Where a person's virginity or absence thereof can be confirmed by some sort of sign or symbol immediately visible on them (that is to say, definitely not requiring a gynecological examination [[note]]In real life, it may be difficult to determine if someone has previously had sexual intercourse even with a gynecological examination -- see [[http://en.wikipedia.org/wiki/Virginity_test here]][[/note]]). This can work through magic, AppliedPhlebotinum, or simply social convention.

Compare DidYouJustHaveSex, NerdsAreVirgins (nerdiness as a Virginity Flag), SexDressed, VirginityMakesYouStupid, VirginPower, VirginInAWhiteDress (clothing as an actual or alleged Virginity Flag), VirginVision (the unjustified form of this). Sometimes, this can be faked with a ConsummationCounterfeit, as when the {{MacGuffin}} is a bloodstain on the sheets. [[noreallife]]


[[folder:Anime and Manga]]
* Everyone in the world of ''{{Manga/Loveless}}'' has kitty ears and a tail that fall off when they lose their virginity. This connection is conveyed through social cues within the story, and exactly what point defines 'the outer border of virginity' is never really defined. For all the sexually charged atmosphere, the lead is a twelve-year-old boy. A grown woman with ears is teased about still having hers, and having already ''lost'' their ears is a sign of some enemy characters' pitiful depravity, but the wider social implications, for example in high school or religious orders, aren't explored, with the most it's addressed being one female high school age character having fake ears that she wears in public.
* ''Manga/{{Hellsing}}'': Played with. When a [[OurVampiresAreDifferent vampire]] fatally drains an opposite-sex human and that human is a virgin, s/he also becomes a vampire. If not, s/he becomes a [[OurGhoulsAreCreepier ghoul]]. While this makes it easy to tell which opposite-sex victims were virgins or not after the fact, most vampires can't tell which humans are virgins or not before they drink. This leads to some vampires who don't want to sire children (like the {{starter villain}}) [[RapeIsASpecialKindOfEvil personally ensuring that their meal is a non-virgin]] before drinking.
* ''Manga/FairyTail'': Larcade's BrownNote ability has no effect on virgins.

[[folder:Fan Fic]]
* [[http://www.fanfiction.net/s/8869552/1/Snowmaiden This]] fan fic implies that everyone in Middle Earth has a VirginityFlag, but only elves can see it. (Overlaps with ''VirginVision'', but the elf claims it is a visible sign in the eyes)

* [[http://www.youtube.com/watch?v=P0Z_OWPe6fU The song]] in ''PodPeople'' has an audio technician wearing a shirt that reads "I'm a Virgin".
** Parodied in the [[http://www.youtube.com/watch?v=7_zrBgzuNjs MST3K bit]] from the same episode, where TV's Frank wears a similar shirt.
* In ''Film/{{Teeth}}'', Dawn wears handmade t-shirt proclaiming abstinence and a purity ring.
* In ''Film/The40YearOldVirgin'', this is first subverted and then played straight. At first, nobody realizes that the main character is a virgin. On the other hand, the daughter of his primary love interest can tell he's a virgin, somehow having gained the ability to spot anyone's virginity flag simply by virtue of spending her high school days with sexed-up teenage boys.

