Dude, Where's My Respect?: All the time. He's constantly targeted by people who think he's a villain, the public hates him, and he gets no respect in his civillian life either.
Hero with Bad Publicity: It isn't as extreme as in some of the comics, but yeah. The Daily Bugle is far from TAS Spider-Man's best friend.
With Great Power Comes Great Responsibility: Said word for word almost every other episode. It becomes a plot point during the confrontations with Doctor Doom and Spider-Carnage.
Peter's first Love Interest on the show, Felicia's life becomes a lot more interesting after The Kingpin uses her as a test subject in an experiment involving Super SoldierSuper Serum. She becomes the Black Cat, a vigilante who is a frequent ally to Spider-Man.
A friend of both Peter and Spider-Man, Connors is the victim of a failed experiment that causes him to change into a human/lizard hybrid at various intervals.
Well-Intentioned Extremist: The Lizard was one in his first appearance, believing that people would be better off as reptiles.
Harry Osborn/Green Goblin II
A classmate of Peter's, Harry eventually becomes his roomate and closest friend. Unfortunately, his breakup with Mary-Jane and subsequent jealousy over her relationship with Peter, coupled with the revelation that his father, Norman, was the original Green Goblin unbalance his mind, leading him to adopt the identity for himself.
Love Redeems: Liz certainly hopes so. Given that his problems can be boiled down to "nobody loves me" she has a shot.
The Mentally Ill: He suffers from persecution paranoia, feelings of isolation, and a serious daddy issues. As in Norman's case it is played seriously and tragically.
My Significance Sense Is Tingling: At the end of his guest appearance, he notices that his actions were being watched by a powerful entity (Madame Web).
Tony Stark/Iron Man
A billionaire scientist with a double-identity, Iron Man aids Spider-Man against Venom and Carnage when they try to rob one of his labs.
A blind attorney who represents Peter during his trial for treason, Matt Murdock's father was murdered on orders from The Kingpin. He maintains a doublelife as the vigilante, Daredevil, and helps Spider-Man discover the truth about The Kingpin's identity.
A WWII supersoldier, and leader of the Six American Warriors, he was trapped in a vortex alongside his archnemesis The Red Skull at the end of the war.
Plucky Girl: She takes no crap from her egomaniac boyfriend, realises that Spider-Carnage is a fake and saves our Spider-Man, and then helps drive off Spider-Carnage by opening up on him with a sonic rifle. If it weren't for her kidnapping and five minute appearance she might be a full-on Action Girl.
Spot The Impostor: Instantly recognises Spider-Carnage as a fake. She also seems to have figured out that our Spider-Man isn't hers, although she still recognises him as a hero.
Spider-Man: How'd you know that the Peter Parker downstairs wasn't me?
Gwen Stacy: He's as nutty as a fruitcake. A girlfriend notices little things like that.
Enemies
Wilson Fisk/The Kingpin
The main villain of the series, the Kingpin operates a criminal empire with its headquarters in New York City. He is The Man Behind the Man to many of the villains in the series, including all the incarnations of the Insidious Six and the Spider-Slayers.
Amoral Attorney: Not our Kingpin (who pretends to be a businessman and philanthropist) but the version of Fisk in the Iron Spider-Man's world definitely qualifies.
Badass Normal: Bordering on Charles Atlas Superpower. This is an unpowered human who can swallow blows from Spider-Man and topple giant robots with only his raw brute strength.
The Man Behind the Man: He's the Big Bad to the Insidious Six, the Crime Cartel, and almost every other major supervillain on the show, but he keeps to the shadows and Spider-Man does not meet him face-to-face until the third season.
Take Over the World: Would love to do so, although he's fairly practical about it, focussing more on spreading his influence than crazed schemes—although he certainly has a few of those up his sleeves.
Well Done Son Guy: In the backstory. He is pleased that his relationship with his son is nothing like the one he had with his father.
Robot Master: Built the original Black Widow Spider-Slayer.
Alistair Smythe
Spencer Smythe's wheelchair-bound son, Alistair becomes The Kingpin's right-hand man for much of the show, serving as his chief inventor and confidante. He is later transformed into a living Spider-Slayer by The Kingpin and Landon.
Antivillain: At first he's being played by The Kingpin; later he's trying to save his father.
Ascended Extra: In the comics Alistair is a fairly minor villain. In TAS he's an Evil Genius-for-hire whose inventions play a vital role in the schemes of half the crimelords in New York.
