Applejack being a Team Mom to Fluttershy in thesetwo strips, encouraging her to continue on after she gets knocked out of the sky and begins to doubt her own abilities. She even proposes to split the party and take Fluttershy along a safer but longer route and rendezvous with the rest of the group at the next junction.
As a bonus heartwarming moment, the creator noted that it was at this point in the show that Applejack had become his favourite character.
Rainbow Dash's response to Fluttershy's proposal that she stay behind so as to not slow everyone else down:
No freaking way! No one's getting left behind, end of story.
During the Diamond Dogs arc, it becomes clear that Rarity's chosen profession has created a rift between the Mane Six. When Twilight and the others finally find her, Twilight immediately apologizes for this.