Affably Evil: Barry the Chopper. He's fine with joking around with his name, and answering Ed's question; tying Ed up is as hostile to him as he gets. He even asks if they still can't be friends, despite wanting to chop him up. Predictably, they find common ground, as a butcher and nightmare fetishist, respectively, which they have a heart-to-heart talk about.
Frankly, he's nicer to Ed than the military.
Lampshaded by Ed, after he and Winry are freed.
"Nice bloke, though, if you look past all the mutilation, I mean."
Alchemy Is Magic: Lampshaded to hell. Ed's insistent on it being called "magic" instead of alchemy and being a "magician" than an alchemist.
Annoying Younger Sibling: How Ed feels about Al, he only brought him back because Al couldn't be his servant if he wasn't there.
Approval Of God: PurpleEyesWTF, the guy he's imitating, appears in an episode.
Big Brother Bully: Both Ed and Russell. Alphonse and Fletcher bond over the various abuses and shenanigans that their respective siblings put them through.
The Caligula: King Bradley. Creates a mandatory national holiday specifically to make fun of Mexicans, and later grants Edward an alchemy license just for sitting in a chair.
Decreed the death penalty for anyone wearing green in what is widely remembered as the worst St. Patrick's Day ever.
Cloud Cuckoolander: Many, and in tiers, no less. Bradley sits at the top, followed closely by Maes Hughes and Barry. Roy Mustang is a few rungs beneath, and Ed only a little ways below that. Only Riza and Al qualify as sane.
Death by Adaptation: Psiren, instead of being encased in the giant hand Edward summons up like in the anime, Ed instead crushes her to death, much to Al's dismay.
Foreshadowing: Shou Tucker isn't the least bit subtle about his plans for Nina. He later even lampshades how obvious the buildup was.
Surely Faurlerro's using his native British accent for Envy was a random stylistic choice! Even though he's quite the convincing Fake American for all his other non-Elric voices.
Rose: Hey, has anyone ever told you you sound like Johnny Yong Bosch?
Al: I don't like Johnny Yong Bosch.
MasakoX as Trisha Elric
purpleeyeswtf as Barry The Chopper
Megami33 as Lyra
Faulerro has confirmed on Tumblr that MasakoX and Megami33 are likely coming back as Sloth and Dante, respectively - though if you've seen the anime you probably saw this coming.
Winry: Ahh, would you feel that refrigeration unit doing it's thing? ...This is divine! I just want to be inside it!
Lampshaded a few lines later by Barry.
Barry: Now, I know what you're thinking: my truck is pretty hot, but you're wrong! It's cold as crap in there. See, that guy's gonna freeze his balls off.
Last Request: On her deathbed from lazy malaria, Ed's mother asked him to not call it alchemy as it sounded pretentious.
Leaning on the Fourth Wall: Ed's lengthy gushing about Barry's "mutilations." Barry is guest-voiced by PurpleEyesWTF, creator of the abridged series-es that inspired this one.
As of Episode 7, Ed as admitted that the grotesque fascinates him. Al outright calls him a nightmare fetishist.
Only Sane Man: Both Al and Riza have found they relate to each other.
Occasionally Edward, when he's not threatening to eat Father Cornello or going in psychotic rants.
Roy Mustang - at least, in comparison to Fuhrer Bradley and Maes Hughes.
Police Are Useless: Oh yes. Not only do they confuse a 12-year-old with an adult woman twice his height, but they're utterly baffled by the use of magic. In a setting where said magic is basically public knowledge. What.
Shout Out: Al's Johnny Yong Bosch voice and his response - "I don't like Johnny Yong Bosch" - are both references to one of the anniversary videos done by LittleKuriboh. In the video, LK had a puppet that spoke in that same voice - claiming to be JYB - and he would always say "I don't like [thing]".
The final paragraph in King Bradley's long explanation of Edward's title (episode seven) is the lyrics to the theme song of The Fresh Prince of Bel-Air. note
"...this is the story all about h..." and "...to take a m..." can be seen.
In Episode 2, Ed insists that Cornello's ring is the Sorcerer's Stone, not the Philosopher's Stone. He then says "Now give me my stone," which is a reference to a particularly narmy line from Alone In The Dark
Tempting Fate: In episode 3, after Winry tells the Elric brothers that her parents are dead. Ed responds that something like that will never happen to their mother (while saying ever a few times). 3 seconds later, she's hit by lazy malaria and dies.
Title Drop: "Nullmetal Alchemist" becomes Ed's official title.
Roy: A Nullmetal Alchemist, yes! What do you think?
Ed: That... doesn't make any sense.
Roy: Ha ha! Of course it doesn't! These things aren't meant to. "Ment", get it?