He's now come up with another. "Murder in a Minute."
Alcohol-Induced Idiocy: Drunk Minecraft. The only conclusion where you get a group of drunk adults playing Minecraft with an admin that's happy to give them whatever they want, provided they don't piss the admin off, which of course they do.
Badass Boast: Markiplier will make ridiculous boasts while playing horror games, usually to the effect of him being "too tough" or "so handsome" that the monsters are avoiding him, only to have one crash in and scare him into screaming bloody murder.
In part six of his Eleusis Let's Play he brags about how he could take on a wolf.
Badass Mustache: The signature of Mark's Alter Ego Acting, Wilford Warfstache, is a giant pink mustache. It has now become something of an official Markiplier emblem. Sometimes he even wears it while recording!
Bad Bad Acting: Mark's attempt at hiding his knowledge of the fact that "Error #53" is a horror game and not a cutesy adventure game is... Unconvincing. Especially noticeable as unlike his other videos he doesn't react to the scares at all, and blames all the monsters and jumpscares on Techno Babble terms.
Octodad's gameplay. He liked the game but damn did he rage over it.
Markiplier (on Catlateral Damage): Hope you enjoyed that. Was it good for you? It was good for me. Because we won this little shindig, and now we know, that there's more to life, than screaming at a stupid fuckin' octopus, that doesn't know what he's doing, and WON'T EVER COOPERATE WITH YOU, AND WON'T EVER DO WHAT YOU S-
"All right, kiddies!", for whenever he's imitating Santa Clause.
He also has been recently calling some monsters like the Ao Oni, "Butt-stabbers".
"That's what she said." Said whenever he makes an innuendo.
In Outlast, he's taken to saying "Little piggy no no, little piggy go go home now" whenever he goes through a narrow squeeze, thanks to the big burly guy calling him a "little pig" before tossing him off a two floor ledge.
"Eff you in the Ay, and have a nice day!"
He's recently taken to asking "Did I won? Diiid I woooon?" when endings are unclear, not even providing A Winner Is You screens.
Cluster F-Bomb: Markiplier typically makes a conscious effort to use PG language (saying such things as "hecky-heck" or bleeping his own language), even while playing video games that qualify as an M-rating, though will start swearing (sometimes A LOT) when he gets too frustrated with glitches in the games, his inability to overcome obstacles within them, or just generally freaked out.
His Let's Play of Dreadhalls is notable for possibly having the highest F-Bomb count in any Markiplier video to date.
One part of his Surgeon Simulator 2013 Let's Play had him so furious that he stopped playing for a moment just to let out a series of cusses.
"I have to get all the fucks out."
On his second playthrough of Vapour, he dies very many times causing him to rage. In the end, he gives us an amazing series of f-bombs, while Flipping the bird with two fingers.
Markiplier: God fuck me! God fuck it, fuck-fuck it, I dont want it! Fuck you, and fuck all of you, fuck you to hell, FUCK OOOOOFFFF!
Companion Cube: In the Amnesia custom story A Late Night Drink, Mark finds a tiny version of the normal wooden box and grows fond of it, calling it Tiny Box Tim. He gets separated from it later on, much to his sorrow, and when he finally crosses paths with the tiny box once again near the end of the story, he's unable to pick it up.
Cool Chair: Makes frequent use of and references to these during Amnesia: The Dark Descent and its subsequent Mods as a useless weapon/security blanket against the unkillable monsters featured in the games.
Markiplier: I have a chair! Yeah! You didn't like it last time! You'll like it even less this time. 'Cuz it's goin up yo butt.Whole chair, up yo butt!
Deadpan Snarker: Common to most Let's Play videos, Markiplier is humorously underwhelmed by the lack of logic that motivates the storyline, making aside comments to diffuse the tension and point out cliches in games.
Do Not Taunt Cthulhu: He practically waves his hands and dances in front of SCP-096, then barricades himself behind three doors to see if it gives chase. Yep.
