Announcer: Let's see what you folks down in the stands think about our upstar little challenger... Mr. Fuji: I think he's cool. Much Poke-respect. Erika: He's pretty chill. Y'know, for a square. Bill: I think... Lt. Surge:-I THINK HE SUCKS AND HE OWES ME A FUCKING GYM!
A good Mood Whiplash moment. During Ruby's final battle with Gary, after Bruce fails to finish off Blastoise with a Blast Burn and must recharge, Blastoise grabs Bruce, fires off a Hydro Pump point-blank, and misses.
Some moments with Ruby's conversation with Giovanni in the finale such as showing him the Kabuto, and this exchange:
Giovanni: Fourtunantly you still have the Masterball so you can end all of thi- [Ruby breaks the Masterball] Giovanni:[Beat] WHAT THE FUCK Ruby: Sorry Giovanni but rules are rules. ''[Flies away on Bruce]' Giovanni:What rules?!
Giovanni and Maxie in prison. Fucking priceless.
Maxie: So... How do you feel about water?
When Ruby finds Hilbert's body in the closet his response of "Arceus Christ" is just hilarious.
Ruby's reaction to being forced to pick Oshawott. The expressions on Oshawott's face make it even better.
Ghetsis has only just shown up and his speech is golden. "Allow me to reveal a simple truth. Pokemon fucking hate you. Seriously, you capture and pit them against each other their will. Would they fight back if they wanted to be caught?"
And the cop telling them about orders to "liberate" the park. After a complaint about freedom of speech, "Oh yeah? Well I've got freedom of repel." [cue using the pepper spray to get them out]
Fennel's brief flashback of her time at Castelia University with Juniper after Bianca asks how she knows the detailed effects of Dream Mist. The strip makes the use of Dream Mist out like a bad LSD trip.
Fennel: ...I read it in a book. [Ruby knows she is lying]
What made it more amusing was Minccino's expression in the flashback with Fennel and Juniper can be seen as funny given the two were on a Mushroom Samba.
Nuzlocke: Don't do drugs. You'll make Minccino sad.
To add to that, the Plasma Grunts seeing Satan just chilling behind Ghetsis is pretty funny.
Ruby and Cheren decide to team up. Cheren takes things to far and ends up ripping a heart out of a grunt's chest. Grunt's response?
Grunt: What the hell, man...
This moment in the museum:
Burgh: Castelia...? ...That's MY turf!
Ruby: And who are you suppose to be?
Burgh: I'm a friggin' gym leader, who the hell are you?
Ruby: Wow. A gym leader not being in their gym and actually doing their goddamn job. There's a surprise.
And the Plasma Grunt's subsequent reaction of pure horror.
It's even funnier now that there is new merchadise on the site shop showing them in "Kawaii-Mode".
Ghetsis and Bronius discussing people from Johto and their calling habits.
"There is nothing wrong with people from Hoenn" in the same panel with Ruby about to break into a window on a vehicle.
Robotv7's Nuzlocke Challenge (FireRed and Sapphire):
From Robot7's comic there's his attempt to make his Hitmonlee more unique.
Let's see what TMs we have... *finds Attract* oh god yes...
Also from Robot:
Robot: Man, i should switch Pchooooo out... (Beat) Naw, you can do it Pchooooo! Pchooooo:[sweat] Robot: We need a critical hit. Not a critical hit. Robot:[gasp] Pchooooo:[tears] Machop used..Focus Energy!
Robot altering Dewford's trendy phrase.
Kid: Are you sure...? Robot: Positive. Kid: OK! Hey everyone! The new trendy thing to say is ADULT TOYS!(Robot runs off laughing)
Freddy's Nuzlocke Adventure:
Rosie's reaction to her first real battle being against an Onix. That face is priceless.
Cook decides that his team needs a water Pokemon, so when he sees a lone Wooper he sends Audrey the Hoothoot to hypnotize him until K.O., then he and Belmont (the aftermentioned Ekans) carry him away and convince him to join the team.
In Fatal Platinum, how does our protagonist keep Lucian out of his mind? By conjuring up mental images of the most grotesque, hilariously vile things he can think of. The only thing specifically noted is "Pichu/Miltank pairings," but... well. And then when Lucian tries to read his Medicham (who has been a serious character throughout the run):
Lucian: Brrrr...let's see what you have in YOUR mind instead. A fellow psychic would never-
Diell: Hippowdon lapdance.
Lucian: OH COME ON!
Manic tearing down the Voltorb Flip mini-game in Spirit Electrum to get thousands and thousands of coins, and the team's proceeding reactions.
*Manic advances to Lv. 7 of Voltorb Flip*
Link:WHAT THE FUCK.
Manic: What? It's easy.
Will: The old guy is staring at you like you've got a tree growing out of your head!
Gail: It's only a matter of time until you make a fatal mistake!
Link: Ha! Gail's right. I mean, sure, you got this far, very nice. But this is the maximum level. There's no way you can-
*Manic clears Lv. 7 as well*
Link: What are you?!
From Dennis's Revenge: The Johto Takeover:
Falkner: Dennis, why do you seek to fix what Arceus (who is masquerading as Silver) has done? Dennis (actually Genosect in human form): Look at this place! Legendaries all over, level 3 fully evolved Pokemon, even Pokemon that don't belong in their regions are here! And you still have your two derps! Falkner: You did NOT just call my precious birds DERPS! I'll take you down! Go, Pidgey!