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Polar Opposite Twins
aka: Different As Night And Day

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And this is before they were redesigned.
"When me and my sister were little, we had two older twin sisters who would take care of us. We always called one The Angel and one The Devil, because one was really nice and the other was a complete bitch."

A Sub-Trope of the Odd Couple wherein the odd couple are twins, usually identical brothers or sisters (or cousins, as long as they're still using the "identical appearance, contrasting personalities" motif).

Basically, Sibling Yin-Yang for twins, as there is an extra bit of irony when people born at the same time (and who usually look the same as well) are so different. They'll usually embrace using Identical Twin ID Tags to help the differentiation.

Sometimes overlaps with Cain and Abel, in which case you might expect Fearful Symmetry. Also frequently overlaps with Foolish Sibling, Responsible Sibling and Red Oni, Blue Oni. Compare Evil Twin. The extreme opposite is Single-Minded Twins or Creepy Twins.


Examples:

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    Anime & Manga 
  • Tsubasa and Arisa from Arisa. Arisa is loved by everyone at her school, has good grades, and is the Class Representative. Tsubasa is nicknamed "the Demon Princess" and is constantly getting into fights and has lousy grades. As it turns out, Arisa is very much a Broken Ace.
  • In Aruosumente, the twins Lante and Dante. Lante is cheerful, outgoing, chatty, frilly, and can't fight to save his life. Dante, on the other hand, barely talks at all, keeps to himself, cares only about Lante, and is the best fighter in the kingdom, to the point of being called a beast. Interestingly, it's Lante who may be a schemer and capable liar, while Dante is very straightforward.
  • Black Clover: The House of Faust had twin sons, with Morgen being the picture of a good man, beloved by many and having joined the Magic Knights to save others while Nacht was a delinquent disapproved of by others who used his magic only for himself. Morgen's name even means "morning" in German while Nacht means "night" in German.
  • Bleach: The sweet House Wife-in-trainingnote  Yuzu and the rough Tomboy Karin.
  • Blue Exorcist is essentially about a pair of these, as both Okumura brothers are very different: Yukio is a stoic Badass Bookworm Teen Genius, kind at heart but thoroughly steeped in moral grays, who subverts Half-Human Hybrid due to being sickly when young, while Rin is a Hot-Blooded Book Dumb Almighty Janitor who is the inheritor of Satan's power and holds himself to strict principles for fear his demonic heritage will drive him off the deep end. They fell into this when younger as well, with Yukio being a Shrinking Violet Cute Bookworm (and still has shades of both still), while Rin was a Pint-Sized Powerhouse loner.
  • Digimon Frontier: Kouji and Koichi. Kouji is arrogant and kind of a jerk at first, working by himself because he thinks the others will only drag him down. As time goes on, he shows a much kinder side. His Digimon forms are wolf-based and his element is light. Meanwhile, his twin is similar in appearance but far more soft-spoken and willing to be in the group once he's de-brainwashed. And, his Digimon forms are lion-based and his element is darkness (Dark Is Not Evil).
  • D.N.Angel: Riku and Risa even come out and say that their personalities are completely different. Risa is very girly and rather shallow (most of the time), while Riku is tomboy-ish and more down-to-earth.
  • Eyeshield 21: The Kongo twins Unsui and Agon. Unsui has a shaved head like a monk (it's sort of a theme at their school) is intensely hard-working, diligent, cautious, a very good athlete and basically a decent guy. But Agon (with a distinctive dreadlock hairstyle) is the series' Jerkass, a Jerk Jock to the extreme (including the womanizing, which apparently started in middle school) who gets away with being a sociopathic thug, slacker, and general all-around asshole because he is a talent seen only once in a hundred years. It's emphasized by the fact that 'A' and 'Un' represent "beginning and end" aka birth and death.
  • Fushigi Yuugi gives us easygoing, kind, polite Amiboshi contrasted to Hot-Blooded Jerkass Suboshi. Heck, even their weapons are different: Amiboshi has a flute which he can use for Mind Control on his opponents (for good or evil purposes), and Suboshi prefers to just smash through everything with a Killer Yo-Yo. Even their clothes reflect just how opposite they are: Amiboshi wears a blue tunic, and Suboshi wears an orange one. (At least in the series; in promo artwork, they are very frequently dressed exactly alike.)
  • Toddlers Takuma and Kazuma Mamizuka from Gakuen Babysitters. Takuma is an outgoing Perpetual Smiler, while Kazuma is shy and very Prone to Tears.
  • Although the latter being conceived in a less-than-natural fashion, Amata Sora and Kagura Demuri from Aquarion Evol fit this to a T. Kagura is a hotblooded warrior from the enemy land raised to be a savage killing machine, while Amata is an insecure, shy and gentle guy with issues regarding his mother's apparent abandonment when he was a kid. Them being opposites of each other is actually a plot point.
  • Glass Fleet's Vetti and Cleo exemplify this trope. Though, their personalities are passed off as them having been raised separately, with the younger one subjected to mental and sexual abuse, giving him his screwed-up personality.
  • Hekikai No Aion: The twin sisters Shizuki and Yuzuki. Shizuki is a Manipulative Bitch and a sick girl, on the other hand, Yuzuki is a Ditz and a Dumb Muscle.
  • Hetalia: Axis Powers: the Boisterous Bruiser America and the Shrinking Violet Canada are only implied to be twins, but they fit in fairly well. The show also has sweet, softhearted Italy Veneziano and his brother, the temperamental, hotheaded Italy Romano.
  • High School Star Musical has two pairs - Nayuki's younger sisters, Tsumugi and Yuki, are more typical and aren't major characters. But separated twins Tsubasa and Itsuki - who aren't even known to be related - have a large part of their conflict revolve around how they've developed into this since being separated.
  • Ukon and Sakon Futatsuki from i tell c are twins, and are as different as night and day. Ukon is cool and unflappable while Sakon is hyper-enthusiastic and prone to overreactions. Their hair colors, skin tone and uniform colours reflect their opposite personalities.
  • I'm Gonna Be an Angel!: Noelle (the light) and Silky (the darkness) as described by Mikael. Mikael and Raphael might also count.
  • The Fubuki brothers in Inazuma Eleven. Shirou is a gentle, calm, and timid defender, while Atsuya is a Hot-Blooded and rude forward. Coincidentally, Shirou's hair is bluish-gray and Atsuya's hair is pink.
  • Is Kichijoji the Only Place to Live? has Miyako and Tomiko. Miyako is loud, cheerful, and extroverted while Tomiko is a quiet introvert who prefers books to partying. They do share some things in common though such as their love of heavy metal music.
  • Kiss x Sis: We have Emotional Red Oni Ako and Soft-Spoken Blue Oni Riko.
  • Lucky Star: Kagami and Tsukasa Hiiragi; the former is a smart, no-nonsense Tsundere while the latter is sweet but also ditzy and Book Dumb. When asked about this by Konata, they point out that they are fraternal twins.
  • In Miracle Girls, Toni is an outgoing and athletic tomboy while Mika is feminine, studious, intellectual, and bad at sports.
  • Gundam SEED has Kira and Cagalli. The former is quiet, polite, and kind to a fault. The latter is brash, flippant, and has quite a temper. Also one's a boy and one's a girl. And one is genetically engineered to be physically "perfect", while the other...has superior physical abilities without any genetic alteration.
  • Mars (1996): Rei was always very active and prone to getting himself into trouble while Sei was quiet, artistic, and something of a crybaby. And then the real reason it turned out they were opposites was that while Rei had it in him to be well-adjusted, Sei was actually a sociopath who placed no value in human life aside from him and Rei.
  • Mischievous Twins: The Tales of St. Clare's: While both Pat and Isabel are Academic Athletes, Patricia is a Hot-Blooded, mouthy tomboy, while Isabel is a demure, by-the-book Shrinking Violet.
  • Monster: Anna and Johan Liebert. Anna's a nice, sweet girl who practices aikido and delivers pizza as a part-time job. Johan is the title character.
  • Fuka and Fumika Narutaki of Negima! Magister Negi Magi. The former is the trouble-making class prankster while the latter's constantly worried about how the former's pranks will get them in trouble as she gets dragged along.
  • Yuma and Yuna Kashiwagi from Pani Poni Dash!. Yuma is an extreme extrovert, Yuna is an extreme introvert. Yuma goes so far as to actively sabotage Yuna's attempts at being more accepted just to keep eyes on her.
  • PandoraHearts has Alice and the Will of the Abyss. Alice is boisterous, tomboyish, ill-mannered and she likes to wear dark dresses as opposed to her sister, who is lady-like in every way and always wears light-colored dresses. Their opposite personalities, tastes, and clothing are what makes obvious which twin was in control of Alice's body in the flashbacks.
  • Persia, the Magic Fairy: Gaku is sensitive and intellectual, while Riki is stubborn and athletic. Despite the fact that Riki is like Persia personality wise, she actually realizes she likes Gaku.
  • Pokemon Omega Ruby Crimson Passion & Pokemon Alpha Sapphire Indigo Wisdom has twins Alpha and Omega. Omega is a hot-headed goofball with blue Tareme Eyes, while his younger brother Alpha is a calm boy with red Tsurime Eyes.
  • Private Actress has the twins Shuuto and Yuuto, Shiho's clients in one chapter. Older twin Shuuto is a Mr. Fanservice and a famous model, while younger twin Yuuto is just as good looking but a very awkward Shrinking Violet. Shuuto then hires Shiho to pose as Yuuto's suitor, in an attempt to help him. Massively subverted: Shiho eventually finds out that Shuuto and Yuuto are the same person and a case of Split Personality.
  • Ranma ½: Towards the end of the manga, the story introduces Pink and Link, identical twin girls from a village of herbalists that neighbours Shampoo's village of "Chinese Amazons" and who practice different branches of an unnamed "Martial Arts Herbalism", a fighting style that revolves around plants and herbalistic concoctions. Pink, who is usually smiling, is a wicked and vicious girl who loves to poison people. Link, who is usually frowning, is a kind and gentle girl who considers it her duty to heal those her sister hurts. Despite these opposite natures, they care for each other deeply and are capable of uniting with singular purpose when given motive... which means that, in their actual appearance in Nerima, they function more like Single-Minded Twins.
  • Miki and Kozue Kaoru from Revolutionary Girl Utena. Miki is polite, a model student, and a highly-skilled piano player, while Kozue is blunt, has a tendency to sleep around, and isn't very good at playing the piano.
  • Suiseiseki and Souseiseki from Rozen Maiden are very much this, both including a Tomboy and Girly Girl relationship, but with several more differing characteristic traits. Where Souseiseki is stoic, Suiseiseki is emotional. Souseiseki is serious and collected, while Suiseiseki is mischievous. Souseiseki believes it is the duty for every doll to participate in the Alice Game regardless of personal attachments, while Suiseiseki strictly opposes the Alice Game due to fear of losing her sisters.
  • Saint Seiya has the twin brothers Saga (thoughtful, wise, just until his Superpowered Evil Side kicks in) and Kanon (selfish, scheming, arrogant until he has a Heel–Face Turn.) For major irony, Saga is the Gemini Saint, with Kanon inheriting his position once Saga dies.
    • From the villainous side, the twin gods Hypnos and Thanatos. Sure, they are Creepy Twins on their own, and aside from acting similarly, they also have similar creepy eyes. However, most of the time, they are more of this trope because of their personality, as they are textbook examples of Red Oni, Blue Oni with Thanatos as the red oni and Hypnos as the blue oni. Thanatos has a much shorter fuse than Hypnos ever had, does show more emotion than Hypnos ever does, will jump into the fight if challenged, and underestimated humans in general. Hypnos, however, hates fighting and will seek ways to avoid fighting and calmly analyzes his enemies. This is even more prominent in Saint Seiya: The Lost Canvas, where they still share similar personality with their classical counterpart, where when they are defeated and sealed, Thanatos continue cursed Pope Sage pettily and furiously when Hypnos accepted his loss calmly and congratulates Hakurei instead.
  • Saint Seiya: The Lost Canvas continues this tradition with arrogant, narcissist, megalomaniac, self-centered, scheming, sleek and dainty Aspros, with the gritty and self-sacrificial Defteros. And yes, they are the Gemini Saints of their era, where Defteros had to kill Aspros because he showed his true colors, wanting to kill Defteros so that he could be the Pope. After Aspros'death, Defteros takes the Gemini Cloth with him and becomes the new Gemini Saint
  • In Saint Seiya Omega we have Gemini Paradox who has a crazy split-personality and good, loving, kind Gemini Integra. Same thing happens. Paradox is beaten by Ryuuho, and Integra picks up the Gemini Cloth afterwards during the Pallas War.
  • Samurai High School: Sakakido Kou and his sister Sakakido Tsukiko are Masculine Girl, Feminine Boy.
  • Rumiko Takahashi's one-shot The Samurai has identical twin ninja sisters, one of whom is a total nymphomaniac, while the other Does Not Like Men and Hates Being Touched. Woe be unto the person who confuses the latter for the former.
  • The Tamagotchi anime features Miraitchi and Clulutchi. Miraitchi is more outgoing, though panics easily. Clulutchi is more serious and thoughtful but isn't as sociable.
  • There, Beyond the Beyond: gives us twin boys both named Virid Visette Viridian, who are summed up quite nicely by their titles: "The Mad Prince" and "The Sane Prince".
  • To Love Ru features Momo and Nana Deviluke, which also personify this trope very well. Momo is a Yandere Chessmaster sex maniac and can talk with plants. Nana is a B-Type Tsundere Ingenue prude and can talk with animals.
  • Trigun: Vash and Knives. One is a genocidal maniac and the other is a pacifist. Vash is also all about how other people feel and love and peace, and Knives doesn't even do empathy for his twin with whom he has an effing psychic link. Vash acts like a fool whenever he can and Knives is all superiority and icy control except when his temper gets away from him. They do have a bit in common still when you get past the good and evil divide. For instance, scary if you get them mad. Stubborn as hell. Charisma. Way of speaking, when serious, is even a little similar. Pretty good, when you consider they've been separated over a hundred times as long as they were together. When they were kids in the anime, one was unusually mature and one was goofy. No prizes for which. In the manga, they were much more similar, but Vash was more outgoing and Knives was more sensitive.
  • Hamel and Sizer from Violinist of Hameln. Hamel is loud, obnoxious, and tends to spaz out, while his sister Sizer is calm, sensible, and serious. He's arrogant and loves to boast while she's a bit shy and doesn't like a lot of attention. Them being opposites is also emphasized by the fact Hamel is half-demon while Sizer is half angel, and she starts as a villain while Hamel is the hero.
  • In Wasteful Days of High School Girls, Hisui is a Shrinking Violet Nightmare Fetishist, while her twin sister Kohaku is an extroverted romantic.
  • Yu-Gi-Oh!:
    • Rua and Ruka (Leo and Luna, respectively, in the dub) from Yu-Gi-Oh! 5Ds. Rua is a rowdy, cocky duelist who overestimates himself. Ruka is quiet, introverted, and is usually the one to notify Rua when he goes too far.
    • The original series' Yugi and Yami Yugi can be counted as this if you consider them twins (of the split personality/alternate guy living in your head kind), with a dash of Sibling Yin-Yang.
    • The Tenma twins from the spin-off from the original series, Yu-Gi-Oh! R. Gekkou is mature, gentle, reasonable, and already independent from Pegasus, meanwhile Yakou is very dependent on Pegasus, that he flips his shit upon Pegasus's disappearance and takes over Kaiba Corporation to resurrect Pegasus, effectively becoming the Big Bad, spends his time on screen showing his insanity, gloating off his victory and telling the protagonists he is unstoppable or ranting about the fact that Gekkou used to be Always Someone Better to him. In their flashback too, they are this. Gekkou is cheerful and always in the spotlight, meanwhile Yakou is gloomy and forgotten or only seen as the inferior version of Gekkou. Their names reflect this too. Gekkou means "Moon Verse" when Yakou means "Night Verse". Lampshaded by Yakou himself in his match against Yugi.
  • Michele and Sara Crispino from Yuri!!! on Ice. Michele is short-tempered and uptight, while Sara is friendly and more down-to-earth.
  • In Zipman!!, Kaname is an athletic Nice Guy who gets no respect thanks to his fiendish looks. Meanwhile, his brother Koshiro is a Child Prodigy turned Teen Genius with a gentle face that everyone admires, but is incredibly rude to his twin brother in private. Even their color schemes are opposite: Kaname is known for having wild white hair that gets him labeled "White Demon", Kaname's hair is normal and black. The English blurb even refers to them as this word-for-word.

