- In "Transform and Roll Out", Optimus Prime asks Sari how humans make "these new, smaller organics" (i.e. babies). His reaction to what he hears is priceless.
- Newly immortal Starscream's death montage in "Mission Accomplished".
- Just about any scene with Blitzwing and Lugnut in it, but especially their first episode.
Hothead Blitzwing: Careful, you copper-clad klutz! You stepped in something!
Random Blitzwing: But with a few dancing lessons, you'll soon be as light on your servos as me, cha, cha, cha!
Lugnut: Silence, Blitzwing! I did not follow this beacon halfway across the galaxy for DANCING LESSONS!
- The entirety of "Return of the Headmaster"'s Sentinel-schadenfreude.
Sentinel [as Optimus fights the Headmaster, who has stolen Sentinel's body]: You're enjoying this, aren't you, Optimus?
Optimus: [smirks while continuing to pound the Headmaster, which Sentinel can feel]
- In the Allspark Almanac databook, a self-serving diary entry from Sentinel Prime makes this even better. "Let me say that again. Optimus. Ripped. My. Arm. Off."
- From earlier in that episode, Optimus's reaction to seeing Sentinel Prime as just a head is gold. Hearing Optimus Prime of all bots laugh is just a great moment.
: Uhhh, Optimus. Tell the organic Fanzone that he can send his police force home. It's a false alarm.
Optimus: How do you know?
: I just know, okay?!
Now I need you to come to my position. ALONE.
And promise me one more thing...
Sentinel: That you won't laugh!
*Optimus looks around the rubble for Sentinel*
Optimus: Sentinel? Where are you?
Sentinel (quietly): Look down...
*Optimus looks down to see Sentinel's head severed from his body, a comedic cue plays
as the camera pans down to the body-less Sentinel. Optimus then lets out a hearty laugh
Sentinel: You promised you wouldn't laugh!
- Also, "You kicked me! On purpose!"
- Sentinel gets a bit snippy, and Optimus can return in kind, even while fighting.
Sentinel Prime: Hurry it up, Optimus Prime! That boat, and my body, are getting away!
Optimus Prime: Would you prefer for me to use you as a shield?! …Actually, that's not a bad idea.
Sentinel Prime: Now, now, Optimus, let's not lose our… I-I mean… youknowwhatImean.
- In general, Sentinel attracts situations like this. His whole visit to Earth consists of him being haughty and then having bad things happen to him. Case in point, his first time out driving, he disrespects human traffic laws and discovers that they're there for a reason when he drives off an unfinished overpass, after having blown through the clear signs warning drivers not to go that way.
- At the end of the Season 2 finale, Megatron in stasis cuffs and Starscream's detached head are stuck somewhere in space, unable to do anything but bicker:
Megatron: Stop staring at me.
Starscream: No. You stop staring at me!
Megatron: Do you think I actually want to spend the rest of eternity gazing at your unsightly visage?
Starscream: Trust me: your visage is no picnic either. And your face is ugly too!
Megatron: Oh, someone just take me offline now...
Starscream: What do you think I've been trying to do for the last four million stellar cycles?!?! AHHAHAHAHAHAHAHAHA! It's funny, really, when you think about it. Tragic, but funny! AHAHAHAHAHA!
Megatron: ...oh, shut up.
- From "The Elite Guard".
[Megatron and Blitzwing appear before him]
Starscream: I stand corrected.
Starscream: Sorry, you must have me confused with some other Harrier jet!
- And then a little later from the same episode (especially considering that Optimus and Co have been spending several episodes trying to convince the Elite Guard that there are Decepticons on Earth):
Sentinel: *Sees a crashed Starscream* What IS that thing?!
Optimus: Huh, that's right, you've never seen one up close. It's called a "Decepticon".
- "Ohhh, you're scared of me! BOOGABOOGA!" "AHH!" The best part is probably Jazz's terrified pose.
- Megatron and Starscream's battle of wills continues in the Season 3 premiere "TransWarped", where Starscream gets so fed up that he starts ranting about how Megatron got them into this mess: "Are you seeing a pattern here? THIS! IS! ALL! YOUR! FAUUUULLLLTTTTT!"
- The 2008 Botcon script reading "Bee in the City" is the funniest thing this troper has ever seen/heard, full of shout-outs and fourth wall breaks aplenty. Watch it here.
- Megatron giving Lugnut orders in "Garbage In, Garbage Out". Seeing how this is Lugnut....
Lugnut: STASIS LOCK ITSELF COULD NOT DETER ME FROM YOUR GRAND, AND GLORIOUS PLAN, OH WISE AND NOBLE MEGATRON!
