The voice he does for Olimar (with electronic help) and in particular the segments he does at the start of each episode with Olimar in orbit talking about his plans, only to be interrupted by the Pikmin in their Onions having wacky hijinx.
Tyler: I don't want to name them after, like, Youtube usernames, because I don't want to be naming the Pikmin, like, "ilovezelda69" or like "linkfuckingmeuptheass200" or something...I know some of you guys have weird usernames like that. Don't lie to me.
He keeps trying and failing to come up with a sexual Parody Name for the Forest Navel region. By the time he leaves in episode 7 he mixes several failed attempts together to make "the fucking ninja's furry nipple".
Tyler's Pax Prime 2012 vlog has him meeting several other LP'ers for the first time, and all of them greet him with "My God, why are you so tall?!"