A Spinoff of A Certain Magical Index.Mikoto Misaka is the third-most-powerful Esper in Academy City, a state-of-the-art city dedicated to the scientific study and development of Psychic Powers. Every student in Academy City is given an "Esper Rank" to indicate their power, and Mikoto is a Rank 5: the best of the best, an electromaster with the nickname of "The Railgun".Together with her friends in "Judgement" (the student-run "Public Moral Committee"), she works to protect the peace in Academy City from delinquents and hoodlums. The story narrates the events of A Certain Magical Index from Mikoto's perspective, as well as delving into her own conflict with the darker evils that lurk in the shadows of Academy City.A Certain Scientific Railgun is a manga spin-off and supplementary reading to the A Certain Magical IndexLight Novel series written by Kazuma Kamachi. Similar to the original, it has received an Animated Adaptation courtesy of JC Staff, which covers the first major arc of the manga as well as some anime-original material penned by the author. In addition, there are side-story novels which involve the protagonists with the Magic side.This series shares a character sheet with A Certain Magical Index.The anime is being licensed by FUNimation entertainment, along with A Certain Magical Index.A second season, A Certain Scientific Railgun S, aired in Spring 2013, focusing on the Railgun version of the Sisters Arc and an anime-original plotline.Also, check out the franchise's other spinoff, A Certain Scientific Accelerator.Has a recap page.A note on timelines: The manga's events begin two weeks before the Index novel and anime. The anime, however, violates this by adapting events from Volume 8 of the novels into the anime's first episode. As it stands, Chapters 18 to 39 occur around the time of Volume 3 in the novels, Chapter 40 is around Volume 5, Chapters 41-42 and around volume 8 and Chapters 43-current take place around Volume 9.
These works provide examples of:
A-Cup Angst: Mikoto is very self conscious of the fact she is not as... "developed" as other girls her age, if not younger.
Ascended Meme: Heaven Canceler's Fan Nickname, Dr. Gekota, is acknowledged when Saten mentions how the doctor looks like Gekota.
In the manga, Saten was shorter than Mikoto and had a flat chest. In the anime her height is sometimes the same as Mikoto's. Her bust in some official art (when she is in a bikini) is increased to a C cup. JC Staff really likes her. Exhibit A◊, Exhibit B◊.
JC Staff is really generous with these upgrades, especially about the eyes. Shinobu doesn't look like a Deep One and Misaka Sisters' look a lot less dead.
The anime gives more character development to the cast from the Railgun manga and even explains more details about Academy City and the people who live there. Furthermore, It continues where Level Upper arc left off and gives it a proper end.
The entire series is essentially this, especially Railgun's version of the Sisters arc, which explains Mikoto's struggles against the Level 6 Experiment in more detail compared to the Index version. It also shows a more introspective side of Accelerator, which makes his Face-Heel Turn more understandable.
Adult Fear: What made the tragedy of Professor Kiyama Harumi so easy to relate to for adult audiences is the very human fear in every mother that society would hurt, betray and take their beloved children away from them. This infuses her quest to save her students, extreme measures notwithstanding, with a sad and admirable nobility that you can't help but root for.
Affably Evil: Frenda, Mugino, Rikou and Saiai act like your normal everyday teenage girls except when they do mercenary work as Team ITEM, they are damn good.
All Myths Are True: The urban legends talked about in Episode 4 all turn out to actually be true, including the Imaginary Numbers District, Toma's Imagine Breaker and the Level Upper.
All Part of the Show: In the Railgun Side Story: Liberal Arts City, a battle between Mikoto and a Mixcoatl piloted by Xochitl is passed off as a show to everyone else, despite the danger being very real. Once things get completely out of hand later on, they couldn't keep it up.
Alpha Bitch: Considering Tokiwadai is a school for girls of the elite rich, there are bound to be some Alpha Bitches at that school.
Kongoh is a bit of a subversion. When first introduced she acted like a stereotypical Alpha Bitch with Hidden Depths, before her hidden depths began being more expanded on and she genuinely starts helping people and always comes to the defense of her friends. In the second half of the second season, she even asks her father to use their airport's Humongous Mecha as a battle vehicle against STUDY! She became a full Lovable Alpha Bitch with every single appearance.
Kuroko acts like the Alpha Bitch towards those she see as below her and does it unintentionally sometimes.
Averted with Mikoto. Despite coming from a rich family and being just as famous as Misaki, grew up like a normal girl thanks to her mother and acts kind and friendly to all people, no matter what social class they come from.
Amusing Injuries: Kuroko would not have survived getting her darned neck broken by the dorm supervisor in Episode 2 if it wasn't ''so darned funny.'' Same thing when Mikoto punishes her in general for being too perverted at the time.
Anti-Villain: Professor Kiyama, whose motivation of saving children who were her students rendered comatose by a corrupt government experiment is noble enough to earn Mikoto's sympathy and offer to help her accomplish it.
Arbitrary Maximum Range: Even used as a plot point, the title-named character's signature move seems goes from "would wreck a tank" to "bounce off harmlessly" exactly at 50 metres. The exact number is artistic license, but it instantly going from lethal to harmless is justified due to the coin melting.
In reality, lightning travels extremely fast (about 92,000 miles per second, or 148,000 kilometers per second) so dodging or blocking Misaka's attacks should probably be out of the question. That's just the first one. This series isn't about the clash of Magic and Science, but the clash of Magic and Techno Babble. Given it opens with a acknowledged psychic powers, you should really expect that sort of thing.
Misaka's odd ability to completely ignore the shockwave and recoil of firing her "Railgun" out of her hand, which should at the least rupture her eardrums(if not outright kill her) and shatter the bones of her arm every time she uses the power.
Also, Misaka herself comments on the fact that she is limiting her power just so they can get a reading in the first episode. The people administering the test are probably too scared by her power to notice anything odd.
Justified - the entire premise of esper powers is that espers can subconsciously alter reality and substitute their own rules into it.
Ascended Extra: Saten and Uiharu. In the manga, while they were very prominent during the Level Upper arc, they have extremely minimal appearances in subsequent ones. Even Shirai doesn't have nearly as many appearances in the manga as she does in the anime.
Bait the Dog: In the manga, two scientists are around when 9982 cleans up. They joke and laugh and 9982 criticizes the tea. All very friendly and affable. Oh, by the way 9982, don't forget to clean up the half dozen bloody corpses of your sisters before you change into the uniform, or you're just going to have to do it again. Toodles!
Barely-There Swimwear: Kuroko's in Episode 13. Mikoto was not amused. Pops up again in Liberal Arts City.
