Space Colony features a cast of 19 crew members, though not all appear at once in the colony. They carry with them a wide variety of tropes of their own:
Venus JonesA Coloradan woman who serves as the team leader.
Stig SvensonA Norwegian biker brought on to help operate heavy machinery.
Tami La BelleA Texan woman brought in to aid with cleaning duties.
Dean Jefferson BrownA Californian man brought in as the team doctor.
Daisy WillowreedA Californian woman brought in to help with the colony's botany.
Billy Bob PerkinsAn American man from the Deep South brought in to help run the colony's space chicken farming operation.
Candy SimpsonA Valley Girl brought in to — well, do janitorial work and learn something.
Captain Charles WilmingtonA former British navyman kicked out of the Navy for some unknown offense, which he says was a "silly incident". He is brought in to help man the turrets.
Greg ChestertonA Jerkass extroardinaire who's high maintenance and mixed in skills.
Hoshi and KitaTwo Japanese twins brought in to help with general purpose work and operations.
Barbara LeechworthA former Vice President of Sales at Blackwater Industries, brought in to help the colony's general operations.
'Nailer' McBrideA Violent Glaswegian brought in to help operate the team's heavy machinery and turret guns.
Nikolai VolkovA Russian man brought in to aid the team's biochemical and scientific endeavors.
Ashia GreenA British woman and former athlete brought in after an injury ended her career. She is still quite athletic, being known as the sporty one of the team. She aids with a wide variety of duties, including turret operation.
Babette DevereuxA snobby French woman brought in for similar reasons as Candy.
Vasilios KosmosA Greek man who was the victim of some unknown psychic attack (possibly from the Brainspawn), that rendered him emotionless and a Cloudcuckoolander. He starts with few skills, but is a good learner.
SlimA middle class white New York City boy pretending to be a lower class black boy and gangster. An avid rap fan who begins with few skills but has the big advantage of using his skateboard to get around the base rather than walking, greatly addding to his speed.
Bhoomi SharmaAn old Indian woman brought in as the team janitor and for moral support.
Mr. ZhangA Chinese Grumpy Old Man and old robotics specialist who minors in botany.