A particularly cringeworthy funny moment in Resident Evil 5: during the first battle with Wesker, he may at certain points suddenly shout "Your future HINGES upon this fight!" and then literally kicks you through a door.
The moment you realize that one piece of the fight requires you to turn off the lights so he can't see you is liable to make for an amusing surprise.
When you're exploring the ship, you can actually stop by the bridge, where the captain's chair is. Interacting with it has Chris/Sheva sitting in it and saying something one wouldn't normally say given their situation, before stating that they need to get going.
Chris: "I've always wanted to be a captain."
Sheva: "What a comfy chair."
In the third part of Chapter 2-2, you find a port-a-potty just sitting there for no apparent reason. If you walk up to it, the two options you have are simply labelled "?". Pressing either button causes either you or your partner to knock on the door. As soon as you back away, the door opens up, revealing that a Majini was inside.