Former Hair Hunt
- Badass Army This entire generation of soldiers is described as such, having conquered almost all the known world in four days.
- Older Than They Look: They've been put in cryogenic sleep chambers for 100 years so however old they appear to be, add 100 years to that.
Tsuru Tsurulina III/Czar Baldy Bald III
- Big Bad: Of the Former Hair Hunters arc and Shinsetsu.
- Cherry Tapping: Ultimately defeated by Bo-bobo giving him a light chop to the armpit in Shinsetsu, after the standard arc-ending montage attack.
- Fantastic Racism: Vonsiders humans nothing but scum.
- Hollywood Cyborg: As it turns out
- Ignored Epiphany: After Bobobo goes to get efforts to show a technique to show him the wonders of humanity, he still decides to Take Over the World and make it worse than before.
- Kick the Dog: He has a roulette attack just to target Bobobo's friends one by one until they were destroyed.
- Kick Them While They Are Down: kills Bi-bibi and steals his Hair Ball after Bo-bobo has defeated him, paving the way for his rise to power and the sequel series.
- Knight of Cerebus: A misanthropic megalomanic whose first action against Bobobo is to immediately try and kill him, and decided to slowly kill off his friends once he finds out that he is a Living Macguffin. Not something you'd expect from odd series like this
- Lack of Empathy: When confronted by Bobobo after pushing his Berserk Button"Do you think I care?"
- Leitmotif: A subtle, but noticeable organ is played twice in his fight during his dark moments.
- Not Even Human: Upon absorbing the Hair Ball in the first fight with him, he reveals his true form to be a robotic demonic being.
- Our Robots Are Non Standard: Being an android and all.
- Robotic Reveal: The reason why we know he's one.
- No-Nonsense Nemesis: His reaction to any comedy is to ignore and continue to try to kill someone.
- Order Versus Chaos: seeks an entirely ordered world where those who do not obey his every command are dead.
- Person of Mass Destruction: in his introduction, he throws a few knives off into the distance. The result is something ripped right out of DragonBall.
- Ungrateful Bastard: A very notable example considering most of the villains become allies at this point Despite Bobobo sparing his life, he decides to screw everyone over by taking Bibibi's Hair Ball and reestablishing his empire.
- Vile Villain, Saccharine Show: He's a cybernetic, misanthropic megalomaniac with no hesitation in committing atrocities in order to maintain his authority. He stands out since most of the series involves light-hearted yet bizarre comedy, and is the sole reason after getting the Hair Ball that he manages to make the series Darker and Edgier with his new abilities.
Hanpen/General Lee Fishcake
Voiced by: Hiroaki Miura (JP), Steve Kramer (EN), Rais David Báscones (Spain, first apparitions), Juan Navarro (Spain, rest of the series)
- A Father to His Men: He certainly treats his allies with more respect than the main hero.
- Blood Knight: Although he was confident he was superior and later fights for his comrades, Hanpen likes a good fight.
- The Dragon: To the Third.
- Heel–Face Turn: After his defeat, he sides with Bobobo and the gang during the Hair Hunt Tournament.
- Redemption Demotion: Not as bad as Jelly Jiggler, but he's much less useful as a hero than he was as a villain.
- Social Darwinist: He harks on about the worthiness of his opponents.
- Bare Your Midriff: Male example.
- Crossdresser: Although unwillingly.Lambada:Why am I dressed like a colorblind prom date!?
- Cross-Dressing Voices: In the English dub.
- Determinator: According to Rem, he once had a stare-down with General Lee Fishcake until the General passed out.
- Jerk with a Heart of Gold
- Nice Hat
Jeda the Wind God
- Bare Your Midriff: She only wears a bra.
- Defeat Equals Friendship: As with a lot of Bobobo's foes, she turns good after being defeated.
- Heavy Sleeper: She struggles to start her battle because she can't stop falling asleep. Even in mid battle or MID-AIR she'll fall asleep.Rem: Just five more minutes...
- Heel–Face Turn: The entire latter half of Bobobo's battle with her involves melting her heart and turning her good.
- Meaningful Name: As in Rapid Eye Movement, a stage of sleeping.
- Power Makes Your Hair Grow: And gives you a jacket.
- Troubled Backstory Flashback: Apparently, she's actually the daughter of two beds. During her childhood, she was mocked by the other bed children.
- It's worse in the manga, she was BEATEN BY HER PARENTS.
- Stripperiffic: Yet no one really cares.
Combat Blues/Major Minor
- Covert Pervert: His two subordinates are two girls in swimsuits, and he's able to get back up if they cheer him on.
- Drill Sergeant Nasty: In the English dub, he speaks in a stereotypical drill sergeant fasion.
- Hidden Eyes: His helmet casts a shadow over his eyes.
- Nice Helmet
- Trap Master: He uses the Super Fist of Trapping.
Ujikin TOKIO/Not Nice Cream
- Beard of Evil
- Elemental Powers: Oddly enough, fire, in the form of the Sun, which leads to him being a Joke Character.
- Heel–Face Turn: In order to get past a guard, he sides with Don Patch and Beauty. This lasts about five minutes before a Face–Heel Turn.
- Joke Character: He's a bowl of shaved ice, and has the Fist of Summer. This does not end well for him.
- Kansai Regional Accent
Voiced by: Susumu Chiba (JP)
Voiced by: Koichi Tochika (JP)
Swimsuit Girl and Swimsuit GalThe subordinates of Major Minor, they are, as their names imply, a pair of girls wearing swimsuits.
- Heel–Face Turn: Though they weren't exactly evil to begin with, they're convinced by Bobobo to be good, without any hesitation from them.
- Nice Girls: Even when they work under Major Minor, they don't actually do anything to harm the heroes, they just play with each other in the pool and cheer on Major Minor when he needs their support. Bobobo convinces them to abandon the Major and fight for good extremely easily.
Voiced by: Kenta Miyake (JP)
- Even Evil Has Standards: When Don Patch fishes Jelly Jiggler back into the fight, Roman Samurai chastises him for breaking the rules.
Kancho-kun/Enema/The SplinterThe former commander of the Y-Block
- Ass Shove: Kancho-kun/The Splinter's specialty and does this Up to Eleven in the later story arcs...
- Did You Just Punch Out Cthulhu?: The fight with Hydrate was was very one sided till he does an epic Ass Shove to him.
- Heel–Face Turn: In the Reverse Margarita Arc.
Lububa of the Bubbles