Faceless Goons: Inverted. All of the Red Skull's mooks are well-dressed highly attractive people. The military force that comes to rescue President Kimball and help Captain America come equipped with face-concealing masks and all-black uniforms.
Faux Affably Evil: The Red Skull. As he has Captain America tied to a rocket, he asks him for help with his English.
Hero Stole My Bike: Captain America feigns sickness to lure the unsuspecting driver out from the car before he steals it, twice. Subverted when he appears to steal a bike from a civilian during his and Sharon's escape from the Red Skull's thugs, but he throws down money towards the owner for compensation.
Mistaken for Spies: Captain America thinks that Sam Kowlawetz is a spy for the Japanese, because he has a tape recorder that was made in Japan.
Or possibly for the Germans, because he's driving a car that was made in West Germany. (This is the first car he steals, under Hero Stole My Bike.)
Mythology Gag: When young Thomas Kimball tells his friend Sam about Captain America, they discard the idea of the hero having been the Human Torch or the Sub-Mariner, heroes who fought in World War Two along with Cap in the comicbooks.
Negated Moment of Awesome: Captain America spent so much time frozen in Alaska, he didn't get to accomplish nearly as many awesome things as most of his other incarnations have.
Not So Different: Captain America and The Red Skull both got their powers as a result of the same research. Because of this, the Red Skull insists on referring to Captain America as his "American Brother".
The Only One: But not by design. The scientist working on the project did not have any written notes, for fear that they would be used for evil again. Shortly after Rogers is successfully given the treatment, a Nazi assassin kills the scientist and her knowledge is lost. Otherwise, the intent was for him to lead an entire company of super soldiers.
Race Lift: The Red Skull's nationality is changed from German to Italian for this film.
Raised by Orcs: The Red Skull is raised by Nazis after being abducted from his family.
Refuge in Audacity: When the recently-escaped President Kimball runs into General Fleming, in the Red Skull's hideout, in a business suit, the general stands at attention and claims that he is leading the rescue force to extract the President. The President doesn't buy it.
Sherlock Scan: Notably, it gives Captain America all the details needed for him to reach exactly the wrong conclusion. note He doesn't know that World War II ended over four decades ago, and that owning a Japanese tape recorder would not at all be unusual for an American.
Strapped to a Bomb: The film has the hero tied to a rocket at the end of the first act, setting him up to be frozen and appear in modern day.
Red Skull: Assassination isn't worth the trouble. It took me two years to find Sirhan. Three to find Oswald. The King job alone cost me over twenty million dollars. What do we get for our pains? Saints. Martyrs to the cause.