Touma blocked the Graviton Bomber's power, saving Uiharu and a little girl, as well as Mikoto.
Given Kuroko's job and ability to appear completely out of the blue, she pretty much does this trope on a daily basis. Mikoto is so aware of this that in one occassion, she was actively counting on Kuroko to do this.
Not to be outdone, previous Butt MonkeyKongo Mitsuko pulls this off twice. The first time was, unfortunately off screen. The second time she knocks two helicopters out of the sky by blasting a truck at them. Not bad.
In Episode 24 of season 2, several characters pull these off. First, Konori brings Judgment reinforcements to prevent STUDY's robots from leaving the assembly area, then Kongo's dad brings a Humongous Mecha to the area to counter STUDY's mechs, and Mugino saves Mikoto from some robots utilizing her Meltdowner powers.
Big Eater: Index's and Himegami's respective appetites haven't vanished even while cameoing over to this series.
Big Ol' Eyebrows: The reason for Miho attacking Tokiwadai students in the first place.
Black Bra And Panties: Saten rummages through Kuroko's underwear drawer, pulling a progression of more and more racy items. It starts with a pair of these, and ends with full-body fishnet stockings.
A brief shot in Episode 10 (when the characters are discussing computer networks as an analogy for how Level Upper might work) reveals that Konori uses a "Macindows" laptop equipped with an "Outel" processor.
And then averted when the Dorm Supervisor is seen going into a Pizza Hut.
Boke and Tsukkomi Routine: Much of the humor in Kuroko and Mikoto's friendship stems from this: the former says or does something spectacularly stupid/perverted, and the latter rewards it with a hard knock to the head.
Book Ends: In the beginning of the first episode of Railgun, Mikoto is humming at a bridge. The last episode ends with Mikoto at the same bridge but now with her friends Kuroko, Saten and Uiharu watching a blimp with a birthday message to Kiyama.
Brandishment Bluff: In the second episode of Railgun S, a mysterious girl that has been distributing cash cards in alleyways does this to a gang of Skill Out members who try to rob her. She pretends to have an esper ability that allows her to kill anyone she touches with a thought in order to frighten them into leaving her alone.
The Beach Episode in Episode 13 provides a much-needed break after the close of the Level Upper arc.
Episode 19 is Lighter and Softer compared to the final arc in the first season that immediately follows it.
Episode 7 of season 2. Although Misaka starts attacking labs that were responsible for the Sisters Project, it largely takes place offscreen, and instead the focus is on Kuroko, Saten, and Uiharu.
Subverted with Episode 11 of Season 2. It starts out as one, with Mikoto kicking the vending machine that ate Touma's 2,000 yen bill, but then Mikoto sees yet another Misaka sister, who informs her that the project is still ongoing, and that one took place that morning. She doesn't take it very well when she learns there are at least 183 other facilities which are now involved with the sister's project.
Episode 17 of Season 2, which focuses on a hotpot party with the four main girls, with Mikoto running into Frenda and Mugino in a family restaurant. That encounter, while not exactly friendly, is on a very comedic level as Kuroko and Frenda get into a heated argument about whether Mikoto or Mugino would win in a fight, with both girls inadvertently revealing embarrassing details about the two.
Brought Down to Normal: Mikoto suffers this briefly in Episode 4 of Season 1 in her first real fight against Touma. She tries various attacks, only for Touma to cancel every single one of them, then tries zapping him directly by grabbing his right hand. Unfortunately for her, his right hand cancels out her power. When they both realize she's helpless, Touma makes a fist with his left hand, and she flinches in anticipation for his punch. But seeing how pathetic she looked, he decides against hitting her, and lays on the ground pretending to be beaten.
The main plot in the first half of the anime centers around a "Level Upper", which turns out to be an audio file. It works by synaesthesia, using sound alone to manipulate all of the senses at once—not, sadly, The Power of Rock—in order to effect an increase in power by networking the users together and sharing their powers with each other. Unfortunately, its users inevitably fall into comas. Here's the audiofile itself.
The real file is on the second soundtrack of Railgun as the 12th track. It is not as harsh as the one on YouTube.
In the second half of the anime, there's some importance given to another Brown Note"Capacity Down", which causes any power users to experience great pain and be incapable of calculating the necessary things to use their abilities. Good thing the resident level 0 brought a baseball bat to the last battle.
In the Sisters arc, Mikoto has to fight Frenda of ITEM. At one one point she almost loses her legs and comments that they're her pride as a woman. Guess what happens later on in the Index novels... If you guessed having her legs blown off, give yourself a pat on the back.
The AIM Burst in Episodes 11 & 12 is a more subtle Call Forward. Notice that halo on top of its head? Now fast forward to the Academy City Invasion arc. That's right, the AIM Burst is a forerunner of Fuse=KAZAKIRI.
Anything from characters to plotlines in the Railgun manga/anime is canon in the main Index series as it was made by the author of the novels. This include Saten who makes a cameo appearance in the novels and Capacity Down is used on Accelerator in Volume 19 of the novels. This also counts in the Railgun manga, where anything added in the Railgun anime is treated as having happened in the manga. Including Harumi's students being cured.
There's also Shinobu Nunotaba, a scientist formerly working on the Radio Noise Project, and Nayuta Kihara, who has already ascended to canon through Word of God.
Mikoto charges at Accelerator at the very end of Episode 5 in Season 2 after learning about the Level 6 Shift Experiment to make him a Level 6 by having him fight and kill over 20,000 sisters, and arriving just in time to witness him kill the sister she spent the day with.
Chapter 61: Gensei Kihara forces the power of the MISAKA Network into Mikoto, causing her to transform into a vaguely demonic form and ending the chapter with the line, "Now, let the experiment begin; Can Misaka-kun reach Level 6?"
Consulting a Convicted Killer: Mikoto visits her old enemy Therestina Lifeline in her cell for clues on the organization STUDY and for how to save Febri from her life-threatening position. Therestina really didn't want to help, but her advice proved very useful.
Mikoto started as an idealist but later becomes an understanding realist when she learns not everyone has the will and effort to become a higher level esper and learns to respect and be sensitive to other people's feelings especially the Level Upper users like Saten. But then it gets bad when she becomes a unbalance cynical, Stepford Smiler during the Sister arc. Things got much worse and then she got better.
Saten start as a cheerful Genki Girl despite being a Level 0. As the series progress, she gets jealous and envies her other friends who have powers which lead her to use Level Upper to finally get one but at the price of getting into a coma. After waking up from her coma and realizing there are somethings more important than powers, she becomes proud of being who she is and resolves to work harder to get the powers she had under Level Upper, the right way.
