Will Not Be A Victim: Only Hydro-Man has ever really shaken her, and even then she'd still stand up to him.
Felicia Hardy/Black Cat
Peter's first Love Interest on the show, Felicia's life becomes a lot more interesting after The Kingpin uses her as a test subject in an experiment involving Super SoldierSuper Serum. She becomes the Black Cat, a vigilante who is a frequent ally to Spider-Man.
Super Powered Alter Ego: Not as polarised as most, though her personality alters slightly upon changing. While the Cat proudly boasts about her initial robbery, Felicia is immediately disgusted upon changing back.
Mad Doctor: Has many of these traits as the Lizard.
Mentor Archetype: Is one of Peter's biggest inspirations and, as Mary Jane mentions one, like a father to him. Is occasionally The Obi-Wan, and the fact that he and Peter have such a close relationship is why moreso than anything else he strives to help him when he becomes The Lizard. Connors also plays this role with Debra Whitman.
Misanthrope Supreme: Hates humanity and seeks to turn them all into reptiles like himself in all appearances after his first.
A classmate of Peter's, Harry eventually becomes his roomate and closest friend. Unfortunately, his breakup with Mary-Jane and subsequent jealousy over her relationship with Peter, coupled with the revelation that his father, Norman, was the original Green Goblin unbalance his mind, leading him to adopt the identity for himself.
A blind attorney who represents Peter during his trial for treason, Matt Murdock's father was murdered on orders from The Kingpin. He maintains a doublelife as the vigilante, Daredevil, and helps Spider-Man discover the truth about The Kingpin's identity.
Disability Superpower: The same accident that blinded him also heightened all his other senses to superhuman levels.
Living Lie Detector: Which lets him see through most of the Chameleon's bluffs. But it somehow didn't help him realize sooner that Spidey was framed and also that the Federal Agent asking for the evidence clearing Peter was The Mole.
Plucky Girl: She takes no crap from her egomaniac boyfriend, realises that Spider-Carnage is a fake and saves our Spider-Man, and then helps drive off Spider-Carnage by opening up on him with a sonic rifle. If it weren't for her kidnapping and five minute appearance she might be a full-on Action Girl.
Spot the Impostor: Instantly recognises Spider-Carnage as a fake. She also seems to have figured out that our Spider-Man isn't hers, although she still recognises him as a hero.
Spider-Man: How'd you know that the Peter Parker downstairs wasn't me?
Gwen Stacy: He's as nutty as a fruitcake. A girlfriend notices little things like that.
Mysterious woman who takes it upon herself to be Spidey's mentor, preparing him for "the ultimate battle"
The main villain of the series, the Kingpin operates a criminal empire with its headquarters in New York City. He is The Man Behind the Man to many of the villains in the series, including all the incarnations of the Insidious Six and the Spider-Slayers.
Amoral Attorney: Not our Kingpin (who pretends to be a businessman and philanthropist) but the version of Fisk in the Iron Spider-Man's world definitely qualifies.
The Man Behind the Man: He's the Big Bad to the Insidious Six, the Crime Cartel, and almost every other major supervillain on the show, but he keeps to the shadows and Spider-Man does not meet him face-to-face until the third season.
Take Over the World: Would love to do so, although he's fairly practical about it, focussing more on spreading his influence than crazed schemes—although he certainly has a few of those up his sleeves.
"Well Done, Son!" Guy: In the backstory. He is pleased that his relationship with his son is nothing like the one he had with his father.
Spencer Smythe's wheelchair-bound son, Alistair becomes The Kingpin's right-hand man for much of the show, serving as his chief inventor and confidante. He is later transformed into a living Spider-Slayer by The Kingpin and Landon.
Antivillain: At first he's being played by The Kingpin; later he's trying to save his father.
Ascended Extra: In the comics Alistair is a fairly minor villain. In TAS he's an Evil Genius-for-hire whose inventions play a vital role in the schemes of half the crimelords in New York.
Once a respected scientist, Dr. Otto Octavius was Peter Parker's mentor as science camp when he was ten. Following an accident that bonded his tentacles to his body, Octavius has become a criminal mastermind, though he still has a soft spot for his former students. He is a core member of the Insidious Six and acts as their field leader.
