Bad-Guy Bar: The Machiavelli Club, though it's more of an upscale restaurant than a bar or club.
Big Bad: The Gentleman, much to Doc Ock's annoyance.
Big Damn Heroes: SAFE arrives just in time to rescue Spider-Man and Pity from frigid North Atlantic waters after their plane crashes.
Brainwashed: Pity underwent years of treatment and became The Gentleman's obedient servant/assassin. She does nothing without him first ordering her to.
Call to Adventure: Spider-Man initially doesn't want to get involved with the suicide of Brick Johnson. Until Mysterio crashes the funeral, endangers everyone present and announces that he will be going after the film industry.
Crossover: A rather odd one at the end of the third book Pity winds up picked up by Mystery Inc of all people as she's recovering from the climax.
Dark Action Girl: Pity plays this one straight, being able to go toe-to-toe with Spider-Man himself.
Disproportionate Retribution: When Quentin Beck and Brick Johnson were both young in Hollywood trying to make names for themselves, Brick refused to help Quentin get a job. Later after becoming Mysterio, he spends a year driving Brick insane with hallucinations, ruining his career and sending him into a state of suicidal depression and seeming insanity. Then he crashes the funeral and tries to kill everyone present.
Even Evil Has Standards: The Six with the exception of Octavius are disgusted and horrified by the Gentlemen's treatment of Pity, particularly the Vulture and Electro.
Evil Laugh: Mysterio is fond of these to the point that by the second book, Spidey suspects them being played from a tape.
Evil Old Folks: The Gentleman, who is orchestrating the whole evil plan, is said to be at least well into his 80s.
Karmic Death: The Gentleman who always put wealth above anything else, is killed by The Chameleon and has all of his fortune stolen from him. To rub salt in the wound, a detective whose life The Gentleman ruined confronts him as he lays dying. He thinks he has the final laugh until the detective points out he'll die a pauper. The detective then lays a penny on the opposite end of the room and leaves knowing The Gentleman will crawl for it.
Kick the Dog: Anytime discussion of the Gentleman's past comes up it's just an excuse for him to do this with a smile.
Large Ham: DON'T PRESUME TO FORGET OCTAVIUS, YOU FOOL!
Lightning Bruiser: Electro naturally but surprisingly Mysterio as well having extensive training as a martial arts stuntman.
Oh Crap: The Chameleon, after betraying the Gentleman and taking his fortune when Doc Ock comes on board his escape plane.
Remember When You Blew Up a Sun?: Early in book 3, a minor villain named the Disk Jockey gets into a fight with Spider-man. He takes a hostage and Spider-man tells him about the 6 people trying to kill him, some of the most deadly people alive. He mentions that he's fought all of them and in their most recent Boss Rush against him, he sent them all running by mid-afternoon to get the guy to back down. Doubles as a recap.
Sinister Silhouettes: The Machiavelli Club is designed so that everyone sits in the shadows and can't be overheard by others nearby.
Smug Snake: The Gentleman who is not nearly as intelligent as he thinks he is.
The Sociopath: The Gentleman, his brother Karl, and all the members of the Sinister Six, most notably Dr. Octopus.
Suddenly Sexuality: Mysterio gets lines like "I'm not, by life preference, as concientiously solicitous towards the ladies as Adrian", and describes the gay 1930s film director James Whale as an inspiration "both personally and professionally".
Tailor-Made Prison: In the beginning Electro is locked up in a plastic box suspended in water.