Yes, even a game about eldritch horror has its rare amusing moments.
When the ghost of Chandra appears to Karim at the end of his chapter, he lists off all the ways she wronged him, and ends the list by saying that she "really doesn't look so good anymore", complete with leaning back and a look of mild disgust. When he agrees to stay with her, his final words as a mortal are more annoyed than anything else.
Attempt to set the wrong rune as the ghost of Edward when he appears to bind Alex's summoned Ancient enough times and Edward will murmur to himself over how rusty he's gotten with them. "It's the squiggly circle thing!"
Anthony apparently has no feeling in his hands once he gets a hold of the Two Handed Sword - he uses his bare hand to test its sharpness without so much as flinching.