    Comic Books 
  • Jezebel and Dinah in Blacksad: Dinah is a black bear (= dark brown) with a white patch on her chest, Jezebel a polar bear (= white) with a dark patch on her chest. Otherwise, they share face structure, and body and hair type. Personality-wise, Dinah is softer and Jezebel harder, although both have it in them to do what they need if it's for righteous revenge.
  • Ember and Suntop in ElfQuest. She is a feisty, red-headed partially wolf-blooded mortal Wolfrider who is destined to become the next Chief of the Wolfriders. He is the complacent, magical, blond, immortal grandson of the mystic leader of the Sun Village and the apprentice of the equally mystic Mother of Memory. Word of God is that they actually chose these different destinies while in the womb.
  • The Flash:
    • Hyperactive, Fun Personified speedster Bart Allen (aka (Impulse) and his collected, scheming, evil clone Thaddeus Thawne (Inertia). Bart even mentions The Patty Duke Show the first time they meet, suggesting they switch places to befuddle their friends. Thad responds by punching Bart in the nose.
    • The Tornado Twins, Don and Dawn Allen, are barely shown enough to have characterization other than as Single-Minded Twins, but their individual personalities are All There in the Manual: Don is studious, levelheaded, and prefers to carefully think of a plan before taking action, while Dawn is extroverted, impulsive and will follow her gut nine times out of ten.
  • Gregory Stark and Tony Stark in Ultimate Avengers. Becomes a case of Cain and Abel.
  • In X-Men Noir, Thomas and Robert Halloway are, respectively, a know-it-all afflicted with Chronic Hero Syndrome and a crude, violent sadist. It's probably important to note that they were adopted by different families after their father died.
  • Young Avengers: Caring geek Wiccan and hyperactive bad boy Speed.
  • Swordquest: Earthworld has Gemini, who is composed of an evil white shade and a helpful black shadow.
  • In Silver Surfer: Dawn Greenwood's Establishing Character Moment is when she and her sister Eve see a shooting star (actually the Surfer), Eve wishes to go everywhere and see everything, and Dawn wishes that the star could keep going so everyone could get a wish. Eve thinks Dawn's wasted her wish since she didn't really ask for anything, and Dawn doesn't understand why anyone would want to leave their hometown. As an adult Eve's been all over the world, and Dawn still doesn't see why anyone would leave. (Too bad The Call Knows Where She Lives.)
  • The Avengers: Scarlet Witch and Quicksilver are polar opposites most of the time—Pietro is stern and stubborn and hot-tempered, his sister is gentle and patient and demure. This is especially true of their earliest appearances, where it was easier to define Wanda's personality as "Pietro's opposite". Over the years, however, Wanda has gained a stubborn temper of her own, making her more similar to her brother.
  • Kate Kane and her twin sister Beth were like this as children. Kate was aggressive, protective, and mostly stoic, while Beth was far more meek and openly emotional.
  • The Adami twins, Gabriel and Mags, in C.O.V.E.N. Gabriel has a healthy tan, blond hair, and wears bright blue and white preppy clothes. Mags has pale skin, dyed black hair, and is a black and red-wearing Goth. Gabriel acts like a smooth and friendly guy, but he's a complete Narcissist with a sadistic streak. Mags acts aloof and ruthless but has a Hidden Heart of Gold. Gabriel wants their father's approval despite the years of abuse. Mags saw getting discarded as a blessing, and only returned to protect her brother.
  • Wonder Woman (1942): Wonder Woman and her twin sister Nubia are very different in personality and appearance. Diana is a Technical Pacifist raised in an all-female paradise with a Caucasian appearance and Nubia is a Combat Pragmatist raised in a hellish male-dominated culture with a Sub-Saharan appearance.
  • Played With in PS238—Tyler's "brother," Toby, is actually a clone who's now been adopted by his parents. The most obvious difference between them is that Tyler is a Muggle Born of Mages (and secret Badass Normal) while Toby is a Reality Warper, but this trope also fits their personalities: Tyler is dry and rational, while Toby is increasingly showing himself to be extroverted and cheerful (if possibly a Stepford Smiler).
  • Bibbo Bibbowski is a tough, good-hearted bruiser in Superman comics. The Power of Shazam introduced Professor Bibbowski, equally good-hearted, but a scientist and Non-Action Guy.

    Comic Strips 
  • In the Scamp (Son of Lady and the Tramp) comics (Specifically the newspaper continuity), Scamp and his siblings are two sets of these. Twin Sisters Fluffy and Ruffy are dignified and tomboyish, respectively, while non-identical brothers Scamp and Scooter are adventurous and timid, respectively.

