Helen: I have standards, Will, and drinking coffee? Far below them.
Omnidisciplinary Scientist: Justified, since she has had 157 years to study and has been surrounded from birth by the world's leading scientists. Even then, she has mainly focused on the biology and chemistry of Abnormals, rather than any of the social sciences (which is why she hires Will) or the high technology that the Sanctuary uses (which is why she hired Henry), and she seems to only dabble in the physics behind Abnormals.
Really 700 Years Old: She 157 years old when the series starts but is physically in her 40s. Later she has to take The Slow Path, so it's more like 260, but still no noticeable change in appearance, so it would seem Helen has truly become The Ageless.
Ashley Magnus (Emilie Ullerup)
Will Zimmerman (Robin Dunne)
Chickification: Will Zimmerman is a rare male example. He's a brilliant forensic investigator, an emotionally tough, tenacious maverick willing to find the truth at any price... in the pilot. In subsequent episodes, he becomes the Designated Victim, losing his glasses and (frequently) 50% of his pilot IQ and personality in the process, as well as gaining tighter clothes and lots of hair product. As of the fourth season premiere, he seems to be getting better.
Future Badass: In "Pavor Nocturnus". His actions even scare the crap of Magnus of all people.
Identical Grandson: Captain Jack Zimmerman, Will's identical grandfather, fought in World War II and died saving Helen's life. It's implied to be one of the reasons why Helen hired Will.
Even Evil Has Standards: Druit seemed to be working for the Nazis, though he was really working against them from within, or something (he did kill Hitler after all), because "I know evil when I see it."
Berserk Button: Threats to abnormals in general, and the Sanctuary and Magnus in particular. Discovering the murderer of his friend Father Jenson (who was the one who originally directed him to the Sanctuary) drove him into a berserker rage that allowed him to shrug off several bullets and strangle the man to death
Action Girl: Her personality didn't change much from her Cabal days, but she's still a pure good guy as shown when she is accidentally Brainwashed and Crazy one episode and still refuses to kill a man who would have been an Asshole Victim. While waiting for Magnus to retrieve the egg of a dangerous abnormal from the vault of a bank, she begins to case the joint apparently as a matter of habit. When the egg hatches and they need to quarantine the bank, her idea is to hold it up in order to prevent the other customers from leaving
Canada, Eh?: Although one episode established she grew up in Chicago, she moved to Montreal a few years after her father died, and the Sanctuary crew first meet up with her in Vancouver. She notably refers to Will in "Kali Part 1" as "Will Zed," as opposed to "Will Zee" as an American would.
Jerk with a Heart of Gold: she starts the show with a rather mercenary attitude, but by the beginning of season 3 she views the Sanctuary team as her family, and is particularly affectionate towards Will and Henry
They Changed It, Now It Sucks: An in-universe version. He got a movie deal for his superhero persona, but Executive Meddling basically retooled the entire concept. It made him so frustrated that he Took a Level in Jerkass and the suit rejected him when he tried to coax it off Kate. When it eventually did come back to him, he was convinced to go back to his roots and directed the film himself.