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* In ''WesternAnimation/BatmanUnderTheRedHood'', this is Ra's al Ghul's reaction when he realizes that ComicBook/TheJoker killed Jason Todd - he just wanted the Joker to ''distract'' Batman, not take a young man's life.
* In ''WesternAnimation/TheBookOfLife'', Xibalba doesn't exactly say it, but the moment La Muerte angrily reminds him of the last wager he cheated on, he goes quiet. And looks at her with regret in his eyes and body movement.
* ''WesternAnimation/{{Brave}}'':
** Queen Elinor has a minor one when in an argument with her daughter Merida, she tossed Merida's treasured bow (a gift from her father) into the fireplace, causing Merida to run away sobbing. After Merida leaves, the bowstring makes a loud snap in the fireplace, causing Elinor to realize what she has destroyed - she even tearfully whispers "what have I done?!" She manages to fish it out, but it's too late. It's ruined, and so is her relationship with her daughter.
** Later in the movie, Elinor gets this again when [[spoiler:she reverts into the mind of a wild bear, and accidentally hurt her daughter and also her husband.]]
** Merida has this herself from the point where [[spoiler:she accidentally transforms Elinor into a bear]] until the end of the film. But it is only evident in the last few minutes, [[spoiler:where it appears that Elinor has reverted to the wild bear state, and will never come back]].
* ''Disney/BrotherBear'': [[spoiler:Kenai has this feeling when he realizes that the bear he killed was Koda's mother. When he tells Koda about what happened, Koda doesn't take it well.]]
* In ''WesternAnimation/{{Cars}}'' Doc Hudson after [[spoiler: calling the press to take Lightning away to compete for the Piston Cup; Sally calls him out on his selfishness and everyone turns their backs on Doc as he is left alone at the broken traffic light.]]
-->'''Sally''': You called them?\\
'''Doc''': It's best for everyone, Sally.\\
'''Sally''': Best for everyone... or best for ''you?'' [''drives off'']
* Gill from ''WesternAnimation/FindingNemo'' goes through one for nearly getting Nemo killed in one of his escape plans. He even apologizes for it face-to-face with the kid.
-->'''Nemo''': Gill, I'm sorry I couldn't stop the--\\
'''Gill''': No, kid, ''I'm'' the one who should be sorry. I was so ready to get out, so ready to taste that ocean, I was willing to put you through harm's way to get there. Nothing should be worth that. I'm sorry I couldn't get you back to your father, kid.
** In ''WesternAnimation/FindingDory'', Dory unwittingly ignores Marlin's pleas to keep her voice down around a dark area, and she, Marlin, and Nemo get chased by a giant squid that nearly eats Nemo. When Dory sees how shaken up Nemo was, she's horrified at what had happened, forgets what happened, and remembers when she sees Marlin comforting his son, murmuring apologies when Marlin gets angry at her.
** After that, Marlin eventually regrets angrily telling Dory that forgetting was "what you do best".
* At the climax of Disney's ''Disney/TheFoxAndTheHound'', Tod saves Copper from [[BearsAreBadNews a huge grizzly bear]], and barely survives falling down a waterfall with it, leaving the fox, weak and exhausted, collapsing at the riverbank. Copper approaches, amazed Tod--the very fox he tried to hunt--saved his life, despite everything that happened, and now feels genuinely remorseful for what he's done to him. [[spoiler: Then he steps in between Amos' gun & Tod refusing to move away, and Amos lowers his gun.]]
* In ''Disney/{{Frozen}}'', Elsa says this almost word-for-word when [[spoiler: she is locked in a dungeon and looks out the window to see the endless winter she has (inadvertently) caused.]]
* In ''Film/{{G Force}}'' [[spoiler: Speckles]]. says almost word-for-word after realizing that [[spoiler: he nearly destroyed the ones who truly care about him.]].
* In ''WesternAnimation/{{Home}}'', [[spoiler:Oh, when he realizes how wrong his race was to invade the Earth, and then later that Gorg was only after the Boov because they stole his species' entire next generation]].
* In ''WesternAnimation/{{Hoodwinked}}'' the wolf utters this phrase after [[CaffeineBulletTime giving coffee to]] his hyperactive squirrel partner so that he can run quickly and tell the police that [[spoiler: the rabbit is the villain]].
