Ibuki's storyline from 3rd Strike had her politely challenging Oro to fight as her final exam of her ninja training. Ultracentenarian Oro didn't take the request too well, since he expected that the young girl approaching him was going to ask him out on a date.
Sean's ending in 3rd Strike. It stars with him raising his arms in victory, then fades to him on his back after his opponent beat the everloving shit out of him in the first round of the tournament while Ken tried to wake him up, meaning his ending was All Just a Dream. Oh, and his opponent in the first round was Ken. It was a Curb-Stomp Battle.
Knocking out Twelve with any kind of attack that makes your opponent collapse (usually a LP or LK). He croaks out a "Give...up..." before his body deflates and loses all definition, leaving what looks like a skeleton wrapped in plastic in a heap on the ground.
The infamous Groin Attack in Ken's Second Impact ending... courtesy of his 5-year-old kid. What makes it so hilarious is how the whole scene is shown and paced. Ken is first seen telling someone named "Mel" to attack, but we can't see this "Mel" and it's also the first time that someone named "Mel" is mentioned in the SF canon. Then the scene changes and we see Ken look as if he had been fatally injured, followed by Eliza's horrified screams... and only THEN we see who "Mel" exactly is: the tiny little kid who barely reaches Ken's knees... and has punched his dad in the nuts.The punchline in itself is worth several lulz.