    Fan Works 
  • In Boy Scouts ½, the Porter twins. Kirstin is a gentle, sweet homemaker. Nicole is a greedy, ruthless money seeker.
  • In Empath: The Luckiest Smurf, Hefty and Handy are the Brains and Brawn twins but are not identical.
  • Moon Soldier features ditzy and emotional Usagi and her twin brother, the calmer and more responsible Akira.
  • In The Rabbit and the Puppy Kagami and Tsukasa from Lucky Star. Kagami is a Tsundere and Tsukasa is The Ditz.
  • Meg's Family Series has Chris and Jillian's twins, C.J. and Tilly. The former basically inheriting their parents' (lack of) brains, and the latter being a super-genius.
  • We are the Chatroom Gems has Yella and Azura Diamond, this story's versions of a human Yellow and Blue Diamond. Yella is cold, yells at a student (Lapis) for wearing a "Say hi if you're Bi" shirt, and is heavily implied to have undiagnosed IED, while Azura is emotional, has no problem with student's sexualities, and says that her depression is "apparently" obvious.
  • Used in the Brave fanvid BRAVE TWINS, which manipulates the film in order to split Merida's character into a pair of identical twins: one is a prim and proper princess while the other is a tomboyish Rebellious Princess. The plot revolves around the Tomboy Princess fighting to stop her sister from being married off because neither are ready to be married yet.
  • Downplayed in The New Adventures of Invader Zim: Steve and Viera aren't that different in personality, but their specialties are science and magic, respectively, and they initially clash about it a lot.
  • A Diplomatic Visit: Pinkie and her little twin sister Marble, as discussed in chapter 12 when she reveals she's a twin and Twilight reacts with surprise, since she's seen the Pie family portrait and Pinkie is the only pink one of her family. Pinkie replies that "Marble's a lot quieter and shyer and grayer than I am. Kind of like a mix of Mac and Fluttershy and a rock."
  • Leave for Mendeleiev features Chloe and Amber Bourgeois. While Chloe prefers using her father's connections and funds to coast along taking everything she feels obligated to have, Amber has been raised by their mother to work hard and earn things rather than relying upon the station she was born into. Naturally, Chloe believes that Amber has screwed her out of her mother's love by her very existence, rather than considering that it might have anything to do with how she's always cutting corners and trying to trick and bully her way into getting everything she wants handed to her.
  • A common Fanon in the Persona 3 fandom is that the male and female protagonist options are twins and that the chosen protagonist is the Sole Survivor of the car crash that killed their parents. In stories where the two are portrayed as twins who both survived, it's common for the male protagonist to be portrayed as an example of the Sugar-and-Ice Personality type, who doesn't open up to the rest of SEES for quite some time, whereas the female protagonist is portrayed as a Stepford Smiler, who doesn't show off her own inner despair no matter how she feels except in the Darkest Hour.
  • A Pairing Of Souls features Shen Jiu adopting Shen Yuan as his younger twin brother. If Shen Jiu is an unrepentant asshole who constantly plans for the worst and tries to hide his vulnerable soft center, Shen Yuan is openly nice and personable to the point of naivety but will display a steely spine when his buttons are pushed.
  • Pokémon Reset Bloodlines has two pairs of Half-Identical Twins with this dynamic, overlapping with Foolish Sibling, Responsible Sibling:
    • Elwood and Aideen, the twins who got the remaining starter Pokémon from Professor Oak on the same day as Ash and Gary. Elwood is the calmer, more mature and levelheaded of the two, while Aideen has a fiery and more outspoken personality that her older brother often needs to rein in.
    • The Lono twins in Alola, Frax and Velvet, invert it: Frax, the male twin, is a Keet who is a bit Book Dumb and tends to jump headfirst into situations, while his sister Velvet is more responsible, does better at school and tends to think things through. This even extends to their Pokémon, as Frax is noted to have befriended most of his mons with ease and they just followed him, while Velvet had to catch hers through battles the traditional way.
  • All Mixed Up! has Carlos and Carol, who are very different from each other in personality, purpose and appearance despite being siblings. Carlos is the sentient paper fortune teller that runs the Language Room, who is depressed, forgotten, and has his colors inverted to highlight how tainted he is. Carol, the sentient paper fortune teller that runs the Math Room, is much more eccentric, bright, cheery and helpful, with her displaying a wide array of colors and agents coming to her often whenever they get stumped on math problems.
  • The Hero Strikes Back has Luke and Leia as mentioned below in Live Action, and a particular quirk in their training, where Luke has a preference for meditation and communing with the Force, while Leia is more active and prefers practicing Lightsaber forms. When being punished for teasing each other, Anakin has Luke work on his forms while Leia is forced to practice her meditation.
  • Harry and Danny in Life's Curse, Death's Gift share a temper and a Chronic Hero Syndrome, but being Separated at Birth ensured they grew quite distinct from each other. Harry lashes out in anger against injustice and was eager to drop his abusive relatives to join the Wizarding World, Danny is more cautious about antagonizing other people even when they clearly are in the wrong and is upset to leave his loving parents and sister in order to study magic at Hogwarts. It causes a lot of friction between them.
  • In Kaiju Revolution, one Mothra larvae is brown with blue eyes and is friendly and curious, while the other is black with red eyes with a more solitary personality and more violent when provoked.

    Films — Animation 
  • Quest for Camelot has the serious and snooty Devon and the boisterous, fun-loving Cornwall. Unfortunately, they're not only Polar-Opposite Twins, but they're also Conjoined Twins, and they're unable to use their shared body to fly or breathe fire until they learn to get along near the end of the movie.
  • Spirited Away: The identical twin sisters Yubaba and Zeniba. Yubaba is selfish and materialistic; Zeniba, while vengeful at first, turns out to be kindly and humble. It's worth noting, though, that the sisters aren't really polar opposites — Yubaba has an introspective side (musing about the nature of humanity and its greed), treats her workers relatively well, so long as they work hard, and genuinely loves her son Boh to the point of spoiling him. Similarly, Zeniba can be just as nasty as her sister — one of her first lines to Chihiro is a threat to rip the girl's tongue out if she says anything about her presence. Miyazaki seems to be deconstructing this trope with the characters, pointing out that unlike fairy tale villains, most adults are both bad and good, with positive and negative qualities alike (a fact suggested by Chihiro eventually calling both sisters "Granny").

    Films — Live-Action 
  • Constantine (2005) has the aggressive cop Angela, and her twin sister Isabelle who is The Ophelia.
  • Dead Ringers has identical twin gynecologists Elliot and Beverly Mantle. Elliot is smooth and a ladykiller while Beverly is a Shrinking Violet. They are so identical that the only way Beverly can have sex is for Elliot to seduce the woman then substitute Bev for himself at the very last moment in the bedroom.
  • Double Trouble: David is an honest cop with slobby habits, and his brother Peter is an unrepentant cat burglar with a taste for culture.
  • EuroTrip: The twins Jenny and Jamie are pretty different. Throughout the movie, the phrase "worst twins ever" is repeated.
  • Glass Onion: One twin is a high-flying big-city Disruptor, the other is a humble small-town teacher. The latter Brand twin, Helen, has to impersonate her glamorous executive sister Andi, and is very uncomfortable throughout.
  • A Haunting at Silver Falls: The Dahl twins. When Jordan goes to meet with their father, Holly is described as having been a good Christian girl, while Heather acted out because she was afraid of being forgotten.
  • Hellboy II: The Golden Army has Prince Nuada and Princess Nuala; while they are Half-Identical Twins in appearance, Word of God says they were intended to be as different as night and day in all other respects.
  • In License to Drive, protagonist Les (Corey Haim) is laid back, fun-loving, and just a typical teenage boy in most respects. His twin sister Natalie is bookish, serious, and depicted as being rather a stick in the mud. The trope is lampshaded when Natalie aces her written driving exam and Les fails his. He manages to knock out the computer system before his grade can be recorded; this leads the woman running the exam to just allow him to go ahead with the practical exam. Her logic?
    "How different can you really be from your twin sister?"
  • New York Minute: Rock star wannabe Roxy and Young Republican Jane.
  • The Parent Trap (1961): Susan and Sharon. Sharon is the Proper Lady of the two, while Susan is a hip tomboy. In both the original and the remake, this is at least partly the result of their parents' Solomon Divorce, with the ladylike Sharon/Annie raised by their mother while the scrappier Susan/Hallie was raised by their father.
  • The Parent Trap (1998): The twin girls, Annie James and Hallie Parker, are completely different. Annie is calm, reserved, and rather uptight while Hallie is relaxed, loud, outgoing, and a Big Eater.
  • The Pretty One: Laurel and Audrey are identical twins. Audrey is self-assured, confident, and popular. Laurel is reclusive and is convinced that she is the inferior of the two.
  • She's the Man: Sebastian and Viola. He's artsy and musical, and she's athletic and loves soccer. It's even lampshaded near the end of the movie.
  • Shock Treatment: The Reveal is the fact that this is the case of Brad Majors and Farley Flavors, who were separated as infants when their parents died.
  • Star Wars:
    • Luke Skywalker is a blonde, blue-eyed, calm, mild-mannered, naïve, mystical, religious Jedi Knight. Leia Organa (née Skywalker) is a brunette, brown-eyed, quick-tempered, sharp-tongued, world-wise, shrewd, practical politician. Interestingly, they parallel their parents' personalities from the Prequel Trilogy, with Luke taking after their mother Padmé while Leia takes after their father Anakin.
    • The Star Wars Legends show Luke having trouble teaching Leia to be a Jedi. What worked for him (he has a lot of Jedi qualities) won't work for her. Hot temper is not something a Jedi knight ought to have. It also doesn't help that she's pregnant while trying to learn, and is constantly busy with politics. In the reboot, Luke ended up training Leia to become a Jedi without much trouble as seen in The Rise of Skywalker.
    • Also in Legends, Han and Leia's first children are the twins Jaina and Jacen, who like Luke and Leia also partially take after their opposite-gender parent. Jaina, like Han, is blunt, mechanically-inclined, and an excellent pilot, while Jacen, like Leia, is more thoughtful and focused on responsibility. It becomes much more tragic when Jacen turns to the dark side, emulating their grandfather Darth Vader, while Jaina grows more patient, and in the end, Jaina has to kill Jacen.
  • Through Black Spruce: Despite them being identical, Annie and Suzanne are opposites in many ways. Before going to search for her sister, Annie seemed happy living in their rural home town, which Suzanne hated, leaving it for Toronto. Annie wears more masculine clothes mostly, while Suzanne became a model. Suzanne had gotten in trouble with drugs and an abusive boyfriend; conversely Annie is a sober, very responsible person overall.
  • Twins (1988): The premise of the movie is that Arnold Schwarzenegger and Danny DeVito play genetically-engineered twins, with Julius Benedict (Schwarzenegger) being the product of attempting to make the perfect man and Vincent Benedict (DeVito) being all the "genetic garbage" they were weeding out of Julius.
  • Underworld (2003): In Evolution, Markus and William Corvinus are twin brothers who respectively became the first vampire and first werewolf. One is scheming and pompous, the other is near-mindless and savage. Unusual in that we never learn if they'd been genetically identical or not, as William is already locked permanently in his wolf-man form at the time the film is set, but they probably were.

