As tragic as the events of the 3rd trial are to Luke, it's heartwarming to see him genuinely cheer up for a moment at the prospect of helping hear the parrot's testimony. It really does seem to take his mind off things.
Seeing Professor Layton and Maya reunite with Luke and Phoenix, after their apparent deaths.
Luke:[with tears in his eyes]I'd thought I'd never see you again... Maya: YOOOOOOO NICK!
When Layton and Luke discuss Espella's relationship with her father, Layton realizes that this subject matter must weigh heavily on Luke, and tells Luke that if ever he wants to say something to the Professor, he is totally free to do so:
Professor: I'm always ready to listen, Luke.
The Like Brother and Sister relationship between Luke and Maya is very sweet indeed, especially since it's almost immediate. They get on like a cauldron on fire, and converse like they've known each other forever. Just watch them solve a puzzle together.
Maya: Just what I'd expect from the Professor's apprentice.
How easily Maya forgives Luke after he testifies against her. It takes a special kind of person to forgive for something like that.
After Maya's apparent death at the end of "The Golden Court," Phoenix, Luke and Espella are on the run from angry townsfolk. Inquisitor Barnum finds them, apologizes for allowing Maya to die and informs them of what to do in order to escape.
During Phoenix's Heroic B.S.O.D. thanks to Maya being thrown into the fire tavern owner Rouge eventually tells him to snap out of it and challenges him to a puzzle. This, being a Professor Layton game, works.
This needs some elaboration. Phoenix is very tired, physically and emotionally, yet he can't bring himself to rest. Rouge manages to get him to open up first with a puzzle, then Phoenix, one of the biggest Determinators out there, breaks down and starts letting out all about his grief, especially about his inability to save Maya and how he feels inadequate to take care of Luke and Espella. Rouge gives him a Get a Hold of Yourself, Man! speech, reminding him that Luke and Espella are also suffering, are worried about him and it's up to him to take care of them now, then gently empathizes with Phoenix and helps him sort out his feelings. To REALLY drive the punch home, this is playing in the background and tugging at your heartstrings like never before, especially if you've played the first Ace Attorney game, cementing this scene as a simultaneous CMOH and Tear Jerker. And then it turns out Luke was listening in on the entire thing, and he and Espella proceed to become just as supportive of Phoenix as Luke was to Layton and Maya was to Phoenix.
Usually all trials in Labyrinthia end with someone being thrown into the fire. When you win the last one, though, you get the traditional "Not Guilty" message because nobody around is or ever was a witch, and for the first time, you also see the traditional confetti fall... except it's actually flower petals welcoming the new history of the city.
On a similar note, the fact that the Judge allowed Jean Greyerl to live on the grounds that she had not committed any crimes thanks to Phoenix's passionate arguing in her defense, even though she was not his client.
In addition, at the end, His Honor declared that he was actually quite relieved that none of the "witches" he had sentenced had actually been killed, and says that many of the people of Labyrinthia, himself included, would like to stay and live there, even though their memories were returning.
To drive the moment home, it should be noted that, aside the prologue case, the game's rendition of "Won the Lawsuit! ~ The First Victory" doesn't play for the witch trials...until this moment, where Wright and Layton win the last Witch Trial in the history of Labyrinthia. In other words, your first real victory is also your last.
In Edgeworth's cameo, he notes that he's missed hearing Phoenix's "spell" (his trademark "Objection!") while Nick was away in England. Of course, he admits this after insulting Phoenix for the best part of the scene.
Before the long chain of puzzles in Chapter 7, Luke and Layton have a conversation about Maya and Phoenix, talking about how glad they are they met and how much faith they have in the two of them, and discuss what kind of people Maya and Phoenix are. They have nothing but praise, which is a long way from their initial impression of thinking they're just plain weird:
Luke: When I was teamed up with Mr Wright, one thing became clear to me... He's definitely someone that will always do his best to help his friends!
Layton: I agree, Luke. He's a fine young lawyer with a heart of gold. I'm quite confident the trial is safe in his capable hands.
Luke: Me too! And then there's Maya... always strong and courageous! Not to mention full of energy! She's a bit reckless sometimes... but always with good intent!
Layton: We're lucky to have them on our side.
Luke: I'm really glad we met them, aren't you, Professor?
Layton: Yes, Luke, I'd certainly agree with you there. Both Mr Wright and Miss Fey are friends that can truly be relied upon.
When Espella and Darklaw/Eve were falling, Eve twisted them around so she would land first and Espella would land on her, giving her the best chance of survival.
Not to mention the fact that Darklaw/Eve was only falling because she grabbed Espella's arm and REFUSED to let go.
At the end with Espella and Darklaw/Eve. Despite all of the hardships they endured, the two's friendship has rekindled. It's also a happy Tear Jerker.
Espella: (starting to cry) Eve...will you...will you still be my friend?
Eve: .........of course.
Espella: Thank you!
The image following is of a smiling Eve and Espella holding hands, while the Storyteller gives them a fatherly smile.
Espella claims she doesn't remember anything about her friend from the time of the fire, and her father took a lot of effort to repress the memory, but the fact she subconciously named her cat "Eve" and talks about how she's "always been by her side" makes it pretty clear she still remembers their friendship.
During the second special episode, Chelmey is being stern and strict towards Layton, as usual, although when he's asked questions about the current situation he'll wait a few moments before calmly giving the questioning characters the answer they're looking for. When he's pressed on this matter, he says that it's his job to be stern as Layton's rival. Layton calmly informs him that he never saw Chelmey as a rival... but rather, as a friend.
Special Episode 7 reveals that Jean Greyerl was reunited with her parents at the end, as she had no reason to hide anymore.