* In the SF shared world of Medea, the resident alien females ''lose a pair of legs'' after having sex.
* In Creator/MadeleineLEngle's ''Literature/ManyWaters'', only "innocents" can ride unicorns. (Otherwise, they disappear.)
* In ''Literature/NineteenEightyFour'', a scarlet sash is the emblem of the Junior Anti-Sex League, which advocates celibacy.
** [[spoiler:Invoked by one of the protagonists, who while wearing it has slept with plenty of men, and simply wears it to ''act'' like the good patriotic Party member]]
* In Creator/MercedesLackey's ''Literature/TalesOfTheFiveHundredKingdoms'', unicorns act as living 'virgin detectors.' Beautiful, beautiful, brainless virgin detectors... This can cause some [[HilarityEnsues issues]] when one does not want their virginity (or gender for that matter) known.
* In ''Literature/TheBible'', after King David's daughter Tamar is raped by her half-brother Amnon, the poor girl tears her garment that was reserved for the King's virgin daughters. Her full brother Absolom immediately realizes what happened when he sees her, [[KnightTemplarBigBrother and it does NOT end well for Amnon]].
* The thranx of the ''Literature/HumanxCommonwealth'' look a lot like giant praying mantids. Unlike [[DeathBySex praying mantids]], male thranx only lose their vestigial wings the first time they have sex.
* ''Literature/SongAtDawn'' It is customary for a bride to wear her hair up after consummating her marriage (i.e. losing her virginity.) [[spoiler: Estela doesn't do it after her marriage because John isn't interested in doing it with her. Instead, she does it after a traumatic experience with a stable hand that she doesn't want to admit to herself was a mistake.]]
* In ''Literature/TheBlackCompany'' short story "Tides Elba", the city's religion the company is stationed in includes that women permanently shave off all the hair below their neckline after losing their virginity. The Black Company being a mercenary outfit full of men with too much time on their hands, this gets discussed at length.
* In ''Literature/TheRedTent'', it's mentioned that among the Canaanite wives that Jacob's sons took, the morning after the wedding night, the mother of the bride would run into the tent and steal the bloodstained blanket [[note]] We now know that neither blood from a broken hymen nor a "tight" sensation are reliable proof of virginity, but this story is set in the Bronze Age. [[/note]], to save just in case Jacob wanted "proof" that the bride was a virgin (and therefore that he had paid a fair price for her). The women of Padan-Aram think this is {{Squick}}, and their [[SexAsRiteOfPassage Ritual of Opening]] is designed to make it so that there won't be a bloodstain so no one will know whether they're virgins or not and judge their worth by that.

[[folder: Live Action TV]]
* In the fourth series of ''Series/{{Misfits}}'', there's a minor character who has the power to make the number of sexual partners a person has had appear on that person's forehead. How the power defines sex for this purpose is not fully specified -- but judging from the effect on characters with known sexual histories, receiving oral sex apparently doesn't count.
* ''Series/AdamRuinsEverything'' [[AvertedTrope averts]] this, with Emily explaining in one episode that female physical virginity is effectively non-existent, as the hymen can be torn any number of ways, and it is not uncommon for it to heal.
* ''Series/BuffyTheVampireSlayer'': in "Teacher's Pet", the mantis demon prefers to mate with virginal human males (and bite their heads off, pursuant to mantid custom). Her targets include a jock who, after rescue, threatens to sue if word gets out.


[[folder:Music Video]]
* [[http://www.albinoblacksheep.com/flash/bunny The fan-made video]] for {{Music/TISM}}’s “Everyone Else Has Had More Sex Than Me” with everyone’s number of either sexual partners or number of sexual escapades written on their shirts. And yep, there’s [[NobodyLovestheBassist one poor fellow]] with a “0” on there. And one with “[[NoodleIncident 3.5]]”.

[[folder:Newspaper Comics]]
* The creator of ComicStrip/{{Dilbert}} can’t exactly announce when the titular character has sex thanks to newspaper censors, but he [[WordOfGod explained to curious fans]] that he’d show it in-universe by [[GettingCrapPastTheRadar drawing Dilbert's tie uncharacteristically limp]].

* ''TabletopGame/{{Starfinder}}'': Ikeshti that fail to mate during their once in a lifetime rutting season transform into large, muscular, and mindless "riveners" that other Ikeshti consider kill-on-sight.

* In ''Theatre/MeAndMyDick'', the virginal Sally's Miss Cooter wears a flower prominently.

* [[http://www.pbfcomics.com/227/ The strip called "Preserves"]] of ''ComicStrip/ThePerryBibleFellowship''.

[[folder: RealLife]]
* Shepherds in some parts of the world traditionally hang burlap bags of colored dust from the necks of their rams during sheep mating season. This makes it obvious which ewes have copulated and which have not, thanks to the dust-marks the bags leave behind on backs and shoulders.