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- The titular Astro Boy. He was created by Dr. Umataro Tenma as a Replacement Goldfish for his lost son. However, he abandoned Astro who was adopted by Professor Ochanomizu. Astro also had a sister name Uran who is either created by Ochanomizu or by Tenma. There was also a brother name Cobalt. Astro even had robot parents created by Tenma in an attempt to atone for abandoning Astro.
- In HeartCatch Pretty Cure!, Professor Sabaaku created Dark Pretty Cure and treated her like an actual daughter. Then it was revealed that Sabaaku was actually Yuri Tsukikage's father, making Dark Pretty Cure her sister.
- Kill la Kill Nui Harime is a Life Fiber Artificial Human created by Ragyo Kiryuin, who Nui considers her mother. With everything it entitles. She also sees Ryuko as a "blood sister" because they both have Life Fibers in them.
- Lyrical Nanoha:
- In Magical Girl Lyrical Nanoha, Fate Testarosssa is actually a clone created by Precia Testarossa base on her deceased daughter Alicia. However, because Fate ended up being entirely different from Alicia, Precia becomes an Abusive Parent. Despite that, Fate still loved her mother and considers Alicia her older sister.
- A two mommy variation with Reinforce Zwei from Magical Girl Lyrical Nanoha A's. She was created by Hayate Yagami by using the left over Unison Device of Reinforce Eins. She's often treated as the baby of the Yagami family even if she says she is an adult.
- Magical Girl Lyrical Nanoha StrikerS:
- The Numbers, 12 cyborgs created by Doctor Jail Scaglieti. He regards all of them as his own daughters which some do share his DNA and they in turn see him as a loving father. Even the ones who turned face. There's also a small example of Parental Incest since he implanted clone embryos of himself in the sisters incase his plans were to fail.
- Subaru and Ginga Nakajima, the adopted daughters of Genya and Quint, are revealed to be cyborgs themselves whose DNA are base on Quint. This also meant that they were sisters to The Numbers even before some of them were adopted into the Nakajima family.
- Subverted with Vivio Takamachi, the adopted daughter of both Nanoha and Fate. At first glance, she would seem to be their biological kid since she looks very similar to them. She really just a clone of a long ago ruler and has zero blood relation to Nanoha and Fate.
- Rei Ayanami from Neon Genesis Evangelion is a clone created by Gendo Ikari via combining the DNA of Lilith and his late wife Yui. Gendo is shown to have some affection for her. Though, it might be because she looks just like his wife. There's been debates on whether Shinji's attraction to her can be consider incestual since she shares his mother's DNA.
- Re:CREATORS deals with fictional creations of entertainment entering the real world and meeting their creators. Most of the interaction between the creator and their creation are akin to parent and child.
- Takashi Matsubara (Creator) and Celesia Upitiria (Creation). The two started out rough as they often argue with each other. Others who see this compare it to a father arguing with their daughter. Later on they do developed a much deeper and caring relationship.
- Military Uniform Princess aka Altair (Creation) and Yuna Shimazaki (Creator). Altair hates the real world and wants to destroy it because she blames it for causing Yuna to commit suicide.
- Humorously done with Nishio Onishi (Creator) and Hikayu Hoshikawa (Creation). Others are creep out that Nishio wants to marry Hikayu even though she is basically his daughter. He doesn't really care.
- In Dr. Slump, Bungling Inventor Senbei Norimaki lives with his robot daughter Arale. He tells the other villagers that Arale is his little sister, despite being old enough to be her father.
- The main Universe of Tenchi Muyo! has Mad Scientist Washu being the mother of Space Pirate Ryoko. She did that by combining her egg cells with a sentient material called Masu which Mihoshi actually referred to as Ryoko's dad. Theres also the cabbit Ryo-Ohki who was also made out of Masu and is thus Ryoko's sister.
- The DCU
- Kon-El's Superboy is a clone combining the DNA of Superman and Lex Luthor. While Supes never treated Kon as a son, he does see him as part of his family along with his adopted parents and his cousin. Lex, however, is treated as Kon's Archnemesis Dad as he tries to persuade the Boy of Steel to his side.
- Wonder Woman has Princess Diana whose original origin story has her being sculptured from clay by Queen Hippolyta and given life by the Greek Gods. There's also Donna Troy who in one of her many origin stories has her being the magical mirror clone of Diana to act as a friend.
- Marvel Universe
- In Avengers: The Initiative, the Scarlet Spiders are all clones of Michael Van Patrick mixed with DNA from Baron von Blitzschlaug. He frequently refers to them as his boys.