Episode 4: in a list of "urban legends", the Level Upper is mentioned. Similarly, closeups of criminals' mp3 players are seen as far back as the first episode, eventually it is revealed that the Level Upper is actually a sound file that one can listen to.
Episode 12: The camera focuses to a group of 4 girls at a restaurant and a mysterious lady who likes Margery Daw at the nuclear power plant.
Remember Episode 17? The one featuring a mysterious girl whose pendant has a picture of a certain scientist's student and the earthquake that happened in that episode? Guess who's the new transfer student at Uiharu's school and what the members of Anti-Skill and Judgment are talking about in episode 20?
Remember the Capacity Down? Yeah, we get to know who built it, and see it in use again.
Chekhov's Gunman: Saten Ruiko is a Level 0, so even with an aluminum baseball bat she can't do much in a battle with a giant mech, yet she proves instrumental in the final battle in Episode 24 as she was the only one Therestina didn't detect and the only girl in the group aside from Kiyama not being affected by Capacity-Down and manages to destroy it!
Happens to Mikoto during her first fight with ITEM, although its not done for Fanservice, but rather due to how intense the fighting gets with explosions.
The sheer power given off during Mikoto's Level 6 Shift burns all her clothing to ash. Touma covers her with his jacket.
Color Failure: Happens to Kuroko in Episode 20 of Season 2 when she enters the bathhouse, fully intending to bathe with Mikoto in all her glory. Then she sees their dorm supervisor, who then forces her to massage her. Kuroko turns white upon seeing her.
Continuity Nod: Railgun is filled with nods to the To Aru series if you look closely.
Episode 20 shows Mii on the computer, and next to it is a bin, overflowing with milk cartons. Remember that?
Episode 19 helps explain why the Tokiwadai Dorm supervisor in Index is different from the one in Railgun, explaining there are in fact 2 Tokiwadai dorms.
Remember Episode 18 when Uiharu's & Saten's teacher wanted to propose to the teacher from Cypress Park? Guess what's on his hand in Episode 20.
In Episode 15, everyone except Kuroko still haven't forgotten that Saten used Level Upper.
In Episode 6, we see a girl who is suspected of being the Graviton Bomber. She isn't. We meet her again, during the special classes for the Level Upper users.
Several characters from the Railgun anime appeared in the manga like Erii and that female gangster Mikoto fought.
A minor one. The Judgement member who saved a girl from the Graviton Bomb by knocking her down and protecting her with his own body was an instant hit with the fans. We later found out in the manga that he's dating her. Good job Hero!
Chapter 43 references Misaka's experiences during the Someone's Watching incident of the OVA.
The Railgun manga has many nods towards the side stories. In Chapter 43 Mikoto is mentioned to have experience doing demonstrations in Russia (Shopping Mall Demonstration SS), in Chapter 44.5 a disguised Xochitl runs into and remember Saten from the Liberal Arts City SS, and even more subtly, in Chapter 42 Saten makes an offhand comment about how the jeans she ordered from England haven't arrived yet (Norse Mythology SS).
Cool Airship: The Mixcoatls in Railgun SS, made of obsidian, wood, and cloth, that can out fly fighter planes.
Clap Your Hands If You Believe: Esper powers affect reality based on their users' internal reality. According to the pseudoscience of the series, if a Schrödinger's cat experiment was set up, espers would find the cat in the state that they believed it to be, because they believed strongly enough that the cat was that way. Hypnosis, brainwashing and drugs are used to throw the subjects internal reality out of whack. So, it might be argued that the strongest espers like Mikoto and Accelerator are the most stubbornly out of touch with the reality normal people live in.
Clingy Jealous Girl: Kuroko, who threatens Touma the moment she sees him out of suspicion over his relationship with Mikoto, telling him that if he wants to make a go at Mikoto he'll have to go through her first. Though despite her jealousy, she never tries to sabotage or otherwise impede Touma's conversations with Mikoto. While clingy and jealous, Kuroko has the best interest for Mikoto at heart.
Cry Cute: Mikoto in Episode 4, and Kuroko in Episode 2.
Cool Car: Kiyama's Lamborghini. Saten is also seen cross-stitching a different Lamborghini during the school festival.
Mikoto is usually on the giving end of these, although during her first fight with Therestina, she's on the receiving end of one.
Also played straight with Mikoto vs Touma fights. In one battle, she grabs his right hand and attempts to electrocute him like she's done to many other opponents. Except that it doesn't work due to his special power, and Touma makes a fist. However, the pitiful look on Mikoto's face along with her attempt to block his punch causes him to lie on the ground and declare her the winner. Which she doesn't take very well either, even after he points out why he purposely "lost" the fight.
Mugino was smart enough to leave Saiai to defend one of the research centre's while she, Frenda and Rikou fought Mikoto at the other one in case Mikoto was just a diversion. She was right.
Frenda is this in her own right. She released a mist that she claimed was harmless to humans but would detonate with the tiniest spark, effectively making Mikoto a normal. The mist was actually a bluff and completely harmless, but it worked (until Frenda screwed up anyway).
Darker and Edgier: Touma's side of the Sisters arc was one of his darkest arcs by implication of all the horrible things that had happened. On Mikoto's side, we see it all.
Date Crêpe: Kuroko gets the same flavor of crepe as Mikoto in an effort to get more intimate with her, and is horrified when Mikoto shares a bite with someone who got a different flavor.
Defrosting Ice Queen: When it comes to orphans and love, the Dorm Supervisor shows a nicer and kinder side. Too bad the love part didn't last.
Delusions of Doghood: Saten in Episode 2 of Railgun S, when tracking Urban Legends about cash cards.
Designated Girl Fight: When the scientist in charge of the Sisters Project learns that Mikoto is destroying their labs, he decides to sent in a team of mercenaries. Said team is Team ITEM, but it's a subversion as they hired not because they were girls, but because the team is composed of professional mercenaries and their leader is a Level 5.
Dude, Where's My Respect?: When the main girls run into Febri for the first time in Episode 19 of Season 2, she seems overly friendly to them, except Mikoto. Even Kongou and her friends get more respect than her. Mikoto tries to do various things to get Febri to like her as well, but other than being forced to give away her rare pink gekota finger puppet, Febri doesn't really care about her for some reason. She does however, start to slowly warm up to Mikoto in the next episode, but just barely.
Eagleland: Definitely flavor 2 in the Railgun SS: Liberal Arts City, almost to the point of being anvilicious; the author sees America (in a very stereotypically Japanese way) as the movie-obsessed land of women with huge breasts, overly large vehicles and people toting guns everywhere.
