Big Damn Heroes: Couple times. The Losers for the children and later Aisha for the Losers.
In the finale of the comic, Pooch shows up to save the team from the oil rig, although only Jensen makes it.
Bittersweet Ending: The Losers stop Max's plan to start World War 3 and insure global American dominace, but Aisha betrays the team and kills Clay by dropping a WP grenade next to him. Cougar is mortally wounded trying to escape, and ends up detonating a nuke to destroy Max and his country. Aisha's last appearance has her fighting 100 of Max's goons with no indication she got far enough away from the blast in time. Jensen and Pooch manage to make a clean getaway and it's made clear nobody who wants them dead knows they survived.
Brick Joke: In the movie (see Real Men Wear Pink below). After the first appearance of the Petunias tee, Jensen is shown doing research on their opponents, the Marigolds - which turns out to be comprised entirely of Huge Schoolgirls. Later during the credits, we see the actual match.
Another: while hiding out in Bolivia, Cougar and Jensen go to work in a factory making "Girls of America" dolls. Later, after Aisha shoots Jensen, the team breaks into a pharmacy to patch him up - and the first thing we see is a whole shelf of those dolls.
But for Me, It Was Tuesday: In the movie, Max does not remember setting up anyone and has no idea who Clay and his team are. Wade has to remind him about Bolivia for Max to even get a vague recollection. Max claims he can't be bothered to remember every time he has someone betrayed and/or killed.
California Doubling: A rare double subversion. The entire film was shot in Puerto Rico, including a segment set in...the Port of Los Angeles.
Car Fu: Twice in the Montserrat part of the book. The first time Clay takes out a paratrooper in mid-air. The second time they hijack a tilt-a-rotor by driving into it...and on top of the unsuspecting welcoming committee.
Even Bad Men Love Their Extended Families: Movie Wade is not pleased with being ordered to kill some random team he assembled to take down the Losers for this reason. One of the team members is Wade's brother-in-law, but he insists this isn't a dealbreaker.
Even Evil Has Standards: The Losers aren't evil, but they do kill for a living, and even they are completely unwilling to allow 25 children to be killed while trying to take out a target.
Female Gaze: The women on the floor Jensen's elevator accidentally stops on while his, ahem, dangly parts are exposed. They can't be blamed, though: Chris Evans is Mr. Fanservice to the umpteenth degree.
Jensen: You ladies liking the angle of the dangle?
Jerkass: What Max is to literally everyone around him.
Kick the Dog: How else do you explain blowing up a helicopter full of children?
Of course, the movie is one long continuous Kick the Dog moment for Max.
Laser-Guided Karma: Camera-guided, anyway. After Roque captures Pooch and cuts off one of his fingers, he prepares to blow his head off. Then Jensen, remote-piloting a bomb-disposal drone, shoots Roque with the drone's shotgun.
Jensen:Ohhhhh...shit. She's got a gun and...it's pointed at my dick. CLAY, IT'S POINTED AT MY DICK!! Pooch: Would you rather it was pointed at your face? Jensen: I KNOW IT MAKES NO SENSE, BUT YES! [Aisha aims at his face] Pooch: Better? Jensen: Not...really.
Mythology Gag: Max in the Movie sometimes wears a white glove or a black glove on his right hand depending on the scene. This is because in the comics, Max is actually two people who are twins.
Also Max going from plunging unto the water to being on a bus within 5 minutes, is also possibly invoking that.
There is a possible Shout-Out to the original in the comics; Clay shares a surname with one of the original Losers, and he mentions that his grandfather was in World War II. Pooch is named after the dog kept by Gunner and Sarge of the original squad, as well.
Never Found the Body: Lampshaded when Roque turns up alive and well unexpectedly and belts the crap out of Clay, telling him to always check for a body. Clay takes it to heart; after a later episode wherein the hunt for Roque winds up sinking a couple of ships, someone asks if Clay thinks Roque is dead. Clay says he won't believe it until he sees a body.
In the final scene of the comic, Pooch and Jensen are talking about how it's almost impossible Aisha could've killed a hundred man and escaped New Jerusalem before the nuke went off. But they can't be totally sure, because it's Aisha they're talking about.
Jensen: Did you know that cats can make one thousand different sounds and dogs can only make ten? Cats, man. Not to be trusted.
Pooch: You know what? Do me a favor—NEVER say that again.
Nonverbal Miscommunication: When on top of a tall building, villain nods to his agent, who then throws a businessman down. Villain then "complains" that it wasn't that kind of nod and he just wanted the agent to beat the victim.
Sixth Ranger Traitor: Aisha is suspected of being one initially, but then it turns out Roque is really The Starscream and Aisha goes all Big Damn Heroes on them. In the book the same thing happens at the start, but then right at the series conclusion she reveals that she's been one all along, and now they've served their purpose she doesn't need to save them.
Small Girl, Big Gun: Aisha bailing out the Losers with an RPG. More like Regular Girl HUGE Gun, really.
War for Fun and Profit: One of the ways that Max gets his funding for his grand plan is P.A.M. (Policy Analysis Market) a special program that reads changes in the stock markets as a way of predicting terrorist attacks and also allows investors to earn huge profits by betting on the probability of said attacks. Max also runs a special outfit called P.2.O.G. (Proactive Preemptive Operations Group) whose objective is to provoke terrorism. You do the math.