    Literature 
  • Artemis Fowl: Beckett is cheerful, ditzy, and much prefers physical activity, while Myles is basically a toddler version of Artemis.
  • Baka and Test: Summon the Beasts: the Nice Guy Hideyoshi and the Jerkass Yuuko. Further differences include that Hideyoshi is not smart enough to qualify past Class F (the bottom of the barrel), while Yuuko is in Class A (the best), and Hideyoshi is a beautiful singer but Yuuko is completely tone-deaf.
  • Polgara and her twin Beldaran in The Belgariad series. The former is dark-haired, has a grim disposition, and grew up to be an immortal sorceress. The latter was blonde, had a sunny disposition, and married young to become the queen of an island kingdom and bear the royal line which would deliver the child of prophecy, before dying young of pneumonia. As Polgara's biography explains, the two were originally much more similar, but their mother tampered with them in the womb to prepare them for their destinies.
  • Brimstone Angels: Main character Farideh is broody, angsty, and painfully self-aware that her heritage as a Tiefling (human-devil hybrid) marks her as an outcast. Her twin sister Havilar is cheerful, friendly, and if she has any awareness of how people view her and her sister, she never shows it (much to Farideh's chagrin). On top of that, Havilar is a traditional Action Girl, who took to their adoptive father's martial training much better than Farideh, whose decision to become a warlock sets the plot in motion. Part of Farideh's angst is that she's convinced people want her to be more like Havilar.
  • In Castle Hangnail, Molly is a Wicked Witch who likes bats and earwigs and animal skeletons and dresses in black as much as her parents will let her; Sarah loves pink and glitter and ponies, goes around making insipid comments to "Mr Bluebird" and "Miss Rabbit", and regularly visits the residents of the local retirement home.
  • Occurs in Gordon R. Dickson's Childe Cycle series of novels and short stories, in the characters of Ian and Kensie Graeme. Both are Dorsai (Proud Warrior Race Guys). Ian is the epitome of the Warrior - seemingly aloof and intimidating by his sheer presence. Kensie is his polar opposite - warm, caring, social - everything that Ian is not. In the stories, it's implied that together the two make up one individual. Ironically, it's Ian who's irresistible to women - without realizing it.
  • Double Trouble: Randi likes red, soccer, and is outgoing; Sandi likes pink and purple, schoolwork, and is rather quiet. They do agree that their two-year-old brother is a pill.
  • The Brothers Majere; easy going, heroic Boisterous Bruiser Caramon and intense, morally ambiguous Squishy Wizard Raistlin in Dragonlance.
  • Deliberately invoked in Raise the Titanic!. Pitt has been hunting for Silver and Gold, a pair of Soviet spies on the NUMA Titanic project. He's figured out Gold is Drummer and while much of his background was hidden, the FBI were able to discover he was one of a pair of non-identical fraternal twins. Sandecker says it must have been easy for one twin to lead to the other but Pitt corrects him that the two were very smart and played as total opposites in personality while rarely hanging out so no one would make any connection. Pitt himself was stumped until Drummer was going crazy about a submarine in trouble and Pitt realized his twin had to be one of the three men on board. Since one was eight years older than Drummer and the other Mexican, that left Merker.
  • Lampshaded in The Legendsong Saga. Glynn and Ember are twins who are both physical and personality opposites. Their only physical similarity is the same coloured eyes (yellow); personality contrasts include Ember�s musical talents and Glynn being tone-deaf.
  • In Dragon Bones, Erdrick is the shy, sensible twin, Beckram is a ladies' man and troublemaker. They look so alike that Beckram often persuades Erdrick to take his place at boring social occasions.
  • Shaun and Shem from Finnegans Wake probably count, but it's hard to figure out which is which, due to the book changing their names constantly. All you need to know is one is boring and dull, and the other is really creative and artistic.
    • Specifically, Shaun is dull and conformist but successful in society, while Shem is a slovenly outcast but also an artistic genius.
  • Durarara!!: Mairu and Kururi Orihara actually invoke and subvert this. They actually flipped a coin to decide which girl got which trait. Strangely enough, their roles (Mairu as the Genki Girl and Kururi as The Quiet One) switch when they're on the internet. Despite how differently they act, the subversion lies in the fact that they don't actually consider themselves as separate people.
  • Owen and Porter from First Person Plural by Andrew Beierle. Owen is a book-reading drama nerd. Porter is a cheerleader-dating football player. Owen is gay, and Porter is straight. They are also conjoined twins sharing one body with two heads. This is incredibly awkward considering that Porter married a homophobic woman.
  • Nicky and Alex Katsopolis, in the Full House spinoff book series, grow up to be different; one is tough, one shy. Interestingly, in one episode of the show, their father was concerned that their personalities would turn out the other way around.
  • The Eiran twins Eamonn and Grainne from Kushiel's Dart by Jacqueline Carey.
  • The Marlow twins Nicola and Lawrie from the Marlow series by Antonia Forest. They are so identical in looks that they are easily mistaken one for the other by people who don't know either of them well, but so different in mannerisms, behaviour, and speech, that anyone who does know them well can tell them apart immediately. Lawrie, a skilled actor, can more easily pass for Nicola when she wants to than Nicola can for Lawrie - a plot point in one of the books.
  • In the original Story Lottie and Lisa by Erich Kästner (aka "Das doppelte Lottchen"), the titular twins had extremely different personalities, which made their place change all the more hilarious, and later difficult. Lisa was a cheerful, bratty tomboy while Lottie was a sensible, serious, prim, and proper girl who behaved almost like a grown-up. Most film versions omit this aspect.
  • Eve and Alexandra in Master of the Game are a Cain and Abel crossover version — Alexandra is sweet, honest, and innocent, with no ambitions towards running Kruger-Brent, Ltd. Eve is consumed by ambition and the desire to be the center of everyone's attention, and starting from the age of five she's determined to kill and/or ruin Alexandra, who's in the way. (Incidentally, author Sidney Sheldon co-created the much gentler Trope Namer years before.)
  • Abby and her twin sister Anna of The Baby-Sitters Club. Anna is musical, bookish, and introspective; Abby is athletic, noisy, and enjoys babysitting. About the only things they have in common are that they both have scoliosis and poor eyesight.
  • Dawn and Eve of Piers Anthony's Xanth are literally as different as day and night, from the fact that Dawn is red-haired and green-eyed and is like the morning, while Eve is black-haired and eyed and is like the night.
  • Belle and Samuel from Rolitania are very different, indeed. Belle is popular, sociable, and has thousands of Instagram followers. Samuel, on the other hand, is the Butt-Monkey of the class, a former NEET, and can hardly speak for himself when confronted.
  • Both the books and TV version of Sweet Valley High, with the Wakefield twins Jessica and Elizabeth.
  • The Tortall Universe gives us:
  • In Pierce's Circleverse, Nia the shy, retiring one (with woodworking magic) and Jory the hyperactive, overeager one (with cooking magic).
  • Tatterhood and her unnamed twin sister from the Norwegian fairytale are a perfect example of this: Tatterhood is an ugly but spirited princess who rides on a goat and singlehandedly beats up hordes of trolls and witches, while her sister is a beautiful but helpless Damsel in Distress. Despite this, the twins are inseparable and get along famously.
  • Amoret and Belphoebe in The Faerie Queene: The former was adopted by Venus, the Goddess of Love and Beauty, and became a Proper Lady. The latter was adopted by Diana, the Goddess of Maidenhood and the Hunt, and became an Action Girl.
  • Robert L. Stevenson's The Strange Case of Dr. Jekyll and Mr. Hyde: Dr. Jekyll and Mr. Hyde may be considered a variation of this trope.
  • Emmeline and Adeline March from The Thirteenth Tale, who are totally passive and excessively violent respectively.
  • The twins in Flowers in the Attic: Cory is quiet, polite and gentle. Carrie is loud, adamant, and forceful. Her personality shifts dramatically after Cory dies, however.
  • Web Serial Novel Correspondence From the Goddess has Lydia Devin, the main character, a snarky aimless twentysomething who slowly gains divinity-level powers over the course of the story, and Elana Devin, her twin sister, who is perfect as perfect can be but doesn't seem to have any powers at all.
  • Jacqueline Wilson's Double Act has Tomboy Ruby and Girly Girl Garnet. This becomes more apparent when Ruby cuts off her braids to distinguish herself further from her sister.
  • Squirrelflight and Leafpool from Warrior Cats, especially when they're apprentices. Squirrelflight is sharp-tongued and energetic, and becomes a warrior, while Leafpool is calm and more reasonable, and becomes a medicine cat. They're even compared directly to fire and water once.
  • The Millenium Quadrology has Lisbeth and Camilla Salander. Lisbeth is a brilliant hacker with No Social Skills who stands up for abused women and children and has a strong moral code. Camilla is highly manipulative, does not care about those she considers "weak", and has very little in terms of morals being the leader of a Russian crime syndicate.
  • Cilla and Tina from I Miss You, I Miss You. Cilla is down to earth and is interested in directing theater while Tina is mainly interested in boys and can be quite petulant.
  • The Jackie Collins novel Chances has Lucky and her brother Dario. Lucky has dark hair and a vigorous personality, and she's extremely ambitious. Dario is blonde and a slacker, more interested in his Porsche than the family business.
  • Tobi and Toklo from Seeker Bears. Tobi is timid, quiet, and delicate, while Toklo is adventurous and more than a little grumpy.
  • Star Wars: Scoundrels: Bink and Tavia Kitik are amazingly identical physically, but couldn't be farther apart in personality. Bink is bold, outgoing, and a risk-taker, a Classy Cat-Burglar who's very good at infiltration and the physical aspects of the robbery. Tavia is quieter and less confident, but very skilled with electronics and computers, acting as an able Mission Control and support for her sister. Tavia makes herself sick with worry every time Bink goes on a job, is even more nervous when she has to go into the field herself and would like for them to go legit, but every time she tries Bink pulls them back into the life. Bink, for her part, considers Tavia naïve; her "legitimate" jobs have all involved someone taking advantage of her to accomplish something even shadier than thievery with her skills.
  • I've Been Killing Slimes for 300 Years and Maxed Out My Level has the slime spirit twins, Shalsha and Falfa. The former is cold, bookish, and reserved, and the latter is warm, sporty, and outgoing.
  • Fairy Oak: Vanilla and Lavender are this as well as being a Night and Day Duo. Lavender was born at the stroke of midnight while Vanilla was born exactly twelve hours later. Vanilla is outgoing, friendly, and friendlier than her sister, who in turn is impatient, a bit aggressive, and Not a Morning Person. The fact that they both have Personality Powers only drives the point home since Vanilla's magic is light-based and Lavender's is drawn from darkness.
  • Harry Potter has Parvati and Padma Patil. Parvati is the more outgoing twin, while Padma is the more studious twin. It's noted that the two are sorted into different houses, Parvati in Gryffindor and Padma in Ravenclaw, when family members are usually sorted into the same house.
  • Anansi Boys: Fat Charlie and Spider start out as this. Fat Charlie is the mundane, responsible, and anxious twin with the dull accounting job and absolutely no knowledge of his family's supernatural background — while Spider is an impossibly cool, impossibly confident party animal who goes through life without a care for anything or anyone and is seemingly the only one of the two to inherit their father's Trickster God powers. It's eventually even revealed that they are not technically twin brothers, but rather Spider is Charlie's Literal Split Personality, his magical half that was cast out of him by an old lady that he angered as a child. Although Charlie concludes that they have both grown into their own whole persons from it and the two still regard each other as brothers. As the story goes, Spider becomes more humble and responsible due to experiencing consequences for his actions for the first time in his life and learns how to care about other people, while Charlie learns how to be more confident and carefree and how to harness his own Trickster God powers. So by the end of the story, the twins are basically on equal footing. With Charlie possibly being the more confident and magical out of the two now, but still a far cry from the total opposites they were at the start.
  • In the Selerbergiada short stories by Ewa Białołęcka, Rita and Ricky von Selerberg are fraternal twins, and they can be mistaken for each other when seen from the behind. But while Rita trains dwarven box and hides her fashion-related interests (because she considers herself too unattractive to look anything but ridiculous in cute clothes), Ricky is definitely a lover, not a fighter - his main interests remain dating and music (dwarven metal, admittedly). Most of the time she's the mastermind behind whatever they do, while he just goes along and snarks.
  • Xanadu (Storyverse): One short story describes a pair of twins, one a picture of politeness and courtesy around adults but wicked, false and demanding around other children while the other is sullen, quiet and stubborn but trustworthy, sincere and devoted to helping others. At the convention, the wicked twin had gone as an angel and the good one as a devil, but after the Change they only remain in these forms for a moment before they feel a pair of immense hands pick them up and set them down in each other's bodies.
  • A Song of Ice and Fire:
    • Aerea and Rhaella Targaryen, the daughters of Aegon the Uncrowned. As children, Aerea was shy and timid, while Rhaella was bold and willful. Growing up, however, their personalities dramatically switched, as Aerea became a wild and outspoken girl, while Rhaella turned into a studious girl who devoted herself to religion by becoming a septa. Because of this discrepancy, there are rumors that the girls switched places when they attended their uncle Jaehaerys' coronation at Oldtown.
    • Baela and Rhaena Targaryen, Daemon's daughters with Laena Velaryon, were identical when they were young, but by the time they turned sixteen they no longer looked alike. Short-haired Baela was an energetic tomboy who loved to ride and fly, while long-haired Rhaena was a Proper Lady who loved dancing. Baela was a warrior during the Dance of the Dragons and personally dueled Aegon II using her dragon, while Rhaena, who didn't get a dragon until she's come of age, mostly stayed out of the conflict.
  • In The Ickabog, the two baby Ickabogs at the end as they respond to how they're treated as they're born. The first hates humans as they were trying to kill it and its Icker. The second is friendly as things had calmed down when it was born a few minutes later.
  • Sweet & Bitter Magic: Although identical, Tamsin and Marlena were very different otherwise. Tamsin is the most powerful witch in her generation, able to do spells with ease which exhaust others. Marlena though was very weak, and doing even simple spells put her in the infirmary, even into a coma. She grew to hate Tamsin for accidentally killing a friend of hers. Tamsin though had caused this as a result of a spell to save Marlena because of how much she loved her, which caused her to risk everything.