Early-Bird Cameo: The Railgun manga and its anime adaptation has characters from the later novels appearing much earlier.
In manga Chapter 1, Aiho Yomikawa makes a cameo appearance after the crime scene.
In Chapter 8, you can see Stiyl passing by Uiharu.
In Chapter 11, Mikoto & Kuroko meet a certain Frog-faced doctor.
In Chapter 15, the "Shonen Hero" in one of the Level Upper user's memories is none other than Gunha Sogiita, the seventh Level 5 esper, and in the same chapter we have the appearance of a group of girls hanging out. Those girls are none other than the members of ITEM led by the fourth Level 5 esper, Shizuri Mugino.
For all the obstacles, the setbacks, the pain and the suffering she went through and the way the city treated her beloved students, Kiyama finally got her happy ending where she is reunited with her now awakened and cured students. And it's one she truly deserves.
Mikoto goes through this quite a bit as well, and has to really work for her happy endings. Fortunately for her, although she sometimes tries to solve it herself, other times she is given a helping hand, such as Touma going to defeat Accelerator to force a stop to the Sisters project when she resigned herself to dying in an attempt to stop their deaths.
Eldritch Abomination: The AIM Burst creature after people have been shooting it, causing it to grow and deform, most notably Squick developing eyes in random places.
Ensemble Cast: In the anime, the core main characters consist of Misaka, Shirai, Saten and Uiharu and they all have ample turns in the spotlight. In the manga, the only real "main" character is Misaka. Shirai is a recurring secondary character while Saten and Uiharu aren't very important outside of the Level Upper arc.
Eureka Moment: Episode 3: The girls are stumped over why a girl shows up on camera but isn't seen by her victims, since everyone with the power to turn invisible has an alibi. After Kuroko remarks that she "didn't notice" the pigeon Uiharu saw fly by, Mikoto realizes that the girl must have the power to erase her presence from the minds of people who see her directly.
Every Girl Is Cuter with Hair Decs: All the main characters have some form of hair decs, but Uiharu is definitely overdoing it with the flower bouquet on her head. The four main girls' are highlighted in the first ending.
Evil Albino: Accelerator. Although he gets better in Index.
Evil Old Folks: Kiyama's former boss, Gensei Kihara, is a heartless scientist who sees no problem with using children for his experiments. To him, they are "ingredients" to be use in the name of science.
Fanservice: With a huge female-only cast including at least one perverse loli and skirt-chaser, you can guess there is LOTS of it. Though it manages to be self-conscious, tongue-in-cheek and good-natured.
Fantastic Racism: Though everyone in Academy City is treated with equal rights in theory, in practice your chances of getting passed over for jobs, universities and all other opportunities in life steadily increases the lower your Power Level gets. Well meaning Level 5's such as Mikoto try to reassure their "lesser" peers with ultimately hollow mantras of "Do your best!! Your power level doesn't matter!!" oblivious to the fact that certain people are simply born incapable of crossing certain hurdles no matter how hard they try. Naturally, this breeds intensely bitter resentment, especially amongst the Level 0's of the city's population; ripe for exploitation by people like Professor Kiyama Harumi through schemes like the "Level Upper" incident... Scientists such as Therestina and the rest of the Kihara Family don't even treat espers as humans; merely test subjects for their experiments.
Felony Misdemeanor: Episode 3 is about a culprit who sneaks up on girls, stuns them with a taser and while they are unconscious performs on them the depraved, heinous act of drawing them huge eyebrows. Sure, tasing someone to the point of unconsciousness is pretty bad on its own, but people are more pissed about the second thing.
Filler: Because there were only 4 manga volumes of Railgun at that time, and the Sisters arc was still ongoing, filler episodes were created after the first arc in the Railgun anime to fill in its 24 episode slot.
Flower in Her Hair: Uiharu practically wears a flowerbed on her head, while Saten wears a lone white flower. As Uiharu has both refused to answer Kiyama about what they are, and only answered "What are you talking about?" when someone pointed them out to her at the Tokiwadai Midsummer festival, they may be more than a headband, though the novels state they are artificial flowers.
Food Porn: One of the most obvious visual tics in the anime is that whenever Mikoto and her friends appear they'll probably be eating something like parfaits. No reason, apparently they're all just constantly eating.
Foregone Conclusion: The Sister's Arc in Railgun, since its events was already shown in Index. However, this time it shows the events from Mikoto's perspective, and we see what she was doing during this arc, which had only been hinted at in Index.
In the manga Kiyama gives a hint to Mikoto over where she got the idea to create Level Upper. The origin of that idea? The Sisters project. Skipped in Railgun anime which is switched to Therestina telling Mikoto everyone in Academy City is a guinea pig to reach Level 6.
Remember some of those Urban Legends the girls were talking at Episode 4? They hint about what's going to happen in the Index anime.
In the first episode of the second season, Mikoto has a very brief flashback to when she gave her DNA map to the researchers. This is explained in the next episode.
For Great Justice: The Liberal Arts City had been implied to have captured espers and experiment on them, as well as get rid of people who snoop using Secret Police, control information, and other various things to create their own espers using a different method from Academy City, based off the magicians attacking them for this reason. They claim it's so they wouldn't be behind the times as the "World Police" since it's possible that espers would become common place in the future, but they were just as bad as Academy City's For Science!!
We get a flashback of a previous incident in which orphaned children (complete with one girl talking about how grateful she was to the city for taking care of them) are used as research experiments (you see where this is going, right?) and ultimately sent into comas when put through a treatment to test pushing psychic powers to their limits. As the experiment goes terribly wrong, the head researcher stops another from calling for ambulances, saying the results are much more interesting and no one will miss the children. It doesn't help that the Academy City's Board of Directors allows this kind of activity.
Academy City is just a testing ground for them to be able to get the god-like power of a Level 6. Everyone within are guinea pigs for many kinds of experiments, at least that's what Therestina Kihara Lifeline said.
The Kihara Family as a whole takes the cake with this trope. Luckily, it's not without exceptions.
Get a Hold of Yourself, Man!: Shirai does this to Uiharu in Episode 23 while the latter suffers through a Heroic BSOD after realizing Therestina had no intentions of helping Haruue, and wasn't really going to help wake the other children up like Kiyama had intended to do.
Godzilla Threshold: Mikoto is so desperate to save Febri's life that she finally involves her friends in the Dark Side of Academy City and asks for the imprisoned Therestina's help.
