In a (mercifully non-canon) comedic scene at the beginning of insideLG15's "An LG15 Wedding", lonelygirl15's Danielbeast is shot in the crotch by Lucy.
The last attack in the over the top Chris Harry, complete with giant shockwave.
Used repeatedly in the blog novelFartago. Punching in the crotch seems to be Farta's preferred method of exacting retribution on Tago for any pain Tago causes Farta. Later justified as the pre-monolith tribe's method of exerting alpha male authority and punishing losing contenders, and also subverted in that the scene when the entire tribe lines up to punch a losing alpha male in the crotch is definitely notplayed for laughs. The cries of anguish of the abused loser depress Farta and Tago so much, they're unable to do anything but drink.
Another Nostalgia Critic example was the Halloween episode, when the evil Teddy Ruxpin doll bites the Critic in the crotch because of his "mean" review on Teddy Ruxpin dolls.
Aaaand...there was the first Nerd vs Critic battle, when they fought with light sabers, but the Critic accidentally had his upside-down...
And during the newest episode of his newest series, video game confessions, where he's a bartender at a restaurant that sees many video game characters, Samus is apparently the giver of 2 crotch shots, and I mean "with the lazer gun" shot, to both Justin Bailey, and Leisure Suit Larry, because she hates it when people ogle her.
Bennett claws That Guy's nuts when That Guy tries to shoot Bennett for being such a sick bastard.
In Kickassia, the Critic finally got to do this to someone else, showing Lee one of the few downsides of being three-dimensional.
In a Nostalgia Critic review of Casper, an animated Casper infuriated by the Critic's panning of his film states that if he can't have a lasting impact on popular culture, he can still have one on his balls. Cue Groin Attack.
In the "You're A Dirty Rotten Bastard" Christmas Special, before meeting his guardian angel, the Critic literally clamps the poor guy's balls.
And this happened a few times in Suburban Knights, especially with Malachite using magic to force Nostalgia Critic to hit himself in the balls, fifteen times.
In A Very Potter Musical, this is what happens to Snape during the snake attack death scene.
And later on, when stranded with Splinter, Shredder runs off, going, "Man, fuck this shit, you're gonna kick me in the nuts! I'm off this motherfucker! Sheeeeit...fuck you!"
A party themed Happy Tree Friends Smoochie involves the character Nutty getting hit in the groin with a yo-yo. To make things worse, it continues to spin rapidly, with the frition causing smoke, resulting in him bursting into flames, with all the while Nutty screaming in agony. It's the first item on the list, the toy.
A series of videos on YouTube entitled "Kicked in the Nuts" involves a man in an orange afro wig kicking (supposedly) unsuspecting people in the nuts. They invariably react at first with pain and/or anger, until he points at the camera and informs them that "You've been kicked in the nuts!", at which point they're just happy to be on camera.
This episode of Rooster Teeth's Red vs. Blue does this to hilarious effect. It defies description, but not count: Seven separate groin attacks.
In the Super Mario Land series of web videos on Newgrounds, specifically the ending clip of the first episode before the credits, Mario attempts to go down a pipe, only for a fist to emerge from the pipe right underneath Mario, with Mario expressing a shocked face as the camera pans up to the sky to which a loud holler is bellowed.
Green Goblin: "Yeah. The Joker tends to go for the really obvious jokes, doesn't he?"
As part of a montage of Captain Hammer's jerkass humiliations against a helpless Dr. Horrible, he gives the doctor both a knee to the groin and an extremely painful-looking wedgie.
Lady Lumps VS Boy Bumps has hundreds of examples with characters from all kinds of different series. You can even fill out a template and ask the creator for your own character to be in an animation!
Done to a headless (played by Miles) at the end of Spoony's Ultima Underworld 2 review.
Played for Drama
Enjoy. (Not entirely safe for work, contains both comedic and straight examples.)
The second installation of Awkward contains two in quick succession. First Alex against Lester in a staged fight to make Kevin look like a hero (which later leads to some unforgettable mental imagery when Lester remarks, "It's like a cat tried to suck my dick."), then Henry against Kevin. He takes a run at it.
Spc. Crota from Darwin's Soldiers sneaks under a guard and shoots him in the groin.
In The Gamers Alliance, groin attack is Ax's preferred method of dealing with male enemies. Poor Refan gets to experience it first hand.
