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- The Lone Ranger, in some ways a precursor to Vash, used silver bullets as a symbol of his pledge never to take human life.
- In George Carlin's routine on The Ten Commandments, he ends with this one and claims that because more people have been killed in the name of God than for any other reason, it really comes down to who's doing the killing and who's getting killed. He decides it should be renamed "Thou shalt try really hard not to kill anyone, unless they pray to a different invisible man from the one you pray to."
- GURPS has the Pacifist disadvantage, which comes in several flavors, one of which is Cannot Kill. Characters with the "Cannot Kill" disadvantage can start fights and use any tactics they like, but they cannot kill, or be responsible for a death, or leave a wounded enemy to die. They also cannot stand by while their teammates administer the Coup de Grâce. If they do, they angst about it for days and are effectively rendered useless to the team.
- Some Superhero RPGs would invoke rules against killing. Two notable examples were Marvel Super Heroes and DC Heroes, which would eliminate all Karma/Hero Points (a combination of experience points, and self-boosting reserves for various tasks) and keep you from accumulating more for the rest of the adventure (usually one night of gaming). In DC heroes, this punishment came from using lethal force at all.
- Dungeons & Dragons has the Book of Exalted Deeds, which contains the feat "Vow of Peace". It grants benefits as long as you don't inflict lethal damage, allow an ally to finish off a defeated opponent, or cause similar harm to a creature. It takes this trope to extremes; accidentally swallowing a gnat in your drinking water will cause you to lose the benefits of the feat, in fact the feat specifically references paladins drinking their water through a strainer. It doesn't really make you a pacifist, technical or actual, though; you can still fight all you want, as long as you never inflict lethal damage.
- "Code vs. Killing" is one of the most commonly seen Psychological Limitations in Champions, usually bought as "total commitment" (i.e. the character can't bring him- or herself to kill at all and won't stand idly by while others do it either). Normal people are already assumed to be "reluctant to kill" by default (being Ax-Crazy would be its own different Limitation); the code, if taken, is intended to go beyond well beyond that to proper comic book levels. Of course, being a Limitation that you get points for, it's also supposed to cause your character trouble from time to time.
- In the Old World of Darkness, Lifesaver is a 3 point flaw that makes you unwilling to take life. Pacifist is a 5 point flaw and is taken literally - the character can do no physical harm to others.
- Defied in Sentinels of the Multiverse. In fact, the resident Batman Expy has a Flavor Text quote warning against the perils of mercy that originally comes from one of the Caped Crusader's villains. Taken in context, this appears to be a proportionate response thing, since most of the game's villains are potentially apocalyptic threats and and there are examples in the wider lore of heroes limiting themselves (the example given is that everyone's happy to have Fanatic on their side against villains, but nobody's in a hurry to let her deal with a bank robbery).
- In Sluggy Freelance, Torg made Oasis swear one of these vows. She sorta forgets it for a while and becomes an All Crimes Are Equal vigilante. When she remembers, she cries, "I've broken my promise! There can be no wedding! Why does love bring me nothing but pain?" Ironically, Torg himself doesn't really subscribe to this philosophy, as he was perfectly willing to go in guns blazing and swords swinging during "The Stormbreaker Saga" and "Dangerous Days" arcs.
- In The Adventures of Dr. McNinja Mongo the superninja has learned the preciousness of life. And also that fire bad.
- Demon Fist
Duncan: Your crew fought off all my men without killing any of them. Criminals would not have wasted the effort not to kill their attackers. I can't in good conscience take you all in simply for defending yourselves.
- The Demon Fist almost never kills humans or peaceful demons, even (especially!) Mooks.
- Neither does the Hookshot crew. This pays off for them later.
- Decoy Octopus of The Last Days Of Fox Hound passes The Sorrow's test because he has never killed anyone and thus has no one to face. The Sorrow is very surprised and Octopus just shrugs, claiming he's more suited for espionage than fighting.
- Schlock Mercenary: Petey avoids killing if at all possible, and at one point refuses to let a spy go back home because she'll just get needlessly mind-ripped. After the oafans give everyone immortality, he starts going to truly absurd lengths to avoid killing, like teraporting entire fleets that are in the middle of a massive fight. As he says, he can't be sure these people will still be his enemies in hundreds or thousands of years, so killing anyone is like killing future allies.
- This trope was actively enforced in the Global Guardians PBEM Universe. Player characters who were casual killers were absolutely not welcome, and those that became it later were booted from the game. Accidents still happened, but for the most part the idea the various campaigns operated under was that real heroes didn't kill criminals. The single exception was the Big Easy campaign, but as that campaign was based on The Dark Age of Comic Books, it got a pass.
- Shortly after 9/11, The Onion reported that God held a press conference to remind everyone exactly what He meant by "Thou Shalt Not Kill."
- Averted with extreme prejudice in the Whateley Universe. It's a sad commentary on a superheroic 'verse when the person best known for having a code against killing is a supervillain (Mimeo, a very high-end Power Mimic, so it is not just out of good intentions - he wants to be able to keep getting the power-up from fighting superheroes so he can go after his real targets, meaning that killing his 'donors' would be counterproductive; also, he grew up around mobsters, and was disturbed by the psychological effects becoming a 'made man' had on people he knew).
- All but two of the members of Team Kimba (who are high school students) have been responsible for multiple deaths, and Jade in particular has a body count around 100 - most of them over Christmas vacation in their freshman year.
- It has been mentioned that in addition to several deadly encounters with Nazi spies during WWII, a number of villains have died fighting Lady Astarte due to accidents; like most superheroes, she doesn't choose to kill (and it is a major Character Development moment that she would actually try to kill the Ax-Crazy Blood Knight Deathlist during his attack on the school), but supervillainy is a dangerous field at the best of times, and even in cases where she could do something, she sees herself as being under no obligation to save them from their own mistakes if it would put others at risk.
- In addition, many supposed superheroes and costumed vigilantes show little compunction about killing. The Dark Avenger and the Lamplighter are two of the better known examples, but far from the only ones. This isn't even considering 'heroes' like Jack Rabbit or Iron Mike, who are basically thugs and thieves pretending to be heroes.
- Heroes Save the World: Austin Smith is trying to adhere to this as much as he can. It's disquieting for him when he learns that he has the greatest potential for mass destruction out of the Children located thus far.
- The Red Panda and Flying Squirrel of Red Panda Adventures go out of their way to avoid using lethal force. Though the Red Panda owns a katana, for example, he won't take it into battle so as to not even have the option. They fight with fists, gadgets, and hypnosis. They aren't as firmly wedded to it as other superheroes with a "no killing" code, however. They can and will employ lethal force if the situation calls for it. Fighting non-living foes such as Professor Zombie's undead minions naturally falls into this, but the pair have also been willing to kill if the entire city, or even world is at stake, such as killing the Nazi Ubermensch, Tevas, to keep him away from the Normandy invasion. The one thing that will make either the Red Panda and Flying Squirrel outright abandon this edict is if a villain seems to have killed one or the other. A developing Villain Team-Up decides against killing the Squirrel to get to the Red Panda precisely because they know it will.