During the second half of season 2, Mikoto at first contemplates going up against STUDY by herself to find the recipe for the lollipops Febri needs to neutralize the poison in her body. However, after hearing Kongou tell her about her friends being upset she didn't ask them for help, and remembering Touma helping her to stop the Sisters project, Mikoto tells her friends about what she planned to do. Although she was worried about putting them in harms way, they were all too eager to help her out.
Before the final battle of season 2, Mikoto and her friends go to Yomikawa for help, but Anti-Skill has been removed from play due to legal wrangling. They call in Judgement instead. Hundreds of them show up.
Mikoto's so fond of bunnies and frogs it's no wonder she wears shorts under her skirt...
Some of what Uiharu wears may also qualify (though we don't actually see it).
Gory Discretion Shot: When Accelerator is seen fighting a sister onscreen for the first time in Episode 5 of Season 2, he rips one of her legs off. The camera follows the leg as its brought upwards, but it largely tries not to show the lower portion of her body after this scene so as to minimize the gore. As the sister slowly crawls away from him, she reaches for the Gekota pin Mikoto put on her earlier, followed very shortly by him dropping a train car on top of her, and a pool of blood flowing from under the train.
Also another example involving Accelerator. When he cornered a sister in a back alley, he decides to kill her by reversing the direction of her blood flow. If you understand how the human body works, you would know the resultant blood splat which covered the camera was actually doing us a huge favor.
As partially explained in the main entry under Alternate Character Reading, all the Gratuitous English skill names and terms are simply readings assigned to that name/term's kanji. eg. Anti-Skill (kanji reads Guardsmen), Dummy Check, Imagine Breaker (kanji reads Illusion/Fantasy Killer), Judgment (kanji reads Disciplinary Committee), Skill-out, AIM (kanji reads No Awareness).
Also the English terms "Level Zero", "Level One", etc. are used for the super power levels.
Green-Eyed Monster: This was a main motivator for the STUDY group in the second half of season 2. No matter how smart or accomplished they were, in Academy City, espers always got the bigger spotlight. The main leader of their group was noted to be an brilliant scientist, but in a Flash Back showing him talking to a group in an auditorium, there were in fact very few people there. So his group intended to create wholly artificial beings with esper-like powers with the intention of mass producing them and rendering normal espers obsolete.
Guilty Pleasure: In-universe example: Mikoto has a love for childish cute things like the Gekota franchise, but feels incredibly embarrassed about it and tries to hide it because many characters point out how her likings are rather childish.
Ham-to-Ham Combat: Kuroko and Frenda get into a heated argument in Episode 17 of Season 2 as to whether Mugino or Mikoto would win in a fight. It culminates into Frenda grabbing Mugino's breasts, while Kuroko fawns over Mikoto's chest, causing both ladies to smack their respective partners.
Headdesk: When given the idea that Mikoto could contract the stripper lady disease, Kuroko does this
Heart Is an Awesome Power: X-Ray vision may not seem to be that impressive on its own, but when that means Konori Mii can never be surprised by concealed weapons, coupled with her bone-breaking martial-arts skills, makes her more than a formidable match in battle.
Kuroko suffers a minor one in Episode 11 of season 2 when Uiharu and Saten both mention that Mikoto's recent behaviors such as not eating much and sneaking off at night is due to her secretly meeting a boyfriend. The shocked look on her face doesn't change for several scenes, and she ends up putting too many sugar cubes into her drink.
Anti-Skill, Academy City's real police force. Despite the fact that always seem to be 10 seconds too late to help Kuroko arrest criminals, or stop Misaka from using them as a lightning rod, they really are useful at times, and actually have a bit of a cavalry / The Rest Shall Pass moment when they disobey orders and keep Telastina's mook army occupied during the highway duel.
This can also be applied to Touma, who is literally the hero of the ''other' story. Most of the time he will show up just walking in the background, and a few times he encourages Misaka, or points out how stupid she's being, there are occasions when he becomes The Cavalry and saves her from various threats, ranging from exploding balls of aluminum, or just the son of the head of her school.
The true purpose of the "Level Upper" is simply to harness the combined mental power of 10,000 espers as a super-computer substitute; this has the side effects of temporarily increasing the participants' powers (through leaked experiences) and granting the mastermind All Your Powers Combined but later sends the users into a coma.
And of course, the SISTERS' hivemind also makes an appearance here.
Hollywood Hacking: Misaka Mikoto herself. Granted, she's a master of electricity, but bypassing passcard locks, hijacking security robots, erasing herself from security footage, and breaking into a secure facility's network from a phone booth can't be THAT easy, even if you can control electricity. Have to just chalk it up to skill plus Rule of Cool.
Mikoto finds out about a project to clone her... only to discover moments later that it was canceled. She misses the part where it was repurposed for something far worse.
Shinobu attempts to upload emotions to the Misaka Sisters Network, in the hope that they would start to refuse orders, and ultimately be freed from the project. Unfortunately, Last Order blocks said command from being executed, and Shinobu is knocked unconscious and taken away before being able to do anything else.
Shortly after Mikoto destroys the second to last lab involved in the Sisters Project, and finds out that the company behind the last one has declared bankruptcy. She thinks that she's finally set them free and allowed to live as they wish. But the lab managed to outsource the data, and now there are dozens more working on the project. No matter how many Mikoto destroys, there will always be more willing to take up the work.
Mikoto decides to hack Tree Diagram to change the equation and stop the project. Only to find out that the satellite was destroyed weeks ago. This also pre-emptively throws a wrench into her backup plan to destroy the satellite in the hope that the program wouldn't be able to run without it. That obviously is off the table.
In the first episode of the Railgun S anime, Erii gets taken hostage.
Hot-Blooded: Mikoto's battles, especially those in Episode 12 and 24 qualify; In particular the second, her final battle atop a speeding vehicle to save Harumi's comatose students from the clutches of Big Bad Therestina in a Humongous Mecha. When she catches the mecha's Rocket Punch, rips it off the and fires it like a giant missile BACK INTO the villainess to the heart-poundingly Hot-Bloodedbeat of "LEVEL5 -judgelight-", Manly Tearswill be shed. Quite an accomplishment considering the hero in question is a girl.
When Therestina, in her giant robot, is chasing Mikoto, she says that her railgun has a range of 50 meters. Mikoto then tells her that coins are not the only thing she can shoot, and proves it by shooting the robot's own detached claw, which must have weighed at least as much as her, at the robot. It could also count as a Power Limiter.
On a more general note, any time a Level 5 doesn't instantly vaporize their enemy, they were holding back.
I Just Want to Be Special: A large part of Ruiko's (and other minor characters who aren't Espers) angst, as they has absolutely no potential in Esper ability, in a city full of people training to be Espers. Then she, along with many others, gets her hands on the LevelUpper...
