For a doujin game series known for its engaging story, RosenkreuzStilette has some of its own funner moments too.
- Grolla's comically serious moments with Luste Teuber and Schwer-Muta Casasola Merkle in Rosenkreuzstilette Grollschwert.
- Also with Luste in Rosenkreuzstilette Grollschwert, she lets Grolla know that "she's the strongest" if the player loses their final life to her.
- Zorne's reaction to being called an "Idjot" by Lilli.
- Zorne's Birthday doujinshi by Kiora has some funny moments of its own.
- Falling into the many Schmuck Baits of Schwer Stage can be considered as such.
- The Game Over screens in general for humiliating the player with a Shout-Out to one of their favorite video games, visual novels, anime, etc. upon losing their final life.
- After defeating Sichte as Freudia in Rosenkreuzstilette Freudenstachel, Strudel makes herself comfortable in between Sichte's boobs. This, in fact, leaves the normally stoic RKS Strike Force leader herself rather embarrassed.
- The Luste Teuber-centric Rosenkreuzstilette Lustatem April Fools site of 2013. In case anyone misses it, some of the funny things pulled in this April Fools joke includes:
- Luste's body measurements on her profile of B:95cm W:59cm H:90cm when everybody knows she's a Pettanko.
- Liebea's poor brother Kahl wearing Luste's bikini.
- The site featured a two-disc original soundtrack for Rosenkreuzstilette Freudenstachel, with one track on each disc. The tracks are GAME OVER and the Iris Stage map music.
- In the Rosenkreuzstilette Freudenstachel level select screen, one of the characters is presented as "RKS??? UNBEKANNT". Who is this mysterious person? It's Luste with a visor.