* ''WesternAnimation/HowToTrainYourDragon'': Stoick gets one of these after he [[IHaveNoSon disowns]] Hiccup for fraternizing with a dragon, [[spoiler:and when he realizes that Hiccup was telling the truth about the Green Death and he's just doomed everyone with him.]]
* During the climax of ''WesternAnimation/InsideOut'', Anger gets a particularly painful one after realizing that [[spoiler: the idea he's given Riley to run away back to Minnesota has triggered an all-consuming depression that he, Disgust, and Fear are powerless to stop]].
-->'''Fear:''' Guys, [[spoiler:we can't make Riley feel ''anything'']].\\
'''Anger:''' ''[[[OhCrap beat]]]'' What have we done?
* In ''WesternAnimation/JusticeLeagueDoom'' this is the reaction of Batman when he realizes ComicBook/VandalSavage stole and is implementing his [[CrazyPrepared contingency plans]] to [[http://www.youtube.com/watch?v=GhLfvRogiTk defeat the other Leaguers.]]
* King Triton says this almost world for word in ''Disney/TheLittleMermaid'', when he believes his argument with Ariel and destroying her human artifacts led to her running away. The look on his face immediately after said occurrence when she's reduced to tears can count as well.
** Sebastian too after he "betrayed" Ariel after accidentally blurting out her love for a human to the king, which lead to the aforementioned incident.
** Ursula had her moment after learning in shock that she killed her pet eels Flotsam and Jetsam with the trident by accident (thanks to Ariel yanking her hair), even mourning for their deaths before turning her rage towards Ariel for it.
* ''WesternAnimation/{{Madagascar}}'':
** Kind of a "My God, What Did I Almost Do?" moment with Alex [[spoiler:after he almost kills Marty, who is luckily saved by Maurice dropping a coconut on Alex's head, which then leads up to Alex saying, "Oh no. What have I done?" and then [[HeelRealization realizing he's too dangerous to be around his friends, including Marty]] and deciding to leave for their own safety.]]
** Marty says this word for word when he realizes that his bringing his friends into the wild [[spoiler: caused Alex to almost revert to his predatory instincts and leave them.]]
* ''WesternAnimation/{{Megamind}}'' thinks this after seeing [[spoiler:Hal/Titan]] using his powers for his own purpose. Instead of creating a new hero for Metro City, he made a villain far worse than him.
* In ''Mickey's Twice Upon a Christmas'', Mickey has this reaction when he realizes that him punishing Pluto for messing up his decorations led to him running away.
-->'''Mickey:''' Oh Pluto, if only I hadn't yelled at you. I swear I'll never raise my voice again.
* In ''WesternAnimation/MonstersInc'', although he doesn't say the actual words Sulley's reaction to accidentally terrifying Boo during his scaring demonstration, and by extension the other human children he has similarly terrified as the monster world's 'top scarer', is basically this.
-->'''Sulley:''' Did you see the way she ... ''looked'' at me?
* [[spoiler: Twilight]] has a massive moment of this in ''WesternAnimation/MyLittlePonyTheMovie2017'' when the pressure and stress of their situation causes her to lose her temper and shout [[spoiler:[[WhamLine "Maybe I'd be better off without FRIENDS LIKE YOU!"]]]] No one, least of all herself, can believe she actually said that.[[note]]She IMMEDIATELY realizes how badly she screwed up, but the damage is done.[[/note]]
* In ''WesternAnimation/TheNightmareBeforeChristmas'', after being blown out of the sky for impersonating SantaClaus, Jack laments "What have I done?" at the start of the song "Poor Jack."
** Interestingly, the second half of the song subverts this. Jack starts off miserable, then turns bitter ("Why does nothing ever turn out like it should?"), then suddenly triumphant ("Well, what the heck?!/I went and did my best/And by God, I really tasted something swell!") before rushing off to fix his mistakes at the end.
* In ''WesternAnimation/ParaNorman'' [[spoiler: Judge Hopkins and the other zombies have this after they first died. As zombies they realize they were just foolish and scared of an innocent little girl who could talk to the dead, so they spend the movie trying to make up for it. Even at the end of the movie, their spirits still look regretful for [[MoralEventHorizon what they did.]]]]