I Love This Town: For all the hardships, heartaches and ferocious battles fought throughout the series, Mikoto warmly muses that it was all worth it for the laughter, love and friendship made amongst the way, and quotes this as a summation of her happiness living in Academy City.
Inverse Law of Complexity to Power: The level 5's of the world seem to have very broad powers. Misaka boasts power over electricity, while Accelerator has control over all physical vectors. Lesser espers have abilities like Jufuku Miho's Dummy Check, which simply makes her less noticeable to those around her.
Invisibility: The anime mentions offhand that there are 47 students with the power to turn completely invisible, and that count doesn't include more specific powers like the ability to not be noticed by direct sight.
Ironic Echo: During the Tokiwadai Midsummer Festival, Uiharu berates Saten for eating a beautiful flower made of sugar. Later, Saten berates Uiharu for cutting and eating a beautiful cake.
Irony: In the second season's Breather Episode, while Kuroko is learning an Aesop about how she doesn't have to do everything alone, Mikoto is fighting a one-woman war against the Level 6 Shift Program.
Kid Detective: Our heroines' favourite pastime when together, the main source of their many adventures.
Laser-Guided Amnesia: In the Manga. Done to Uiharu, Saten, and Kuroko by the #5 Level 5, The Queen of Tokiwadai. They've forgotten their friendship with Mikoto.
The Index DVDs contain an 8-part Railgun side story mini novel written by the author of the novels, Kazuma Kamachi and art drawn by Motoi Fuyukawa of the Railgun manga. Wanna have good reasons why you want to read this mini novel? Lets see:
The Railgun cast vacationing in the USA with a Beach Episode too.
The Aztec's flying Fortress versus Xochitl, the Aztec magician who first appeared in Volume 15 of the Index's light novels, flying on her surfboard and teaming up with Mikoto who's is flying. Yes, Mikoto is flying and fighting a flying fortress! Get the Limited Edition DVDs now!
For the Railgun DVDs, the limited editions contains an 8-part Index side story mini novel staring Kaori Kanzaki, written by the author of the novels, Kazuma Kamachi and art drawn by Kiyotaka Haimura of A Certain Magical Index light novels in each DVD. So what's it about?:
Kaori meeting Elves and facing one of Odin's Valkyrie in Europe.
Meaningful Name: Shirai Kuroko's name literally translates as "White-Well Black-Girl", a nice contrast that reflects her outwardly cute but inwardly perverted persona. Mikoto even points this out in Episode 2 before beating her senseless, shouting "I'm gonna fry you as black as your name!!"
Meido: Episode 19 has several, including a rather uncomfortable looking Mikoto and reveals that Acadamy City has a school dedicated to training them.
Metallicar Syndrome: Averted, the fact that Harumi Kiyama drives a very distinct Lamborghini Gallardo helps considerably when they're trying to find her on traffic cameras since they recognize it immediately.
Mission Control: Uiharu puts her computer skills to good use in Episode 3, watching the action through an extensive web of surveillance cameras and giving directions to the other three girls through their earpieces.
Mikoto's side of the Sisters arc. Oh look, Mikoto is playing with some kids! Cut to her clone cleaning up a room filled with another dozen murdered MISAKAs. Isn't it adorable, seeing Mikoto and her clone behaving like real sisters? Then Misaka 9982 gets brutally murdered by Accelerator. The anime is worse than the manga in this regard, since in addition to Mikoto's cute scenes, the plot also cuts to Saten, Uiharu, and Kuroko's Slice of Life antics in the middle of otherwise dramatic episodes.
Actually averted in one specific instance: Episode 5 of Season 2 ends on a very dark cliffhanger, which would jar with the fun and upbeat ending theme. They solved that problem by simply not having an ending theme that episode.
In the Episode 13, when they are transported to a Mountain during a blizzard, Saten hugs Uiharu. Normally it would be just a show of protectiveness, but considering how Uiharu's power is keeping objects' at the same temperature all the time, Saten is simply taking advantage of Uiharu's power not to freeze like the other girls.
Dr. Harumi in Episode 11, when she realizes that the kids were just guinea pigs for Dr. Kihara's brutal experiments.
Misaka in Episode 23, after she realizes that Therestina tricked her into giving the location of the children.
Mikoto again in Episode 6 of Season 2, when she learns that the scientists who used her DNA simply cloned copies of her to die in battle against Accelerator, instead of helping others as they originally led her to believe.
Mikoto gives some advice to Saten telling her that levels aren't important in life yet she is quietly offended by this since Mikoto's a Level 5 while she's a Level 0. Mikoto realizes the consequence of what she said when she learns Saten fell into a coma using Level Upper and the AIM Burst begins broadcasting the collective thoughts of all 10,000 Level Upper users: I Just Want to Be Special.
Mikoto led Therestina Kihara Lifeline straight to Harumi's lab and the Child Errors who are indirectly responsible for the Poltergeist incidents. As luck would have it, she takes after her grandfather just like his son did.
No Communities Were Harmed: Academy City is based on the towns of Tama Center and Tachikawa near Tokyo. The monorail that runs between them appears throughout, and many other locations are clearly recognizable.
No Sell: The first Misaka sister seen fighting Accelerator onscreen in Episode 5 of Season 2 tries an experiment in which she sets off a landmine in an attempt to get through his barrier. While it does blow up, a few moments later he walks through unharmed.
Mikoto attacks him shortly after the first sister battle in Episode 6 of Season 2. Like the sister, virtually all of her attacks have no effect on him, and its likely he would have just killed here right there were it not for her Plot Armor as well as the arrival of multiple sisters intervening and telling him not to attack her which would disrupt the experiment.
Kiyama and Mikoto. Both of them are good people who became cynical due to the fact those they loved were screwed by the corrupt Academy City administration (for Kiyama is was her students, for Mikoto her clone sisters) and both of them could not fight them as they were powerless against the administration. Though as shown in the "sequel" Index, Mikoto eventually grows out of said-bitterness. And thankfully both of them got their Happy Ending.
During the Upper level arc, Ruiko mentions that she can relate to those people using the level upper program to obtain Esper powers, since none of them have any natural ability to do it, just like her. Misaka gets a glimpse of their pain while fighting the AIM Burst creature.
The researchers in charge of the Sisters program are implied to be fairly normal people who simply don't see the Sisters as being human. The analogy used is like sacrificing guinea pigs to cure cancer: You can do it because you don't believe you're committing murder, but rather making a small sacrifice for a greater good. A few of the researchers have better moral compasses, but they're outnumbered and powerless.
