Any time Daby/Davi translates Makoto's inability to admit her true feelings, resulting in the latter's embarrassed blushing.
In Episode 12, when Ira was about to deal the final blow on Mana, the Selfish body he created from Jun's heart freezes and refuses to strike; the boy had so much love and respect for Mana that even when corrupted as a Selfish he could not bear to hurt her.
In episode 13, Alice handing the Royal Yellow rose to Makoto and after being praised for her, Alice was so cute.
Regina slowly starting to see the error of her ways as she interacts more and more with the Precures is this in itself, especially considering her reactions to cute things like Ai-chan seems completely normal for the daughter of an evil overlord who destroyed a kingdom. The crowning moment is in episode 21, when she finally completes her Heel-Face Turn thanks to Mana's kindness. Goes into Tear Jerker territory where she pretty much says she loves Mana and tries to sacrifice herself so Mana can escape from a pit of lava. Mana refuses to let her do this, and it finally completes Regina's Heel-Face Turn, unlocking her power which King Jikochuu sealed away, kick ass and leave the Trump Kingdom.
Youko and Ayumi (Rikka and Mana's mothers) telling baby stories about their daughters in Episode 34. Rikka was a crybaby and Mana was hyper active.
Youko embraces Rikka and tells her how well she has grown up.
Makoto singing her song to Regina in episode 40. This just might be the first time since episodes 21 and 22 that she interacts with Regina on a more positive note.
Also, the other Cures preventing anyone else from interrupting Makoto's song; seeing as they did help write the song, the other Cures want their message to reach out to Reginaas well.
In episode 43 after the Monster of the Week erase the drawing made from Aguri, Cure Ace enter in Heroic BSOD and Manly Tears, but Mari stops the bully from Regina and say that that drawing is from the precious child and the love that her put in a drawing never disappear and after her hug Cure Ace.