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Films — Animation
- Ursula the sea witch, the main antagonist in The Little Mermaid, has Pat Carroll's throaty alto, opposing the more crisp and pure higher range of Ariel, the protagonist. The contrast is most clear at the end of Ursula's "Poor Unfortunate Souls", when she commands Ariel to sing. (This is reduced in the stage version, where Ursula sings in more of a mezzo register.)
- Mother Gothel, witch and main antagonist in Disney's Tangled, is voiced by Donna Murphy.
- Eartha Kitt provides the voice of the villainous Yzma in The Emperor's New Groove.
- My Little Pony: Equestria Girls (2013): Sunset Shimmer in has an alto voice that gets even deeper after going One-Winged Angel. , although Sunset retains her alto voice after having switched to the good side.
- The Dazzlings in the sequel, Rainbow Rocks, especially Adagio Dazzle, though Sonata Dusk averts this.
Films — Live-Action
- Averted in Across the Universe, in which the female lead has a mezzo-soprano voice that borders on alto, as do most of the women.
- Winifred "Winnie" Sanderson, the leader of the Sanderson sisters and the main antagonist in Hocus Pocus, played by Bette Midler.
- Rock of Ages : Both Catherine Zeta-Jones and Mary J. Blige play antagonists to the main heroes, and sing in a lower range than the heroine.
- Glee: The more antagonistic roles are played by the actors with lower voices, including Quinn and Santana (altos in the group) and Sue Sylvester. Kurt's voice is also in a much higher range than his antagonists.
- mothy being mothy, there are a lot of characters who fulfill this role in the Evillious Chronicles depending on which Vocaloid he uses. Banica Conchita/Master of the Graveyard (Meiko); Irina (Iroha); The Master of the Hellish Yard (Gumi); and Kayo and MA (Luka) are the most obvious examples, but there are numerous aversions: The Master of the Court, Riliane, Arte, Margarita, and Eve all have very high voices, being played by Miku and Rin.
- Mrs. Meers from Thoroughly Modern Millie. The owner of the Hotel Priscilla, she runs the business as a front for her real stock in trade; white slavery.
- Lucy from 13. Lucy is the Alpha Bitch and has learned how to manipulate her friends.
- Madame Morrible from Wicked. Though the wizard is the one initially responsible for the oppression in Oz, Madame Morrible pushes him to greater levels of tyranny and summons the storm that brings Dorthy's house to Oz. Then again, she barely has a singing part.
- Mrs. Lovett in Sweeney Todd: The Demon Barber of Fleet Street. She goes into business with Sweeney Todd, baking pies.
- Audrey II from Little Shop of Horrors. Though voiced by a man, she does refer to herself as a mother. And she eats people.
- Shenzi the hyena in the stage adaptation of The Lion King.
- Somewhat surprisingly, opera tends to avert this trope more often than not, partially because the pitch of the voice is perhaps more commonly correlated to a character's age than to their moral alignment, and also because sopranos tend to hog most of the bigger female roles. Some of the most notable evil or borderline evil female roles - the Queen of the Night from The Magic Flute, Lady Macbeth from Verdi's Macbeth, Puccini's Turandot, Richard Strauss's Salome, Alban Berg's Lulu, and Katerina from Shostakovich's Lady Macbeth of Mzensk - thus are soprano parts. However, Bizet's femme fatale Carmen was originally scored for a mezzo (like Delilah from Saint-Saëns's Samson and Delilah), but has also often been performed as an alto (or a soprano) and thus usually has a deeper voice than her foil, the angelic Micaela, and Herodias from Salome is a mezzo or alto. Klytemnestra from Strauss's Elektra is nominally a mezzo-soprano, but with much darker inflections than the soprano title character.
- In The Love of the Three Oranges Prokofiev averts it with Fata Morgana (soprano), plays it straight with Princess Clarisse (alto), and turns it up to eleven with the cook (a bass in drag). He also scores Hélène as an alto in his operatic adaptation of War and Peace.
- Played straight in most of Gilbert and Sullivan's operettas with the contralto (female tenor): Ruth in The Pirates of Penzance, Katisha in The Mikado... but averted by H.M.S. Pinafore with the unambiguously good Little Buttercup and in The Yeomen of the Guard with Dame Carruthers.
- In Matilda: The Musical, the evil headmistress Trunchbull has traditionally been a Crosscast Role played by baritone-tenors.