Emily Dupont: Hold on... Your partner? Or your... "partner"?
Morris: Yes, yes, lesbian cops. No one has ever heard that joke before.
Dupont: It's the haircut, dear.
- Bleach: Depending on your interpretation of her character, Suifeng may qualify as this; given her position as head of the Keigun (punishment squad) and her Ho Yay obsession with Yoruichi. Mind you, this is more apparent in the anime than it is in the manga, but still...
- The sheriff in the second volume of Dogby Walks Alone.
- A Certain Scientific Railgun: The Judgement doesn't have de jure police authority, but Shirai Kuroko is an ESP-powered example of this trope. And she's actually a competent cop too, when she's not being (Played for Laughs) Psycho Lesbian.
- Captain Maggie Sawyer of the Metropolis Special Crimes Unit in Superman comics. Introduced in 1987, her sexual orientation was established less than a year later.
- Detective Renee Montoya of the Gotham City Police Department was a Batman supporting character for many years before being outed in Gotham Central.
- A Psycho Lesbian cop appears in The Punisher. She first appeared in the Garth Ennis run as Soap's new partner, and later returned (her Psycho Lesbian side was mostly explored in the arc with Elite's son).
- Sgt. Jackie "Jack Phantom" Kowalski from Top 10. In the later Beyond the Farthest Precinct mini-series, she is in a relationship with her partner Panthalassa.
- Lucille, Marv's probation officer from Sin City.
- Margaret from Slither.
- Amy Wallace (Laurie Metcalf) in Internal Affairs
- Commissaire Laurence in the belgian film Dikkenek. There is also another female cop who tries to hit on her, but fails.
- Detective Sergeant Stephanopoulis in the Rivers of London books, described in text as looking like "a woman who fought rottweilers" and "a terrifying lesbian." She's actually a very nice person, and an utter professional in the Old-Fashioned Copper mould.
- Kate "Casey" Martinelli, the protagonist of a series of mystery novels by Laurie King.
- Kate Delafield, hero of a series of detective novels by Katherine V Forrest beginning with Amateur City.
- Detective Calliope Skouros in Otherland. This becomes slightly important when Dread decides she wouldn't be much fun to rape.
- Eve Dallas from the In Death series gets called this occasionally. She's not; it's pretty much shorthand for showing what an asshole someone is. She is very married, and all Les Yay comments between her and Peabody are strictly for Rule of Funny.
- Detective Monica Jansson in The Long Earth is mentioned to be lesbian several times, usually by her superior officer. It is treated in a very offhanded manner and does not relate to the plot much otherwise.
- Sergeant Sue Smith in Halting State by Charles Stross.
- On Law & Order: Special Victims Unit, when Olivia asks a female cop if she'd been having an affair with her (male) partner, the cop looks Olivia up and down and replies,
Kristin: Let me make this very clear, Detective: I'd be much more inclined to have an affair with you.
Olivia: Do you ever get a gay vibe from me?Elliot:...Would it matter if I did?Olivia: You're not answering the question.
- Olivia herself is constantly subject to Les Yay speculation by fans (lesbian fans, anyway), usually with a relationship with Alex in mind and much pining for the shorter, butcher haircut and leather jacket of the early seasons. It was probably partly in response to this impression that the show made obvious attempts to make her more feminine as the series went on — longer hair, more makeup, nicer clothes, a more "motherly" attitude toward victims and more references to/depictions of her having a boyfriend.
- She is also Mistaken for Gay by a lesbian activist who cites Liv's job and attitude as indicative of being a lesbian. Later, Hilarity Ensues (and lampshades are hung when Liv asks Elliott if he gets a gay vibe from her and he points out that she doesn't have much luck with guys.
- Agent Carrie Rivai in the 2008 Knight Rider remake.
- Agent Burke's probie in the first episode of White Collar is implied to be one. It's confirmed when she returns in season 2, she has a girlfriend that she met while working in DC and who moved with her back to New York.
Neal Caffrey: [pouting after finding out] Doesn't the FBI have a policy about that?
Peter Burke: That's the military. We don't ask. We don't care.
- Kima Greggs of The Wire.
- Third Watch raced ahead of this trope in its first episode. Boscorelli, masculine and Irish was accused of being gay as part of a prank. While driving around after who he thought was the prankster, he complained to his partner and asked what she'd think if people thought she was a lesbian. She answered "I'm a female cop, people assume I'm a lesbian."
- On NYPD Blue Medevoy donated sperm to a lesbian cop and her girlfriend so they could have a baby.
- DC Jo Masters from The Bill.
- Roseanne: Roseanne's sister Jackie was a police officer, but while she wasn't a lesbian in the show, Roseanne reveals in the final episode that the series was a book she was writing based on her family and that her sister actually is a lesbian.
- On Lip Service, essentially BBC's answer to The L Word, Sam is a soft butch example of this trope.
- Mo Connell from Between The Lines is introduced as a lesbian, although it later turns out, in reverse of the normal pattern, that she's Bi the Way.
- Detective Grayson, a Recurring Character on Leverage.
- Downplayed with Charlie Buckton from Home and Away, who had one brief lesbian affair. There's also a more unfortunate example in an earlier storyline, when it was revealed that Psycho Lesbian Zoe McAllister had a lover on the force.
- Nadine Lemercier in the French series P.J. (Police Judiciaire).
- Stef in The Fosters.
- Tara Chalmers in The Walking Dead although technically not a cop, she was only in the academy when the ZA hit.
- Parodied in a 2014 episode of Saturday Night Live, where a Buddy Cop Show from The Seventies apparently starred a character named Dyke and was played by Les Dykawitz. Her female partner wasn't gay, however.
- Goodness Gracious Me's "Back Where They Came From" special featured several sketches about a duo of female traffic cops. The last sketch at the end of the show reveals that they are in a relationship.
- Detective Erica Richards from Eternal Knights initially "subtly" expressed lesbian feelings for Kathryn "Artemis" Kennedy; after helping to rescue Kathryn from an illegal interrogation, they engaged in lesbian sex.
- Detective Rebecca Morris in Sam and Fuzzy was initially implied and later confirmed to be lesbian, as was her partner Sarah Cooper. Morris confirms later they didn't date because of Unequal Pairing, which was 'fixed' when Cooper got Laser-Guided Amnesia and had to quit the force.
- On 3Way, the show within a show "Ladycops" plays with this trope.
- Lesbian Cops takes this Up to Eleven: They're both lesbians, and they're so in love with each other that they'll boink on the job.
- Maggie Sawyer in Superman: The Animated Series has the same sexual orientation as her comicbook counterpart, according to Word of God, although the closest they could get to referencing Maggie being gay on-screen was to have her girlfriend be at her bedside when she was in hospital during the first Darkseid story. She goes unnamed, and their relationship isn't elaborated on, but she's there. She's also present, comforting Maggie, after Dan Turpin dies.