- Yosuke Hanamura from Persona 4. He even says word for word "I'm cool playing second banana."
- Luigi from the Super Mario Bros. games. Brawl in the Family got a joke out it.
- Art Carney was Second Banana to Jackie Gleason in The Honeymooners.
- In The Andy Griffith Show, and later in Mayberry RFD, Don Knotts was the second banana.
- Neil Patrick Harris's Barney Stinson is the second banana on How I Met Your Mother.
- Vivian Vance was usually Lucille Ball's second banana in I Love Lucy and The Lucy Desi Comedy Hour.
- There was a character (a mouse) actually named Second Banana in some Warner Brothers cartoons, and in some Gold Key comics based on them. He was the sidekick of Merlin the Magic Mouse, a vaudeville-style stage magician.