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- The eponymous Index of A Certain Magical Index: a girl with perfect memory who memorized 103,000 grimoires and therefore has an almost complete knowledge of all magic, including the forbidden stuff. She has some kind of immunity to the usual mind destroying effects reading those grimoires has, which is why the organization she belongs to had her read them: easy access to all the knowledge they wanted. Unusually for the trope, she's a 14 year old girl rather than some ancient creature.
- Boom! Studios' Fall of Cthulhu story introduces the Harlot, an obscene Eldritch horror who parodies a burlesque madame. She offers incredible and unspeakable knowledge, but in return the student becomes her property kept inside a wicker box. The Harlot is depicted as a rival and opponent of Nyarlathotep.
- Nyarlathotep from H.P. Lovecraft's Cthulhu Mythos is characterized like this in most of his original appearances. Both in "The Haunter of the Dark" and "The Dreams in the Witch House" he is a gatekeeper to secrets beyond human ken, and must be appeased with human sacrifices in order to relinquish some of this knowledge, and in The Dream-Quest of Unknown Kadath he protects the Gods of the Earth from any mortal molestation. In his original appearance in the prose-poem "Nyarlathotep" he instead grants humanity information in abundance as a figure of science, leading to the entire world Going Mad From The Revelation.
- The Doctor Who New Adventures novels have the Library of St John the Beheaded, which collects and stores forbidden texts (both in the sense of banned-by-the-mundane-authorities and in the sense of Knowledge For Which The World Is Not Ready). It's definitely got the isolated or hazardous location part of the trope down pat; on Earth, it was hidden away in The City Narrows, and in The Future it will be located on an asteroid as part of the Braxiatel Collection, which stores texts for which many worlds are not ready. In principle, it's an aversion of the usual some-things-man-should-not-know corollary: the founder believed that all knowledge is useful if handled carefully, and potential researchers are vetted very carefully before being offered access to the collection. (The Doctor, of course, holds the first ticket the Library ever issued.) In practice, though, pretty much every time it's appeared in a story it's because somebody's found a way to use information from the Library to cause trouble.
- Zeus Is Dead: A Monstrously Inconvenient Adventure has The Moirae of classical mythology (a.k.a. The Fates), though they're not evil so much as they are very, very detached. And strict. Apollo manages to get some information out of them, but only at the cost of his own skill with prophecy.
- Tren Krom in BIONICLE
- Several players in Destroy the Godmodder have claimed to be or actually are this.
- The_Serpent is/was the only player with knowledge of the psi-godmodder artifacts.
- In Dungeons & Dragons, the ancient demon named Dagon (a reference to H.P. Lovecraft) lives within the Abyss, but holds a vast array of information due to his age and intellect, to the point he's become the shadow ruler behind the Prince of Demons' (Demogorgon) entire war stratagem and survival.
- Orabilis, the End of All Wisdom, in Exalted, a demon lord who spends his time building libraries, and killing demons who learn things they're not supposed to.
- In The Elder Scrolls series, this is within the sphere of Hermaeus Mora, the Daedric Prince of Knowledge. Mora specializes in forbidden knowledge, and is the only Daedric Prince who doesn't bother a humanoid form when dealing with mortals, instead preferring a truly Eldritch Abomination form of a mass of eyes, tentacles, and claws. Mora himself claims to be one of the oldest Daedric Princes, a Time Abyss class of deities, forming out of detritus concepts ejected from reality. Mora's preferred method of seducing mortal servants is to bribe them with gifts of power and knowledge. He also tends to give them absolute freedom, trusting that the lure of the gifts he offers will keep them in his service. His realm, Apocrypha, an endless library containing all knowledge in the form of tomes and very much an Eldritch Location. The sky is an illuminating green in color and it is covered by a sea of roiling acidic waters. Some areas of Apocrypha are consumed by a darkness which kills any mortal who tries to enter it. The realm is haunted by the ghosts of mortals forever searching for knowledge, while it is maintained by Mora's servants, the Seekers and Lurkers. Mora's most famous associated artifact is the Oghma Infinium, a Great Big Book of Everything bound in Genuine Human Hide. Those who read it gain immense knowledge and power from it, but it tends to disappear before the reader can read too much.
- In League of Legends Nasus holds knowledge of the magic of life and death, although he does teach it to people who are judged worthy by his brother Renekton. The number of people who weren't worthy and wanted the power eventually drove Renekton insane and he started slaughtering first them, then everyone.
- The Pillar Of Skulls in Planescape: Torment is one.
- killer7: Christopher Mills knows that Garcian's the main personality, not Harman, and also knows that the government is controlled by the Secretary of Education. This gets him killed.
- The Secret World has a fairly benign example in the form of the mysterious half-magic half-mechanical Bees; native to the Hollow Earth, they not only grant the player character his/her magical powers at the start of the game, they also provide nuggets of ancient knowledge that they've been collecting over the eons in the form of the lore entries you track down. Of course, given that the only way anyone can access the Bees' "education protocol" is by actually being touched by the bees, the main price for all this forbidden knowledge involves having your entire life turned upside-down in much the same way the player character's was. The other price is actually having to track down each fragment of lore.
- The Keepers are one of the three factions in the mythology of the Thief series and represent Neutrality between the chaos of the Pagans and the order of the Hammerites/Mechanists. They are an ancient order of scholars who have been recording all knowledge of the mystrious runes, that seem to have a great power over the city, but also include agents who are masters of stealth and spying. But as the third game reveals, even they have their own dirty secrets which are no less dark than those of the other two groups.
- In Fallout: New Vegas, in the Good or Neutral Karma ending of Old World Blues, the Courier becomes the new guardian of the Big Empty, releasing technology to help the people of the Mojave Wasteland on an as-needed basis.
- In Star Wars: The Old Republic, Darth Thanaton is the member of the Dark Council who controls the Sphere of Ancient Knowledge. That is, until the Sith Inquisitor defeats him and inherits his place on the Council.
- In Star Control 3, the Captain somewhat becomes this. After learning the True Name of the Eternal Ones, he can share this knowledge with the Daktaklakpak, causing them to self-destruct.
- Video Game/Stellaris has an entire faction of these, two if you buy the expansions. The first are the Keepers of Knowledge, an ancient and decrepit empire of scholars and hoarders who will occasionally reward you if you send them scientists (consenting or otherwise) and punish you if you research certain proscribed tech. The others are the Curator Enclaves, and they are far more friendly. They actively help you destroy pan-galactic threats and monsters, they send you scholars to help your research if you make the effort to befriend them, and offer a colossal 1/5th increase in research speed if you pay them a tithe.
- In Sarab, the Hubs are the only places science may be learned and they are very exclusive.
- Avatar: The Last Airbender brings us Wan Shi Tong, He Who Knows Ten-Thousand Things, a spirit in the form of a giant owl who seeks to collect all information in the universe. He once had it all in display in a library for all to see, but eventually realised how humans used this information to hurt and kill others, and decided all knowledge should be forbidden.
- Parodied in The Grim Adventures of Billy & Mandy a giant worm girl was forced to guard the "secrets of the universe". She then lured hundreds of kids into finding it, the secret was there was no secret. When Mindy found it, she now has to take her place in guarding the secret, while the worm is free.