At one point, Gwen decides to cheer Peter up by dragging him to a house party. Inevitably, the cops show up and everybody starts to run. After vaulting up a fence, she turns and offers a hand to Peter, with some encouragement about how he can climb it. What makes it is the look he gives her in return is a mix of dry amusement and annoyance.
It's the following entire page of freaked-out, disgusted mental screaming that makes the second one.
Spider-Man, having just taken out Electro and the Enforcers, is confronted by Kingpin. When asked why he is going after Kingpin, Spider-Man responds that this is an important question, and he wrote down his answer because he wants to get it right. Spidey pulls out and unfolds a sheet of paper... and proceeds to read off a list of "you are so fat" jokes that send Kingpin into a rage.
And it isn't the first time he's pulled that. Early in the series, Spidey has a chance to confront J. Jonah Jameson, who's been smearing him in the Daily Bugle, on the street. Spidey pulls in close and JJ thinks he's about to get pummeled before Spidey swings away going "Love the paper! It's HILARIOUS!" JJ sighs a bit of relief and turns to walk back inside only to pull a Face Fault onto the concrete because Spidey quietly webbed his feet to the ground. Apparently Spidey's M.O. against people that irk him is to web their feet to the floor.
When Spider-Man pulls off not one but twoDynamic Entries in the space of a single fight scene:
Spider-Man: Hey!! Did I leave my purse??
After the X-Men take a kid named Geldoff and Spider-Man to the X-Mansion, Professor Xavier basically says he will keep the kid in his custody and present him in front of the UN as an example of illegal, immoral genetic tampering (the kid was a non-mutant that was injected with the X-factor gene in the womb). Spider-Man's response to this is by rushing out of the mansion with Geldoff and beating up everyone in his path as soon as he gets outside the mansion, Xavier appears standing in front of a tree and says "Peter, what good would that do?" Spider-Man responds, "it was just a thought."
That same issue also gave us the X-Men learning Spider-Man's name.
(Spidey wakes up in the X-Men's mansion) Spider-Man: You took off my mask?!! Beast: We wanted to make sure you were still breathing. Spider-Man: Man! I am trying to keep a secret identity here!! No one respects my secret identity!! No one!! One secret! That's all I want. I want to put on the mask and keep it on!! And every time I turn around someone somewhere finds out I'm Peter Parker! (The X-Men snigger) Shadowcat: We, uh, we didn't know your name. (Peter buries his head in his hands)
On the subject of X-Men and his secret identity, Iceman visits his school and we get this:
Iceman: Hey, don't I know you? Peter: No, you don't. Iceman: Yeah I do, you're— Peter:(meaningful glare) We've never met. Iceman: But— Peter: DUDE.
From Annual 1, the scene where Kitty & Peter deal with the Shocker
(Kitty has phased through Shocker's weapons, breaking them) Kitty Pryde: Whatever you do... Don't look behind you. (Spider-Man is on the wall behind the Shocker) Spider-Man: Hi, Herman. Shocker: AIIEEE!!! (Shocker flees, and runs straight into a web)
J. Jonah Jameson blaming Ben Urich for readership of every newspaper in America being down.
The entire exchange with the Fantastic Four discussing Reed making water from his urine (inspired by Waterworld, no less), and then giving it to Johnny to drink.
This little exchange once Johnny Storm decides he's going to be living with Peter.
Johnny: I'm going to do the whole "you" thing. Peter: Wallow in self-pity?
Ultimate Spidey has trouble keeping his costume repaired during a period when he's broken up with MJ and has to go out wearing just his mask and homemade outfit of street clothes. Unfortunately they don't impress the street thugs very much, but at least he still has his mouth:
Thug One: What are you supposed to be? Spider-Man: What? Thug One: The hell is this? Spider-Man: I'm Spider-Man. Read a paper. Thug One: Where's your costume? [referencing how he only has a mask on, the rest of the suit having been destroyed the previous issue] Spider-Man: Your mom's washing it for me. Thug Two:Ooooggh, ddaayyymmmnn!! Thug One: Oh, 'zat howit is? (attacks Spider-Man)
Peter: God! You know why people hate you? It's not because you're mutants!! It's because you're all a bunch of @#$@#$ $@$%@ ##@$!! That's why!! You $^$%^ $%^$ $^$%^ $%#^% #$ $% ^#$%^ $%%^!!! AAAGGHHH! (swings off) Colossus: Why am I an #$@#$@? I was just standing here.
Peter is standing in a lift next to Elektra and can't stop staring at her but glances away every time she looks at him.
After Miles, with no costume or mask, saves two people and a puppy from a fire, two firefighters approch him for a moment before Miles flees. One of the firefighters, who is African-American, smiles and simply tells this to his partner:
Firefighter: Told you Spider-Man was black.
The second Spider-Man's first encounter with Spider-Woman. Poor Miles gets his entire body tied up in web, and tries to hop away to "call the police", only to trip and knock himself out on a satellite dish. It really demonstrates how young and unprepared he is.
Spider-Woman: "Going to the police"... What a dork.
Miles is proving to be quite the smart ass, much like Peter.
Captain Quaid: What's your name? Miles Morales/Spider-Man 2: Uh, Spider-Man? Captain Quaid: Spider-Man's dead. Who are you? Miles: Uh, can I be Thor then?
Also, Miles claiming he's 30 years old when questioned, and Quaid ordering him back home since it's a school night.
Batroc:(after one of his henchmen nearly shoots him while trying to hit Miles, who's on Batroc's back) Moron!! Do you plan on getting paid?? Then stop shooting at me!!
Miles: Where do you get these guys?
Batroc: You would not believe what I turned away.
In his first battle with Venom, Miles has to protect his dad from the symbiote. In order for him to prevent his dad from recognizing his own son's voice, Miles sports a fake British accent to throw him off. It is pretty hilarious as well as pretty clever.
At many points in the game, Spidey has to rescue innocent civilians caught in the crossfire of his fights. At one point, he does so while NOT in costume. Luckily, the construction worker he saves simply comes to the conclusion that Peter is a mutant. Unluckily, he's not excited about getting rescued by one.
Construction Worker: NO! Peter: Mutants are people too! Construction Worker: O-okay. Peter: Say it! Construction Worker: Mutants are people too!