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He tampered in God's domain.
Bride of the Monster

Yeah, but your scientists were so preoccupied with whether or not they could, they didn't stop to think if they should.
Dr. Ian Malcolm, Jurassic Park

Writers are not scientists. Whether it is because they perceive science as cold and emotionless, or because they just disliked science and embraced literature after failing math in high school, luddism is an all too common failing of writers in search of plot. The typical theme is that some sort of advanced scientific research has Gone Horribly Wrong, creating a monster, causing an impending natural disaster and/or a massive government cover-up. The heroes typically discover the side-effects of the research and investigate, discover what's going on, and try to stop it.

The antagonist (almost always either corporate or military/government scientists — and not hot) refuses to believe that his work could be so badly flawed, immoral, or simply doesn't care about who gets hurt by it, insisting that the research is For Science! They will generally use their influence with the government to make life difficult for the heroes, try to have them arrested and otherwise silenced, often leading to a shoot-out, jail break, or Chase Scene.

In the end, the scientist will be destroyed by his own creation, the heroes will be proven right, and through their efforts the world will be saved from the horror of science. Sometimes the theme is softened by the presence of The Professor among the heroes who represents a more reasonable take on the science involved.

This can often come off as a bit hypocritical, particularly when dealing with speculative fiction, as you get an Anvilicious message of "everything we have so far is good, but we should stop now."

Nearly every Robot War story is based off of this (except the ones where everything was all right, until humanity screwed it up by being jerks to the nice robots). There are a few popular current fields as well, like cloning, genetic engineering, and surveillance.

For obvious reasons, this is played down in series starring a Science Hero, heroic android or Robot Buddy such as in some anime. It's more likely there will be a (still obvious) distinction between good and bad scientists. This is usually played up if the heroes are Phlebotinum Rebels, though.

Note that not every work with a Mad Scientist or a threat borne of science falls under this; it's only the case where Messing With Things You Ought Not To is blamed for the problems.

The trope rarely makes a distinction between pure science and applied technology.

Frequently overlaps with Green Aesop.

Related tropes include the Mad Scientist, The Evil Army, Government Conspiracy, Corrupt Corporate Executive, Government As Villain, Mr Exposition, Technical Pacifist, and Well Intentioned Extremist. The protagonist is often assisted by an Anti Hero who used to work for the Mad Scientist, and frequently has to deal with a Pointy Haired Boss. See also Science Is Wrong. Polar opposite of most stories with a Campbellian Hero.

See also the Scale Of Scientific Sins.

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Deus Exit MachinaPower At A PriceAesoptinum
Death By ChildbirthUndead Horse TropeDeath By Origin Story
Popular Is DumbAnti-IntellectualismStraw Vulcan
Scheherezade GambitNarrative DevicesScience Is Wrong