[[quoteright:259:[[WesternAnimation/TheFairlyOddParents http://static.tvtropes.org/pmwiki/pub/images/trixie_375.jpeg]]]]
[[caption-width-right:259:[[GoMadFromTheIsolation TELL ME I'M PRETTY]]!]]

In fiction, a Twitchy Eye is never merely a facial tic -- for some reason, a twitch reveals your state of mind.

The prevailing notion seems to be that an eye twitch denotes insanity, which can apply to a permanently mad person with a permanent tic, or an unstable character whose twitch manifests along with their madness. As such, it usually accompanies a BrokenSmile, and sometimes overlaps with MadEye.

Of course, there are other emotions that give characters a Twitchy Eye, including anything from extreme annoyance, to horror, to caffeine. so much that *twitch twitch* has become a standard [[SqueakyEyes sound effect]] in anime and manga, as well as a standard anime gesture of slowly raising a clenched fist with a twitching eye. Might be the prelude to a VillainousBreakdown or a HeroicBSOD.

Other times it's not a mood, but an action -- [[PinocchioNose such as lying]] -- that will bring about the twitch.

In RealLife, a twitching eyelid ''can'' be sign of emotional instability, but it's usually a sign of tiredness or severe stress.



[[folder:Anime & Manga]]
%%* Miyabi in ''Manga/AiYoriAoshi''.
* In ''Anime/CodeGeass'' when Viletta has been convinced by Shirley to pose in an anime-tight [[FanService bikini]], she gets a full on dose of this. With a little {{Foreshadowing}}:
---> '''Viletta''': [[spoiler:You'll die a thousand deaths for this, Shirley.]]
* Kaname gets this a ''lot'' in the high school parts of ''LightNovel/FullMetalPanic!'', mostly due to Sousuke.
* Happened once to Android 18 in a filler scene of ''Anime/DragonBallZ'', in the Buu Saga, when Master Roshi had a chance to sexually harass her.
* Natasha Zabikov gets one on ''Anime/MobileFighterGGundam'' when [[MaamShock a boy calls her "Old Lady."]]
* [[spoiler:Hiro-chan]] in ''Manga/{{Narutaru}}'' has this shortly before she snaps.
* In ''Manga/{{Naruto}}'', Sasuke does this a few times in part one.
* Yukako in ''Manga/JoJosBizarreAdventureDiamondIsUnbreakable'' has the tendency for her left eye to twitch when she is stressed.
* Kagami does this occasionally in ''Anime/LuckyStar'', when she's trying to deal with Konata.
* Nenene in ''Anime/RODTheTV'', multiple times in furious frustration with her new roommates.
* The otherwise unflappable Xellos got an eye-twitch in ''Anime/{{Slayers}} TRY'' after Filia called him "raw garbage".
* In ''Anime/SonicX'', Knuckles' eye has been seen twitching when he was extremely angry on a few occasions.
* ''Anime/YuGiOh'': {{Bishounen}} Duke Devlin gets a twitchy eye when he's losing to Yami at a game of Dungeon Dice Monsters (Which ''he'' invented). Ouch.
* Max does this in ''Anime/{{Pokemon}}'' after Ash defeats Norman in a Pokémon battle.
* Karina from ''Anime/TigerAndBunny'' gets this when she and the other heroes are asked to try to calm down the mayor's infant son, a telekinetic whose powers activate when he gets frustrated.

[[folder:Comic Books]]
* In ''ComicBook/TheSupermanAdventures'', Superman's eye twitches in "Roughnecks" after ComicBook/JimmyOlsen gives him an ArmorPiercingResponse.
* ComicBook/AtomicRobo does one with his artificial eyelids in "The Savage Sword of Dr Dinosaur", upon running into the eponymous dromaeosaur under the ruins of Science City.

[[folder:Comic Strips]]
* "Your eye twitches like that when you're happy right?" -- from ''ComicStrip/{{Foxtrot}}'', said during an abysmal vacation.
* ''ComicStrip/CalvinAndHobbes'': "Calvin's going to be inside for a week?"