    Live-Action TV 
  • Power Rangers:
    • Fraternal twins Madison and Vida Rocca, the Blue and Pink Rangers, from Power Rangers Mystic Force. Madison is calm and thoughtful, V is on a permanent sugar high and goes rushing into things with a mad grin.
    • The Blue Ranger Theo Martin and his civilian identical twin brother Luen from Power Rangers Jungle Fury. Theo is calm, focused, and studious, while Luen is adventurous and outgoing.
  • Sister, Sister, with Tia and Tamera Mowry. Separated at Birth, Tia is the mature, smart, and responsible one, while Tamera is irresponsible and Book Dumb. Their characters in the Disney film version of T*Witches are basically the same.
  • Two of a Kind and So Little Time, both with Mary-Kate and Ashley Olsen — the twins also played this up in most of their DTV movies as well. Mary-Kate typically played the wild and tomboyish twin while Ashley usually played the more responsible and more feminine sister.
    • One of the only times that this was ever reversed was in Winning London (the movie where they went to England), where Mary-Kate was Chloe Lawrence, a serious and competitive overachiever, while Ashley was Chloe's laid-back/fun-loving twin sister, Riley.
  • A few Disney Channel shows:
  • Friends gives us identical twin sisters Phoebe and Ursula. Phoebe is a (mostly) light-hearted and cheery Cloudcuckoolander, while Ursula is mean-spirited and thoughtless, and, in Phoebe's words, a "real high-powered, driven career-type" (she's a waitress).
  • The '80s sitcom Double Trouble: Identical twins Jean and Liz Sagal (younger sisters of Katey Sagal of Married... with Children) play identical twins Kate and Allison. Allison is serious and bookish; Kate is happy-go-lucky.
  • Big Good Jacob and Big Bad The Man in Black, Jacob's fraternal twin from Lost.
  • Jerome Valeska and his identical twin Jeremiah are this in Gotham. Jerome is a Large Ham psycho, Jeremiah is cold and meticulous. Their actor even compared them to a chainsaw and a scalpel.
  • Scoundrels, Cal and Logan West, an incompetent burglar/criminal, and an intelligent defense lawyer, respectively. The characters are so different, you can barely tell they're played by the same actor.
    • This is a remake of a New Zealand show, Outrageous Fortune, with Van and Jethro West in the same roles (also played by the same actor).
  • The good and evil brothers in The Wanderer. Despite both being played by Bryan Brown it isn't at 'all' difficult to tell Adam from Zachary, the evil cackle gives it away every time.
  • iCarly: Sam and Melanie Puckett. The former is almost permanently grouchy, borderline sociopathic, and sarcastic, while the latter is upbeat, outgoing, and perky. Notably, Carly says she can't believe that they're sisters. They agree.
  • Lee and Leah, the Separated at Birth twins from the British teen show The Gemini Factor, are a rebellious boy and a teacher's pet girl, with a sprinkling of Twin Telepathy. They also tended to wear black and white, respectively.
  • Katie and Emily Fitch from Skins. In the beginning, Katie is portrayed as the shallow, popular, boy-obsessed Alpha Bitch, and Emily as the sweet, socially-awkward Shrinking Violet (and closet lesbian) who does everything her bossy sister says until she comes out of the closet and begins dating her girlfriend Naomi. But when both girls suffer a series of severe personal setbacks they handle it very differently, with Katie gaining a lot more depth and maturity, having to hold the family together virtually on her own, and Emily becoming more and more angry and destructive.
  • Cathy and Patty Lane from The Patty Duke Show. "But they're cousins, Identical cousins all the way. One pair of matching bookends, Different as night and day."
    • SCTV parodied the show, running promos for "Identical Bellhops", "Identical Cheese Hostesses" and "Identical OPEC Oil Ministers", all shoehorning their titles into the familiar theme song.
  • Sutton and Emma on The Lying Game. Sutton is a Book Dumb Rich Bitch Ice Queen Alpha Bitch while Emma is sweet, caring and intelligent. The only things they have in common are a love for tennis and for Ethan.
  • The Big Bang Theory has Sheldon and Missy Cooper. Missy is an athletically built, charismatic, Southern Belle whom their mother once described as being "dumb as soup." Sheldon is the page image for Insufferable Genius, a nerd, with No Social Skills to the point he does not understand typical human interaction.
  • Patricia and Piper Williamson from House of Anubis. Patricia is the bold, Tomboyish, defiant and rude one; Piper is highly talented in piano, very sweet and polite, and much less inclined to make big moves out of fear of failure than her Determinator sister is. It's pretty obvious when you compare their crushes - Patricia likes Jerk with a Heart of Gold Eddie, while Piper likes the Nice Guy Class Clown Alfie.
  • Max and Phoebe of The Thundermans share only one trait: their superpowers.
  • Bewitched had identical cousins Samantha and Serena. Sam was (mostly) happy maintaining the Masquerade as a suburban housewife, while Serena was a wild party-girl who regularly abused her magic.
  • Jeannie and her sister on I Dream of Jeannie. Jeannie is a blonde who wears pink, and while she can be selfish and impulsive, is generally sweet and wants the best for Major Nelson. Her sister is a brunette who wears green and is a lot more malicious, self-centered, and sexually aggressive (constantly going after Tony, who's not interested), and causes a lot of problems whenever she shows up.
  • Turns out that every Spellman family member is born with a Polar Opposite Twin in Sabrina the Teenage Witch. The most glaring example is Zelda and Jezebelda. Zelda is an intellectual who values knowledge and culture. Her twin, on the other hand, speaks like a Southern Belle and describes herself as a "bad bad girl".
  • Protagonist Chip of Baskets is creative, sensitive, and withdrawn but also bitter and pretentious, while his brother Dale is bombastic, charismatic, and optimistic but also cruel and competitive.
  • Saba's twins in Savage Kingdom are a curious, feisty female and a shy, laidback male. The latter's shyness works to his advantage when the twins come across a lioness. The more adventurous female is killed because she didn't hide as well.
  • Control Z: The Alexander twin sisters are very different, as Sofia lampshaded in the first episode. Maria is nice, selfless, and more supportive, while Natalia is derisive, self-centered, and more independent.
  • The Wilds: Rachel and Nora not only look quite distinct, but they have very different personalities. In Rachel's case, she's a more outgoing athlete who's extremely focused on that and seems neurotypical. Nora has social anxiety from implied autism and is a shy, quiet, bookish girl overall.
  • Himmelsdalen: Helena is staid and more conservatively dressed. Siri is wilder, adventurous, with a more relaxed style. It turns out Helena is also neurotypical, while Siri's a sociopath.
  • Scoundrels (2010): Cal and Logan are identical twins with contrasting personalities: Cal is a thuggish deadbeat who wants to become a career thief and conman like his father, while Logal is a professional and driven lawyer who doesn't want to get involved in crime.
  • In one episode of Modern Family, Luke and Manny date a pair of identical twin sisters who basically had the same personalities as them, except the dumb one liked Manny and the smart one liked Luke. The boys wanted to switch to be with the more compatible sister but the girls refused, although they did offer to make out with them.
  • The main characters of Cómplices al rescate are the Separated at Birth twins Silvana and Mariana. Silvana is the vain, snobby and bratty twin who was raised by her rich foster parents while Mariana is the humble, sweet and cheerful twin who was raised by her biological family in a very small town and is much poorer.
  • Elementary: The Ellison twins from "While You Were Sleeping". Rebecca Ellison is a kind-hearted woman that wanted to welcome her late father's illegitimate children into the family. Her twin sister Yvette is a selfish, Manipulative Bitch who was so determined to inherit everything that she killed the half-siblings and was plotting to kill Rebecca until Sherlock Holmes intervened.

