The famous National Lampoon cover with the headline "If You Don't Buy This Magazine, We'll Kill This Dog" and a photo of a dog with a gun held to its head.
In the webseries and novel versions of Noob, one of Roxana's earliest story-relevant moves was to kill Sparadrap's entire pet collection.
Neil Young lets you know just how evil the FBI agents are in Greendale. When they break into the Greens' home looking for "evidence" after Sun's arrest, Sun's kitten scratches one of the men, who promptly shoots it dead and leaves it at the foot of Sun's bed.
The would-be-governor villain of the season from thisMark Trail storyline decided to cement his evilness by kicking the proverbial pet deer.
Little Orphan Annie: While it wasn't the first sign that she was no good, the fact that Trixie Tinkle kicked Sandy established that she didn't actually like Annie.
Mighty Man then uses his Phase to dispose of two of Hypnosís henchmen who are bent on causing the puppy harm.
The goblins of the Pathfinder RPG often come off as insanely clownish, what with their singing and antics and pyromania. Some players might forget that they're hateful and sadistic as well, until they demonstrate their absolute glee in killing horses and domestic dogs, which goblins consider to be mortal enemies.
In the gamemaster's guide, a section on determining the game's tone suggests taking care with potentially sensitive topics like sexual slavery, drug pushing, or violence against children and animals - some players might find their inclusion in a game to be tasteless, but they can be used to make a villain truly worthy of a righteous beatdown. The illustration on the page is a goblin standing triumphantly over the bloody body of a small dog.
In Assassins, Sarah Jane Moore shoots her dog for barking, then stuffs the dead dog in her purse — but it's Played for Laughs. As far as marking her as a credible threat goes, Sarah Jane's real Kick the Dog moment is when she turns her gun on her infant son, because he wanted an ice-cream. Thankfully, she doesn't pull the trigger.
In "A Very Potter Sequel", Umbridge is telling Harry that Sirius is going to be sentenced to the Dementor's Kiss, which kills the victim in Starkid's interpretation. She then happily says "and maybe he can say hi to your parents!"
Lucius doesn't even have to be present to do this. In a letter to his father, Draco says that Lucius can feel free to write back at any time, even though he hasn't written a single letter all year.
Lucius also takes the drawing Draco made (that includes a picture of him in the background proudly saying "That's my son!") and crumples it up, before throwing it in Draco's face.
In Me and My Dick, instead of comforting Joey after his failed proposal, Joey's Heart calls him fat and ugly.
Twisted: Ja'far's pregnant wife is taken away from him by the Sultan. Before he can do anything to get her back, she dies. Ja'far is also hated by everybody in the kingdom and everything that goes wrong is promptly blamed on him.
In Twice Charmed, Anastasia and Drizella torment Cinderella as Franco prepares to shrink her.
The Piraka in BIONICLE would occasionally kill animals for fun. Chiara, supposedly one of the good guys, had a scene where she casually killed a lizard with her electricity powers just to make a point. Because of this trope, many fans assumed it was foreshadowing a darker side to her personality, but Word of God states that this is not the case.
Appears on Homestar Runner, in Teen Girl Squad Issue 11. So-and-So is getting chewed out by her obnoxious manager at Shirt Folding Store when the manager is suddenly punched out by an astronaut ("MEET A FIST!"). The explanation for this behavior?
Astronaut: *ckhk* She killed my dog.
So-and-So: Um... 'kay.
Also referenced in the Strong Bad Email rated, where Strong Bad claims that some of his favorite movies have been banned in Transylvania, "where you're required by law to eat puppies for breakfast."
Strong Bad is also known to kick The Cheat, even though he's not really a bad guy.
In another Strong Bad Email, for kids, on his kids' show, Strong Bad invites children to play "Where's The Cheat?" with him:
Strong Bad: All right, dumb children. Find The Cheat!
(The Cheat peeks out from behind a box.)
Kids:(half-coherent) He's over there. Right there.
Strong Bad: Um, no, he's behind the box. No, he's not even behind the box, he's barely obscured by the box. (getting upset) Look, The Cheat is behind the freakin' BOX! (screaming) HE'S BEHIND THE BOX!! I'LL KILL YA!! I'LL KILL ALL YOUR DOGS!!!
Flint, Big Bad of Bunnykill 4, does this big time when he kills Ruby, Snowball's potential love interest. Oddly enough, this makes him the only Big Bad of the series to do something truly villainous onscreen.
As of Bunnykill 5, Smoke has joined the dog-kicking party. He has Snowball's friend Dust injected with Psycho Serum, turning him Brainwashed and Crazy and ultimately leading to Snowball's death. For added dick points, he also betrays his partner Professor Sludge, the designer of the serum, leaving him to become Dust's first victim. The second half also has him throwing his own mooks into Dust's way to save his own hide. Not that it helps him in the end.