Simone Lenoir and Lena Dupree
Simone Lenoir: Adrienne Barbeau
Lena Dupree: Tara Strong
- Anti-Villain: It's understandable for Simone and Lena to be upset with Moonstar and want revenge against their people's deaths. However, since they take innocent people's lives to fuel their own, that qualifies them as the real villains.
- Berserk Button: Dogs, for Simone. They chase her cats, after all, and she is in fact a cat.
- Big Bad: Simone.
- Blondes Are Evil: Simone.
- Cat Girl: Simone and Lena are revealed to be a particularly horrifying◊ variation.
- Deal with the Devil: Cut a deal with a cat god for immortality.
- The Dragon: Lena.
- Family-Unfriendly Death: See No Immortal Inertia below.
- Genre Blindness: They thought so little of Shaggy and Scooby they did not even bother making voodoo dolls of them. This comes back to bite them later.
- Jerkass: Simone can come across as this.
- Kick the Dog/Kick the Son of a Bitch: Simone tells Lena to abandon Jacques after the zombies dogpile him.
- Living Forever Is Awesome: They weren't trying to, but seem to just go with it.
- No Immortal Inertia: The gang succeeds in stalling Simone, Lena, and Jacques long enough for the deadline by which they need to steal souls to pass, and they promptly age into dust.
- Pet the Dog: Lena is the least evil of the three, and is shown to care for the others, unlike Simone who would gladly sacrifice her henchmen toward the completion of her goal, and Jacques who is essentially a thug.
- Really 700 Years Old: They were there when Morgan Moonstar and his crew invaded the island and killed off their friends and family. They've been living off the essence of any poor soul they lure to their island.
- Redemption Rejection: Simone and Lena did not have to keep draining people's life energy, with the pirates dead, to preserve their immortality. They could've chosen to just let themselves die (as they would have anyway, of old age, if they never invoked the cat god's power), but they CHOSE to still feed off people, even after they'd gotten their revenge against the pirates. As for Jacques, even though he asked them for it, they could have refused him but they didn't.
- Roaring Rampage of Revenge: The reason for the aforementioned cat god deal.
- Romantic False Lead: Fred falls for Lena at their first meeting.
- Southern Belle: Lena's not wealthy, but she has shades of this.
- Start of Darkness: Originally, they were part of a group of settlers who lived on the island and worshipped the cat god. During the harvest moon, when they were celebrating a successful harvest, Moonscar and his pirates arrived on the island. To keep the location of their stolen treasure secret, Moonscar and his pirates chased the settlers into the bayou where they were eaten by alligators (except Simone and Lena, who had left when the pirates arrived and returned just in time to watch everyone else die). They begged their cat god to curse the pirates, and their wish was granted, turning Simone and Lena into werecats who then killed them. However, they had to drain the life force from other people every harvest moon to preserve their immortality.
- He Who Fights Monsters: What Moonscar did to Lena and Simone's people was evil, yes. But what the two do after they get their revenge made them much more monstrous (literal and figuratively) then he ever was.
- Woobie, Destroyer of Worlds: You will want to give Lena a big hug after hearing her and Simone's Start of Darkness. Simone to a lesser extent, only because she'd likely claw your eyes out if you tried.
The Zombies from Zombie IslandThe Zombies From Zombie Island. They're not actually evil, they're just unrested souls trying to warn the gang of the real villains on the island; Simone, Lena, and Jacques.
- And I Must Scream: They are unable to return to the afterlife...at least until the Mystery Gang frees them.
- Asshole Victim: The pirates in the Backstory who were turned into zombies. Not the other people (such as the Civil War soldiers or the tourists turned into zombies), technically.
- Dark Is Not Evil / Good All Along: They just wanted to scare the gang off to save them from the same fate that occured to them.
- Becoming the Mask: He takes the gardening job very seriously, even reacting with genuine concern when Scooby accidentally ruins the work he put into it.
- Fire-Forged Friends: With Velma. She thinks Beau's a suspect, but eventually warms up to him.
- Red Herring: Beau to make it look like another criminal in a mask. Not only is that not the case, but he's an undercover cop.
- Reverse Mole: Beau is actually investigating the island's disappearances.
- Romantic False Lead: For Daphne.
- Scary Minority Suspect: Beau isn't a minority, but he fits nearly every other criterion.
- Bitch in Sheep's Clothing: He seems like a nice guy, but it ultimately turns out that of the three, he is the worst because he actually asked for the curse.
- The Brute: To Simone's Big Bad and Lena's Dragon.
- Living Forever Is Awesome: Unlike Lena and Simone, he actually asks for it.
- And I Must Scream: Implied to be his final fate.
- Big Bad Wannabe: No, he's not a better magician than his ancestor.