[[folder:Fan Works]]
* Miss Wormwood has one in ''Fanfic/CalvinAndHobbesTheSeries''.
* ''Fanfic/{{HERZ}}'': Asuka's right eye often twitches when she is angry or jealous. Many characters get scared or worried when they see her eye twitching.
-->''"Oh no," said Kensuke, looking really worried.''\\
''"What's wrong? All the readings are fine," said Kaori.''\\
''"Look." Kensuke pointed to the videofeed's enlarged view of Asuka's face. Maya and Kaori leaned forward to take a closer look. Asuka's right eye and brow were definitely starting to twitch.''\\
''"How much longer!? I've got to get out here! That ho is after my husband again!"''
* ''FanFic/QueenOfAllOni'': Jade has one when, after getting curbstomped by Brother Light while trying to take the first tablet, he just gives it to her, saying she could have just asked from the beginning.
** Hak Foo is starting to develop a regular one, whenever someone gets a mask instead of him.
* ''Fanfic/TheElementsOfHarmonyAndTheSaviorOfWorlds'': Rarity has one when Megan tells her she actually was in attendance at the first Grand Galloping Gala.
** Twilight Sparkle has one too, when his idol Star-Swirl the Bearded was referred to as a "nut", by those who knew him personally.
* ''[[FanFic/SonicGenerationsFriendshipIsTimeless Sonic Generations: Friendship is Timeless]]'': When [[WesternAnimation/MyLittlePonyFriendshipIsMagic Spike]] pushes [[VideoGame/SonicGenerations Amy]]'s BerserkButton and leaves Sweet Apple Acres, one of Amy's eyes twitch as she follows the baby dragon.
* ''WebAnimation/SuperMarioBrosZ'': Yoshi when surrounded by {{mooks}} they hadn't seen earlier.

[[folder:Films -- Animation]]
* Sir Ruber of the movie ''WesternAnimation/QuestForCamelot,'' one of the characters with a permanent tic, starts out with a slight TwitchyEye that gets worse and worse throughout the film, until his whole face is practically quivering.
* Ed the hyena from ''Disney/TheLionKing''. He's the least sane from their pack and quite a Cloud Cuckoo Lander.
* ''WesternAnimation/KungFuPanda'':
** Shifu does this when Oogway chooses Po as the Dragon Warrior. This only serves as an early warning signal for the horror that he unleashes on Po.
** [[BigBad Lord Shen]] of ''WesternAnimation/KungFuPanda2'' also has a tendency to develop an eye twitch during his particularly unstable moments.
* ''WesternAnimation/TheIncredibles'': After Kari has been putting up with Jack-Jack throughout the whole of the movie, she manages to freak out the new "babysitter" with one of these.
* Russell being dragged across the Big Bad's window induces a slight eye twitch in the latter in ''WesternAnimation/{{Up}}''.
* A brief example appears in ''Disney/ThePrincessAndTheFrog'': "I was startin' to think that wishin' on stars was for babies and.. ''[[HypocriticalHumor crazy]]'' people.."
* [[EnfanteTerrible Darla Dimple]] from ''WesternAnimation/CatsDontDance'' does this whenever she's straining to keep up her [[TastesLikeDiabetes sickly sweet persona.]]
* ''WesternAnimation/MyLittlePonyEquestriaGirlsLegendOfEverfree'': Gloriosa Daisy's left lower eyelid begins twitching as the problems in Camp Everfree and campers' requests are piling up, another hint of her eroding stat of mind. [[spoiler:Then her other eye twitches too when she reveals herself to Twilight and Sunset in the Crystal Cave.]]

[[folder:Films -- Live-Action]]
* Done dramatically by Gandalf whenever the Ring speaks in ''Film/TheLordOfTheRings''.
* The film version of ''Film/{{Watchmen}}'' does this with Rorschach, though sparingly.
* Quirrell has this in ''Film/HarryPotterAndThePhilosophersStone'' due to his nerves and his generally anxious manner.
* Chief Inspector Charles La Rousse Dreyfus in the ''Franchise/ThePinkPanther'' movies.
* The anchorwoman in the film version of ''Film/VForVendetta'' was said to blink involuntarily when she reported a story she knew was a lie.
* Several characters played by Kitano Takeshi have a twitchy eye, most memorably Nishi in ''Film/HanaBi'', whose eye twitch announces an imminent outburst of sudden violence.
* In ''[[Film/TheKid2000 The Kid]]'', while trying to make a sandwich for his younger self, Bruce Willis's left eye starts to noticeably twitch.
* In ''Film/{{Amelie}}'', Amelie's mother has developed an eye twitch as a sign of shot nerves.
* ''Film/SeedOfChucky'': Glen, who is the genderless offspring between the eponymous killer doll Chucky and his dollified girlfriend Tiffany, always has a notable twitch in his/her eye whenever he's anxious or goes into an AxCrazy episode. He also tends to wet his pants.
* ''Film/JohnnyEnglish'' gets twitchy-eyed whenever Mozambique is mentioned.
* Variation: In the documentary ''Cocaine Cowboys: Reloaded'' the eyes of people in still photographs will suddenly, subtlety move while they are being described as paranoid, high, murderous, or all three at once.

