Owen has often fawned over Justin, and later kissed both Duncan and DJ.
In the first episode of World Tour, Izzy asks Owen and Noah to dress her up as a mummy, she leaves and then Ezekiel comes in, also dressed as a mummy, guess who Owen mistook Ezekiel for and what he wanted to do to him....
Also, Owen trying to "make an alliance" with DJ
Justin in general makes everyone (guys included) lose any and all composure they once had.
In Action, the usually non-affectionate Justin hugs Owen excitedly after he escapes elimination, they continue hugging until they realize that the other contestants are staring at them, at which point they immediately pull back in embarrassment.
Cody and Noah in the episode, "The Big Sleep", ended up sleeping next to each other. Upon waking up the next morning, Noah accidentally kisses Cody on the ear in his sleep. Both awake and begin screaming. Not to mention Cody wakes up first, smiles at Noah, and only begins screaming a few seconds after he realizes Noah is. You can bet fans lapped it right up.
Much later, when Izzy is teasing Noah for this, he emphatically denies this ever happened. This is of course coming from the guy who deadpans consistently even when falling to what would presumably be his death. The Sarcasm Failure that occurred when Trent confirmed it didn't help.
They did it again in the Action special. Only this time, Noah's head was in Cody's lap. Not to mention the position they're sleeping is an exact replica of Duncan and Courtney's famous Sleep Cute pose; the aforementioned couple is even lying nearby in said pose to prove it. And when the two are woken up by Chris' helicopter they don't scream or spring apart as comedy demands, instead they just stay where they are and look alarmed at being woken up so suddenly.
There's also a more subtle scene from back in Island, in the episode right after the one with the kiss, there's a part where Noah talks to Heather and Cody is just staring and smiling at Noah in the background, at first it's not a big deal, since all the Gophers are looking at him, but then after Noah finishes talking and looks down at his book again, everyone else turns back to watch the game, but Cody just keeps staring at Noah and smiling.
With Cody on a separate team and preoccupied with Sierra, Alejandro seems to have taken up his role in World Tour. Alejandro seems to try a little harder than usual to impress Noah. Alejandro's "What say you?" in the Japan episode is blatant flirting, not to mention the fact that Alejandro called him, "my little porpoise" in Newf Kids on The Rock and Noah looked just a bit too pleased when Alejandro offered him a hand. And in the The Am-AH-Zon race, they were sleeping next to each other. Not to mention the Foe Yay as of, "I See London...".
Basically, Noah gets a lot of Ho Yay moments. It helps that he has pretty campy mannerisms.
Don't forget Chris and Chef Hatchet. Those two have become increasingly chummy over the course of the show. Two very prominent examples are during "Camp Castaways" when Chef cradles Chris in his arms for no other reason than he can and in "The Chefshank Redemption" when Chris basically tries to ask Chef out, but gets rebuked. And this isn't even taking into acount Chef's cross-dressing...
Chris's wallpaper is a photo of him getting a massage from Chef.
While they are just friends, it should be noted that Owen seemed to be more distraught over the loss of Noah than the loss of Izzy, his girlfriend. While Izzy may have gotten a whole song dedicated to her, Owen was worried about Noah up until he himself was eliminated!
In Walk Like an Egyptian (Part 1), Alejandro can't turn his charm off, even when he's helping Tyler.
They continue to have odd moments throughout the series, particularly the "pull your headband over your eyes and trust my instincts" bit in Jamaica Me Sweat.
In "African Lying Society", we get Alejandro and Duncan, when escaping quicksand, Duncan hugs Alejandro around his shoulders and lays directly on top of him, not to mention they stay like that for quite a while and Duncan doesn't seem to mind the position.
"Rapa-phooey" provided the show's most prominent Ho Yay example yet: Cody sappily staring up at Alejandro and hugging him around the waist... while sitting in his lap, He then spends the entire episode sucking up to Alejandro, Heather even outright points out that he's fallen for him, and Sierra later calls Alejandro a, "homewrecking husband stealer" for it.
Sierra talks about how everyone on the plane is dating, she then mentions that Chef and Chris aren't, the only reason being, "Chef isn't really Chris' type".
In the episode "Hawaiian Style" Harold gushes about Cody.
"Cody's a deer. And not just for his big blue eyes and tiny tail."
Chris called Harold and Duncan's relationship in Action a 'love fest.'
"African Lying Society" has Ezekiel lying on top of Duncan, both of them fast asleep from the tranquilizer gun.
The relationship between Heather and Lindsay.
And the relationship between Lindsay and Beth.
Beth: I'll miss your fruity lip goop!
Gwen and Courtney had their moments in Total Drama World Tour, prior to the events of I See London and Greece's Pieces, anyway, after which it became Foe Yay.
"Heroes Vs Villains" even had Gwen giving flowers to Courtney (although Courtney was allergic to them)...to apologize for Duncan, of course.
Seriously, Gwen seems more interested in get Courtney's forgiveness that her current relationship with Duncan in All-Stars.
Gwen has now outright (and tearfully) stated that the only reason she came back to the show was to make up with Courtney, and she's devastated because she doesn't think it will happen. Courtney's defenses slipped for a moment after she overheard that, which suggests there might be more subtext for them on the horizon.
In "Suckers Punched", again. Gwen and Courtney have to fight each other in the challenge but refuse to do so due to their reigniting friendship. Chris then uses various clips of Duncan and Gwen kissing to anger Courtney enough so that she attacks Gwen. Gwen fights back and the two girls end up battered and bruised. Gwen eventually admits she only wanted to make things better with Courtney, and Courtney responds that she had gotten over Duncan and never truly missed him after World Tour but had actually missed Gwen. They then collapse to their knees out of exhaustion and embrace each other, saying that they're "friends forever".
In "You Regatta Be Kidding me" they are becoming... in very close friends.
Gwen had some with Bridgette in Season 1; their hug has gotten lots of people talking.
And also Gwen had some with Leshawna in Season 1 as well.
Sadie and Katie are extremely close and are seen hugging quite a lot in Island.
The Ho Yay appears to be toned down considerably for 'Revenge of the Island', but that doesn't mean there isn't any, like, say, the time Scott rips Mike's shirt right off his body...granted, it was to force one of Mike's multiple personalities to take over so he could manipulate him more easily, but that brings up a different topic entirely.
The pilot of Total Drama All Stars has a moment in the jail where a prisoner makes a kissy face at Chef.
Also during the pilot, Jo tells Gwen not to try to kiss her, either.