- Even Evil Has Standards: He freed his ancestor to rule the world together. But she wants to destroy it as revenge for her imprisonment.
- Expy: Apparently one for Stephen King, though he's probably named for HP Lovecraft.
- Genre Blindness: If he hadn't suddenly grabbed the Idiot Ball and pissed off the ghost, he probably would've won.
- Romantic False Lead: Velma falls for him hard.
- The Cloud Cuckoolander Was Right: He was indeed an abductee. By the fakes.
- Exact Words: He claims to have pictures of the aliens who got him. He does. He painted them himself.
Crystal and Amber
- But Now I Must Go: Though they leave on friendly terms, Shaggy and Scooby are still heartbroken.
- Defrosting Ice Queen: Amber is initially cold to Scooby's advances, but gradually warms up to him.
- New-Age Retro Hippie: Crystal is one. Or so it seems, until it's revealed that she's an Alien Among Us—and so is Amber.
- Talking Animal: Amber turns out to be one, which surprises Shaggy and Scooby. Fred doesn't let this go unlampshaded.
Steve, Laura and Max
- Affably Evil: Particularly Max when he first meets the group.
- Big Bad: They're the so called "invaders", using alien disguises (with a movie prop to simulate an alien spacecraft) to scare away anyone from the mines so they can dig up the gold. At the same time keeping their cushy jobs. This wouldn't seem so bad until they nearly try to kill Fred, Velma and Daphne to keep their secret hidden.
- Surveillance Station Slacker
BuckGruff vehicle repairman. Works at his own pace and can often be seen on the desert roads.
- Jerkass Fašade: He seems rough, but he only does so so to keep people from thinking he's a softie.
- Real Men Wear Pink: He collects pressed flowers.
Dottie and Sergio
- Abhorrent Admirer: Dottie for Fred. Sergio is quick to point this out.
MP OfficersA pair of government officers stationed to prevent trespassing.
The Phantom Virus
- A.I. Is a Crapshoot: Not to Bill, though.
- Big Bad: Of Scooby-Doo and the Cyber Chase.
- Card-Carrying Villain: Probably doesn't know why he even exists, but damn, he seems to be happy as long as he gets to make misery for the gang.
- Incredibly Lame Pun: Constantly spewing out new baseball related quips, which ultimately clues the gang in to who created him.
- Killed Off for Real: After being beaten, he's deleted as the game starts to reset itself.
- Kryptonite Factor: His reaction to Magnets.
- Person of Mass Destruction: As pointed out, if allowed to run loose in the real world, he could potentially destroy civilization.
- Technopath: He demonstrates the ability to control machines.
- Green-Eyed Monster: The reason why he created the phantom virus.
- The Man Behind the Man: He created the Phantom Virus.
- Agent Mulder: He believes in the Loch Ness Monster, and insists that the highland games are angering it, and that it needs protection. Once "Nessie was a no-show", he moves up to the Himilayas to search for the Abominable Snowman.
- Continuity Nod: He gives up on Nessie and moved to the Himalayas where he became the mountain's DJ much to Shaggy and Scooby's happy surprise.
- Cool Car: His van, which was essential in the Scooby-Doo Hoax after it was stolen and used for one of the land Loch Ness Monsters.
- Meaningful Name: Chillman.
The Goblin King
- Papa Wolf: He would've traded his Goblin Scepter just so his daughter would be safe.
Fairy Princess Willow
- Dumb Blonde: While there's really not enough information to justify this, she was messing with the amazing Krudsky, and eventually got caught, leading to his rise to power and the almost downfall of the world being entirely her fault.
- The Voiceless: Until the climax (surprising considering Panettiere is listed higher in the credits than any of the non-regulars).
Glob and Glum
James Belushi: Glob
Larry Joe Campbell: Glum
- Card-Carrying Villain: They appear as antagonists, but they're working for the Goblin King, who's not actually evil. They probably don't know this, though.
- Adorkable: When she tries to get Shaggy to notice her
The Woodsman, the Fishman, and the Specter of Shadow Canyon
- Ax-Crazy: The Woodsman.
- Chekhov's Skill: Knudsen using the zipline to dress up as the banshee.
- Family-Unfriendly Death: The Woodsman's backstory at the beginning of the movie.
- Fish People: The Fishman.
- Our Banshees Are Louder: The Specter of Shadow Canyon.
- Red Eyes, Take Warning: The Specter.
- Shark Man: The Fishman again.
- Scooby-Doo Hoax: The thing was staged by Deacon/Babyface Boretti and Ranger Knudsen so that the campers would leave and they could blow up the dam directly over Camp Little Moose without killing anybody. Once they were gone, the empty lake at Camp Big Moose would reveal a long lost town, where they could search for a treasure buried by Boretti's lost colleague.