Mark:(wiggles eyebrows) ............Is he coming after me? YEAH HE'S COMIN AFTER ME! AAAAGGGH (ripped to shreds)
Drinking Game: An interesting example is the Drunk Minecraft series, where Mark and his two real life friends Bob and Wade drink alcohol while playing Minecraft. It's more awesome than it sounds.
For a drinking game within a drinking game, take a shot every time a reference is made to The Walking Dead.
For his YouTube channel in general: take a shot for every video that has the words "scariest game ever" (or a variation thereof) in the title.
Epic Fail: A lot of his Happy Wheels highlights highlight these moments, especially the assassin levels where he somehow manages to kill himself a bunch of times, but never the target.
Gushing About Shows You Like: When he likes a game he is playing, he doesn't hesitate to compliment it. Even explaining his reasons why he likes it.
Hell Is That Noise: invoked The scraping stone feet of SCP-173. Mark invariably runs headlong into it, not grasping that 173 can navigate vents.
SCP-173's music cue whenever he's in the vicinity. Somewhere.
"WHAT? WHY IS THERE IS THERE MUSIC?!"
Heroic BSOD: When going through his first let's play of Slender: The Arrival, Markiplier was foiled by the proxy in chapter 3 just as he was about to escape via the mine-shaft elevator, sending him into a screaming mess. The next time, he manages to get to the elevator and up the shaft before this can happen again, and everything seems okay with the level effectively over. Then a bug in clipping issues when stepping off the elevator platform towards the exit sends the player character plummeting to their death. Mark is so utterly shocked and unprepared for what is quite possibly the most epic unintentional "screw you" destiny could ever give a gamer, that his commentary stops in it's tracks, as does his ability to even use facial expressions as he stares blankly at the screen for the next fifteen to twenty seconds in absolute silence, un-moving.
His playthrough of Mad Father featured a puzzle where he had to recreate a murder scene. He used the mini-chainsaw on the "terribly frightened" girl in the puzzle room, causing the girl to explode into bloody pieces. Mark sat, unmoving, with his mouth hanging open for a good five seconds.
Hilarity Ensues: Every. Single. Drunk Minecraft. Especially when they have a serious objective in mind.
This one in particular. They try to build Smokey the Bear. It turns out as... uh... just watch it.
Wade: Why do we try to build things? They NEVER turn out right!
Hoist by His Own Petard: During Mark's playthrough of A Late Night Drink, Mark spends 15-20 minutes searching for 4 keys in a library. He pulled out books for 20 whole minutes. One key was behind a candlestick NEXT to some books, but other than that, Mark needlessly pulled out books for 20 whole minutes.
What makes this even worse was the fact that Mark failed to recognize this as his own failure.
Lets See You Do Better: During his birthday stream he was playing Surgeon Simulator 2013's new mode (Meet The Medic), and after finally beating it, Wade was laughing in the background at Mark taking as long as he did and almost failing. Que an angry Mark making Wade play until he either won or failed, while he was sitting in the background with a rather funny (but angry) expression.
Misplaced Nationalism: In his Ampu-Tea video, Mark satirized the negative stereotypes that the British have of Americans—for example, claiming "Britain is a whole country! I know this!" Unfortunately, British viewers took Mark at face value and reacted accordingly. Many even used his antics as an excuse to spew hateful comments—including labeling all of America as Eagleland Flavor #2.
My God, What Have I Done?: Mark had a heartbreaking case after playing ''Ripest of Fears", a fangame dedicated to him. He realized that he's been trying so hard to get more subscribers that he's been neglecting the fans he already has. He promised to go back to how he used to be, and was desperately holding back tears at the end of the video.
He has an in-game example for Mad Father when he was setting up the final murder scene in the memory section. He playfully swung his mini chainsaw at the female statue only to successfully kill her. Obviously, he didn't realize this was part of the game and was just messing around.
Another in-game example is when he played Calm Time. He admitted that he just finished Drunk Minceraft and wasn't prepared for such a dark game. He especially felt bad for being forced to kill the only kid there.