- And earlier, Sari trying to teach Ratchet some manners:
Ratchet: Will you get in already!?
Sari: Ah, ah, ah, what's the magic word?
- After Lugnut's failed Punch of Kill Everything:
Lugnut: Raargh! Those accursed Autobots will pay for their insolence!
Wreck-Gar: Right! [pulls out a smoking cash register] Cash, check or charbroil?
[Lugnut smacks the cash register aside.]
Wreck-Gar: Right! What kind of tack would you like? Thumb tack? Carpet tack? Income tack?
Lugnut: Argh, never mind…
- During the final confrontation with Wreck-Gar:
You glitch-head! You're gonna destroy the whole city! You wouldn't dare do something that
I am Wreck-Gar! I dare to be stupid!
I will destroy the whole city! Ratchet:
All right, you've forced me to make the ultimate sacrifice; to do the one thing that requires more courage than anything I've ever had to do in my entire Autobot career! I... apologize. Wreck-Gar:
- Heck, pretty much everything said or done by Wreck-Gar in the episode counts!
- After Ratchet saves Bumblebee.
Prowl: I'll stay with Bumblebee.
Ratchet: Try to keep him quiet.
Prowl: You do realize this is Bumblebee.
Ratchet: Oh yeah.
- Optimus Prime, behind you!
- Prowl's attempt at Fake Static at the start of "A Fistful of Energon." Jeff Bennett just nails it in making it so awkwardly out-of-place for Prowl, it's adorkable.
Ratchet: Personally, I've always found Bumblebee's fake static to be a lot more convincing.
- In the first episode, Prowl's reaction to Optimus' 'cog-in-the-Autobot-machine' speech.
- The rivalry between Lugnut and Shockwave during "Endgame". Special mention goes to near the start of Part 1, when after some jibing from Starscream, Lugnut moves in front of Megatron punting Shockwave offscreen in the process.
Can't you shut [Arcee] up? She makes Blitzwing
sound like he's got his head screwed on straight...
Starscream: Y'see, now he even likes Blitzwing better than you. Hmm... *Shockwave meanwhile has walked back onscreen*
Lugnut: *Slaps Shockwave back offscreen* NO! I am the most faithful!
Shockwave: *Walks back onscreen* Have you blown a diode?! I'm trying to work here!
- The best part is when they get into a scuffle, and you can hear Shockwave yell "Liar, lying liar!"
- Finally, while the two do get over it and work together, Lugnut falls over. Shockwave, one of the most stoic and focused of all Decepticons, points and laughs at him.
- From "Megatron Rising Pt. 1":
- In "Megatron Rising Pt. 2," Blitzwing is facing off the Autobots and has the upper hand. Prowl has Bumblebee employ his greatest weapon (his obnoxious personality) and continually talk smack to Blitzwing, who is strafing the Autobots in his jet fighter mode. This upsets Blitzwing, and causes him to switch to his Angry 'Hothead' persona and attack them with his even more heavily armed tank mode. Blitzwing has Personality Powers, but is also an Involuntary Shapeshifter, and his transformations happen about as quickly as his personality changes. It's only when he realizes that he hadn't landed first that he learns Gravity Is a Harsh Mistress.
- Ratchet's mouth hangng open when Sari repairs Prowl in the second episode.
- All of Blitzwing in "Human Error". Even a horrible injury wont faze him for long.
Blitzwing: Oh, look! I have a hole in my rocket!"
- Starscream and his clones.
- Pretty much all of the shorts animated by Studio 4°C are hilarious. Here are some examples.
- "Career Day" has Optimus Prime introduce himself to a group of schoolchildren, but one of them (Daniel Witwicky) keeps pestering him with questions like how he's able to see when in his vehicle form and where his head goes when he transforms. In addition, all the children really want from Optimus is to turn into his firetruck form.
- In "Explosive Fist", Lugnut keeps on punching the ground until it creates a huge empty pit. Blitzwing then comes over in his "Random" persona and tells him that he thinks the pool would look better over there, much to the Decepticon's annoyance.
- "Starscream Heckles Megatron", as its title would suggest, consists of Starscream making fun of Megatron behind his back while he's making a speech. Starscream avoids getting in trouble by acting innocent whenever Megatron turns around, but when Megatron catches him Shaking the Rump, Megatron blasts him in the ass!
- "Operating Table" has Sari help Ratchet perform surgery on Bulkhead. When Bulkhead recovers, he asks about the pile of leftover parts. Ratchet responds by shoving them down Bulkhead's throat, then walks off while whistling the Transformers theme song.