Adaptational Attractiveness: Octavius in the comics is a fat, ugly man with a bad haircut. This version retains the bad haircut and square face but has the physique of a body builder.
A former private investigator who went mad after Jameson had him transformed into a weapon to use against Spider-Man. He alternates between working as a mercenary for The Kingpin and other crime lords (most notably Silvermane) and wanting to return to normal. A member of both incarnations of the Insidious Six.
Body Horror: In Gargan's first episode, he starts to spontaneously mutate further. His upper torso swells (in a manner that is implied to be very painful) with muscle until he looks ridiculously top-heavy, he grows to be about twelve feet tall, he develops green skin and yellow eyes, and his fingers start forming into claws. In subsequent appearances, he looks like his pre-mutation form (a tall, muscular but normal man in his trademark costume).
The Brute: Shares this role with The Rhino as a member of the Insidious Six.
Chronic Villainy: No matter how hard he tries, or what he promises Sarah, Mac just can't seem to keep out of the underworld, ineveitably falling back into crime whenever he is short of cash.
Dumb Muscle: He's not out and out stupid like The Rhino, but Mac is still hot-tempered, impulsive, and dangerously short-sighted.
Even Bad Men Love Their Mamas: Yells at Spider-Man about not dragging his mother into things when the latter cracks a "your mom" joke. This is especially notable, because Spidey had tried to press his Berserk Button (as above) in the process, but it's only the comment about his mother that riled him up. In the past, just insulting him would have been enough on its own.
Even Evil Has Loved Ones: Later episodes reveal he has a fiancee named Sarah, who he loves and who helps hide him from the law, and he wants to go back to being Mac Gargan so he can get married to her.
Evil Counterpart: Created through the same process that made Spider-Man what he is, and is also based upon an arachnid in terms of both costume, name and abilities.
Hair-Trigger Temper: Scorpion goes berserk very easily, which usually brings about his downfall.
Hellish Pupils: His pupils become vertical slits as his mutation progresses.
Hero Killer: Subverted. He's dangerous certainly, but despite the buildup, isn't significantly more threatening than other members of the Six (and less so than some).
I Just Want to Be Badass/I Just Want to Be Normal: Starts out as the former, but progresses to the latter. It's implied the change-over has something to do with the painful body-warping freakout he undergoes in his first episode, but he reverts back to his "normal" Scorpion form afterwards (which, honestly, is better looking than his human form) and never seems to be at risk of mutation afterwards.
Save the Villain: One of the few occasions Spidey did it knowing he was saving a villain.
Scary Scorpions: Chosen for the symbolism; Scorpion was created to defeat Spiderman, and scorpions are natural predators of spiders.
Super Reflexes: As Spiderman's evil-counterpart, he can move and react with great speed; in his first appearance, he actually claims (and is shown to be) faster than Spidey — however, he lacks Spiderman's precognitive Spider-Sense.
"This time it's maximum voltage at minimum range."
A mercenary in The Kingpin's employ, Shocker fires vibrational waves from his gauntlets. He is a member of the Insidious Six and tangles with Spider-Man as part of the group and on his own.
Achilles' Heel: Downplayed. Water can cause his gauntlets to short out, but it's only a serious problem for him if his insulated suit is also damaged. This convenient allignment of factors happens once in the entire show.
Adaptational Badass: Shocker in the comics is treated as a joke, regardless of his (not unimpressive) track record. This version managed to give the black-suited Spider-Man a run for his money and is given the appropriate amount of respect.
Never My Fault: His careless use of explosives got him one year in prison and he blames Spider-Man for this despite Spidey's role being of saving people from the explosion, thus saving him from a larger time in prison.
Unholy Matrimony: Eventually develops a crush on a badly disfigured (and totally unhinged) actress.
Adrian Toomes/The Vulture
A failed businessman with a grudge against Norman Osborn, the Vulture is an old man who eventually develops a way to feed off of his victims' youth in order to temporarily become young again. By stealing the rejuvenated Silvermane's youth he is able to make the process permanent; he then joins the Insidious Six as Mysterio's replacement.
Cut Lex Luthor a Check: The idea of showing the results of his antigravity research to the executives to persuade them not to let Norman Osborn take over has never been discussed in-universe. His comic book counterpart would feel disappointed.
