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Nightmare Fuel: Resident Evil Gun Survivor
In the first Survivor, you learn from a file that Sheena Island, the mass production plant for "Mr. X"-style Tyrants, has achieved this role by making use of a hormone found inside of teenagers' brains, and it's produced when they're frightened/stressed. Umbrella knows maximum fright/stress levels are caused by excruciating pain. Yes, Umbrella tortures kids to death in order to further their goals. Just let that sink in.
Worse, they open their skulls while ALIVE AND FULLY CONSCIOUS in order to get said hormones.