- Action Girl: To an extent.
- Brainy Brunette: She definitely is a pretty smart girl.
- Impossible Hourglass Figure
- Ms. Fanservice: Possibly even gives Daphne a run for her money.
- Adults Are Useless: Every time the gang tries to report something to him, he doesn't really care. Probably because he didn't want them getting suspicious.
- Affably Evil: He flirts with Velma, and even tries to clear everybody out of the camp before blowing the dam and flooding the place.
- Ax-Crazy: As the Woodsman.
- Darker and Edgier: Its obvious that this film is much Darker and Edgier than previous ones, especially this villain, who swung his axe like he was giving out candy.
- Even Evil Has Standards: Still tried evacuating the camp before flooding it.
- Stalker with a Crush: Can be interpreted as this towards Velma.
Deacon Babyface Boretti
- Big Bad: Think about it. Between him and Knudsen, who's idea was it to evacuate the camp before flooding it?
- Even Evil Has Standards: Still tried evacuating the camp before flooding it.
- Nerd Glasses: Pretty much his entire disguise, besides being dressed as a camper.
- Nerdy Inhaler: As part of his disguise, he pretending to have asthma.
The PhantosaurThe main antagonist of Scooby-Doo! Legend of the Phantosaur. Has two forms; the original form is a large, glowing, tangible dinosaur, and the second form is made completely of fire, also with the ability to breathe fire.
- Dinosaurs Are Dragons: The Fiery Phantosaur can breathe fire, in addition to being made of it.
- Everything's Better with Dinosaurs
- Man in the Machine: The original Phantosaur was being robotically controlled. Averted with the Fiery Phantosaur, which was made using computer graphics and a flamethrower.
- Playing with Fire: The Fiery Phantosaur.
- Scooby-Doo Hoax: The original people wanted to scare people away for land or something. But their not the people you remember. Professor Svankmajer and Winsor wanted to scare the whole town away so that they could safely remove, and keep for themselves, a perfectly preserved dinosuar, buried in crystals.
- Blondes Are Evil: She is blonde and has part in the crime.
- Hot Scientist: She is a professor of paleontology.
- Badass Bookworm: Not necessarily a book worm, but the computer animation classes he'd taken definitely come in handy.
- Geeky Turn-On: He and Velma were instantly attracted when they met at the dig. They discussed dinosaur fossils and discovered they had a lot in common.
- Hollywood Nerd: No surprise considering that is a male version of Velma.
- Romantic False Lead: To Velma. Though their last conversation may suggest otherwise.
- Cloudcuckoolander: He seems distracted; he can't get Shaggy's nickname right, for example.
- Hypno Fool: His hypnotism equipment works so well, that he always hypnotizing himself at the end. Not throughout the whole thing, just at the part where he tells his subject to wake up having no recollection of what happened.
- Nice Job Breaking It, Hero: At the end, Shaggy's hypnosis is undone, only for everyone else in the gang to believe their Shaggy as well. From what we see at the ending, Mr. Hubley doesn't fix it.
- Recognition Failure: He never gets Shaggy's name right.
- Red Herring: He's basically just there so that there's more suspects, but he had pretty much nothing to do with the Phantosaur attacks.
- Klatchian Coffee: That's his business. But by the looks of it, he drinks quite a bit as well.
- Red Herring: He's one of the suspects, and his behavior is a little suspicious, but Archambault was just trying to frame him.
- Big Bad: Of Big Top.
- Dumb Muscle: Averted. He's very intelligent. After all, he was probably the closest villain to getting away with it if it weren't for Velma figuring out that Doubleday wasn't working alone.
- Kick the Dog: "Archambault never punch a horse before. Could be fun!"
Oliverio and Lena
Oliverio: Carlos Ferro
Lena: Hynden Walch
- Crazy Jealous Guy: Oliverio of Fred when Lena flirt with him.
- Impossible Hourglass Figure: Lena has an athletic build.
- Mother Russia Makes You Strong: Though, they're supposed to be German.
- Blondes Are Evil: He conspired with Archambault to carry out jewel robberies, using werewolf-disguised baboons.
- Flat Character: Other than him appearing in one scene at the beginning before going missing and helping Archambault with his plan what's to this guy?
- Hey, It's That Voice!: Craig Ferguson, host of CBS' Late Late Show.
- Scooby-Doo Hoax: Works with Archambault to kidnap the circus stars, ruin the show, and takeover from Marius since they were jealous that his father gave the circus to him instead of them after they had worked there longer than Marius.
Wulfric von Rydingsvard
- Red Herring: Everywhere his band traveled there have been werewolf attacks. That's because they wanted the black daimond he wears.