Shapeshifter Mode Lock: For a while his power was on the fritz, causing him to shift between old and young at the drop of a hat. He eventually managed to freeze himself at young by stealing Silvermane's newly accquired youth.
Never My Fault: Eddie Brock blames Spider-Man for his failed career as a journalist. Sure, Brock may blame Spider for being unable to expose Kurt Konnors as The Lizard but it's not Spidey's fault that he mistakenly exposed Flash Thompson as Spider-Man; lost a recently-gotten job as a result of a spider slayer attacking him; or that he destroyed his reputation by framing Spidey with the theft of a rare mineral.
A criminal lunatic, Kasady had the cell next to Eddie Brock. Baron Mordo offered him the chance to gain the same kind of power that Brock had, granting him one of Venom's offspring as a gift and transforming him into Carnage.
Empowered Badass Normal: Cletus Kasady held off the NYPD and Spider-Man using his own cunning and liberal doses of high-explosives. Needless to say, he becomes an even more serious problem after he turns into Carnage and completely averts From Nobody to Nightmare. When it's discovered that it's Kasady behind Carnage's mask, everyone becomes more frightened.
Slasher Smile: Frequently shows one as Kasady; gains a permanent one as Carnage.
The Sociopath: The clearest-cut example on the show. Kasady likes to hurt people, has a Hair-Trigger Temper, expresses no remorse for his actions, and has no regard whatsoever for human life, his own included.
A killer created and retained by Norman Osborn for the purposes of assassinating The Kingpin, the Hobgoblin eventually turned freelance criminal. Who was behind his mask remained a mystery for much of the show; it was eventually revealed to be Jason Phillip, Felicia Hardy's fiance.
Chronic Backstabbing Disorder: He backstabs Norman Osborn, The Kingpin, Landon, and anyone else who crosses his path over the course of the show. Special note should go to his introductory two-parter. He doesn't just double-cross, he doesn't just triple-cross, he goes for the very rare quadruple-cross.
Composite Character: Acts alot like the original Hobgoblin (Roderick Kingsley) but is Jason Phillip Macendale beneath the mask.
Was It All a Lie?: Felicia asks him this ,and if he ever really loved her, after he's unmasked and captured. With nothing to lose, he answers honestly, that he isn't sure, that there were things about her he loved: her wealth, beauty, and refinement. Felicia quickly decides that that definitely wasn't love.
Norman Osborn/The Green Goblin
The father of Peter's friend Harry, Norman is a crooked businessman who starts the show almost completely under the thumb of The Kingpin. A lab accident eventually results in him developing a split personality; donning an altered version of the Hobgoblin's gear he becomes the Green Goblin and seeks revenge on everyone who has ever made his life difficult.
Adaptational Villainy: Completely averted. He's by far the most sympathetic version of Norman in any media, more a victim of circumstance than the brutal monster of the comics.
Antivillain: Norman's a fairly nice, if weak-willed guy who's in way over his head with The Kingpin. Even the Green Goblin persona can be seen as something of a Knight Templar rather than the Axe Crazy loon he's normally portrayed as.
A member of the Crime Cartel and a rival of Wilson Fisk's, Silvermane is governed by two obsessions—to seize control of the cartel from The Kingpin, and to regain his lost youth.
Be Careful What You Wish For: Wished to be young again. Was turned into a baby. He then wished to regain his adult body—and was aged to an even older state than beforehand by The Vulture. Feel free to laugh.
Butt Monkey: He gets screwed over by Fisk, The Scorpion, and The Vulture (not to mention Spider-Man), and all of his schemes backfire horribly. You'd feel sorry for him if he wasn't such a dick.
A childhood friend of Robbie Robertson's, Lonnie Lincoln was forever changed following a mishap at a chemical plant. He now operates as an underworld hitman, taking jobs for the likes of Silvermane and Richard Fisk.
Revenge: When they were kids, Robbie left Lonnie in the lurch following a corner store robbery; Lonnie went to juvie. As an adult he tried to frame Robbie for a crime he didn't commit in order to get back at him.
Revenge by Proxy: Following his transformation into Tombstone, Lonnie tries to frame Robbie's son in order to get back at him.
Super Strength: Easily in the running for strongest character in the show.