* A man went into a job interview and his eye winked through the whole process. The interviewer said "Look, you are well qualified, but I'm afraid that facial tic will throw off clients." The man replied - "Funny you mention that, because all I have to do is take aspirin and the winking goes away - watch" and the man began dumping out his pockets looking for aspirin. In most of his pockets, however, were condoms. Finally, once a pile of condom packages of every sort piled up on the desk the man found two aspirin, took them, and the wink totally stopped. "Well, that worked," the interviewer admitted, but why do you have all the condoms? We don't want our clients to think you're a womanizer!" "It's quite simple sir," the guy said, "have you ever walked into a drugstore, winking like crazy, and asked for aspirin?"

* In the novel ''Literature/TheGoldenGoblet'', the main character's villainous half-brother has a permanent tic in one eye that makes him look even more sinister.
* ''Literature/{{Discworld}}'':
** Mr Teatime in ''Discworld/{{Hogfather}}'' has an odd twitch whenever his name is mispronounced.
** In ''Discworld/{{Jingo}}'' Lord Vetinari tells Vimes that the reason he doesn't like Captain Carrot in the room when Vimes is being debriefed is that Carrot tends to eye-twitch whenever Vimes tells a [[BlatantLies direct lie]].
--->'''Vetinari:''' I just can't stand to see his poor face twitch all the time.
** In ''Nanny Ogg's Cookbook'' there's a special section on how to talk to Granny Weatherwax. This includes not praising other witches, since her expression will go all solid, apart from a muscle under one eye.
* Prince Andrei Bolkonsky developed facial tics in ''Literature/WarAndPeace,'' but that was probably due to a degenerative disease. The way it's written sounds like his personality (he was a curmudgeony sort) was getting the best of him.
* ''Literature/TheFirstLaw'': Glokta's ruined eye (along with his face, sometimes) tends to begin twitching/blinking uncontrollably when he's nervous, lying, or any other time it's inconvenient to look like he's not utterly composed. He Lampshades it in his thoughts, calling it "treacherous jelly."
* In the novelization of VideoGame/ResidentEvil0, it's mentioned that Birkin's paranoia has caused him to develop a facial tic. Though, it's a slight subversion because it's actually on the corner of his mouth rather than his eye.
* In ''Literature/HarryPotterAndTheChamberOfSecrets'', Lockhart's Valentine's Day antics ''really'' irritate [=McGonagall=].
* In ''Literature/SkinGame'', Nicodemus Archleone begins to suffer from this after only two days being exposed to Harry "FirstPersonSmartass" Dresden.
* ''Literature/StephaniePlum'' starts to suffer from this whenever she's around her family for too long. Going home for dinner or to do laundry is ok, but if she has to move in for a day or two due to issues related to [[BountyHunter her job]], the twitch will get worse and worse.