The Evil Genius: Fills this role for The Kingpin, Silvermane, Harry Osborn, The Red Skull, and anyone else who will fund his attempts to save his father.
Evil Sounds Deep: Alistair's voice is already pretty low. As the Ultimate Slayer it drops another octave and gains mechanical distortion.
Love Makes You Evil: In a non-romantic sense. His entire criminal career is motivated first by revenge and later by the desire to save his father Spencer.
A fanatical scientist with an anti-mutant agenda, he is later transformed into a freak of nature by one of his own experiments. He eventually replaces Alistair Smythe as The Kingpin's Number Two.
Fantastic Racism: Towards mutants, including his old partner, Hank McCoy.
Final Solution: His chemical concoction destroys mutant cells, ultimately killing the victims. He plans to use it on every mutant in the world, killing them all.
Gone Horribly Wrong: Exposure to his own chemicals transforms him into one of the very mutants he so despises.
Once a respected scientist, Dr. Otto Octavius was Peter Parker's mentor as science camp when he was ten. Following an accident that bonded his tentacles to his body, Octavius has become a criminal mastermind, though he still has a soft spot for his former students. He is a core member of the Insidious Six and acts as their field leader.
Adaptational Attractiveness: Octavius in the comics is a fat, ugly man with a bad haircut. This version retains the bad haircut and square face but has the physique of a body builder.
Affably Evil: Particularly towards Peter and Aunt May.
Big Bad: In a Five-Bad Band sense. He acts as the leader of the Insidious Six whenver they are recruited by The Kingpin. This arguably makes him Co Dragons to The Kingpin alongside Smythe or Landon.
A former private investigator who went mad after Jameson had him transformed into a weapon to use against Spider-Man. He alternates between working as a mercenary for The Kingpin and other crime lords (most notably Silvermane) and wanting to return to normal. A member of both incarnations of the Insidious Six.
The Brute: Shares this role with The Rhino as a member of the Insidious Six.
Chronic Villainy: No matter how hard he tries, or what he promises Sarah, Mac just can't seem to keep out of the underworld, ineveitably falling back into crime whenever he is short of cash.
Dumb Muscle: He's not out and out stupid like The Rhino, but Mac is still hot-tempered, impulsive, and dangerously short-sighted.
Even Bad Men Love Their Mamas: Yells at Spider-Man about not dragging his mother into things when the latter cracks a "your mom" joke.
Hero Killer: Subverted. He's dangerous certainly, but despite the buildup, isn't significantly more threatening than other members of the Six (and less so than some).
"You should have stayed in black, because I'm gonna dirty you up bad."
A supervillain-for-hire his motivations and past are never explained. He frequently works for The Kingpin and provides the Insidious Six with their heaviest muscle.
"This time it's maximum voltage at minimum range."
A mercenary in The Kingpin's employ, Shocker fires vibrational waves from his gauntlets. He is a member of the Insidious Six and tangles with Spider-Man as part of the group and on his own.
Achilles Heel: Downplayed. Water can cause his gauntlets to short out, but it's only a serious problem for him if his insulated suit is also damaged. This convenient allignment of factors happens once in the entire show.
Adaptational Badass: Shocker in the comics is treated as a joke, regardless of his (not unimpressive) track record. This version managed to give the black-suited Spider-Man a run for his money and is given the appropriate amount of respect.
Clothes Make the Superman: His insulated suit allows him to channel his attacks and conduct electricity safely.
Evil Duo: Forms one with The Rhino in one episode. During the "Forgotten Warriors" arc he and Scorpion form one within the Insidious Six. In both cases, he's the calm and collected one.
Unholy Matrimony: Eventually develops a crush on a badly disfigured (and totally unhinged) actress.
Adrian Toomes/The Vulture
A failed businessman with a grudge against Norman Osborn, the Vulture is an old man who eventually develops a way to feed off of his victims' youth in order to temporarily become young again. By stealing the rejuvenated Silvermane's youth he is able to make the process permanent; he then joins the Insidious Six as Mysterio's replacement.
Shapeshifter Mode Lock: For a while his power was on the fritz, causing him to shift between old and young at the drop of a hat. He eventually managed to freeze himself at young by stealing Silvermane's newly accquired youth.
A nameless, faceless, shapeshifting spy, found most often in The Kingpin's employ. He is later revealed to be The Red Skull's foster son and his mole within The Kingpin's organisation.
"From now on, we're poison to you Spider-Man! That's why we call ourselves VENOM!"