* In ''Disney/{{Pocahontas}}'', Thomas has this moment during the VillainSong "Savages" after he shot Kocoum to save John Smith's life. Not only does John Smith [[TakingTheHeat takes the blame for the murder]], as well as sentenced to death at sunrise, but it caused war on both the Powhatan Tribe and the Settlers. His look on his face pretty much says it all.
* In ''WesternAnimation/ThePrinceOfEgypt'', Moses accidentally pushes a slave overseer off a scaffolding to his death whilst saving an old Hebrew from being beaten brutally. He immediately has that reaction before deciding to exile himself out of guilt.
* Cellach, in ''WesternAnimation/TheSecretOfKells''. [[spoiler: As the sacking of Kells begins, it begins to get through to the Abbot that, despite all his preparation, things aren't going to work out. And what follows is a massive slaughter that basically makes the rest of his life one really prolonged version of this trope.]]
* ''WesternAnimation/Shrek2'' Fiona's father, King Harold Harold has this after Fiona cut her parents out of her life when she's fed up with their prejudice towards her and Shrek. He realizes how selfish he'd been to be so focused on getting rid of Shrek (hell, he thinks Shrek is dead at this point because he put a hit on him, not knowing the hit failed) and didn't take Fiona's feelings into consideration. He has this again when Fiona casts him a disgusted glance after Shrek changes (though in fact it is Charming pretending to be Shrek) and when Harold is forced by the Fairy Godmother to slip Fiona a love potion after he realizes Fiona has no interest in being with anyone but Shrek. He has a change of heart last minute, deciding not to give Fiona the love potion after he realizes how wrong it was to impede upon her free will.
* ''WesternAnimation/{{Sintel}}'': The main character has spent years searching for her missing friend, a baby dragon, and killing the adult dragon that kidnapped it. [[spoiler: Sadly, [[TimeSkip more years had passed than she realized]], and she ends up killing her now adult dragon friend by mistake]].
* In ''WesternAnimation/SouthParkBiggerLongerAndUncut'', Creator/ConanOBrien turns in Terrence and Phillip to [[MoralGuardians Mothers Against Canada]]. When Terrence and Phillip remind Conan that he laughed at their movie and that he's "A very bad man" for betraying them, Conan shouts "What have I done?" and tosses himself out a window.
** Shiela has one herself when she [[spoiler: [[MoralEventHorizon guns down Terrence and Phillip,]] [[ApocalypseHow thus causing Satan and Saddam to rise.]] This in turn causes her HeelFaceTurn at the end.]]
* ''WesternAnimation/{{TMNT}}'' has one of these with Raphael in his battle against Leonardo, which is luckily averted just in time. [[spoiler:Raph was dangerously close to killing Leo after a fiery battle, under the impression that Leo left him to be a show-off; the realization causes him to run away in fear.]]
* In ''WesternAnimation/ToyStory'', Slinky paraphrases this when he and the other toys see Woody and Buzz riding toward the moving truck and realize Woody was telling the truth. Downplayed with this PlayedForLaughs line:
--> '''Rex:''' "Great, now I have guilt."
* ''Disney/WreckItRalph'':
** When the characters of Sugar Rush realize who [[spoiler:Vanellope really is, everyone panics, especially the other racers. Of course, they never realized the truth since their code had been manipulated by King Candy/Turbo.]]
** Ralph gets his own, [[RuleOfThree threefold]]: once when [[spoiler: he smashes Vanellope's car on King Candy/Turbo's request]], again when he realizes his game-jumping will cause his game to [[spoiler:become unplugged since now Felix has gone after him and hasn't returned]], and finally when he realizes that it is his fault that [[spoiler:the Cy-Bugs from ''Hero's Duty'' have invaded ''Sugar Rush'']].
* ''Disney/{{Zootopia}}'':
** After Judy says at a press conference that [[spoiler:predators going savage is in their nature, submerged predator/prey tensions rise to the surface causing civil unrest, and Nick is left feeling hurt and betrayed leading to their PlotMandatedFriendshipFailure]]. Seeing all this causes Judy to regret her ignorant remarks.
** When Chief Bogo demands that Judy surrender her badge, Nick is seen looking surprised and guilty as he sees the consequences of his wasting Judy's time. This leads him to give Chief Bogo a WhatTheHellHero speech where he reminds him that they still have ten hours left and still have a case to solve.