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Hulk Vs. Thor
Hulk Vs. Wolverine
The Stinger at the end of the Wolverine segment:
Deadpool crawls out of wreckage*
Deadpool: ...I can't believe it! I'm alive! I'm aliiiiiiiiiiii-
Gets stomped on by Hulk's super leap*
Way to make me nearly drown on dry land, fellas.
All of Deadpool's dialogue, which was 90% improvised by Nolan North.
"Noooooooo! Thaaaaaat waaaaaaas my favoriiite gunnnnnn!"
"Logan, buddy! It's me, Deadpool! I shot yooooou. :D"
"Hey, do you still have that indestructible skull?"
* metallic ping* "OWW! Dammit..!"
"Who wants snacks? YOU DO!"
"Logan, I think we lost him!"
"And I shot 'im in the ass, with one of these! What? I did."
Deadpool: Logan. We missed you. The Weapon X isn't the same without you. Nobody calls me "bub" anymore! And Omega Red's a bed wetter! * just then Omega Red enters with the Doc*
Omega Red: One day I will tear out your flippant tongue, Wilson.
Deadpool: [whispers] He's very ashamed.
Deadpool loves pointy things.
Professor: We put considerable time and money into you.
Deadpool: And pointy things!
"You know, I'd would think that the last thing he said was, 'AAAAAAAAAAH, Sabertooth!'"
"Strike a pose!"
"BANG! Just kidding."
Deadpool struggling to get his arm back together.
" HULK! HATE! CLAW PEOPLE!"
Hulk and Lady Deathstrike's yelling contest deserves a mention.
On a meta note, Fred Tatasciore and Steve Blum recorded together and had to be pulled apart slightly, because the pair were acting it up so much that the staff were worried they'd both clock each other.