    Myths & Religion 
  • Artemis/Diana and Apollo from Classical Mythology. She's associated with the wilderness and hunting, he's the god of arts and civilization. She protects girls; he protects boys. She eventually became associated with the moon and night while he got the sun and day. She made a vow of eternal chastity, and he...did not. They both have a connection to archery, however, and they both love their mother Leto. As Niobe unfortunately found out, they love her so much they will kill people on her behalf.
  • Hebrew Mythology has Shachar and Shalim, the twin gods of dawn and dusk.
  • In The Bible, we have Jacob and Esau. Esau is the older one, and he displays a Carpet of Virility and is a skilled hunter. Jacob is younger, isn't so hairy, and prefers to use his brains over brawn. This eventually enables him to receive Esau's birthright. They were at odds with one another before they were even born—God explains to their mother that her difficult pregnancy came from them fighting in the womb. They do manage to make peace... several years later.
    • According to Jewish tradition, Jacob's cousins/wives, Rachel and Leah, were also twins and had similarly disparate personalities, the former being a quiet introvert, the latter more gregarious and emotional.
  • Nissien and Efnissien from the Mabinogion. Their names literally mean "peace" and "not-peace" which, funnily enough, highlights the main theme of the story. Not much is said of Nissien. His sociopath brother, on the other hand...

    Theatre 
  • In The Venetian Twins by Carlo Goldoni, this trope is combined with theme of the accidental twin switch. The twins are completely different in their way of acting and talking; one is considered well-mannered and intelligent, while the other is seen as tactless and stupid.

    Tabletop Games 
  • Dungeons & Dragons:
    • Forgotten Realms: A number of the gods in the pre-Fourth Edition setting are opposed siblings, although they usually look noticeably different — Shar and Selûne (the moon and the dark), Tymora and Beshaba (good and bad luck), Vhaeraun and Eilistraee (drow thieves, often male, and redeemed drow, often female).
    • Ravenloft: Laurie and Gennifer Weathermay-Foxgrove are identical twin monster-hunters and proteges of Rudolph van Richten. Laurie is an exuberant tomboy, fencer, and pistoleer who dresses like a man, while Gennifer is a demure, sober-minded wizard with closets full of conservative dresses. Also, Gennifer may be an unwitting lycanthrope.
  • Warhammer Fantasy: The elves really seem to be prone to this trope.
    • The High Elf heroes Tyrion and Teclis are identical twins with radically opposing personalities and skills. Tyrion is proud, fair, noble, athletic, and well-spoken, while Teclis is dark, feeble, scholarly, caustic-tongued, and bitter. The one is the greatest warrior and commander of his race but has no talent for sorcery, the other is a poor fighter but the most powerful living wizard in this age of the world.
    • Another example can be found in the 5th edition Tears of Isha campaign pack, which focuses on the war between Calaidan and Kaldor, twin scions of the High Elf house Coraith, one of whom defects to Naggaroth and becomes an evil Dark Elf while the other inherits the family estates and continues their proud High Elven traditions.
    • The Wood Elves have Naestra and Arahan, polar opposite personalities in identical bodies produced when Naestrahan was split into two women due to an unknown event deep in the woods of Athel Loren. Naestra is peaceful, kind and compassioante, while Arahan is wild-tempered and aggressive, and revels in death and battle.

    Toys 
  • Not technically twins, but Monster High has Jackson Jekyll and Holt Hyde, complete opposites who share a body. While Jackson is quiet and nerdy, Holt is outgoing and upbeat. They even used to have a "night and day" system of who was the one in control, although that has since changed.
  • The Bratz Twins characters Phoebe and her identical twin Roxxi are this way. Phoebe likes soft colours and dresses poppy while her sister Roxxi likes dark colours and dresses punkish. Their jingle even calls them "Sweet and spicy".
  • Skyburst and Stormclash, twin sisters from Transformers. Skyburst is a pacifist who only engages in combat when absolutely necessary after all other solutions have failed, Stormclash is a ruthless warrior whose only response is to fight and show no mercy.

    Video Games 
  • Django and Sabata from Boktai fit this trope perfectly: not only do they have opposite demeanors, but their powers are the exact opposite of one another.
  • The titular Cuphead is known for being the hot-blooded, courageous, and sometimes devious older twin brother. "A shoot-first-and-ask-questions-later type of guy." Mugman is known for being the good-humored, industrious, and rascally younger twin brother. "Calculated in battle and a tricky adversary." While both of them have a rebellious streak, Mugman does try to stop Cuphead when the latter gets too overconfident when betting against the Devil.
  • The fourth installment of Detectives United introduces Agent Dorian Brown's Evil Twin, Mortimer. Mortimer is calculating, self-serving, and perfectly willing to use or hurt other people to get what he wants. Dorian is honest, loyal, strives to do the right thing, and will defend those closest to him to the death if need be.
  • The Devil May Cry series has hothead Dante and calculating Vergil.
  • Dragon Age II has Hawke's younger brother and sister, fraternal twins Bethany and Carver. Carver is a tall, burly swordsman with an abrasive and somewhat irritable personality. Bethany is a petite mage and is gentle, sweet-natured, and friendly to everyone. They even have opposite feelings toward their elder sibling; each companion in the game has a relationship meter, and Bethany's starts with a heavy tilt toward the friendship end, while Carver's starts with a heavy tilt toward the rivalry end. The game itself also draws attention to their Red Oni, Blue Oni personalities, as the background of Carver's icon is a reddish-brown while Bethany's is a vivid blue.
  • Elden Ring has several sets.
    • Malenia and Miquella. Malenia is a redheaded Statuesque Stunner of an Action Girl who's best known for her unparalleled swordplay, while Miquella was blonde and a Non-Action Guy as he was cursed to eternal childhood, but was The Archmage and an excellent inventor. The two work together to support Miquella's plan to become a full god and nurture the Haligtree as a home for those outcast from the Golden Order.
    • There's also Mohg and Morgott, the Omen twins. You wouldn't know the two were related just from seeing them together, as Mohg barely looks humanoid while Morgott looks like a tall human with horns sprouting around his body. Morgott is also a staunch supporter of the Golden Order despite its hatred for Omens and currently being in shambles, while Mohg has embraced the defilement he was born into and worships an Outer God called the Formless Mother, and with her aid created Blood Magic.
  • The Elder Scrolls V: Skyrim has fraternal twin brothers Vilkas and Farkas in the Companions guild, who are a combination of this trope and Red Oni, Blue Oni. Vilkas is very intelligent but abrasive, hot-blooded, and not especially friendly to new arrivals. Farkas is generally regarded as being dumb muscle, but much more laid back, difficult to anger, and sweet-natured. They are both good people, however, and they love each other and their guildmates.
  • Final Fantasy series:
  • Fire Emblem:
    • Edain and Brigid from Fire Emblem: Genealogy of the Holy War. Edain is a sweet White Magician Girl who didn't train as an Action Girl because it simply didn't fit her kind nature, while Brigid is a stubborn Lad-ette that was first kidnapped and then raised among pirates.
    • Fire Emblem: The Binding Blade introduces another pair in Lugh and Raigh. Lugh is a cheerful mage (specializes in elemental magic), while Raigh is a cold and calculating shaman (specializes in dark magic).
    • Fire Emblem Fates features two sets, though they're actually non-identical in both cases:
      • Nohr natives Felicia and Flora are more adept at combat and housework, respectively. When push comes to shove, Felicia's loyalty is to Corrin, while Flora's loyalty is to her tribe.
      • From Hoshido, Kaze is fairly calm and collected, while Saizo is more Hot-Blooded and blunt. Kaze becomes one of Corrin's closest allies in all three routes, while Saizo distrusts Corrin in the two routes in which he joins. Fittingly, Saizo's hair is red and Kaze's is green.
  • Saku and Bo from .hack//G.U. are twins taking turns playing one character, Sakubo. You can tell who's logged on by the character's eyes (Saku has Tsurime Eyes, Bo has Tareme Eyes), hat (Saku has the "sun" side pointing up, Bo has the "moon" side), and their general attitude towards you.
  • The Get Backers game Jagan Fuuin has the Mikado twins: Daisuke is very compassionate and sweet and easily befriends the main cast, whereas his identical twin Kyosuke is cold, emotionless, rarely speaks, and seems robotic. It's eventually revealed that the twins are the same person, namely Kyosuke, who, due to fear of their immense spiritual power, were split into two entities, the "body" and "soul". Daisuke is the soul, and once they're merged back into Kyosuke, he acts much like his "brother" did.
  • Sven and Grancy from Gyee. Sven is Hot-Blooded, outgoing, and passionate person who has fire powers and is classified as a Warrior. Grancy is calm, stoic, Deadpan Snarker, and is a Mage who uses ice powers. Sven has black hair with a red streak, while Grancy has white hair with a blue streak.
  • Felix and Ted Huxley from Kindergarten 2. Felix is a pint-sized Rich Bastard who constantly belittles and bosses around his brother and is planning to have him murdered so he won't have to share his inheritance. Ted on the other hand is far more pleasant, but is also an Extreme Doormat to his brother and so desperate to win his and their father's approval that he's willing (though reluctant) to go along with Felix's plan to murder one of their classmates, even though he doesn't even know why said classmate needs to die beyond vague reasons of "inheritance" and "for the good of the company".
  • Mother 3: Lucas is shy and withdrawn, while Claus is rash and reckless.
  • The Neptunia series has the extrovert Ram and the shy Rom. These twins are also a huge source of irritation for their big sister, Blanc.
  • In Pathfinder: Kingmaker Kanerah and Kalikke are identical tiefling twins. Kalikke is idealistic and dreamy, Chaotic Good, controls water, and can heal others at a cost to herself. Kanerah is pragmatic and ambitious, Lawful Evil, controls fire, and empowers herself by torturously offloading the cost of her abilities onto dead souls. Due to peculiar magical circumstances, they're rendered unable to exist simultaneously, communicating only through notes, and one of them comments this has the benefit of preventing them from constantly fighting.
  • Andrey and Peter Stamatin from Pathologic and its sequel/remake are a classic Red Oni, Blue Oni. They're both architects who have worked together on many of the town's structures, but Andrei is a more social person who runs his own bar, is hedonistic, and has a somewhat hot temper; Peter, on the other hand, is depressive, tends to keep to himself, and gets drunk on the town's version of alcohol.
  • Yu and Rei, the ghost twins from Puyo Puyo. Yu is a loud, pun-spouting Genki Girl while Rei is quiet and depressed. It's implied their personalities were vice versa when they were alive.
  • The Ryō twins from Senran Kagura. Ryōbi is a sadist who hates her flat chest, whereas Ryōna is a masochist who loves her large rack.
  • The Sims 2:
    • In Strangetown there's Lola and Chloe, in the Singles household, although whether or not they are actually twins is somewhat debatable. Lola is Married to the Job, while Chloe is a Hard-Drinking Party Girl.
    • The Davis twins from the University expansion are described as "different as night and day" within the game, yet they actually have identical personalities and aspirations. The only real difference between them is their appearances and what they like to talk about. Similarly, the Pleasant twins in Pleasantview are supposedly very different but have very similar personalities.
  • Street Fighter: Hyperactive Yun and brooding Yang of the Lee Brothers.
  • The titular Super Mario Bros.. Mario is brave, outgoing, and Hot-Blooded; Luigi is reluctant, timid, and shy.
  • The Bruiser Brothers in Super Punch-Out!!. Rick Bruiser is outgoing and hot-blooded, while Nick Bruiser is quiet and cold.
  • The Yorigami sisters from Touhou Project; Jo'on is prideful and domineering while Shion is gloomy and pessimistic. Jo'on can steal other people's wealth and fortune for herself while Shion causes everyone around her to suffer misfortune, including herself.
  • Greg and Sidney Kasal from Trauma Center. Sidney is serious and straight-laced, while Greg is far more relaxed.
  • In Until Dawn, Beth is blunt, cautious, and responsible, compared to her twin sister Hannah, who is naive, sensitive, and impulsive.
  • Vampire: The Masquerade - Bloodlines: The Voerman sisters, stoic businesswoman Therese and emotional party-girl Jeanette. You eventually discover that this trope is subverted. Therese is a Malkavian, a member of a clan of vampires known for insanity, and her "sister" Jeanette is actually her Split Personality developed to cope up with her father's abuse that cranks up to eleven due of being a Malkavian means worsening one's existing mental health issues. With high enough Persuasion skill, you can convince the "sisters" to cooperate instead of one "killing" the other.
  • The Warcraft universe has Malfurion and Illidan Stormrage, the night elf twin brothers. The former leads his people to druidism as an alternative to the dangerous magic that brought the Burning Legion to Azeroth. The latter insists on tapping both that magic and the power of the demons themselves, is imprisoned for centuries, and eventually becomes an Evil Overlord.
  • Calvin and Sam from What Remains of Edith Finch. Calvin wants to be an astronaut, and Sam a soldier.
  • Xenoblade Chronicles 2: Pyra is a Rose-Haired Sweetie with a surprising strength. Mythra, her other personality, is a tough warrior girl who can be surprisingly tender. This was, in fact, intentional. Mythra was the original personality, and was so horrified when she destroyed three Titans (killing many of her friends in the process) that she created Pyra to be everything she wasn't. Pyra is even an excellent cook when Mythra is aggressively terrible.
  • Xenoblade Chronicles 3: Segiri (Number 7) is The Stoic who speaks exclusively in Robo Speak, but is ultimately an excellent leader who wants what is best for everyone. Number 13 is an Affably Evil Blood Knight Lone Wolf who doesn't care about anyone besides Segiri. For bonus points, no one even knows what twins are due to being born from Uterine Replicators; everyone is rather confused that the two look identical, and their opposing personalities just confuse them more.