- The ever Tangled Family Tree that started with Hank Pym and his rebellious robot son, Ultron who has an Oedipus Complex on Pym. He once attempted to create a wife by using the brainwaves base on Hank's wife Janet and naming her Jocasta. Then there is Ultron's sons Vision and Victor Mancha. Vision himself marries Scarlet Witch and with the use of her Chaos Magic, created two sons for themselves. And later on, Vision creates a Robo Family consisting of a wife, a son, a daughter, and a dog. And the wife's brainwaves are base on Scarlet Witch's. And then there was the time Hank Pym was in a relationship with Jocasta which squicked others out since he is technically her grandfather. Though, she sees it as being with God.
- Laura Kinney a.k.a. X-23 is the Opposite-Sex Clone of Wolverine, created by The Facility. How their relationship was seen actually change from that of a brother-sister dynamic to a much more father-daughter one once Logan decided to adopt her and Draken saw her as a sister. There's also Dr. Sarah Kinney who was forced by Dr. Zander Rice to give birth to X-23. While sympathetic, Sarah was emotionally abusive to X even if she did try to help her as much as she could. Eventually she did pull a Heel–Face Turn and tried to save Laura from the Facility. Laura also saw Sarah's sister and niece as her aunt and cousin even if there was no true blood ties. Rice himself can be seen as the "father" to X-23 as he was the head scientist to the project. He was portrayed as the true abusive parent since he focus all his hate on Wolverine to her. It is theoretically possible that Laura may have actual Kinney DNA, given her slight resemblance to her mother and the fact that the DNA sample used to create her was damaged, which may have required the splicing of Sarah's DNA to repair it.
Films — Live-Action
- Played with in A.I.: Artificial Intelligence. Henry and Monica Swinton are employees of Cybertronics, the company that manufactured Robot Kid David. David was intended as a Replacement Goldfish for the couple's comatose son Martin, but after some initial tension Monica grows to care for him.
- In Attack of the Clones, Jango Fett's DNA was use for the basis of the Clone Army. He asked for one unaltered clone (i.e. no accelerated aging) that he raised as his own son. That son being Boba Fett.
- Godzilla: Biollante started life as just a simple rose crossed with the DNA of Genshiro Shiragami's deceased daughter in an attempt to resurrect her. Later on, Biollente was dosed with Godzilla's mutated cells, transforming her into a kaiju that's part plant, part human, and part Godzilla. While the relationship was never clearified, most labelled Biollante as a "sister" to Godzilla.
- Like in the comics, Laura a.k.a. X-23 is created by using Wolverine's DNA. Even if others refer to her as his daughter, Logan doesn't want to have anything to do with her. However, he has a change of heart doing the course of the movie and Laura does call him dad as he lays dying and feels pride in those words.
- Even though the moments are brief, there's the relationship between Zander Rice and X-24, a clone of Logan. Rice actually comforts X-24 as he injects the healing serum like a father would to their child. Then there's X-24 mad reaction of seeing Rice dead.
- Saving Mr. Banks: P.L. Travers is very reluctant to give Walt Disney the movie rights to her Mary Poppins books, as she has a personal connection to her characters. Disney understands this, as he feels the same way about his most famous creation.
- Subverted in Splice, where Clive and Elsa increasingly strive to dehumanize Dren even as it becomes clear that she is sapient.
- In Toys, the youngest member of the Zevo family, Alsatia, is a Cloud Cuckoo Lander who works in the family toy company's dolls division. She's revealed to be a robot after being accidentally shot by one of the older brother's experimental weapons.
- Saber of Red is Mordred, the "son" of King Arthur and Morgan le Fay. However, instead being born naturally she's really a homunculus clone created when Morgan stole sperm from Artorianote and given birth from Morgan's womb.
- Berserker of Black is actually a genderflipped Frankenstein's Monster. Her backstory is more or less the same with one major difference being that Victor Frankenstein is much more of a Abusive Parent then in the book.
- In The Adventures of Pinocchio (as well as the adaptation Pinocchio by Disney), the elderly woodsmith Gepetto creates a sentient puppet named Pinocchio which he treats as his son.
- In Dark Lord of Derkholm, the wizard Derk's specialty is creating magical hybrids, like pigs with wings. His masterworks are the griffins, built from combinations of avian and feline DNA with a bit of human DNA for intelligence — they have human-level minds, and he regards them as just as much his children as the ones he fathered by more traditional means.
- The titular Monster from Frankenstein and his father, Victor Frankenstein. The doctor created the Monster out of human corpses and gave it life. However, after seeing how hideous it was, Victor attempted to destroy it. Feeling betrayed by its father, it goes on a rampage and ruins Frankenstein's life. But despite all that, the Monster still shows sorrow on the Doctor's death.