[[folder:Live-Action TV]]
* ''Series/StarTrekDeepSpaceNine'' featured Jack, a genetically-engineered super-genius who was unstable and borderline paranoid, in two episodes. He was very twitchy.
* Data's EvilTwin Lore in ''Series/StarTrekTheNextGeneration'' had a facial tic when first discovered and activated.
* [[HypercompetentSidekick Captain Darling]] from the fourth season of Series/{{Blackadder}} suffers this, probably down to stress from his wartime enviroment and having to deal with [[GeneralFailure General Melchett]] daily. (Actor Tim [=McInnerny=] had a hard time getting rid of this tic for months after filming ended.)
* On ''Series/MacGyver'', Jack Dalton's left eye always twitches when he lies. It's so well known at the Phoenix Foundation there's a notice in the ladies' room warning about it.
* In ''Series/PushingDaisies'', Ned's eye twitches when he lies.
* Barney in ''Series/HowIMetYourMother'' does this occasionally, most notably when he was trying (and failing) to hide his fear at being on the receiving end of another slap from Marshall on Slapsgiving.
* Peter on ''Series/TheMonkees'' sometimes displays this tic when he is nervous or startled.
-->'''Davy:''' Are you nervous?\\
'''Peter:''' Nervous? Don't be silly! Look at how slowly I'm twitching.
* On ''Series/JustShootMe'', Dennis realizes Nina is trying to keep a secret because of her "I've got an itch to snitch" twitch.
* ''Series/BuffyTheVampireSlayer:'' Xander briefly developed an eye twitch every time anyone mentioned Faith, after they'd had sex together.
* ''Series/BoyMeetsWorld'': Topanga begins to develop a really nasty one during the preparations for her wedding to Cory, which, naturally, are full of wacky hijinks as [[CloudCuckooLander Eric]]... "helps."
* ''Series/TheBigBangTheory'': Sheldon has this (among other tics) when things don't go his way (for instance people interrupting him when he wants to show off his knowledge, or not being able to answer a question to a quizz).
* On ''Series/TheNanny'', [[RichBitch C.C. Babcock]] has it happen whenever she sees Fran Fine and Maxwell Sheffield getting along. It first occurs in season one's "Here Comes the Brood".
-->'''Fran:''' ''[to the Sheffield children]'' Now everybody say goodbye to Daddy...\\
'''C.C.:''' "Daddy"? Did I miss the wedding? ''[her eye twitches]''\\
'''Niles:''' [[LampshadeHanging That twitch becomes you. Is it new?]]
* In ''Series/FamilyMatters'', Carl developed one from dealing with Steve Urkel for so many years. It went away after a therapy session… [[HereWeGoAgain until he saw his bill.]]
* ''Series/ItAintHalfHotMum'': Sergeant Major Williams' eye tends to twitch when ever he is starting to lose his temper. Which is to say, always.
* ''Series/TheGrandTour'': Clarkson and May are supposed to be on a refined [[TitleDrop grand tour]] when Hammond shows up in an obnoxiously loud and unrefined Dodge Hellcat. Whenever he revs it, May develops a twitch in his left eye, signalling a willingness to kill Hammond.
* In the ''Series/AreYouBeingServed'' episode "Gambling Fever" Captain Peacock says when he was hooked on gambling, he had one. It returns in the episode, and at the end of the episode, everyone else has one too.

[[folder:Video Games]]
* Fred Bonaparte in ''VideoGame/{{Psychonauts}}'' has eye twitches... even after he's cured by Raz.
** Boyd seems to have a twitchy eye as well. It's most noticeable when Raz first meets him outside the asylum ruins.
* Happens frequently throughout the ''Franchise/AceAttorney'' series.
** [[spoiler: Kristoph Gavin]] in particular ''is'' this trope, even before his VillainousBreakdown.
** During the "[[LivingLieDetector Perceive]]" tutorial in ''Dual Destinies'', Damien Tenma has this as his "tell".
* Count Veger in ''VideoGame/JakAndDaxter'' gets one when he finds out [[spoiler:the Precursors are ottsels]].
* A twitchy eye is one of the first signs you get that there's something that's not right about [[spoiler: Lisa]] in ''VideoGame/SilentHill1''.
* ''VideoGame/{{Portal 2}}'' gives us a robotic example in Wheatley the Personality Core. He gets damaged early in the game and, from that point until the showdown with the DiscOneFinalBoss, he has a short circuit that randomly causes him to spasm and give off sparks. Since his body is intended to resemble a robotic eyeball inside a frame, the effect is strongly evocative of a twitchy eye in a human. [[spoiler: This actually works as {{Foreshadowing}} that Wheatley is not as sane as he appears to be]].
* In ''VideoGame/MarioStrikersCharged'', this may happen to Luigi if a ball (or more than one ball) gets in the net. Yes, Luigi.
* In ''VideoGame/YandereSimulator'', titular Yandere-chan will begin to have sporadic eye- and head-twitching if her sanity level is too low usually caused by killing a student.