    Visual Novels 
  • Dahlia Hawthorne and Iris from Phoenix Wright: Ace Attorney – Trials and Tribulations. Dahlia is a ruthless sociopath who has been using her cute and innocent image to get away with murder, literally, for years and Iris is sweet, gentle, and compassionate, and honestly fell for the lead character while posing as her sister because she needed him for a master plan but didn't want to be bothered with actually seducing him.
  • CLANNAD: Kyou and Ryou. Long-haired tsundere and short-haired, easily-embarrassed sweetheart.
  • Higurashi: When They Cry: Mion and Shion Sonozaki. Mion is assertive and bombastic, the leader of her True Companions; Shion is sly and seductive, preferring to manipulate people subtly rather than openly command them. It's even more odd when you realize that they're acting like each other, thanks to a Twin Switch they made when they were younger.
  • Tsukihime: Kohaku and Hisui. One is carefree, energetic, and always smiling, while the other is silent with little expression. It's also odd when you consider that they switched places from when they were children: Hisui was originally carefree and cheerful, while Kohaku was shy and untalkative. Then they got older and things got worse for them (specially Kohaku), so Hisui buried her emotions to help Kohaku stay stable while Kohaku hid her very darkened True Self behind a facade of happiness.
    • Averted in Flower of Thanatos side story, where Kohaku and Hisui synchronize to the point where they are no longer themselves and their personalities fuse into one. Becoming Single-Minded Twins instead.
  • Ember and Daystar in the ElfQuest visual novels. Ember is active, bold, and like her name in looks and attitude. Brother Daystar is mystical and calm, and blond with pale skin.
  • Little Busters! has Haruka and Kanata, who form an All Work vs. All Play duo. In reality, though, these roles were mainly either in response to or outright enforced by their abusive extended family and in the end when they are able to move on from their past and accept one another as friends their personalities become a lot less extreme. Not entirely, though.
  • ef - a fairy tale of the two.: The Shindou sisters. Chihiro is an ingenue, Kei is a Shorttank.
  • The Kazato twins from If My Heart Had Wings. Asa is the timid one, and Yoru is the blunt one. Their differences in personality are literally night and day.
  • Tokimeki Memorial 2 has the twin sisters Miho and Maho Shirayuki. Miho is a sweet, daydreaming girl who believes in fairies, loves fortune-telling and plushies, and dreams of becoming a scenario writer of stories for children. Maho is a playful, blunt, and mischievous street-wise girl, who enjoys fashion and window-shopping, and dreams of becoming a model.
  • The Prince of Ladykiller in a Bind is a largely asexual, highly educated, callous Manipulative Bastard. The Beast, his Half-Identical Twin sister - who has to take her brother's place for a week - is much more affable, extremely flirtatious, and pretty Book Dumb.
  • Super Danganronpa Another 2 has the Otonokoji sisters. Hibiki is brash, outspoken, and feisty, while Kanade is more submissive, friendly, and gentle-hearted. Additionally, Kanade proves to be quite helpful and insightful during Class Trials, while Hibiki isn't initially willing to trust anyone but gradually warms up to her classmates. Chapter 3 later reveals just how deep it runs: Hibiki is really a kind girl who genuinely loves and cares for her sister, while Kanade is revealed to be an utterly remorseless Yandere who's spent years making her "inferior" twin sister emotionally dependent on her. Namely by mentally abusing her and killing everyone who she feels gets too close to Hibiki.

    Web Animation 
  • MoniRobo: Mayuko and Mayuka are twins with opposite personalities. Mayuko is an active, sporty girl while Mayuka is a smart, quiet girl. They're still opposites even after their mother Akane divorced their father Yoshiichi and took Mayuka with her: Mayuko became more diligent on helping her dad with the chores and raising her grades, while Mayuka became a delinquent after her mom neglected her for so long.
  • In Red vs. Blue, North is a relatively Nice Guy who is more than willing to put himself in harm's way to save others, while South tends toward Chronic Backstabbing Disorder, with tragic results for her brother.

    Webcomics 
  • It's Walky!: Fraternal twins Beef and Walky. Justified as Beef was actually switched at birth with Walky's real twin Sal as part of Mrs. Walkerton's Separated at Birth gambit, Sal and Walky later, after the truth was uncovered, and used to set up some "Not So Different" Remark moments between the two of them.
    • Sal and Walky again in Dumbing of Age despite not being raised apart this time around.
  • LnM: L is energetic and childish, M is laid back and precocious.
  • Sikue and Tatsuma in Beyond Bloom, are not identical, but were "born" together. They are polar opposites in many ways, one being loud and aggressive, while the other is quiet and gentle. This is especially emphasized in their magic. Tatsuma has offensive abilities while Sikue's are defensive.
  • Gogo and Didi from Bomango. Gogo is a Boisterous Bruiser with a short fuse, a heterosexual Lad-ette, and a Jerk with a Heart of Gold who acts as The Id in their Freudian Trio (Andy is The Ego). Didi is a polite perky scientist and a Lipstick Lesbian who acts as The Super Ego. They're a weird example though, in that Didi slowly budded off Gogo which would make her some kind of clone/daughter, but they see each other as twins and have a more sisterly relationship.
  • Subverted in Cursed Princess Club. While Gwendolyn and her twin brother Jamie couldn't look more different from each other (she's a creepy goblin-like girl, he's a pink-haired Pretty Boy), they are both equally sweet, kind, and supportive.
  • Played with in Ennui GO!. Izzy and her twin sister Adelie are very similar, the only real difference being that Adelie lacks Izzy's crippling depression and prefers alcohol to drugs. Meanwhile, both of them stand in contrast to Izzy's clone Kirsty, who is a Genki Girl and lacks their cynical attitudes. This is justified by the cloning process didn't include Izzy's memories.
  • El Goonish Shive:
    • Elliot and his Opposite-Sex Clone Ellen are very different from each other. Elliot is reserved, shy, and is naïve to a ridiculous degree. Ellen is brash, worldly, and outgoing. Justified since Ellen has tried to distance herself from Elliot's memories, and she also had an entire lifetime's worth of experience from an Alternate Dimension implanted in her head.
    • Susan is a nerdy asexual introvert while Diane is a bisexual Lovable Alpha Bitch. Justified since they weren't raised together and aren't actually twins; they're distantly related through an immortal elf and only happen to be identical to each other due to destiny or magical shenanigans or elsewhat.
  • Molly and her clone/twin Galatea in The Inexplicable Adventures of Bob! were raised separately. Both are brilliant, naive, and devoted to Bob, Jean, and each other. But while Molly is upbeat, friendly, and gentle, Galatea is paranoid and megalomaniacal.
  • The Order of the Stick has Elan and Nale, who look the same except for a goatee on Nale, but their personalities are the complete opposite. Elan is good, naive, and Genre Savvy, while Nale is evil and scheming. It turns out that Elan got his goodness from his mother and his genre savviness from his father, while Nale got his evilness from his father and his Complexity Addiction from his mother. Their opposite is also indicated by their names.
  • Realta: The Gemini twins. Fletcher is cheerful and gregarious, while Milo is more serious.
  • The Hong twins in Sharp Zero. Elliot is dorky, unorganized, and believes others see him as the evil twin and a screw-up. Marcus is the put-together Mom Friend was a straight-A student, and never gets in trouble.
  • Sleepless Domain: Forte Bass (aka Gail) and Outrageous Apple. Bass has music-based powers, is pretty modest, and is mute, while Apple runs on a rebel/punk/fruit theme, uses her skateboard to attack, is loud and brash, and speaks enough for both of them. Oddly enough, they both still have red as their magical girl color.
  • Unsounded: The Gefendur gods are two pairs of very different twins. Riv is a violent macho manly man while his twin Baelar is a quiet warrior scholar and poet. Yerta is a plump, loving fertility godess who loves creating life while her twin Tirna is a skinny wrathful trickster who doesn't understand the appeal of imprefect living things.
  • Welcome to Room #305 has the Hot-Blooded ladette Yoona as the older twin to Yoon Sung, a (normally) stoic young man. As young children, Yoona was a tomboy while Yoon Sung was a sensitive crybaby. Both twins have tempers when they want to though.