It doesn't help the reason why they are trying to make a Level 6 in the first place is so that Aleister Crowley, the ultimate Big Bad of the entire Raildex verse can repeat the process on himself and turn himself into a God.
Offscreen Moment of Awesome: We don't get to see Kongo Mitsuko's rescue of Mikoto from the power armored MAR goon after Therestina knocks her out. But it's subverted in the next episode where we do see her powers and it was awesome!
Oh Crap: Several characters have that look on their face, usually Kuroko or one of the Misaka sisters when fighting Accelerator.
One-Man Army: Several characters attempt to take out various opponents by themselves, such as Shirai and especially Misaka.
Yoshikawa berates her fellow scientists over wasting their time figuring out what type of panties Mikoto wear so they could make the Sister clones wear the same panties when they only need the cheap discount panties she bought for them. Even the Supercomputer Tree Diagram thought their idea was stupid.
On a similar note Chapter 43 of the Railgun manga Lampshades Mikoto as "the only sane Level 5".
Ojou: Most, if not all, of the Tokiwadai students are Ojous.
Mikoto running into Touma, which happens time to time before and during the events of the first and third novels.
Since the Sisters arc is included in the manga, Accelerator also makes an appearance.
Rescue Romance: Subverted. Touma's and Mikoto's first meeting has Touma trying to safely lead her away from another gang and unintentionally pissing her off with repeated references to her being just a brat while talking to the guys. Electricity ensues.
The events of the Level Upper arc in the anime took place two weeks before the 1st Index novel which the latest manga chapters confirms.
In the 12th novel of Index Mikoto greets Uiharu with "You are Uiharu Kazari, right?" as if they had only met once or twice before. Odd considering that they spent the whole summer solving cases together. This was of course before the Railgun manga (and their adventures together) was published. In an omake in the 4th Volume of Railgun this is explained as Mikoto just having forgotten Uiharu's first name. The omake is even called "Matching consistency."
Lampshaded in A Certain Scientific Railgun ch 56.5, in regards to the movie and the Endymion Space Elevator.
Uiharu: The titular Endymion is the world's first space elevator! It has been under construction since 3 years ago without the city revealing what its purpose is! It is a bridge connecting Earth to space! Saten: Oh... Hey, Uiharu. If it's been under construction for three years, then that ridiculously huge tower was already there when this manga began, right? Has it really been here all that time? Uiharu: What are you saying, of course it has. Did Mental Out mess with your memories? Saten: But it's never been drawn in the background before... Uiharu: It just so happened to be outside the panel. Saten: Don't you call this a retco— Uiharu: IT JUST SO HAPPENED! TO BE OUTSIDE!! THE PANEL!!! Saten: O-Okay.
Revision: More details are given about certain people in the novels without changing the plot much like how did Last Order gain human emotions, what lead to Amai's bankruptcy in the first place, the rivalry between Mugino and Mikoto, where Amata's behavior came from and the original creator of Capacity Down.
Roaring Rampage of Revenge: In Episode 7 of season 2, while it takes place largely offscreen, and Misaka takes great stride not to actually kill anyone, she starts attacking labs that are responsible for the Sisters project, vowing to put an end to the apparent atrocities they've caused.
Rubber Face: Kuroko does this to Uiharu in Episode 7 of Railgun S.
Scissors Cuts Rock: During the Sisters Arc, Frenda Seivelun makes the mistake of underestimating Level 5 Electromaster Mikoto by using ceramic, the strongest insulator of electricity, against her. Unfortunately, however, ceramic doesn't take heat very well.
In Railgun, a team of top scientists spending an entire omake chapter speculating on what kind of panties Mikoto wears under her shorts, even sending a special ops team in a (thwarted) attempt to steal them.
In Episode 4 of season two, Misaka spends quite a bit of money to get a label pin of Gekota. After not finding it in the first vending machine, she takes the kids she was playing with to another one, and finally manages to get one, although she immediately realizes she can't wear it openly on her clothing without looking like an immature kid.
Shock and Awe: Mikoto has power over electricity, which she exploits in her signature railgun attack, accelerating a coin to multiple times the speed of sound. She also uses it to materialize a chainsaw whip out of nearby iron particles in Railgun. Whatever else you say about her, she certainly is creative as a Level 5.
Shoo Out the Clowns: Even as fond as the anime is of watching middle schoolers eat and lounge around doing vaguely girly things, all of Mikoto's friends are kicked out of the plot when the Sisters arc gets underway. Only Kuroko really gets to appear and even then her role is almost entirely serious.
A Shout-Out to the 1942 classic Casablanca appears in Kuroko's imagination in Episode 2, with Mikoto playing Humphrey Bogart's Rick and Kuroko as Ingrid Bergman's Ilsa on the mist-filled runway in the finale; right down to Mikoto/Rick lovingly caressing Kuroko/Isla's cheek saying "Here's looking at you, kid!"
In Chapter 25 of the manga, as Mikoto resolves to stop Project: Radio Noise via terrorist attacks, she says "Just who do you think I am?" This is a more-than-likely Shout-Out, as she is attempting to touch the untouchable and break the unbreakable.
In Episode 7 of the second season, the main characters visit a riverbank to help a girl look for a four-leaved clover to give as a present, which harkens back to a key moment in Honey and Clover.
Springtime for Hitler: The Level 6 Shift Experiment was meant from the very beginning to be stopped so that the Misaka Network, the real purpose of the project, could be spread world wide as a part of Aliester Crowley's plans.
Stock Footage: Averted, making it difficult to determine when events are occurring compared to Index.
Misaka kicking the vending machine at the end of Episode 7 is reused from the 10th episode of Index, down to which can comes out. The only difference is that Touma is not in the background.
Stupid Sexy Flanders: During Episode 13, most of the girls are just talking or bickering before posing for photos. Then comes Mii in her swimsuit. All the girls immediately shut up and just stare in awe at her. Hell, even a female snake! This is history in the making, when a trope surpasses the boundaries of species!
Suspiciously Specific Denial: In Episode 16 of season 2, Saten helps Mikoto to make some cookies. In Episode 17, it shows some further details during a Flash Back, and Saten asks if the cookies are for her new mystery boyfriend. Mikoto starts to deny that its for him, and that she's just simply repaying a favor. But she says it in such a cute and clumsy way that Saten can't help but to smirk and pry even more information out about why she's baking those cookies.
Tap on the Head: Mikoto knocks out Shizuri Mugino this way in Episode 10 of season 2, when she uses a doll loaded with a bomb and metal to smack the latter on the side of the head. While it knocked her out long enough for Mikoto to finish destroying the lab, it doesn't keep her down for too long.
