The licorice whip aliens they dub "Twizzler Demons."
This memetic exchange from the beginning of level 6:
Captain Novolin: Hello Ranger. I have diabetes. Is there anything special I should know before climbing the mountain? Diabetus: Dude, you gotta think of a better ice breaker than that. [slowbeef laughs] Ranger Betty: Thanks for telling me. slowbeef:"Thanks for telling me?!" [...] I have herpes, is there anything I should know before climbing the mountain?" "Thanks for telling me!" Yeah!
Then, immediately after:
Text box: Check you feet for... slowbeef:WHAT?!... Check you feet![cracks up] Diabetus: No guys, do it! Guys, do it right now! Like, right now! Scarboy: Wait— wait, it said "Check you feet..." slowbeef:[in a country voice]"CHECK YOU FEET BEFORE CLIMBIN' THIS MOUNTAIN." [Captain Novolin ducks to dodge a Twizzler Demon; his pose looks like he's staring at his foot] slowbeef: And you did! [howls with laughter]
Shortly afterwards, they take notice of his damage animation, where Captain Novolin kneels and clutches his stomach.
slowbeef: I like how he gets dizzy whenever he gets attacked, and holds his stomach. Diabetus: Well, he just got attacked with sugar, I guess. Scarboy: Whenever he gets hit, I think he looks like a giant pansy. He's like, "Oh no, you just hit me in the stomach!" Diabetus: "Uuurrgh, That really hurts, ugh!" slowbeef: "I'm nauseous from fighting, guys!" Scarboy: "Stop hurting me!"
Level 7 manages to top this somehow:
Captain Novolin: Whew, what a hike. Hi, Ranger Ralph. I am looking for the mayor. But just in case I get into some trouble, I want to let you know that I have diabetes. [everyone laughs, slowbeef especially hard] Diabetus: Wait, what's in the— [the screen fades to show a certificate] Certificate: I HAVE DIABETES - If I am found ill or fainting, please give me some sugar. If this does not revive me, please call a doctor or an ambulance immediately. [slowbeef begins laughing with renewed vigor] Diabetus: This is like... this is like a college diploma! What is this? [slowbeef continues laughing] Diabetus: Does he like, strap that to his chest so when he faints, people can see it? Ranger Ralph: Thanks. slowbeef:"THANKS?!" Diabetus: I imagine he's saying that really awkwardly, like, "Thaaanks..." slowbeef:Oh my god, you've got a certificate!!
slowbeef misunderstands the final level's question:
"This video game is quizzing you on why it's good to eat?!"
The final boss of the game: a strange, middle-aged-looking man with a headset who sits in a chair and fires pies at the player, and dies in three hits from electricity activated by a nearby switch. slowbeef's hysterical reaction, again, makes the video.
In the next video, Diabetus fights through That One Level with slowbeef alternating between mocking him and insisting they're gonna edit stuff out, right down to Diabetus doing introductions every time he dies and restarts. Bonus points for a few well-placed Description Cuts to his off-screen rematch with Yakuza.