- October Daye: on paper, May Daye is Toby's twin. May is a personified death omen who didn't wink out of existence when Toby's death was averted. This is a played with example, as Faerie itself created May, not Toby.
- In Captain Vorpatril's Alliance the Jewels were both the creation and the children of Udine. Her husband Shiv treats them as his children too, and their (more or less) natural children consider them siblings. Although they all do make a distinction between the "odd sibs" and the "even sibs."
- Eve: Shortly after being brought home by the Clarkes, Eve is identified as "Will's American cousin", although her role is more like that of a sister/daughter.
- I Am Frankie: Frankie is seen as a daughter by Gaines. Frankie in turn sees her family as her own.
- Kamen Rider Ex-Aid
- Burgermon, the Bugster from Ju Ju Burger, was created by Tsukuru Kobashi. Instead of being frighten by the Bugster, he was happy that Burgermon was real and treated him like a son.
- Poppy, another Bugster, actually sees Dan Kuroto as her birth father since he created her and named her. The feeling is surprisingly mutual as Kuroto also sees her as family. Might also be because Poppy is the Busgster of his mother.
- Downplayed with Parado and Emu. While no true family connection was made, since Parado did came out of Emu's body and wanted to play with him and the two ending up as Bash Brothers, the two can be seen as brothers.
- K.C. Undercover: KC refers to Judy as her younger sister, identifying herself as the big sister.
- Astronaut Molly Woods of Extant is married to inventor John Woods, who has created a Robot Kid called Ethan to be their son because Molly is infertile. John cares for Ethan deeply, and gets distressed when people question Ethan's "humanity" or "lack of a soul."
- In Star Trek: The Next Generation, Data (an android) calls his creator "Father", his creator's wife "Mother", and another android created by his creator, Lore, who's evil, his "brother". In the episode "The Offspring", Data made a child android girl named Lal who he called his "daughter".
- Small Wonder: This was the central premise of the series. Ted Lawson built a robot girl named Vicki and he, his wife and their son all had to pretend she was a member of their family and not a robot.
- The Twilight Zone (1959): In the episode "The Lateness of the Hour", a scientist's daughter discovers that she is actually a robot he built. This knowledge drives her mad and he ends up reprogramming her as a maid.
- In Fate/Grand Order
- Downplayed with Mash Kyrielight who is a designer baby created to host the Heroic Spirit Galahad, the son of Lancelot. Even though Lancelot had zero involvement in Mash creation, he still sees her as his daughter.
- Jeanne d'Arc Alter is an interesting case. She was created by Gilles de Rais with the use of the Holy Grail and molded as a twisted vision of the regular Jeanne d'Arc. When made into a playable Servant, the event has her trying to make a harem with Gilles playing the father archetype. Regular Jeanne also sees her as a little sister and thinks her retaliation on her to be cute.
- The ninja Assassin Kato Danzo is a robotic karakuri doll created by the Mage Koji Kashin and empowered by the first Kotarou Fuuma. She view both of them as her parents. Danzo also acted as the "mother" for the other Fuuma. Kotarou Fuuma the Fifth, the one that the protagonist can summon, outright calls her "Haha-ue (Honored Mother)".
- Hephaestus in the God of War Series created Pandora as an Artificial Human to serve as the key to Pandora's Box, but eventually comes to see her as his daughter, a fact which earns him much mockery and scorn from the other Olympian Gods, who refuse to see her as anything more than a pale imitation of real humans. Kratos similarly becomes obsessed with Pandora as a Replacement Goldfish for his dead daughter Calliope, and, like Hephaestus, is mocked by Zeus for this.
- Using Astro Boy as one of their inspirations, the main story of Mega Man is about Dr. Light creating the first sentient robots to help humanity, where his first models (Proto Man, Rock/Mega Man and Roll) are considered as his own family and helping him in home labours, especially Rock, being treated almost like it was his son.
- Downplayed with Samus Aran from Metroid. She was born natural by human parents but was genetically altered by her adopted family the Chozo who also gave her their DNA. In the Manga, Mother Brain, who was created by the Chozo, called herself Samus' "mother" since not only did she help in Samus' upbringing, she was the one who injected Samus of the Chozo DNA. Though, her jealousy over Samus being the true legacy of the Chozo race can be compare to sibling's rivalry.
- Doctor Klim from Virtue's Last Reward clones himself to create a successor to finish his work if he dies before it's finished, and raises the clone (very poorly) as his son. He also intended for a robot he built to act as his son's mother-figure, but his clone rejected her and saw her only as a machine.
