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- Naruto: In Shippuuden movie 6, Naruto's evil counterpart turns out to have black hair. The other counterparts, who are not evil, have the same hair as the originals. However after the real culprit, Tobi, is defeated, Naruto's counterpart becomes blond and probably good again, since he was under Tobi's control.
- Raven-haired Queen Nehelenia is the Evil Twin of Queen Serenity in the original Sailor Moon manga. Fanon often attributes them to be sisters, but this is unconfirmed. Their connection is written out in the anime adaptation.
- In Queen Millennia, Selene is the darker-haired sister of the protagonist and was the antagonist for the first half of the show. Though this is subverted as the rest of the cast eventually switch sides to her point of view.
- Inverted in Pandora Hearts. The black-haired Alice is the heroic twin to the deranged Will of the Abyss, who has white hair. It's actually more complicated than that, as both sisters are good — the White-haired Alice is malicious only because she shattered her own memories to save her sister and Oz, and has been reduced to a Psychopathic Manchild state. When her mind is intact, she's gentle and kind-hearted in comparison to Alice's more boisterous nature.
- The original Betty and Veronica once parodied this trope.◊ Considering that they are essentially blonde/brunette versions of each other, some Archie stories employ this trope. Despite popular belief, whether Betty or Veronica is the good or bad one, or if there even is a good or bad one, varies by the story; it is very telling that they are wearing the reverse costumes in the aforementioned picture.
- Power Girl once fought an evil black-haired clone of herself named "Divine".
- Smurfette has one among the Grey Smurfs in The Smurfs story "The Smurf Menace".
Film - Animated
- Vanessa for Ariel, Disney's The Little Mermaid, is a magically induced trope example of this trope. They actually look like each other, but Vanessa's constant scowling makes this less obvious.
- Ironically, Ursula was originally meant to have been Ariel's aunt, so it is tempting to think Ursula may have actually resembled Ariel in her youth.
- Most adaptations of The Little Mermaid make use of this trope, with the temple princess being a dark-haired version of the titular mermaid, though whether the princess is a villain or an innocent player varies by adaption.
- Daria's cousin Hildegarde from The Princess and the Pea.
Live Action TV
- Cousin Serena on Bewitched is arguably the Trope Maker. Though whether she was mischievous or truly wicked depended on the episode, Serena was antagonistic, and often indulged in pretending to be Samantha.
- Section-Leader Elizabeth Shaw, the Mirror Universe version of Dr Liz Shaw in the Doctor Who story "Inferno", had a brunette bob, in contrast with the regular Liz's shoulder-length blonde hair.
- Gilligan's Island had an episode "All About Eva", where a woman named Eva, who resembles a nerdy black-haired version of Ginger, comes to the island. The castaways befriend her and make her over, leading her to resemble Ginger. In the end of the episode, she not only abandons the castaways to return home, but steals Ginger's life!
- Pictured above: Jeannie's Sister (who was also named Jeannie) from I Dream of Jeannie, obviously inspired by Serena, is one of the more well-known examples of this trope. Like Serena, her most common scheme was pretending to be the good Jeannie. Despite popular belief, while sisters, they were not literally twins, though the show went back and forth on which one was younger.
- Inverted on Orphan Black, where Sarah is a brunette and her evil (at first) twin Helena is blonde. The inversion extends to the other main clones: Cosima and Alison are brunettes, and antagonist Rachel is blonde. Krystal (good and blonde) breaks the pattern, possibly because it's overridden by another trope.
- Inverted once with The Patty Duke Show, where Patty and Cathy are revealed to have a third lookalike, yet fair-haired, cousin named Betsy (who was of course Duke in a blonde wig). While somewhat sympathetic, Betsy spent the episode scheming to convince Cathy to return home so she could take her place. She also stole Cathy's date early in the episode.
- Narrowly inverted with Vicki and Vanessa on Small Wonder. According to Word of God, Vanessa was originally going to wear a blonde wig, but it was dropped when it was realized her main shtick was posing as Vicki anyway.
- Mariah Carey used this trope in her "Heartbreaker" video. The evil lookalike, Bianca, tried to steal Mariah's boyfriend in the video. While the implication is that the boyfriend was cheating on Mariah, it is tempting to think that it was just a case of Mistaken Identity.
- Parodied, among other things, in this MadTv skit
- Nicki Minaj made use of this in her "Va Va Voom" video, in a parody of Sleeping Beauty, playing a red-haired princess and a black-haired witch.
- Taylor Swift made use of this in her video for "You Belong With Me," contrasting the blond Cinderella-esque heroine contending with a brunet Alpha Bitch.
- Avril Lavigne did a very interesting case in her infamous "Girlfriend" video. A black haired bully tries to steal a red-haired lookalike's boyfriend, and ultimately wins. Word of God is that the black haired girl is evil and the red-haired girl is good, making the former a Villain Protagonist and the later is a Hero Antagonist. Make of that what you will. Added to that, Lavigne also plays a blonde narrator of sorts.
- In a rare male example, Chris Martin used this in Coldplay's video for "Magic". He not only played the hero of the video, but also the Stage Magician mixed with Dastardly Whiplash villain of the video.
- Magical Girl Lyrical Nanoha A's Portable : The Battle of Aces has The Stoic dark brown-haired Material-S/Stern the Destructor and the blue-haired Blood Knight Material-L/Levi the Slasher, the Evil Twins of the light brown-haired Nanoha and the blonde Fate, though they've gone through a Heel–Face Turn by the The Gears of Destiny sequel.
- Inverted in Phoenix Wright: Ace Attorney where it's the brunette twin Iris who's the benevolent one and the red-haired Dahlia who's a total Bitch in Sheep's Clothing.
- Deadly Premonition has an example that isn't quite inverted or played straight. The two Francis Morgan personae, York and Zach, Zach being the "real" one, are differentiated by York's black hair and Zach's white hair. Neither can be said to be evil, though.
- In Adventures of the Gummi Bears recurrent villain Duke Igthron is actually this of his twin blonde brother Sir Victor, the exact opposite of Igthorn, Sir Victor is a heroic noble and faithful knight. Another episode has a French Jerk princess who is like the evil version of Princess Calla, but brunette (although she gets better in a latter episode).
- Cousin Sunny for Gwen on Ben 10: Ultimate Alien. Sunny's human form was later shown to be an illusion, so it is unclear if she intentionally made herself resemble Gwen or if it was her default form.
- Inverted with Ben and Albedo from the same series.
- Krystella and Jycella from Chaotic., literally play this straight, but, unusually, the villainous Krystella is the one introduced first, and her nicer twin is introduced later.
- Word of God confirmed that had The Grim Adventures of Billy & Mandy spin-off "Underfist" been successful, Eris would have obtained an evil black-haired twin (to compensate for her love of Hoss making the original Eris good). While the two never made a proper appearance in the "Underfist" special, both Good Eris and Bad Eris can be seen in the end credits.
- Cousin Damina on The New Archie/Sabrina Hour was a raven-haired version of the good witch Sabrina. Unlike most examples, Damina never got the chance to exploit their resemblance.
- Starfire and Blackfire on Teen Titans, though this does not apply to their comic book versions. They also have different eyes, though this didn't stop Blackfire from trying to steal Starfire's identity.
- While their resemblance may be limited to the series' art-style, there is also Kim & Shego in Kim Possible.
- Gigi Grant, a genie from Monster High, has one in her Evil Twin, Whisp. Considering they're genies, it is possibly a shout-out to I Dream of Jeannie.
- Wendy the Good Little Witch had one in her cousin, Wildcat.