[[folder:Web Comics]]
* The OCD-suffering Hannelore of ''Webcomic/QuestionableContent'' does this when Marten unthinkingly jokes about spreading garbage around her apartment.
* This is supposedly the "tell" in poker of the Patterner in ''Webcomic/ExterminatusNow''. To clarify: the Patterner is an {{Expy}} of Tzeentch, ''TabletopGame/{{Warhammer 40000}}'''s god of [[MagnificentBastard Magnificent]], [[TheChessmaster manipulative]] [[GodIsEvil bastardry]]. When he's shown preparing to deal, he has at least five decks of cards floating around him and held in all four hands. And ''he'' displays he's bluffing when his eye (which is not only all of his head, but floats around separate from his body) twitches.
* ''Webcomic/{{Drive}}'': Nosh does this when he learns that he has to go to Tesskil, whose inhabitants, the [[AlwaysChaoticEvil Tesskans]], lost a war with his own species, the [[GentleGiant Veetans]].
* ''Webcomic/FrankieAndStein'''s male half of the titular duo has an eyetwitch fairly regularly, generally when he has a [[BlatantLies wonderful]] idea. It also appears to be genetic, as it has been seen in his cousin. It may be contagious, as it has also been spotted in Karl's friend Adam, and the female half of the titular duo, Frankie.
* ''Webcomic/{{Homestuck}}'':
** Karkat gets one in the Alterniabound flash game, while demanding that Gamzee stop dancing.
** Jack's had a long two years on the run from the [[RoaringRampageOfRevenge revenge driven Parcel Mistress]], so the sudden appearance of these kids who he's pretty sure he killed already doesn't exactly [[http://www.mspaintadventures.com/?s=6&p=007255 help his mood.]]
** Jane has this while Jake spiels about his conflicted feelings (but is oblivious to hers).
* ''Webcomic/ElGoonishShive'':
** Subtly done when Pandora realises that finding people who can get the kind of powers she wants to give them is [[http://www.egscomics.com/egsnp.php?id=497 harder than she thought]]. And then less subtly when she encounters [[http://www.egscomics.com/egsnp.php?id=501 Ashley]] and [[http://www.egscomics.com/egsnp.php?id=505 Catalina]].
** And by Ashley in [[http://www.egscomics.com/egsnp.php?id=681 the non-canon "Goomanji 2" arc]], when telling herself she has "no irrational grudge" against Catalina.
* Invoked by [[spoiler: Dave Davenport]] in ''{{Webcomic/Narbonic}}'', as part of a threat.
-->'''[[spoiler:Dave Davenport]]:''' Here's the situation: Right now, you need a mad-scientist liaison. I'm a mad scientist. You're going to hire me.\\
'''Dave Conspiracy:''' And what will you do if we refuse?\\
'''[[spoiler:Dave Davenport]]:''' [[TheUnfettered You know, I have no idea.]] ''[twitch]''

[[folder:Web Original]]
* The main character of ''WebVideo/DoctorHorriblesSingAlongBlog'' has this as a CharacterTic. It becomes very pronounced when he's nervous, almost to the point of being a HollywoodTourettes tic. In particular, it tends to occur when he's discussing his ArchEnemy Captain Hammer and when his desire for his LoveInterest, Penny conflicts with his desire to be a {{supervillain}}. In contrast with the typical use of the trope, the tic becomes noticeably less pronounced the stronger he grows in his Dr. Horrible persona.
* When WebVideo/TheNostalgiaCritic reviewed ''Film/SmallSoldiers'' and got to [[NightmareFuel the Gwendy doll scene]], he tried to imagine just how the making of that scene might have played out. A sixteen-year-old Creator/KirstenDunst asks the assistant director just [[WhatDoYouMeanItsForKids why the kids' movie she's starring in]] has a scene where she's BoundAndGagged by creepy, naked, robotic Franchise/{{Barbie}} dolls and then [[DoesThisRemindYouOfAnything taunted in the dark by the villain]], leading him to answer with "look... just do as [[Creator/JoeDante the director]] says, before his other eye starts twitching."