A washed-out Daily Bugle reporter turned violent criminal, Brock becomes Venom after bonding with the alien symbiote that was once a part of Spider-Man.
From Nobody to Nightmare: Went from a lame reporter who couldn't even pay his own rent to a frightening badass capable of easily overpowering Spider-Man in a fight.
A criminal lunatic, Kasady had the cell next to Eddie Brock. Baron Mordo offered him the chance to gain the same kind of power that Brock had, granting him one of Venom's offspring as a gift and transforming him into Carnage.
Badass Abnormal: Cletus Kasady held off the NYPD and Spider-Man using his own cunning and liberal doses of high-explosives. Needless to say, he becomes an even serious problem after he turns into Carnage and completely averts From Nobody to Nightmare. When it's discovered that it's Kasady behind Carnage's mask, everyone becomes more frightened.
Omnicidal Maniac: His loyalty to Dormammu appears to be less about gratitude for the symbiote and more because he knows exactly what will happen when Dormammu is freed from the Dark Dimension.
Shape Shifter Weapon: Frequently stretches out his symbiote or turns his hands into weapons.
Slasher Smile: Frequently shows one as Kasady; gains a permanent one as Carnage.
The Sociopath: The clearest-cut example on the show. Kasady likes to hurt people, has a Hair-Trigger Temper, expresses no remorse for his actions, and has no regard whatsoever for human life, his own included.
A killer created and retained by Norman Osborn for the purposes of assassinating The Kingpin, the Hobgoblin eventually turned freelance criminal. Who was behind his mask remained a mystery for much of the show; it was eventually revealed to be Jason Phillips, Felicia Hardy's fiance.
Chronic Backstabbing Disorder: He backstabs Norman Osborn, The Kingpin, Landon, and anyone else who crosses his path over the course of the show. Special note should go to his introductory two-parter. He doesn't just double-cross, he doesn't just triple-cross, he goes for the very rare quadruple-cross.
From Nobody to Nightmare: He was a petty street thug and legbreaker before Osborn got his hands on him. Now he's a Psycho for Hire who is a genuine threat to the likes of Spider-Man and The Kingpin.
Gold Digger: Plans to marry Felicia for her money.
The father of Peter's friend Harry, Norman is a crooked businessman who starts the show almost completely under the thumb of The Kingpin. A lab accident eventually results in him developing a split personality; donning an altered version of the Hobgoblin's gear he becomes the Green Goblin and seeks revenge on everyone who has ever made his life difficult.
Antivillain: Norman's a fairly nice, if weak-willed guy who's in way over his head with The Kingpin. Even the Green Goblin persona can be seen as something of a Knight Templar rather than the Axe Crazy loon he's normally portrayed as.
Split Personality: Big time. The Green Goblin persona in this version of the story is a protector personality who seeks to protect Norman from anyone who has ever done him wrong.
A member of the Crime Cartel and a rival of Wilson Fisk's, Silvermane is governed by two obsessions—to seize control of the cartel from The Kingpin, and to regain his lost youth.
Be Careful What You Wish For: Wished to be young again. Was turned into a baby. He then wished to regain his adult body—and was aged to an even older state than beforehand by The Vulture. Feel free to laugh.
Butt Monkey: He gets screwed over by Fisk, The Scorpion, and The Vulture (not to mention Spider-Man), and all of his schemes backfire horribly. You'd feel sorry for him if he wasn't such a dick.
Charles Atlas Superpower: When he regains his youth he's able to beat up The Lizard with his bare hands. Somehow.
A childhood friend of Robbie Robertson's, Lonnie Lincoln was forever changed following a mishap at a chemical plant. He now operates as an underworld hitman, taking jobs for the likes of Silvermane and Richard Fisk.
Belated Backstory: Appears in Season 2 as a hired thug working for Silvermane. Doesn't get any backstory until Season 3, when his identity, origin, and vendettta against Robbie are all established.
Revenge: When they were kids, Robbie left Lonnie in the lurch following a corner store robbery; Lonnie went to juvie. As an adult he tried to frame Robbie for a crime he didn't commit in order to get back at him.
Revenge by Proxy: Following his transformation into Tombstone, Lonnie tries to frame Robbie's son in order to get back at him.
Super Strength: Easily in the running for strongest character in the show.
An ex-boyfriend of Mary-Jane's who later gained the power to manipulate water. Defeated by Spider-Man he evaporates; a clone of him later returns to cause havoc in his place.
Unwitting Pawn: The clone is an unwitting pawn of Miles Warren.
