- Most notably the eerie drones that end "Pree-Sisters Swallowing a Donkey's Eye", the Squickfest that is "Rubby Bulbs", and especially the climax of "Sailing Through".
- A lot of their lyrics are just... crazy. Like this from "The King of Carrot Flowers Pt. 1":
And your mom would stick a fork right into daddy's shoulder
And dad would throw the garbage all across the floor
As we would lay and learn what each other's bodies were for
- IIII LOOOOOVE YOUUUU JEEEEEEEEZUUUUSSSSS CAHHHHRIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIST
- The eerie, low-pitched singing saw drones that open "Two Headed Boy Pt. 2".
- "Sailing Through" ends on a Careful With That Axe moment that actually made one of my friends - a hardened 4chan addict, by the way - turn white. It is utterly harrowing.
- The first guitar strums in "Oh Comely" are innocent enough until you've heard the song through a few times. Then they become the most terrifying sounds imaginable. "I will be with you when you lose your breath," indeed.
- All of "Pree Sisters Swallowing a Donkey's Eye." Starts out with noisy chaos, then descends slowly into Nothing Is Scarier as the instruments fade.
- Not to mention that "April 8th", the song that precedes it, has an extremely ominous feel and transitions into "Pree Sisters" without warning.
- "Invent Yourself A Shortcake" is an early set of Neutral Milk Hotel recordings that was self-released in limited quantities during the early '90s. Much of it consists of field recordings and sound experiments done by Jeff Mangum, many of which are quite unsettling.
- "Where You'll Find Me Now" ends with a distorted sample of carnival music. Kind of cliché, but still creepy if you're hearing it for the first time.
- The ending of "Communist Daughter" is particularly chilling if you happen to be listening to it at night.