[[folder:Western Animation]]
* ''WesternAnimation/AtomicBetty'': [[BigBad Maximus IQ]] does this after [[spoiler:Galactic Guardian HQ is destroyed]], because Noah and Minimus accidentally do it before he can.
* Guess how Kevin can tell that his friend [[WesternAnimation/Ben10AlienForce Ben]] is lying? It even becomes a minor plot point in one episode.
* Roodaka's eye twitches in ''WesternAnimation/{{Bionicle}} 3: Web of Shadows'' as she expresses her demand for the corpses of the Toa.
* Louise Belcher gets a twitchy eye on ''WesternAnimation/BobsBurgers'', especially when she's angry.
* Detective Piquel ([[ItsPronouncedTroPay "pih-kell"]] [[BerserkButton not "pickle"]]; basically an expy of [[Film/WhoFramedRogerRabbit Det. Valiant]]) develops one after about two days with his manic Toon partner WesternAnimation/{{Bonkers}} (averted with Bonkers' second, younger and more adaptable partner).
%%* Mr [=DeMartino=] from ''WesternAnimation/{{Daria}}''.
* Happens often in ''WesternAnimation/TheFairlyOddParents''.
** In one episode, where various female characters are competing in the annual Miss Dimmsdale-beauty pageant, Timmy gets a twitchy eye when his mom (one of the contestants) shows off that an old swimsuit of hers (in this case, a ''bikini'') still fits.
** Also, Trixie during her SanitySlippage (see the image above) in "Just the Two of Us".
* An episode of ''WesternAnimation/{{Futurama}}'' ends with a shot of Fry doing this after being forced to destroy his robot Lucy-Liu girlfriend, and then watch Bender start making out with the real Ms. Liu. Or at least her head. It's complicated.
* ''WesternAnimation/FanboyAndChumChum'':
** The lunch lady in the episode ''Little Glop of Horrors'' does this when Fanboy insults her glop.
** Not to mention Lenny, who even has an anti-stress twitch cream.
* ''WesternAnimation/MickeyMouse2013'': WesternAnimation/MickeyMouse does this in the short "[[Recap/MickeyMouseS2E2FireEscape Fire Escape]]" when he sees the giant rabbit baby.
* ''WesternAnimation/TheGrimAdventuresOfBillyAndMandy'' uses this a lot. Mostly with Billy's mom and her BrokenSmile.
* Any character in ''WesternAnimation/InvaderZim'' will get twitchy whenever they start feeling crazy, but particularly Gaz and the so-happy-it's-freaky experiment, Nick. For many of the other "normal" humans, it can be interpreted as showing how the stress of living in such a CrapsackWorld has them perpetually near the breaking point, such that they twitch whenever something pushes them just a ''little bit'' further.
* On ''WesternAnimation/JimmyTwoShoes'', Heloise does this after [[CallASmeerpARabbit Cerbee]] and [[TheSlacker Beezy]] ruin her private picnic with Jimmy. She then is paralyzed with rage, breathing deeply and heavily in one spot for hours.
* Lex Luthor in ''WesternAnimation/JusticeLeague'' gets this in "Injustice For All" when the other members of the Injustice Gang stare quietly at him for letting Batman alive instead of killing him.
* Mr. Cat from ''WesternAnimation/{{Kaeloo}}'' does this a lot, usually when somebody around him does something stupid.
* Characters in ''WesternAnimation/TeenTitans'' occasionally do this when annoyed or enraged, such as in "Cyborg the Barbarian" when hero wannabe Krall is upstaged by Cyborg.
* In the second episode of Book 1 of ''WesternAnimation/TheLegendOfKorra'', Korra does this when the radio suddenly turns off, denying her from knowing what happened in the pro-bending match.
* ''WesternAnimation/LooneyTunes'': Wile E. Coyote often gets a twitch during his OhCrap moments.
* ''WesternAnimation/MegasXLR'' has [[{{Cephalothorax}} Magnanimous]] get one after being sucked through a portable black hole. And he lampshades it.
* Used on ''WesternAnimation/MyLittlePonyFriendshipIsMagic'' almost as often as QuiveringEyes, usually when somepony is undergoing SanitySlippage.
