Used repeatedly in the blog novelFartago. Punching in the crotch seems to be Farta's preferred method of exacting retribution on Tago for any pain Tago causes Farta. Later justified as the pre-monolith tribe's method of exerting alpha male authority and punishing losing contenders, and also subverted in that the scene when the entire tribe lines up to punch a losing alpha male in the crotch is definitely notplayed for laughs. The cries of anguish of the abused loser depress Farta and Tago so much, they're unable to do anything but drink.
Aaaand...there was the first Nerd vs Critic battle, when they fought with light sabers, but the Critic accidentally had his upside-down...
And during his series, video game confessions, where he's a bartender at a restaurant that sees many video game characters, Samus is apparently the giver of 2 crotch shots, and I mean "with the lazer gun" shot, to both Justin Bailey, and Leisure Suit Larry, because she hates it when people ogle her.
In Kickassia, the Critic finally got to do this to someone else, showing Lee one of the few downsides of being three-dimensional.
In Nostalgia Critic's review of Casper, an animated Casper infuriated by the Critic's panning of his film states that if he can't have a lasting impact on popular culture, he can still have one on his balls. Cue Groin Attack.
In the "You're A Dirty Rotten Bastard" Christmas Special, before meeting his guardian angel, the Critic clamps the poor guy's balls.
Happened a few times in Suburban Knights, especially with Malachite using magic to force Nostalgia Critic to hit himself in the balls, 15 times.
And later on, when stranded with Splinter, Shredder runs off, going, "Man, fuck this shit, you're gonna kick me in the nuts! I'm off this motherfucker! Sheeeeit...fuck you!"
A party themed Happy Tree Friends Smoochie involves the character Nutty getting hit in the groin with a yo-yo. To make things worse, it continues to spin rapidly, with the frition causing smoke, resulting in him bursting into flames, with all the while Nutty screaming in agony. It's the first item on the list, the toy.
A series of videos on YouTube entitled "Kicked in the Nuts" involves a man in an orange afro wig kicking (supposedly) unsuspecting people in the nuts. They invariably react at first with pain and/or anger, until he points at the camera and informs them that "You've been kicked in the nuts!", at which point they're just happy to be on camera.
This episode of Rooster Teeth's Red vs. Blue does this to hilarious effect. It defies description, but not count: 7 separate groin attacks.
In the Super Mario Land series of web videos on Newgrounds, specifically the ending clip of the first episode before the credits, Mario attempts to go down a pipe, only for a fist to emerge from the pipe right underneath him, with Mario expressing a shocked face as the camera pans up to the sky to which a loud holler is bellowed.
In the Dragonball Z Abridged adaptation of "Cooler's Return", the titular villain slammed his leg into Vegeta's crotch with a brief slow motion to make it clear what had happened. A cut later, Vegeta was on the ground asking why Cooler kept hitting him in the dick. Later still, Goku is surprised that of the five hundred Cooler clones Vegeta fought, every one of them punched him in the dick.
The first one was so devastating, Ave Maria was playing.
The prime MO of Ameri-Do-Te and Rachel's specialty, as demonstrated in "Destroy the Groin".
A sparring match between Billy and a student from a rival dojo consists entirely of groin grabs in "Kung Foolishness".
"100 Ways to Attack the Groin" is by far the most watched video Enter the Dojo has yet produced.
Played for Drama
The second installation of Awkward. contains two in quick succession. First Alex against Lester in a staged fight to make Kevin look like a hero (which later leads to some unforgettable mental imagery when Lester remarks, "It's like a cat tried to suck my dick."), then Henry against Kevin. He takes a run at it.
In Worm, Taylor needs to incapacitate Lung as quickly as possible, so she uses her bug control powers to make black widows bite his genitals. Fortunately for Lung, Good Thing You Can Heal is in full effect, at least, once the rotting is finished.