The Night After is basically a series of these and heart-warming moments: after a life-threatening adventure against Corona it's a welcome breather episode/epilogue. And then it was revised, adding new gags and fleshing out existing scenes.
The motel being closed due to remodeling, "for [customers'] convenience". Trixie didn't like the irony.
In "Carrot Top of the Line", Apple Bloom claims that Diamond Tiara says that Trixie brings ponies to her basement to kill them and summon monsters. Carrot Top's response? "She doesn't have a big enough basement."
“Why does Greengrass have a big box of mud?” asked Dinky. “I don’t get it.” She tried to figure it out: ”Bwahaha!” Greengrass crowed. “Now that I have all the mud in Equestria, everypony has to do what I want!”
At the Grand Galloping Gala: The Stairs Coup, in which Quick Fix staged a false coup in order to prove to Princess Luna that she should have stairs leading up to her chambers. Luna does not hold back when driving home the point that he could have justsuggested it.
An Awkward Day shows the Stairs Coup from Princess Cadenza's point of view. Once she finds out the cause of the troop presence on her border, she can't stop busting a gut in front of Luna and the other irate diplomats. Even funnier because the only reason troops were posted at the border of her nation of Cavallia (Italy) is because Quick Fix accidentally wrote that when he meant Caballeria (Spain).
In The Jackelope Valley Festival, Vinyl Scratch believes that Corona, "that whole evil-sun-princess-back-from-the-dead thing" is just an old mare's tale. When Lyra and Raindrops point out that not only did they personally fight the Tyrant Sun, but they know for a fact that Vinyl was there when she showed up in Ponyville, Vinyl just shrugs and says that if she believed everything she saw, she'd be crazy.
While mingling at the Gala and looking for a companion for the night, Blueblood finds himself in a position of trying to decide whether to approach Raindrops, Rarity, or Zizanie. Instead of any of them, he ends up with Pinkie Pie.
This hilariously leads to not just his own frustration, but Zizanie's too as Pinkie is so hyperactive that she keeps inadvertently blocking Zizanie's attempts to spike Blueblood's drink with Truth is a Scourge.
There's this line from The School Talent Show. While it doesn't point out who's speaking, the conversation makes it obvious.
Trixie Lulamoon: “....ARE YOU STALKING MY WINDOW!?”
Windowpane: “Miss Representative, your window is putting my kid through college.”
There's also Trixie's reaction to the fact that only her window got damaged.
In Eye of the Hurricane, Trixie wonders out loud if the procedure that turned Raindrops into "Equestria's happiest bully" might be some scheme of one of their enemies, like Duke Greengrass or Corona. Cut to each of them lamenting how boring their day is, and how it's been a while since they last had a plan to mess with the Elements of Harmony.
Chapter 11 of Crisis on Two Equestrias has L!verse Luna and M!verse Celestia and Luna conversing about similarities between their parallel worlds. When L!verse Luna got into the topic on fashion and wigs, picture Celestia wearing a 4 ft (possibly even 5!) powdered wig with jewels and Luna wearing a large frill the size of half Luna's wingspan.
In the Bonus chapter of Helping Hands instead of being turned into a Human, Lyra is instead turned into an Alicorn. Trixie is less than happy for her friend when she discovers the cause of it.
Trixie: Give it back! I wanna be an Alicorn!!
And at the end when it seems all is well. Cue Lyra flying from Trixie who is trying to blast her with a spell to regain her Alicorn Seed.
Lyra: This is not how friends treat other friends Trixie!
Trixie: It is when friends steal stuff from their friends! I wanna be an Alicorn!
In the opening chapter of Up and Automaton, Trixie, bored with the fact that nothing has happened for so long (about a week), has gotten so bored that she has challenged Pinkie Pie to a game of Stealth Hug.
Then there's the Running Gag with the barbershop quartet of rams throwing in random song numbers.
Big Mac gets knocked out fighting the Golem and when he recovers starts spouting and reciting To Be or Not to Be
At the end after it's all settled Cherilee tells Trixie to get her I told you so rant that she promised to do over with. But Trixie only smiles and says she's not going too. Instead she has The Ram Quartet show up around the two and start singing a song about how Trixie was right.
In "A Concert For Ponyville" Pinkie Pie is shocked to learn that Octavia does not eat sweets that she freezes up and can only look on in horror
Pinkie Pie: (Holding a large Knife) "Hi Trixie! I'm glad you're here! I have a special treat just for you!"
Trixie: It finally happened, Pinkie snapped, and now she's going to cut me into little bits and probably bake me into a cake or something. I survived Corona, and Grogar, and the salamanders and all the others, and now I'm going to be killed by a crazy party pony.
In Old Friends its revealed that Cheerilee and Notary (Greengrass's secretary/assistant) were college roommates. They went to Neigh Orleans for the Summer Sun Celebration, got drunk, and then got married. Also, Cheerilee got drunk again the next day and forgot to deliver the annulment paperwork.
The chase scene between Notary and Cheerilee afterwards will make you laugh.
During the Third Chapter, the two enter a Spa as Cheerilee tries to hide fron a furious Notary who is after her and a montage is shown of the two relaxing and enjoying themselves at the spa and having a good time. After that the two exit speaking friendly to each other and then Notary brings up the marriage and the chase is back on.
The non-canon story "Bride of the Rain," has Gilda saying that she has to marry Raindrops. The alternative is that she has to behead Raindrops to avenge her honor... and apparently Gilda's largest dificulty with that is that she's afraid that the Border Control won't let her through with a severed pony head.
According to the story, eventually King Raindrops reconquers all the Griffin Lands, and is scheduled to be named Emperor.
The Non-canon story "Pinkie And Bluie" begins with Blueblood recounting ways he's broken up with ponies- sleeping with her sister, "It's not you it's me", declaring yourself gay, and faking your own death.