** In "[[Recap/MyLittlePonyFriendshipIsMagicS1E10SwarmOfTheCentury Swarm of the Century]]", Twilight Sparkle audibly snaps and develops a twitchy eye when she sees the Parasprites eating Ponyville because of a spell ''she'' cast.
** In "[[Recap/MyLittlePonyFriendshipIsMagicS1E15FeelingPinkieKeen Feeling Pinkie Keen]]", she develops a twitchy eye and [[BurningWithAnger evolves]] [[MemeticMutation into]] [[Anime/{{Pokemon}} Rapidash]] when Pinkie tells her that the Hydra ''wasn't'' the doozy she predicted.
** In "[[Recap/MyLittlePonyFriendshipIsMagicS1E14SuitedForSuccess Suited for Success]]", Rarity earns one of these when she learns the famous fashion designer Hoity Toity is going to be attending her fashion show, and seeing the (tacky and overdone) dresses she made for her friends. She does it again at the end, when [[spoiler:Hoity Toity agrees to sell her fashions at his shop, and proceeds to place a ''big'' order.]]
** Twilight briefly does this while worrying about whether Princess Celestia will approve of her friends in "[[Recap/MyLittlePonyFriendshipIsMagicS1E22ABirdInTheHoof A Bird in the Hoof]]".
** Spike's eye also twitches in "[[Recap/MyLittlePonyFriendshipIsMagicS1E24OwlsWellThatEndsWell Owl's Well That Ends Well]]". ''Friendship Is Magic'' gets a ''lot'' of this.
** In "[[Recap/MyLittlePonyFriendshipIsMagicS1E25PartyOfOne Party of One]]", Pinkie Pie [[BreakTheCutie goes crazy]] when [[HatesBeingAlone none of the other ponies want to come to her party]], and holds a tea party with [[CompanionCube inanimate objects]] complete with CreepyCircusMusic and eye twitches.
** At the [[SeasonFinale Grand Galloping Gala]] in "[[Recap/MyLittlePonyFriendshipIsMagicS1E26TheBestNightEver The Best Night Ever]]", [[spoiler:Fluttershy]] develops a TwitchyEye, EvilLaughter, and DramaticThunder when [[spoiler:none of Canterlot's creatures want to be her friends.]]
** In "[[Recap/MyLittlePonyFriendshipIsMagicS1E21OverABarrel Over a Barrell]]", When the Mane Six reveal that a tribe of buffalo has dragonnapped Spike and [[ItMakesSenseInContext treenapped]] a tree Applejack was bringing to her cousin for his town's orchard, Applejack's cousin Braeburn's eye begins to visibly twitch in shock.
** Twilight does this ''repeatedly'' after [[SanitySlippage going nuts]] in "[[Recap/MyLittlePonyFriendshipIsMagicS2E3LessonZero Lesson Zero]]".
** Insane Rarity has this again in Sweetie Belle's BadFuture dream from "[[Recap/MyLittlePonyFriendshipIsMagicS4E20ForWhomTheSweetieBelleToils For Whom the Sweetie Belle Toils]]".
** In ''[[Recap/MyLittlePonyFriendshipIsMagicS4E23InspirationManifestation Inspiration Manifestation]]'', as more of the tome's power takes hold, Rarity begins doing this.
** "[[Recap/MyLittlePonyFriendshipIsMagicS6E10TheSaddleRowReview The Saddle Row Review]]", after recaping all the problems they're having, and portentously asking "Am I forgetting anything?", Rarity gets a twitchy upon the raccoon family crossing the room.
* In ''WesternAnimation/ThePenguinsOfMadagascar'' Skipper gets this when trying (and failing) to stop a [[ItMakesSenseInContext plumber]] from exposing them. That's not exactly what causes the twitchy eye, it's the fact that he thinks just like Skipper.
-->[[IronicEcho (repeated line)]]There's only one way to do the job, my way. The right way.
** Skipper even [[LampshadeHanging lampshades]] it.
-->Does this eye look weird to anyone.
* ''WesternAnimation/ThePowerpuffGirls'':
** Buttercup does this in "Nuthin' Special" in a scene where her sisters keep matching her in showing off special powers.
** Buttercup and Bubbles do this at one point in "The Powerpuff Girls Rule!"
** Buttercup gets one in "Sun Scream" whilst lying down on a bed.
* Captain Murphy of ''WesternAnimation/{{Sealab 2021}}'' has this moment when his finds out his Happy Cake oven has gone missing.
