This is based on opinion. Please don't list it on a work's trope example list.
Nightmare Fuel / Earth vs. the Spider
The scene where a toddler is the sole survivor of the spider's attack. Rather chilling and a first for Hollywood.
The opening bit, where the dad is probably getting sliced up by the web, is also pretty effective.
Not to mention the father's corpse when they do find it. The effects person deliberately wanted to invoke the image of a "living Egyptian mummy."
The incredibly obscure trailer you can only find in the VHS tape 'Fantastic Dinosaurs of the Movies'. It's short, but dear god, the narrator's voice cracks to the same level as the normally silly roars of the spider. Make of that what you will...