Amazonian Beauty: Started out on the slender side, but was continually beefedup as the series progressed. Of course this makes sense considering the amount of exercise she'd get fighting supervillains and bounding across New York.
Everyone Loves Blondes: Four of May's love interests (Brad, J.J., Franklin and Gene) are blondes. Two are evil.
Expository Hairstyle Change: Changing her short Tomboy look for longer locks in Amazing Spider-Girl. Notably the haircut that stuck.
Expy: Played with but ultimately averted. May has similarities to Peter, MJ and even Ben (Peter lampshades that one) but is her own character. Word Of God is that this was deliberate.
Foe Yay: With Normie and Black Tarantula. The latter wants to her to bear his heirs, the former... let's just say the Venom/Spider-Girl was dripping with UST.
Hollywood Nerd: Looks like a Brainy Brunette version of Mary Jane, knows a few things about science, and openly fangirls superheroes.
Important Haircut: Averted. May kept changing her hairstyle every 10 or so issues just because. Until she finally settled on a look in Amazing Spider-Girl
Uncanny Family Resemblance: Played straight at first, as she was drawn as a female Peter, but averted as the art evolved. Now she resembles both her parents equally.
Hollywood Nerd: Averted and played straight. She doesn't have the body type, but she does have the hobbies, and the boys (especially Moose) seem to like her anyway.
Chess Master / Manipulative Bastard: Manages to get and keep the Kingpin's criminal empire after the latter's death. Which he arranged, by the way. At least until he starts losing it all in Spectacular.
Mama Bear: Granted, an evil and abusive version, but still.
Roaring Rampage of Revenge: Goes on one following her elder son's death. Somewhat subverted in that she and her surviving son tear a swath through the underground but never get to the people responsible.
Strait Jacket: Kept hers on after her son broke her out.
You Killed My Father You Killed My Son: Blames Spider-Girl for Crazy Eight's death.
Carnage ( Moose Mansfield & Benjy Parker)
Body Horror: For Moose. And who knows how it affected Benjy...
Crazy Eight
Affably Evil: He's funny, calls his mother and looks out for his little brother. When they're not trying to kill each other. He even compliments a rookie Spider-Girl on her fighting skills!
Clothes Make the Legend: Seems to think so, since he refuses to wear normal clothes to his trial for the sake of his reputation. Played heartbreakingly straight after his death when we finally see him without the makeup... as he's being buried in a nondescript suit.
Never My Fault: His brother's death might not have happened if he hadn't exacerbated tensions between the Kingpin and Canis out of sibling rivalry. Naturally, he blames Spider-Girl for it instead.