Technopath: Mikoto can directly control security computers, cameras, robots and read the circuitry of computers directly as an extension of her electricity powers. Truly a Level 5 indeed.
Tele-Frag: Kuroko's teleportation ability displaces anything in the target area, so she can use it to stick metal needles directly into a person's body...or use plate glass windows to topple a building by slicing all the support beams.
Teleporters and Transporters: Kuroko can teleport herself or anything she comes in contact with as long as it weighs 137kg. The higher concentration required relative to other types of powers prevents her from using it if she can't stay focused.
In the beginning of Episode 11 of Season 2, the scene starts out similarly as it did during Touma's first encounter with Mikoto at the beginning of Index, but taken from her perspective. While several guys are trying to hit on her, she thinks to herself at how people are just walking by, largely because they wouldn't be able to do anything even if they did try due to the sheer amount of guys there. She then mentions that anyone who did try to intervene must be an idiot... just as Touma does exactly that to try and get her out of that seemingly uncomfortable situation.
In Episode 24 of Season 2, STUDY isn't too worried about the few espers attempting to fight them, since they have a huge army of robots at their disposal, have used legalese and red tape to prevent Anti-Skill from interfering, and have an artificial human controlling said robot army. They feel nothing is going to stop them. Until Mikoto shows up anyway...
We finally learn what Uiharu's power is in Episode 22 and it's... "temperature regulation of objects she touches." Consider Uiharu is only Level 1. Now, consider the deeper physical implications of keeping an object's temperature "at will". Put in other words, this is "negation of entropy". This might be what allows her to keep the flowerbed on her head always fresh, and if she had as much creative spirit as Mikoto with electricity, think ofthe possibilities.
There's also the Queen of Tokiwadai's name and face, finally revealed after being in hiding for 20+ volumes in the novels.
The United States is fully aware of Academy City's true technological prowess, including the fact that it has a military.
During the final part of the fight with the AIM Burst.
Happens twice in Episode 24, when Mikoto takes down Therestina's Humongous Mecha with a huge Railgun attack and then when she defeats Therestina herself after Saten destroys Capacity Down.
This happens four times during Episode 24 of Railgun S (Season 2), with "sister's noise" playing during Judgement's battle with the Power Suits, then "eternal reality" playing during more Big Damn Heroes, "future gazer" playing during Shinobu and the Misaka Sisters Big Damn Heroes moment and lastly "LEVEL5 -judgelight-" playing as Misaka intercepts the ICBM.
Theme Tune Cameo: Mikoto singing "only my railgun", the first OP theme in Episode 13 and her violin solo of "Real Force", the second ED theme in Episode 19.
Trailers Always Lie: The first opening of season 2 shows Mikoto fighting ITEM, with a few details wrong. Some are minor (such as wearing her school uniform instead of civilian clothes), while others are much more major—namely, being helped in the fight by Kuroko and Touma.
Trailers Always Spoil: The second opening of season 1 makes it pretty obvious that Therestina is a villain, but at least they don't show her actually fighting the heroes, so it's still a bit ambiguous.
Harumi:You uh... You like him? Mikoto: W-What? Harumi: You know, when a girl behaves all cold towards the guy she likes. It was popular a while back... Tsun... Tsun... Tsundara? No... Tsunjire? Mikoto:Not a chance!
Episode 4 shows us that there are a few of these around. The Undressing Woman and the ability to nullify other abilities are... well the former is shown in the episode and the other is obviously Touma, so those two are confirmed. There are others though.
Goes further than that. Heck, the entire plot of the Railgun anime is based on these. From the Level Upper to Someone's Watching in the OVA.
Villainous Rescue: Mikoto is saved in the arena by Mugino and her partners in Episode 24 of season 2. While she was actually there to destroy the robots that were copying her Meltdowner powers and wasn't too interested in fighting Mikoto, the latter still uses the opportunity to escape with Febri.
STUDY's leaders aren't too worried in Episode 24 of season 2 when some characters try to resist their plans. After all, they have over twenty thousand robots at their disposal, along with an artificial human, Janie controlling them. What could possibly go wrong?
Well-Intentioned Extremist: Kiyama-sensei and her Level Upper plot. A little less extreme than most, since she does have a way to reverse the effects of the Level Upper-induced comas once she's done.
In Episode 4 of season 2, when Misaka runs into one of the sisters for the first time. Then after the credits roll, the two scientists preparing another sister tells her to clean up the mess in a room, which has several dead sisters in it.
In Episode 5 of season two, Misaka learns about the Level 6 Shift, and arrives too late to save her sister Misaka 9982. Accelerator kills her before her eyes.
Chapter 61 of the manga. The "Exterior" Misaki mentioned before turns out to be a cloned portion of her brain grown to gargantuan proportions, designed to allow anyone to use her power. Gensei Kihara hijacks it and uses it to force Mikoto to join the MISAKA Network and undergo the Level 6 Shift.
Chapter 64 confirms that Mikoto can reach Level 6, and if she does, she'll not only die, but take the entire city out with her.
Gensei Kihara: Now, let the experiment begin; Can Misaka-kun reach Level 6?
Wham Shot: Episode 18 of Season 2. Though there are hinting shots beforehand, it is only when we get a below the eyes shot of Nunotaba Shinobu raising her hand that we know that her fate might be resolved in the anime like Harumi's. Episode 20 shows her face in full, showing that she's indeed part of that group of scientists.
Mikoto struggles with this in Episode 6 of season 2 after learning that her clones are just being used as guinea pigs to try and increase Accelerator's power. She tells Shinobu that she's just opposing the program on principal for stealing her DNA map under false pretenses, and doesn't care about the clones. When Accelerator asks her a similar question later, she says the clones are her sisters, and it's her job to protect them.
Later in the same arc, Touma also feels that despite there being many sisters, each one is unique and therefore shouldn't be senselessly killed off. He puts a stop to the project by taking down the Level 5 Accelerator, despite being a Level 0 himself.
You Are Not Alone: Mikoto tries really hard not to let anyone know about her attempts to destroy the Sister's project. While it works on Kuroko, Ruiko, and Uiharu, Touma witnesses enough to figure out what's going on, and when he hears about Mikoto's attempt at a Suicide Mission to die to Accelerator, he intervenes on her, and the Misaka sisters, behalf.
You Leave Him Alone!: Invoked by Saten in the Final Episode as she smashes the device that paralyzed her superpowered friends and left them at the mercy of Therestina, the Big Bad.