Hana trying to cheer everybody up after Miyuki causes them all to prematurely ditch a stopped train by belting out "Climb Every Mountain" from The Sound of Music.
When Hana attacked that man who insulted her in her flashback.
A woman at the bar's reaction when Hana's mother exclaims "Abandoned!" regarding Kiyoko.
"Yes! I've been abandoned!" *sobbing*
In the abandoned building filled with adorable kitties, when Gin jokes about eating them the cats all understand and surround infuriated cat-lover Miyuki for protection, all snarling and meowing angrily at Gin.
Hana and Miyuki's faces when they momentarily think Gin has died are funny in a Black Comedy sort of way.