makes one wonder who's cold-hearted here.
Nightmare Fuel / RosenkreuzStilette
Ah, RosenkreuzStilette, a Japanese doujin game series that brings so much nostalgia to many gamers (especially to fans of Mega Man and Castlevania), but who would of guessed that such a nostalgic series would also bring up some terrifying moments. Beware of heavy story and character spoilers below