- In El Goonish Shive, a magical mishap creates Ellen, an Opposite-Sex Clone of Elliot. Elliot and his parents adopt her into the household almost immediately and she quickly comes to be seen as his de facto twin sister.
Ellen: Really. The spontaneous creation of a female clone with all your memories. That was simple.
Elliot: "She's family. Help her." Not that complicated.
- Molly the Peanut Butter Monster in The Inexplicable Adventures of Bob! considers Bob and Jean her parents (which they are to a certain extent), even calling them Mommy and Daddy even though she was fully grown in body and mind long ago.
- Adventure Time:
- The entire Candy Kingdom was created by Princess Bubblegum and she sees them as her children who she cares about. Even people like Lemongrab who is an annoyance is still loved by her.
- Goliad and Stormo were created by Princess Bubblegum with the former created by standard candy parts and Bubblegum's baby teeth and the latter created with Finn's DNA. Goliad refers to Stormo to as his brother and Finn himself refers to Stormo as his son.
- "Bonnibel Bubblegum" reveals the first three Candy People were explicitly meant to be Princess Bubblegum's family. She was still (physically) a child then, so she made them older than she was—specifically an uncle, aunt, and older cousin modeled on a family she found a photograph of.
- NEPTR, a machine created by Finn to act as a pie thrower. NEPTR actually considers Finn as his father. Unfortunately, Finn is at worst a neglectful parent as he easily forgets he even exist.
- There's Dani Phantom from Danny Phantom, the Opposite-Sex Clone of Danny created by Vlad Plasmus. She did referred to Plasmus as her father but stopped after finding out he was just using her. And while she did referred to herself as Danny's cousin, she saw him as a brother after saving her. Had the series continued, Dani would of been adopted into the Fenton family
- The clone gargoyle Thailog had three creator fathers. Goliath is his DNA template, Dr. Anton Sevarius is the one who created him, and David Xanatos gave him his education. While Goliath was enrage at him, Elisa points out he is more or less his son. Though, Thailog attempted to kill him and his other fathers after finding them a disappointment. He also has an Oedipus Complex with Goliath as he hit on Elisa Maza, Goliath's Love Interest, enter a relationship with Goliath's ex, Demona and created Delilah, a clone combining the DNA of both Demona and Elisa.
- Anton Sevarius creates a new giant gargoyle by using the DNA of the entire Manhatten Clan. He actually saw it as his own son as he named him Little Anton and was sadden when he permanently turned to stone.
- Justice League's Galatea was a clone of Supergirl created by Dr. Emil Hamilton for Cadmus. Despite Emil's hatred against Supers, he actually love Galatea like a daughter and she love him like a father.
- My Life as a Teenage Robot has XJ-9 aka Jenny Wakeman created by Dr. Nora Wakeman. While the latter expects the former to act as a defender of the Earth, she still sees her as her own daughter and cares for her as such. There's also XJ-1 through 8 who Jenny sees as her younger sister but who Nora doesn't have that close of a relationship.
- Like in the book, Pinocchio is a wooden marionette created by Gepetto and who he treats like his own son. The Blue Fairy can also be seen as Pinocchio's mother since she brought him to life.
- The Powerpuff Girls
- Professor Utonium created the Powerpuff Girls and acts like their father in every way. And while the Girls always refer to him as the Professor, they love him like a father.
- There's Bunny, the temporary fourth sister of the PPGs. She was created by the girls after feeling so exhausted from saving the day.
- There's Mojo Jojo and Rowdyruff Boys who were created very similarly to the Girls. The four villains are shown to have some family affection. The Boys would be later resurrected by Him with the use of magic, making him their other father. Once Mojo found out, he wasn't too happy and battle Him in a literal Custody Battle for the Boys.
- In Samurai Jack, the Daughters of Aku are literally the Daughters of Aku. They were conceived when The High Priestess of the Cult of Aku drank some of Aku's dark essence.
- In "H is for Haunted", Will creates an Astral Drop, magical clones with no personality, to do her laundry. Big Bad Nerissa then turns it into an altermere, an artificial being with actual feelings and emotions, and convince her to take over real Will's life. After the two Wills battle, the original one realized the other was an actual being and the two made peace. The two then referred to each other as sisters.
- Nerissa creates a altermere of Yan Lin since she knew she be unable to convince the real one to join her. After defeating Nerissa, the altermere Yan Lin is introduce as Mira, Yan Lin's long lost sister.
- X-Men: Evolution was the first appearance of Wolverine's Opposite-Sex Clone, X-23. During the two's battle, Wolverine commented on that the two were the only family they had. What kind of family relationship was never established but many fans assume it would evolve into a father/daughter one.