Ash dancing to the Pokemon theme song in his pajamas. When his friends catch him, he claims it's a Pokemon trainer workout routine.
Pikachu sleeping under Ash's bed and shocking him when he wakes up.
Everything Giovanni says is funny if you knew Darren Dunstan from Yu-Gi-Oh!, as he uses the exact same voice that he did for Pegasus—before he voiced him, too.
Everything Professor Oak does.
Team Rocket's fourth wall breakage. At a Milwaukee show, after introducing themselves they asked the audience to shout who they were. When they got no audible response twice, James commented that the kids at New York shouted a lot louder.
Meowth tells Jessie and James to be quiet, as "here comes Pikachu and his humans!"
James takes out the pit trap's supports while standing on it. The results are predictable.
Delia and Professor Oak arguing when the supposed Snorlax lecture hall is locked.
Professor Oak: This is very odd...
Delia: Odd? Is that what you call it? Rushing all the way here on a wild goose chase and all you can say is 'This is very odd'?
When Giovanni reveals he set the lecture hall up as a trap, this exchange occurs.
Professor Oak: What have you done with Professor Xalrons? Giovanni: Hmm.... Nothing, nothing at all. Professor Oak: Well then where is he!? Giovanni: Beats me!
Giovanni mocking Oak by calling him Professor Acorn. It's mostly funny since it's Giovanni saying it.
Brock revealing he threw away the map because he couldn't write down a girl's phone number with a map in his hand.
A deaf trainer uses a Jigglypuff, which makes everyone fall asleep... and doodles on their faces.
Everyone screams "NOOOO!" when Jigglypuff appears.
"The Best at Being the Worst" has funny lyrics, and onstage, Jessie and James keep stepping on each other's feet and dropping each other during their tango.
Professor Oak trying to use martial arts training to break out of a cage.
During the Dark Reprise of "You And Me and Pokemon," Professor Oak is seen banging on the cage and climbing on the bars.
Giovanni can't believe that Team Rocket actually caught Pikachu.
James tells Pikachu to "do that thunder thing you always do to us." He does, and shocks every Rocket in the room and frees Delia and Professor Oak.
Jessie, James, and Meowth arrive to capture Ash... on scooters.
When Ash lets Team Rocket capture him, they're completely shocked and wonder why it's so easy; James concludes that maybe it's their lucky day.
Giovanni tells Mewtwo that MechaMew2 is better looking than Mewtwo is.
Giovanni's reaction when MechaMew2 refuses to return Mewtwo's attack.
In the Finale, Giovanni comes out with smudges all over his face and suit and glares at the cast, until the heroes invite him to dance. Albeit reluctantly, he does.