An evil sorcerer with ties to the netherworld, Baron Mordo works tirelessly to free his master, the dread Dormammu from his prison. To that end he has brainwashed innocent people, stolen souls, and even conspired with Venom and Carnage.
The son of the Red Skull, Schmidt spent most of his life trying to free his father from the vortex. Manipulating The Kingpin and Silver Sable into doing his bidding, Schmidt ultimately freed his father who "rewarded" him by transforming him into his ultimate weapon, Electro.
Adaptational Badass: Big time. He brings down all of SHEILD with a gesture, and is too powerful for Spider-Man to confront directly.
"Do not despair Chameleon. The Red Skull anticipates everything."
A Nazi spymaster, and archfoe of Captain America and the Six American Warriors, The Red Skull was trapped in the vortex alongside Cap. He is eventually released by his son, Rheinholt, whom he transforms into Electro; following Electro's betrayal, he and Cap are once again trapped in the vortex.
"This is what I have always wanted—absolute power and control!"
The brutal ruler of the tiny nation of Latveria, Dr. Doom is the archfoe of the Fantastic Four, and a major antagonist during the Secret Wars arc. Combining local technology with his own genius, he carves out an empire for himself, and plots to steal the Beyonder's powers for his own.
A God Am I: Plans to steal the powers of the Beyonder and partially succeeds.
Pragmatic Villainy / Dangerously Genre Savvy: While all of the other villains are busy playing Evil Overlord and turning their portions of the planet they find themselves on into brutal crapsackempires, feuding over their land like mad dogs, Doom spends his time wisely: Doom easily creates a utopia for his own isolated corner of the world where his subjects actually want to worship him because he saved them from the other villains, and makes himself out to be a Big Good. When the heroes show up, rather than start a fight with them he uses traps, trickery and false promises the either remove or totally placate them before they become problems. If not for attempting to bite off more than he could chew by stealing the Beyonder's power, the heroes might never have been able to wrest control from him.
A Spider-Man from a world where Uncle Ben didn't die, the public accepts him, J. Jonah Jameson is his godfather, and he's a multi-billionaire. Also a raging ego trip. He also made a terrible choice picking his lawyer.
Jerkass: He's completely insensitive, acts like he's God's gift to the world, and has no regard for his teammates' lives. Most of this stems from never having lost before.
Jerk with a Heart of Gold: Whatever else you might say about him, he's still a superhero, is still dedicated to doing the right thing, and honestly seems to care about his world and his girlfriend, Gwen Stacy.
Like the main universe Spider-Man, this Spider-Man suffered from a mutation that gave him four his extra arms. Instead of trying to undo the mutation he decided on giving them webshooters. However, when he joined the Spider-Men he mutated further into the Man Spider beast, attacking the others. He was returned to his own dimension and as such unable to help the others any further.
This Spider-Man is a completely powerless normal human, and as such has no powers of his own. In his world Spider-Man is a creation of Stan Lee. Though the other Spider-Men asked him to stay behind, he was later able to help stop the destruction of the multiverse.
"Ever since that spider bit me, the world has misunderstood me and tormented me. But now, it's my turn. I'm gonna obliterate you all!"
The Spider-Man from the Scarlet Spider's reality, he lost both Aunt May and Uncle Ben at an early age. His rivalry with the Scarlet Spider (whom he believed to be his clone) only worsened his tenuous grip on reality, and the revelation that he himself might actually be the clone drove him into a homicidal rage. Following his subsequent possession by the Carnage symbiote he now actively seeks to end all life in the multiverse.
Apocalypse How: Class Z. Plans to combine portal technology with explosives to destroy not only Earth, not only the universe, but the totality of reality. When this plan fails he rants that he will destroy every universe in existence if he has to do it one dimension at a time.
Driven to Suicide/Heroic Sacrifice: Undergoes a Heel Realisation, comes to the conclusion that he cannot fight the symbiote off, and rather than letting it take control of him again, decides that suicide is the best option, and disintegrates himself by leaping into an unstable vortex.
The Mentally Ill: Suffers from self-loathing, misplaced envy, and suicidal depression, coupled with aggression issues and a need to lash out at the world. It isn't pretty and is played enitrely for tragedy.