    Web Original 
  • Amoridere:
    • Doki is much different than her twin sister Toki. Both dress somewhat alike but wear different colors: black or purple for Toki, reflecting her enigmatic, yet domineering but secretly kindhearted personality, and pink and white for Doki, reflecting her kind, gentle, and angelic personality. It isn't just that, according to Amoridere, Doki is more morally grounded than Toki is (as in, she wouldn't stoop to schemes or anything illegal to meet her demands, whereas the latter probably and more often than not would), along with being more levelheaded, and, Toki, well, she is described as being one who does what she wants, whenever she so wants to do it, consequences be damned. It was also noted that, when the two were children, Doki was always the shy one, while Toki was more assertive.
    • Brownie is very much different than her sister, Brittani, in that Brittani is described as being prim and proper, while Brownie is described as tomboyish. Apparently, them looking different than one another is supposed to reflect that.
    • Killerbunnies: Kamu and her twin sister Kanran are somewhat similar in some personality traits, however, Kamu can be said to be more levelheaded and is typically polite whenever needed, whereas Kanran is typically gluttonous, rude, and usually acts before thinking, making her rather foolish. Likewise, Miren and Citrine, with the former being described as being "Lean and Mean" and the latter being "bright and bubbly".

    Web Videos 

    Western Animation 
  • American Dragon: Jake Long: Oracle twins Kara and Sara (pictured). The gothic Kara foresees good events in the future and is always gloomy because everything good about to happen gets spoiled for her, while cheerful and preppy Sara foresees the bad events and is always so happy because any time the horrible things she sees aren't happening is absolutely fantastic in comparison.
  • The Angry Beavers: Dagget and Norbert, twin beavers, the latter of whom is sensible and collected and the former of whom is essentially insane.
  • In BoJack Horseman's Show Within a Show, Mr. Peanutbutter's House, the titular character adopts human twins Zoe and Zelda; the former is outgoing and cheerful, while the latter is sullen and brooding. It became an In-Universe meme in the show's fanbase to classify people as either "Zoes" or "Zeldas" depending on their outlook and personality.
  • The Cramp Twins: Lucien is smart and highly responsible, with a keen interest in nature; while Wayne is a mischievous prankster and bully who is scared of animals.
  • CatDog: Cat and Dog, Conjoined Twins who are essentially the opposite of each other. Cat is sophisticated, risk-avoidant, and short-tempered. Dog is uncultured, reckless, and chipper.
  • Pixar Shorts: Day & Night actually took this literally, featuring an encounter with two traditionally-animated figures, through which views of a CGI world during different times of the day can be seen.
  • Dragon Tales: Zak and Wheezie, who are even very different colors despite being conjoined. Zak is green and uptight, while Wheezie is purple and a Genki Girl.
  • ElfQuest: Suntop and Ember actually brought CBS down on them when their creators tried to get an Animated Adaptation going. Turns out Saturday Morning Cartoons is not the time to reverse gender roles and have a spunky little girl and a mild, intuitive little boy. Or, for that matter, parents with different skin colors.
  • The Fairly OddParents!: Timmy's more serious and responsible fairy godparent Wanda has a twin sister named Blonda who's a fun-loving TV star.
  • Freaky Stories features a story about a pair of twins who take advantage of their wildly different interests in talents by using Twin Switch to appear to be one perfect person.
  • Kim Possible: Gemini identifies himself as being opposite his twin sister Dr. Director in every possible way, which is mostly accurate. They don't even look much like siblings, let alone twins.
  • Kaeloo: Rabbit sisters Pretty and Eugly. Eugly is a kind, friendly Gonky Femme, and Pretty is a beautiful yet selfish and manipulative Alpha Bitch who is hated by the rest of the cast.
  • The Loud House has identical twin sisters Lana and Lola. Lana's masculine, tomboyish, and loves getting dirty, whereas Lola dresses like a princess, regularly competes in beauty pageants, and is far more conceited than her twin.
  • My Little Pony: Friendship Is Magic:
    • Pinkie Pie and her fraternal twin sister, Marble Pie. Pinkie Pie is bright pink, exuberant, and outgoing, while Marble Pie is a dull gray, reserved, and very shy.
    • According to the IDW Comics, the mild-mannered schoolteacher Cheerilee has an identical twin sister named Cherry Blossom who's a professional wrestler.
  • Muppet Babies: Scooter and Skeeter. The former is a socially awkward computer nerd, the latter an energetic tomboy who likes sports. They sang a song dedicated to this in the episode about opera, as seen in the quotes section.
  • Razzberry Jazzberry Jam:
    • The Drum Brothers, Buddy and Krupa, are about as different as night and day- a fairly apt metaphor, as Buddy is cheerful and sunny, while Krupa is gloomy (and snarky).
    • There’s Bonita and Woody from “A Bonnie Tale”. Bonita is stuffy, uptight, and dislikes improvisation, and while Woody is never actually seen, from the way the Jazzberries talk about him he’s cool, fun to hang around with, and has a tendency to make up stories about his supposed adventures (which Bonita hates).
  • The Interesting Twins From Beneath the Mountain from Codename: Kids Next Door come off as creepy, Single-Minded Twins at first, but after a series of failures, their personalities shine through: the female twin is hot-tempered, commanding, and impulsive, while her brother is calmer and more likely to go along with her plans (he is fiercely protective over her when she's upset, however). Plus, the birthmarks on their face make them a literal example of Sibling Yin-Yang.
  • The Venture Brothers: Thanks to Divergent Character Evolution - Dean has slowly become a nervous, cowardly Non-Action Guy while Hank has evolved into a Small Name, Big Ego who leaps into danger at the slightest provocation.
    • Also bitter, unsuccessful, frail Rusty and the much more skilled and charismatic JJ. It is implied that JJ got all the best DNA from their father, despite having spent decades as Rusty's conjoined foetal twin.
  • Gravity Falls:
  • Yin Yang Yo! has smart Neat Freak Yin and her less intelligent violence loving brother, Yang. No wonder their names are literally Yin and Yang.
  • Sofia the First: Prim and proper reserved Amber who is prone to flights of fancy with her down-to-earth practical casual funloving brother James.
  • Grojband has brothers Kin and Kon Kujira, who are a diminutive Gadgeteer Genius and a Fat Idiot respectively.
  • Downplayed on The Simpsons: Patty and Selma are similar in their chain-smoking, raspy voices and dislike of Homer; but Selma has married about a half-dozen times, while Patty initially seemed uninterested in romance. Since then, she's come out as a lesbian, though still isn't as quick to commit as Selma.
  • Miraculous Ladybug:
  • Ninjago: The Hands of Time are definitely this.
    • Kru is old because he has been waiting forty years for his twin. He hates modern technology but admits that it has its uses, and at least tolerates his brother's love for what he hates. His powers are Reverse and Pause, for which the colors are copper/yellow and red. He's the brains and the calmer of the two but can fight on his own, even without his powers.
    • Acronix is young, having traveled forty years into the future. He loves modern technology but is willing to return the old ways in life for Krux, though not without trying to get him to like technology. His powers are Forward and Slo-Mo, for which the colors are green and blue. He is the more excitable and brawny of the two but still able to understand technology within just a few days after his return. He's also quite adaptive, especially in battle.
    • In the past, their outfits are even inverted versions of each other. In the present, they've undergone different Costume Evolution. Krux's is darker and more faded in coloration (since it's forty years old), and has an hourglass with a chain printed on his left torso and right leg, but was already there when Acronix returned. Acronix's is brighter and more vibrant in coloration just like it was in the past (since it hasn't had forty years in which to fade) and later has a phone with green earbuds attached printed on his right torso hand left shoulder. Acronix's hair is long and black while Krux's is white (formally black) and short. Krux has a mustache while Acronix has a beard. Krux has a pocket watch that is rarely seen outside his disguise, while Acronix has a smart watch that is constantly seen on his wrist.
  • Strawberry Shortcake: Berry Bitty Adventures has Sweet and Sour Grapes. As their names indicate, Sweet is cheerful and friendly. Sour is crass and sometimes downright mean, particularly in situations where she's reminded that she and Sweet are twins. Both are bakers, but Sweet favors, well, sweet dishes, whereas Sour focuses on savory dishes. Lampshaded in their first appearance.
    Strawberry: They don't look much like twins.
    Lemon: I don't think they want to.
  • Total Drama: Amy and Samey are identical twins. Amy is an Attention Whore and a Manipulative Bitch, whereas Samey is an Extreme Doormat yet also The Heart of her team. Amy goes out of her way to make trouble for her sister and treats her like a servant, while Samey just wants to get out from under her sister's thumb and become more confident in herself.

    Real Life 
  • America's Next Top Model: Amanda and Michelle Babin, who were contestants on Cycle 7. Michelle is tomboyish and more outgoing, whereas Amanda is more feminine and introverted. Michelle also came out of the closet during the course of the show, just to add another night and day difference to the mix.
  • Nikki and Brie, the Bella Twins of Total Divas and WWE fame. Brie is a Granola Girl, who loves being a hippie and Country Mouse - but also is a Hard-Drinking Party Girl. Nikki is The Fashionista and powerlifter, who loves anything expensive - while preferring wine and desserts to excessive drinking. Their workout methods are different, with Brie opting for yoga and cardio while Nikki enjoys powerlifting to maintain Amazonian Beauty.
  • Tokio Hotel: Bill and Tom Kaulitz are identical twins, but (among other things) they dress and wear their hair differently, to the point that some people don't believe they're identical. One of the more notable differences is Bill's focus on "true love," while (until recently) Tom was only looking for love of the one-night variety.
  • The public perception of the Kray twins has traditionally had Ronnie, the younger twin, as pure Ax-Crazy psychopath, and Reggie as quieter and more Affably Evil, even acting as something of a Morality Chain to his brother. There's not necessarily much evidence of this, but that just makes the fact that people want it to be the case an example of this trope in action.
  • Twin musicians Chuck (bass) and John (drums) Panozzo of Styx. Chuck is soft-spoken and more down-to-earth, while John was a wild man and more outgoing.
  • Happens to twins in Real Life, as twins growing up together try to develop different personalities to distinguish themselves from their sibling. However this is usually subverted in identical twins — twins that came from the same zygote, not from two different zygotes in the womb — as they are practically genetically identical copies of each other and will share many similarities in personality and preferences even if they act differently. National Geographic has done an article on it.

 
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Alternative Title(s): Different As Day And Night, Different As Night And Day

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Gru's Twin

Gru discovers that he has a twin brother named Dru. And when he meets him, he is clearly different from him.

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