* In a ''[[WesternAnimation/TheSimpsons Simpsons]]'' parody of ''Theatre/{{Amadeus}}'', Lisa, as Salieri, does this when the emperor declares all music other than [[AnachronismStew Beethoven]] obsolete, making her schemes against Mozart all for nothing. She then hails a coach for the nearest asylum and rides off, [[LaughingMad cackling hysterically]].
** If you pay careful attention, you can see Ned Flanders twitch an eye during "Hurricane Ned" after the rebuilt house falls down, just before he puts his glasses back on and [[FreakOut completely loses his shit]].
** Yet another Simpsons example involves Moe the Bartender. When he attempts to remake his bar into a family restaurant and himself into a more family-friendly image, the customers--as per usual for [[DysfunctionJunction Springfield]]--quickly annoy him to the breaking point. What sets him off on a [[GoshDangItToHeck G-Rated]] ClusterFBomb barrage is a child complaining, during Moe's twitch of anger, that her soda was too cold.
** "New Kids in the Blecch" has L.T. Smash do it when commandeering a navel ship to blow up the ''Magazine/{{MAD}}'' headquarters.
* Tweek of ''WesternAnimation/SouthPark'', in addition to his overall jitteriness, has a perpetually twitchy eye.
* ''WesternAnimation/SpongebobSquarepants'' does this when Patrick meets him in his house. "I'VE BEEN WAITING FOR YOU PATRICK!"
* ''WesternAnimation/TheTick'' has the character of Big Shot, a NoCelebritiesWereHarmed version of ''ThePunisher''. He gets all eye-twitchy whenever he shoots, fights, or even says the word "gun". This is indicative of some pretty deep-seated mental trauma, as he is prone to collapsing into uncontrollable sobbing while screaming "Why didn't you love me, Mommy?!"
* Megatron of ''WesternAnimation/TransformersAnimated'' no doubt appreciates Lugnut's unswerving loyalty, especially in comparison with [[TheStarscream Starscream's]] constant backstabbing, but once in a while gets a twitching ''optical sensor'' when [[SycophanticServant Lugnut]] overdoes the fawning adulation. "Just...go." * twitch twitch* [[http://youtu.be/jTduxJxuGak Observe.]]
* Earlier in the franchise, [[TheDragon Galvatron]] starts to exhibit a serious twitch towards the end of the fight with [[TheChosenOne Hot Rod]] in ''WesternAnimation/TransformersTheMovie''. Justified, given that his day has gone quite south, he's had his psychic leash yanked for much of the movie, and he later has a reputation for being, well, just plain nuts.
* Miss Achmetha from ''WesternAnimation/VeggieTales'' has this as a character trait, and comes up pretty much every time she's used.
** A subtle one in ''Duke and the Great Pie War'' done by Duke (played by Larry):
--->'''Duke:''' I'll take the king's brother on! [[TemptingFate I'll even take on]] [[BigBad Otis the Elevated]]! ...By the way, who is the king's brother?\\
'''Madame Blueberry's character:''' Otis the Elevated.\\
'''Duke:''' *StunnedSilence, eye twitch right before cut to black*
* Brock Samson of ''WesternAnimation/TheVentureBrothers'' has a very twitchy eye that manifests when he enters one of his {{Unstoppable Rage}}s. This happens especially when killing people who have threatened his [[TrueCompanions Family]]...or [[spoiler:[[CarFu run him over with a truck]], while he's returning the favor.]]
** When he appears in VideoGame/PokerNight2, this is one of his "tells" to indicate he has a bad hand.
* WesternAnimation/WelcomeToTheWayne: happens to Julia's right eye halfway into "Like a Happy, Happy Bird" after witnessing Wayne Entities and before EyeRubbing that causes HeteroChromia (left blue right green)

[[folder:Real Life]]
* [[http://en.wikipedia.org/wiki/Opsoclonus "Opsoclonus"]] is a term that just broadly refers to any kind of uncontrolled eye movement. A ''lateral'' twitch is specifically called [[http://en.wikipedia.org/wiki/Physiologic_nystagmus "Nystagmus"]] and can be a sign of an underlying neurological problem if it persists.
* Can also be a physical tic / compulsion for people with anxiety, OCD and/or Tourettes.