Baron Mordo
An evil sorcerer with ties to the netherworld, Baron Mordo works tirelessly to free his master, the dread Dormammu from his prison. To that end he has brainwashed innocent people, stolen souls, and even conspired with Venom and Carnage.
A biologist with a penchant for clones, Warren causes a lot of trouble in both our Spider-Man's world, and the alternate reality belonging to Spider-Carnage and the Scarlet Spider.
Karma Houdini: Our Warren never recieves any commupance; whether the alternate!Warren perished in the apocalypse triggered by Spider-Carnage is left unanswered.
The son of the Red Skull, Schmidt spent most of his life trying to free his father from the vortex. Manipulating The Kingpin and Silver Sable into doing his bidding, Schmidt ultimately freed his father who "rewarded" him by transforming him into his ultimate weapon, Electro.
Adaptational Badass: Big time. He brings down all of SHEILD with a gesture, and is too powerful for Spider-Man to confront directly.
"Do not despair Chameleon. The Red Skull anticipates everything."
A Nazi spymaster, and archfoe of Captain America and the Six American Warriors, The Red Skull was trapped in the vortex alongside Cap. He is eventually released by his son, Rheinholt, whom he transforms into Electro; following Electro's betrayal, he and Cap are once again trapped in the vortex.
"This is what I have always wanted—absolute power and control!"
The brutal ruler of the tiny nation of Latveria, Dr. Doom is the archfoe of the Fantastic Four, and a major antagonist during the Secret Wars arc. Combining local technology with his own genius, he carves out an empire for himself, and plots to steal the Beyonder's powers for his own.
A God Am I: Plans to steal the powers of the Beyonder and partially succeeds.
Power Incontinence: After seizing control of the Beyonder's power. As smart as he is, Doom simply isn't equipped to control the strength of a veritable god.
A Spider-Man from a world where Uncle Ben didn't die, the public accepts him, J. Jonah Jameson is his godfather, and he's a multi-billionaire. Also a raging ego trip.
Jerkass: He's completely insensitive, acts like he's God's gift to the world, and has no regard for his teammates' lives. Most of this stems from never having lost before.
Jerk with a Heart of Gold: Whatever else you might say about him, he's still a superhero, is still dedicated to doing the right thing, and honestly seems to care about his world and his girlfriend, Gwen Stacy.
"My name is Ben Reilly. I'm a clone of my world's Peter Parker. Or maybe he's a clone of me. We're not really sure."
A clone of his universe's Spider-Man (maybe) created by Doctor Miles Warren, the Scarlet Spider changed his name to Ben Reilly and pursued his own career as a superhero.
"Ever since that spider bit me, the world has misunderstood me and tormented me. But now, it's my turn. I'm gonna obliterate you all!"
The Spider-Man from the Scarlet Spider's reality, he lost both Aunt May and Uncle Ben at an early age. His rivalry with the Scarlet Spider (whom he believed to be his clone) only worsened his tenuous grip on reality, and the revelation that he himself might actually be the clone drove him into a homicidal rage. Following his subsequent possession by the Carnage symbiote he now actively seeks to end all life in the multiverse.
Apocalypse How: Class Z. Plans to combine portal technology with explosives to destroy not only earth, not only the universe, but the totality of reality. When this plan fails he rants that he will destroy every universe in existence if he has to do it one dimension at a time.
Beta Test Baddie: Whether he's the clone or not, Spider-Carnage clearly feels deeply insecure about his identity, which only contributes to his mental instability.
Driven to Suicide/Heroic Sacrifice: Undergoes a Heel Realisation, comes to the conclusion that he cannot fight the symbiote off, and rather than letting it take control of him again, decides that suicide is the best option, and disintegrates himself by leaping into an unstable vortex.
The Mentally Ill: Suffers from self-loathing, misplaced envy, and suicidal depression, coupled with aggression issues and a need to lash out at the world. It isn't pretty and is played enitrely for tragedy.
Redemption Equals Death: Realises what he has become, and kills himself and the symbiote rather than letting it go through with the plan.
Shapeshifter Weapon: Like the original Carnage, he's able to morph his symbiote into a wide variety of weaponry, including extendable claws, Combat Tentacles, axes, and a shield.
Spot The Impostor: According to Gwen Stacy this was easy. "He's nutty as a fruitcake."
With Great Power Comes Great Insanity: The poster boy. His Spider-powers, coupled with his already severe emotional fragility and the Carnage symbiote have pushed him totally around the bend.