Karkat Vantas in Homestuck. He legitimately has a lot to complain about now, but we've seen he's always been like this. Justified since he has bright red blood, a genetic mutation in trolls that is not looked kindly upon. Had the trolls not played the game, he would probably have been denied his dreams and culled as a lowblood mutant.
In Sakana, nothing but his cat (and maybe Jiro being "devastated"...) seems to make Yuudai's day brighter.
Ginger in At Arm's Length. Her boyfriend Geoffrey even introduces her as "the surly one."