- Issue #0 has quite a few funny moments as Harley goes through several artists and their art styles.
Batman: Bruce Timm would never let this happen!
- The start of the issue has her in a warehouse reading comics from DC's New 52 and wanting to have her own comic. Wish granted.
- Harley is surprised that they managed to get Jim Lee's expensive self to do a page. She's bummed when she realizes that the page is a remake of a scene from Batman: Hush. Except this time, she beats Batman.
Harley: Is she serious?
- The writers accidentally call Batman by his last name, and he calls them out for revealing his identity. Also, the sandbags in the scene are said to be more effective this time around because they're filled with Jim Lee's quarterly royalty payments.
- Harley hates how sugary sweet and childish Art Baltazar's art style is. She hates even more how nobody bleeds.
- Bruce Timm's page has her doing a theater rendition of her "Rev up your Harley" scene from Batman: The Animated Series. She forgets her line and ends up imagining herself naked in a choir singing her line.
- When Harley mentions the Suicide Squad, the writers misinterpret it, so the artist has her in situations that would be considered suicidal, thinking that's what they do. (These situations are: Harley wearing a chicken bikini in an alligator swamp, tickling the roof of a shark's mouth with a feather, and riding on a missile in outer space.)
- In one scene, she and Catwoman attend the wedding of the book's writers, Jimmy Palmiotti and Amanda Conner, and try to stop them. Amanda tells Jimmy not to hurt Harley because she's the one paying their bills.
Catwoman: You see the numbers on All-Star Western and Batwing? noteHarley: Yeah, let's go easy on him. Maim, not kill.
Amanda Conner: I'm Amanda Conner, bitches!
- When they beat up Jimmy, Amanda gets mad and punches them out for hurting her husband, leading to this gem:
- Conner wants Darwyn Cooke to be the book's artist because the illustrations made her kick ass. Harley objects, since it's supposed to be her book, not Amanda's.
- The variant cover for issue #0 has Harley being watched by several villains. Most of them watch her sternly, but Catwoman looks surprised and the Cheetah looks a little too interested in her. Bizarro isn't even looking at her, he's facing the other way with a dopey look on his face.
- Issue #1
- As Harley is out looking for a new place to live, she sees a dachshund being dragged behind its unconcerned owner on a bike. That part is not funny. The Puppy-Dog Eyes being escalated and exaggerated over the course of several frames, however, is.
- Issue #2
- In the opening, Harley appears to be back with the Joker, but she's revealed to be straddling a wax statue of him. The owner of the wax museum tells her to stop dry-humping the displays.
- To save a bunch of animals from dying in an animal shelter, Harley fills out a form to see if she's eligible enough to adopt them. The secretary takes one look at the form and tears it in half.
- When first informed of the animals' plight, Harley asks the group of protesters gathered outside if they had considered adopting some of the animals. Turns out, every last person present already has adopted dozens of them.
- Poison Ivy scares Harley by playing ventriloquist with a dead guy. Harley's reaction is priceless. Even her dog freaks out.
- Ivy and Harley break the animals loose from the shelter, evidently expecting them to obediently follow them home. Instead, Reality Ensues and the girls have to run across the city rounding the animals up after they immediately scatter.
- Issue #3:
- The love berry that Harley eats makes her smell irresistible to everyone she meets. The kicker is a female cop who falls in love with her because, to her, Harley smells like really great donuts. The cop's also confused because she didn't think she was into women.
- Issue #5
- Harley goes to a burlesque theater and thinks the staged fight is real. She tries to stop it, making the audience throw rotten tomatoes at her (which the concession vendor was selling for just such an occasion).
- One of Sy's enemies is in a coma, but is surprisingly hard to kill. After two failed attempts, Harley steps in and blows into the man's breathing tube. The results are Bloody Hilarious.
- The issue ends with Harley and Sy getting caught in an explosion. The ending narration tries to pull a cliffhanger before admitting that they'll be alright.
- Issue #6:
Enemy: Any last words?Harley: Pneumonoultramicroscopicsilicovolcanoconiosis!Enemy: What is that?Harley: Longest word in the dictionary. As far as last words go, it's a doozy.
- Harley has a squee attack when Sy and Zena (an enemy old woman/old flame) have a kiss. This is made even funnier because Harley is inexplicably upside-down.
- Sy spends time chewing out an old enemy of his for giving him a car with an engine leak, that forced him to leave the car on the road, leaving it vulnerable to robbery. He complains about how he might still have the car if the man had told him about the leak, and when he mentions that he might have given the car to Harley, she promptly sends him out the window because she could have had it. The scene displays some hilarious Skewed Priorities, especially when Sy complains about Harley's Kill Steal.
- Harley is cornered:
- Issue #7
- Harley acting like an annoying younger sister when Ivy wants her to go to sleep in order to catch whoever's putting a hit on her. Harley begs for a bedtime story, a glass of milk, and even asks if the person's there yet while awake, when she needs to be asleep for the plan to work.
- The reveal of who put the bounty on Harley's head: Harley herself, while she was sleepwalking.
- Harley and Ivy's bizarrely casual bet about which side of the fence an assasin's body will fall over on. The fence rips the body in half instead, causing it to fall on both sides, squicking out the girls in the process.
- The beach guy Harley brings home. He wakes up and realizes his situation a few times, but Ivy puts him back to sleep because they're busy. Big Tony is also confused as to why Harley brought him there.
- Issue #8
- Everything to do with the Scatapult, which they aim at Fresh Kills on Staten Island (instead hitting Brooklyn Bridge), the Atlantic Ocean (hitting the Ferris Wheel at Coney Island), the J Train (successfully hitting the moving train), and finally hitting DC Comics Headquarters as Dan Didio proposes (yet another) DC comics reboot/relaunch.
- Issue #12: Harley mishears Queen Eidijamon's name as "Queen I Did 'Jer Mom".
- Issue #13: After PowerGirl's memory comes back, Harley has to desperately convince her not to beat her up. She succeeds, but then takes it a little too far by suggesting maybe they could "go downstairs and slip into..." Which is as far as she gets, because Kara leaves her stranded on top of the Eiffel Tower.
- Issue #3.15's alternate cover; especially Power Girl's (lack of) response.
- In the SDCC Special
- Annual Special
- The signs at the nursing home, saying "Bless my Mess" and "Please try not to wet yourself".
- The small moment in Issue #19 when Capt. Strong comes out even more hulked out than before (in what is essentially a thong made out of alien seaweed) and starts spouting a list of metaphors for crapping. In response, Harley... spouts a longer list of metaphors for puking.
- As part of Harley's plan to bust Mason out of Arkham Asylum, Poison Ivy secures a getaway boat. Ivy mentions that they need to move fast, because the boat's owner doesn't know she has it yet. The getaway boat? The Batboat.