The Nicknamer: Markiplier will, on occasion, randomly nickname characters in video games things like "Billy" (that's the name given to SCP-173 as well as to the Scavenger Bots in Dead Space 3) if he doesn't trust the character (as seen in his play-through of Penumbra and Nightmare House 2 as well as a sewer pipe he called "Jerry" in an Amnesia Mod.
This led to him stopping occasionally to apologize to his neighbors when recording from a hotel room.
Nothing Is Scarier: Markiplier often praises game developers for making use of this and gets easily freaked out from a games suspense and atmosphere when this trope is successfully implemented.
His collaboration with Yamimash on the Abandoned Metro Garry's Mod map shows that he has little tolerance for games and maps which seem like they're building suspense and atmosphere, but then don't do anything with it.
Properly Paranoid: Because he has played so much Amnesia, he has become terrified of mannequins. Even if they're inanimate, he freaks out and takes off their heads.
Drunk Minecraft in general tends to produce these. Some gems include the aforementioned waterfall doesn't touch the penis, "I'm being molested by an albino spider", and "Get your head out of my thong."
Running Gag: There's a few others that occur throughout most of his videos:
Poof! :3 (Mostly in the compilations, but it still counts.)
Making "Your mom"-jokes aimed at Wade. Actually, "hating" Wade in general has become a running gag.
Sanity Slippage: After a tough time in the final boss fight of Dead Space 2, he goes Laughing Mad and fills his inventory with nothing but Pulse Rifle ammo and health kits, and it's a little bit like watching The Joker do a Let's Play. He OWNS.
His entire Surgeon Simulator 2013video is an 11-minutes plus epitome of this trope. Just watch it.
Throughout the first play in the final video of Vanish, Mark becomes increasingly frazzled and panicked.
The Scream: Becoming cornered or trapped by SCP-173. In one playthrough, SCP-173 hops through an office window rather then use the door as expected...
Mark: No! NO! NOOOOO—AAAAAUUURRGHG(neck snap)
And in another, Mark faces it down as his eye meter depletes to zero...
Quite a few videos, particularly Drunk Minecrafts, have him screaming in fury as well.
Shout-Out: Mark occasionally quotes stand-up comedian Brian Regan in his videos. He also uses "Alright, alright, alriiiight" from comedian Kevin Hart.
In Happy Wheels Highlights #25 Mark says "Mycaruba," a catchphrase/running gag that was started by the Game Grumps (Mark is friends with the members of the GG channel). He also once asked "Barry" to edit his video while in-game.
During a play-through of Phobia 1.5, he uses a fast-forward cut while singing "Escape From the City" from Sonic Adventure 2.
More so when he completes the game Reunion. He finally reaches the boy he had been trying to find, to find out that the character he had been controlling was trying to kill the boy due to him suffering from schizophrenia. Mark is in complete shock from that point until the end of the video .
More humorously, when he finds the "Dancing Room" easter egg in The Darkest Words all he can do is stare.
Survival Mantra: "Little piggy no no, little piggy go home", starting with his first encounter with Chris Walker in Outlast, he sings this little verse in an attempt to ward off another incident of the man pulling him out of his hiding places. It even transfers over to Amnesia: A Machine for Pigs when he is running from the manpigs.
Usually doesn't do these and if he has a problem with a game he is usually direct with expressing it. Still does this occasionally, such as in Part 2 of his The Curse of Blackwater playthrough, when he gets attacked by a Necromorph, he comments that there must be a portal in the game to the Dead Space universe and mocks that it's where all the scariness of the series went.
There's also this little bit from Probably Archery:
Tempting Fate: Poor Mark just can't win in Containment Breach. He was sure that he'd eluded 173, only to open a sliding door and see the statue leering at him. Mark had just blinked before opening the door.
In another instance, Mark fled SCP-106 by ducking into an elevator. 106 simply walked through the wall.
In his Cry of Fear LP, Mark laughs off a warning telling him to 'watch out for the trees'. Guess what happens next..